Emerging unitary evolutions in dissipatively coupled systems

Having a broad range of methods available for implementing unitary operations is crucial for quantum information tasks. We study a dissipative process commonly used to describe dissipatively coupled systems and show that the process can lead to pure unitary dynamics on one part of a bipartite system, provided that the process is strong enough. As a consequence of these findings, we discuss within the framework of quantum control theory how the dissipative process can enable universal control of the considered part, thereby turning parts of the system into a system capable of universal quantum information tasks. We characterize the time scales necessary to implement gates with high fidelity through the dissipative evolution. The considered dissipative evolution is of particular importance since it can be engineered in the laboratory in the realm of superconducting circuits. Based on a reservoir that is formed by a lossy microwave mode we present a detailed study of how our theoretical findings can be realized in an experimental setting

A combined cryo-EM and molecular dynamics approach reveals the mechanism of ErmBL-mediated translation arrest

Nascent polypeptides can induce ribosome stalling, regulating downstream genes. Stalling of ErmBL peptide translation in the presence of the macrolide antibiotic erythromycin leads to resistance in Streptococcus sanguis. To reveal this stalling mechanism we obtained 3.6-angstrom-resolution cryo-EM structures of ErmBL-stalled ribosomes with erythromycin. The nascent peptide adopts an unusual conformation with the C-terminal Asp10 side chain in a previously unseen rotated position. Together with molecular dynamics simulations, the structures indicate that peptide-bond formation is inhibited by displacement of the peptidyl-tRNA A76 ribose from its canonical position, and by non-productive interactions of the A-tRNA Lys11 side chain with the A-site crevice. These two effects combine to perturb peptide-bond formation by increasing the distance between the attacking Lys11 amine and the Asp10 carbonyl carbon. The interplay between drug, peptide and ribosome uncovered here also provides insight into the fundamental mechanism of peptide-bond formation

Structure of the mammalian antimicrobial peptide Bac7(1-16) bound within the exit tunnel of a bacterial ribosome

Proline-rich antimicrobial peptides (PrAMPs) produced as part of the innate immune response of animals, insects and plants represent a vast, untapped resource for the treatment of multidrug-resistant bacterial infections. PrAMPs such as oncocin or bactenecin-7 (Bac7) interact with the bacterial ribosome to inhibit translation, but their supposed specificity as inhibitors of bacterial rather than mammalian protein synthesis remains unclear, despite being key to developing drugs with low toxicity. Here, we present crystal structures of the Thermus thermophilus 70S ribosome in complex with the first 16 residues of mammalian Bac7, as well as the insect-derived PrAMPs metalnikowin I and pyrrhocoricin. The structures reveal that the mammalian Bac7 interacts with a similar region of the ribosome as insect-derived PrAMPs. Consistently, Bac7 and the oncocin derivative Onc112 compete effectively with antibiotics, such as erythromycin, which target the ribosomal exit tunnel. Moreover, we demonstrate that Bac7 allows initiation complex formation but prevents entry into the elongation phase of translation, and show that it inhibits translation on both mammalian and bacterial ribosomes, explaining why this peptide needs to be stored as an inactive pro-peptide. These findings highlight the need to consider the specificity of PrAMP derivatives for the bacterial ribosome in future drug development efforts

Present-day surface deformation of the Alpine region inferred from geodetic techniques

