124 research outputs found
Characterization of a fully active N-terminal 37-kDa polypeptide obtained by limited tryptic cleavage of pig kidney D-amino acid oxidase.
In order to obtain further information on the structure of D-amino acid oxidase (EC 1.4.3.3), limited proteolysis experiments have been carried out on its apo-, holo-, and holoenzyme-benzoate forms. The enzyme is unsensitive to 10% (w/w) chymotrypsin, while incubation with 10% (w/w) trypsin, under nondenaturating conditions, produces inactivation and proteolysis patterns which are different for the three forms of enzyme analyzed. These results confirm the previously reported conformational changes which occur upon binding of coenzyme to the apoprotein, and of benzoate to holoenzyme. The stable 37.0-kDa polypeptide, obtained from the apo- and holoenzyme-benzoate complex upon cleavage of a C-terminal 2.0-kDa fragment, retains full catalytic activity with unaltered kinetic parameters, and the coenzyme binding properties of the native enzyme. These results are in agreement with the tentative localization of the FAD-binding domain in the N-terminal region of the enzyme, and with the hypothesis that the function of the C-terminal region of D-amino acid oxidase could be related to the import of the enzyme into the peroxisomes, as suggested by Gould et al. (Gould, S. J., Keller, G. A., and Subramani, S. (1988) J. Cell. Biol. 107, 897-905)
The primary structure of the flavoprotein D-aspartate oxidase from beef kidney.
The complete primary structure of the peroxisomal flavoenzyme D-aspartate oxidase from beef kidney has been determined by analyses of the peptides obtained through fragmentation of the carboxymethylated protein with trypsin, CNBr, heptafluorobutyric acid/CNBr and Staphylococcus aureus V8 protease. The protein consists of a single polypeptide of 338 residues, accounting for a M(r) of 37,305 for the apoprotein. A form of the enzyme lacking Lys-338 and therefore ending with Pro-337 has been detected. The N-terminal residue is blocked. Seven cysteines and no disulfide bridges are present. Residue 228 can be either Ile or Val. Thus, D-aspartate oxidase presents two types of heterogeneity in the polypeptide chain in addition to the one already described concerning the possible content of FAD or 6-hydroxyflavin adenine dinucleotide. Comparison of the primary structure of D-aspartate oxidase with other known sequences reveals that D-aspartate oxidase is homologous with D-amino acid oxidase (another flavo-oxidase) and does not present significant sequence similarities with any other protein, including flavoenzymes
The Global Health Watch
Why three non-governmental organizations are launching an alternative to the World Health Repor
Conversion of nanoscale topographical information of cluster-assembled zirconia surfaces into mechanotransductive events promotes neuronal differentiation
Additional file 4: Table S1. Proteomic data for upregulated proteins. Proteins upregulated (compared to flat-Zr) or present only in cells grown on ns-Zr15. Adhesome proteins and proteins with roles in mechanobiological processes are marked in dark and light grey, respectively
Guidelines for the diagnosis, prevention and treatment of osteoporosis, 2012
La osteoporosis es una patologÃa en constante crecimiento y que afecta a más de 200 millones de personas a nivel mundial. Las recomendaciones presentes son guÃas para el diagnóstico, la prevención y tratamiento pero no normas para las decisiones clÃnicas en pacientes individuales. El médico debe adaptarlas a situaciones y pacientes deferentes, incorporando factores personales que trascienden los lÃmites de estas guÃas y hacen al saber y al arte del médico. Como todo conocimiento médico cientÃfico deben ser revisadas y actualizadas periódicamente a medida que se adquieran nuevas, mejores y más efectivas herramientas diagnósticas y terapéuticas.Osteoporosis is a constantly growing disease which affects over 200 million people worldwide. The present recommendations are guidelines for its diagnosis, prevention and treatment, but they do not constitute standards for clinical decisions in individual patients. The physician must adapt them to individual patients and special situations, incorporating personal factors that transcend the limits of these guidelines and are dependent on the knowledge and art of the physician. These guidelines should be reviewed and updated periodically as new, better and more effective diagnostic and therapeutic tools become available.Fil: Schurman, Leon. Grupo de Investigación en OsteopatÃas y Metabolismo Mineral; ArgentinaFil: Bagur, Alicia. Centro de OsteopatÃas Médicas; ArgentinaFil: Claus Hermberg, Heraldo. Hospital Alemán; ArgentinaFil: Messina, Osvaldo D.. Ministerio de Defensa. Ejercito Argentino. Hospital Militar Central Cirujano Mayor "Cosme Argerich"; ArgentinaFil: Negri, Armando L.. Universidad del Salvador; ArgentinaFil: Sánchez, Ariel. Centro de EndocrinologÃa; ArgentinaFil: González, Claudio. Centro de Educación Médica e Investigaciones ClÃnicas; ArgentinaFil: Diehl, MarÃa. Hospital Italiano de Buenos Aires; ArgentinaFil: Rey, Paula. Universidad del Salvador; ArgentinaFil: Gamba, Julieta. Ministerio de Defensa. Ejercito Argentino. Hospital Militar Central Cirujano Mayor "Cosme Argerich"; ArgentinaFil: Chiarpenello, Javier. Universidad Nacional de Rosario; ArgentinaFil: Moggia, MarÃa Susana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de ClÃnicas General San MartÃn; ArgentinaFil: Mastaglia, Silvina Rosana. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de InmunologÃa, Genética y Metabolismo; Argentin
Massimo Panebianco, Lo Stato post-globale. Ascesa e resistenza dello Stato globale Covid-free, Editoriale Scientifica, Napoli, 2020, (pp. 2-449)
Reflexiones en salud pública
Desde una visión general y práctica este texto facilita la reflexión sobre tópicos de interés para la salud pública. Con un lenguaje sencillo, busca aproximarse al estudio de la salud desde una visión interdisciplinar, partiendo del reconocimiento del rol que le compete al individuo, la sociedad y el Estado. El objetivo general es promover en el lector el análisis crÃtico de problemáticas relacionadas con el ámbito de la salud pública, constituyéndose en una iniciativa para el posterior desarrollo de acciones en el área
Erratum to nodal management and upstaging of disease. Initial results from the Italian VATS Lobectomy Registry
[This corrects the article DOI: 10.21037/jtd.2017.06.12.]
Correction to risk factors and impact of conversion from VATS to open lobectomy: analysis from a national database
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