Characterization of a nuclear localization signal in the foot-and-mouth disease virus polymerase

AbstractWe have experimentally tested whether the MRKTKLAPT sequence in FMDV 3D protein (residues 16 to 24) can act as a nuclear localization signal (NLS). Mutants with substitutions in two basic residues within this sequence, K18E and K20E, were generated. A decreased nuclear localization was observed in transiently expressed 3D and its precursor 3CD, suggesting a role of K18 and K20 in nuclear targeting. Fusion of MRKTKLAPT to the green fluorescence protein (GFP) increased the nuclear localization of GFP, which was not observed when GFP was fused to the 3D mutated sequences. These results indicate that the sequence MRKTKLAPT can be functionally considered as a NLS. When introduced in a FMDV full length RNA replacements K18E and K20E led to production of revertant viruses that replaced the acidic residues introduced (E) by K, suggesting that the presence of lysins at positions 18 and 20 of 3D is essential for virus multiplication

The Spanish National Parks, a catalyst for sustainable tourism

Los Parques Nacionales españoles son espacios naturales protegidos caracterizados por su alto valor natural y cultural. En estos espacios confluyen intereses conservacionistas, sociales y económicos que exigen estrategias innovadoras de turismo sostenible y marketing relacional. Estas estrategias deben basarse en el consenso de los agentes implicados para hacer compatible la conservación de estos entornos privilegiados, su disfrute por parte de los visitantes y el desarrollo económico de la población local. The Spanish National Parks are characterized by their high natural and cultural value. Conservacionist, social and economic interests meet in these protected areas, that require innovative strategies of sustainable tourism and relationship marketing. These strategies should be based on the involved agents consensus to make compatible the conservation of these privileged spaces with visitors´ enjoyment and the local economic development

A digital-based integrated methodology for the preventive conservation of cultural heritage: the experience of HeritageCare project

A sustainable conservation strategy for cultural heritage protection is not feasible without a systematic documentation, registration and management of the information. The adoption of integrated inspection protocols and regular tracking processes, based on standardized procedures and uniform criteria, are the basis to successfully replace actual curative strategies with proactive conservation approaches. The opportunities brought by the digital tools can offer tremendous advantages in this regard. This paper explores the leading role that digitization is assuming in the context of heritage conservation through the experience of the HeritageCare project - Monitoring and preventive conservation of historic and cultural heritage" (SOE1/P5/P0258). The project has developed a digital-based integrated methodology aimed at providing enhanced tools and services to properly document cultural heritage buildings and engage directly owners in the conservation process of their legacy. The structured digital workflow on which the HeritageCare protocol relies is described in detail, encompassing different levels of information. Finally, the full application of the protocol is presented with reference to one of the most emblematic case studies of the project, the Ducal Palace of Guimaraes, Portugal.- This work was partly financed by FEDER funds through the Competitiveness Factors Operational Programme (COMPETE) and by national funds through the Foundation for Science and Technology (FCT) within the scope of project POCI-01-0145-FEDER-007633

Antimicrobial and bactericidal impacts of Bacillus amyloliquefaciens CECT 5940 on fecal shedding of pathogenic bacteria in dairy calves and adult dogs

The administration of B. amyloliquefaciens CECT 5940 to the dog diet has no significant effect on the hardness of the stool. Meanwhile, the bacillus count increases while the coliforms count decreases upon B. amyloliquefaciens CECT 5940 administration. This reveals that B. amyloliquefaciens CECT 5940 survived the gastrointestinal passage and rapidly colonized the dog intestine, which could positively affect the metabolism and composition of the intestinal microflora. These results show that B. amyloliquefaciens are a promising probiotic with an antimicrobial and bactericidal activities against the intestinal pathogenic bacteria for dairy calves and adult dogs.Two experiments were carried out to evaluate the bactericidal impacts of Bacillus amyloliquefaciens CECT 5940 on the shedding of faecal pathogenic bacteria in dairy calves (Experiment 1) and in adults dogs (experiment 2). In the calves experiment, a completely randomized design was used to investigate the faecal bacteria profile of Holstein dairy calves fed with either pasteurized waste milk (PWM; n = 9) or a formulated non-medicated milk replacer (NMR; n = 9) for 60 d. The NMR containing sodium-butyrate and the active probiotic B. amyloliquefaciens CECT 5940. In the dogs experiment, addition of same probiotic (i.e., B. amyloliquefaciens CECT 5940) was carried out in two stages. The first stage started from day 7–37, and the second from day 44–71. The assessment of faecal score measured on day 22, 37, 42, 57, 71 and 77 to determine the texture of the stools. Calves received PWM consumed (P < 0.05) more starter feed between day 16 and day 45. The calves fed NMR had more moisture faeces and less cough reflux than the PWM-calves. Feeding NMR to calves increased faecal Klebsiella oxytoca and Proteus vulgaris counts in comparison to PWM-calves