We provide a present-day surface-kinematics model for the Alpine region and surroundings based on a high-level data analysis of about 300 geodetic stations continuously operating over more than 12 years. This model includes a deformation model, a continuous surface-kinematic (velocity) field, and a strain field consistently assessed for the entire Alpine mountain belt. Special care is given to the use of the newest Global Navigation Satellite Systems (GNSS) processing standards to determine high-precision 3-D station coordinates. The coordinate solution refers to the reference frame IGb08, epoch 2010.0. The mean precision of the station positions at the reference epoch is ±1.1&thinsp;mm in N and E and ±2.3&thinsp;mm in height. The mean precision of the station velocities is ±0.2&thinsp;mm&thinsp;a−1 in N and E and ±0.4&thinsp;mm&thinsp;a−1 in height. The deformation model is derived from the point-wise station velocities using a geodetic least-squares collocation (LSC) approach with empirically determined covariance functions. According to our results, no significant horizontal deformation is detected in the Western Alps, while across the Southern and Eastern Alps the deformation vectors describe a progressive eastward rotation towards Pannonia. This kinematic pattern also makes evident an increasing magnitude of the deformation from 0.1&thinsp;mm&thinsp;a−1 in the western part of Switzerland up to about 1.3&thinsp;mm&thinsp;a−1 in the Austrian Alps. The largest shortening is observed along the southern front of the Eastern Alps (in the northern area of the Venetian-Friuli Basin) and in the northern part of the Apennine Peninsula, where rates reach 2 and 3&thinsp;mm&thinsp;a−1, respectively. The average accuracy of the horizontal deformation model is ±0.2&thinsp;mm&thinsp;a−1. Regarding the vertical kinematics, our results clearly show an ongoing average uplift rate of 1.8&thinsp;mm&thinsp;a−1 of the entire mountain chain, with the exception of the southern part of the Western Alps, where no significant uplift (less than 0.5&thinsp;mm&thinsp;a−1) is detected. The fastest uplift rates (more than 2&thinsp;mm&thinsp;a−1) occur in the central area of the Western Alps, in the Swiss Alps, and in the Southern Alps in the boundary region between Switzerland, Austria, and Italy. The general uplift observed across the Alpine mountain chain decreases towards the outer regions to stable values between 0.0 and 0.5&thinsp;mm&thinsp;a−1 and, in some cases, to subsidence like in the Liguro-Provençal and Vienna basins, where vertical rates of −0.8 and −0.3&thinsp;mm&thinsp;a−1 are observed, respectively. In the surrounding region, three regional subsidence regimes are identified: the Rhône-Bresse Graben with −0.8&thinsp;mm&thinsp;a−1, the Rhine Graben with −1.3&thinsp;mm&thinsp;a−1, and the Venetian-Friuli Basin with −1.5&thinsp;mm&thinsp;a−1. The estimated uncertainty of our vertical motion model across the Alpine mountain belt is about ±0.3&thinsp;mm&thinsp;a−1. The strain field inferred from the deformation model shows two main contrasting strain regimes: (i) shortening across the south-eastern front of the Alps and the northern part of the Dinarides and (ii) extension in the Apennines. The pattern of the principal strain axes indicates that the compression directions are more or less perpendicular to the thrust belt fronts, reaching maximum values of 20×10−9&thinsp;a−1 in the Venetian-Friuli and Po basins. Across the Alpine mountain belt, we observe a slight dilatation regime in the Western Alps, which smoothly changes to a contraction regime in western Austria and southern Germany, reaching maximum shortening values of 6×10−9&thinsp;a−1 in north-eastern Austria. The numerical results of this study are available at https://doi.pangaea.de/10.1594/PANGAEA.886889.</p

Structural and mechanistic basis for translation inhibition by macrolide and ketolide antibiotics

Macrolides and ketolides comprise a family of clinically important antibiotics that inhibit protein synthesis by binding within the exit tunnel of the bacterial ribosome. While these antibiotics are known to interrupt translation at specific sequence motifs, with ketolides predominantly stalling at Arg/Lys-X-Arg/Lys motifs and macrolides displaying a broader specificity, a structural basis for their context-specific action has been lacking. Here, we present structures of ribosomes arrested during the synthesis of an Arg-Leu-Arg sequence by the macrolide erythromycin (ERY) and the ketolide telithromycin (TEL). Together with deep mutagenesis and molecular dynamics simulations, the structures reveal how ERY and TEL interplay with the Arg-Leu-Arg motif to induce translational arrest and illuminate the basis for the less stringent sequence-specific action of ERY over TEL. Because programmed stalling at the Arg/Lys-X-Arg/Lys motifs is used to activate expression of antibiotic resistance genes, our study also provides important insights for future development of improved macrolide antibiotics

Effect of the four-sheet Fermi surface on magnetoresistivity of MgB2

Recent experimental data of anisotropic magnetoresistivity measured in MgB2 films have shown an intriguing behaviour: the angular dependence of magnetoresistivity changes dramatically with temperature and disorder. In order to explain such phenomenology, in this work, we extend our previous analyses on multiband transverse magnetoresistivity in magnesium diboride, by calculating its analytic expression, assuming a constant anisotropic Fermi surface mass tensor. The calculation is done for arbitrary orientation of the magnetic field with respect to the crystalline axes and for the current density either perpendicular or parallel to the magnetic field. This approach allows to extract quite univocally the values of the scattering times in the s- and p- bands by fitting experimental data with a simple analytic expression. We also extend the analysis to the magnetoresistivity of polycrystalline samples, with an arbitrary angle between the current density and the magnetic field, taking into account the anisotropy of each randomly oriented grain. Thereby, we propose magnetoresistivity as a very powerful characterization tool to explore the effect of disorder by irradiation or selective doping as well as of phonon scattering in each one of the two types of bands, in single crystals and polycrystalline samples, which is a crucial issue in the study of magnesium diboride.Comment: 25 pages, 4 figures. in press on The European Physical Journal

Elastic properties of B-C-N films grown by N2-reactive sputtering from boron carbide targets

The following article appeared in Journal of Applied Physics 114.21 (2013): 213508 and may be found at http://scitation.aip.org/content/aip/journal/jap/114/21/10.1063/1.4837655Boron-carbon-nitrogen films were grown by RF reactive sputtering from a B4C target and N2 as reactive gas. The films present phase segregation and are mechanically softer than boron carbide films (a factor of more than 2 in Young's modulus). This fact can turn out as an advantage in order to select buffer layers to better anchor boron carbide films on substrates eliminating thermally induced mechanical tensions.This work has been supported by Spanish MINECO under contracts MAT2009-08786 and MAT2012-37276- C03-01 as well as by the Madrid Regional Government though contract S2009/MAT-1756