FarmConners market showcase results: wind farm flow control considering electricity prices

The EU and UK have made ambitious commitments under the net-zero plans to decarbonise their economies by 2050. For this, offshore wind will play a major role, significantly contributing to a paradigm shift in the power generation and greater volatility of electricity prices. The operating strategy of wind farms should therefore move from power maximisation to profit maximisation which includes income from providing power system services and the reduction of maintenance costs. Wind farm flow control (WFFC) is a key enabler for this shift through mitigation of wake effects in the design and operation phases. The results of the FarmConners market showcases presented here are the first attempt to economically assess WFFC strategies with respect to electricity market prices. Here, we present a conceptual simulation study starting from individual turbine control and extend it to layouts with 10 and 32 turbines operated with WFFC based on the results of five participants. Each participant belonged to a different research group with their respective simulation environments, flow models and WFFC strategies. Via a comparative analysis of relative WFFC benefits estimated per participant, the implications of wind farm size, the applied control strategy and the overall model fidelity are discussed in zero-subsidy scenarios. For all the participants, it is seen that the income gain can differ significantly from the power gain depending on the electricity price under the same inflow, and a favourable control strategy for dominant wind directions can pay off even for low electricity prices. However, a strong correlation between income and power gain is also observed for the analysed high-electricity-price scenarios, underlining the need for additional modelling capabilities to carry out a more comprehensive value optimisation including lower prices and system requirements driven cases.FarmConners market showcase results: wind farm flow control considering electricity pricespublishedVersio

Sexuality and Religious Ethics: Analysis in a Multicultural University Context

Sexuality is still perceived by some cultures as a taboo subject. Although there is now a more open attitude towards sexuality, the maintenance of virginity is one of the most concerning issues in some religions. The aim of this research is to investigate the sexual behavior of university students and analyze how culture and religion influence the beliefs and maintenance of virginity in women and men. A mixed methodology was used, involving 355 students in the quantitative design and 18 informants for the qualitative study who took part in two focus groups. The results indicate that religion and the degree of religious practice are predictor variables for the decision to have penetrative sex, with Muslim women and men giving more importance to the maintenance of a woman’s virginity. However, this hymen-centric view does not prevent other sexual practices, such as oral and/or anal sex, among young people who wish to maintain their virginity until marriage. Religious ethics continue to influence the sexual behavior of young people today. Therefore, nursing education must address these issues to improve the affective sexual health of the population.Partial funding for open access charge: Universidad de Málag

Topoisomerase II inhibitors induce DNA damage-dependent interferon responses circumventing Ebola virus immune evasion

Ebola virus (EBOV) protein VP35 inhibits production of interferon alpha/beta (IFN) by blocking RIG-I-like receptor signaling pathways, thereby promoting virus replication and pathogenesis. A high-throughput screening assay, developed to identify compounds that either inhibit or bypass VP35 IFN-antagonist function, identified five DNA intercalators as reproducible hits from a library of bioactive compounds. Four, including doxorubicin and daunorubicin, are anthracycline antibiotics that inhibit topoisomerase II and are used clinically as chemotherapeutic drugs. These compounds were demonstrated to induce IFN responses in an ATM kinase-dependent manner and to also trigger the DNA-sensing cGAS-STING pathway of IFN induction. These compounds also suppress EBOV replication in vitro and induce IFN in the presence of IFN-antagonist proteins from multiple negative-sense RNA viruses. These findings provide new insights into signaling pathways activated by important chemotherapy drugs and identify a novel therapeutic approach for IFN induction that may be exploited to inhibit RNA virus replication

Fundamentos de imposición a las rentas empresariales

El Libro Fundamentos de Imposición a las Rentas Empresariales, es el resultado del trabajo en equipo de distintos colaboradores de la SUNAT, quienes a partir del ejercicio de investigación documental comparten doctrina asequible para estudiantes de pregrado. Se privilegió las referencias a los pronunciamientos de la SUNAT y la jurisprudencia de observancia obligatoria del Tribunal Fiscal a fin de establecer un marco inicial de referencia sobre la forma de aplicar la normativa para casos específicos

Glossary of Geography

El Glosario de Geografía que a continuación se presenta constituye la primera parte de un proyecto más amplio. La idea fundamental de este proyecto plurianual es proporcionar a los alumnos del Grado de Geografía y Ordenación del Territorio de la Universidad Complutense una herramienta que pueda ser útil y de fácil manejo para la comprensión de los principales conceptos de Geografía, en las diferentes materias que se abordan, y a la vez que también pueda ser de utilidad para los profesores, porque la información gráfica que acompaña a casi todos los términos del Glosario puede ayudar a la explicación de esos conceptos. Esta primera parte del Glosario se ha centrado en los términos de las materias de Climatología, Cartografía y Geomorfología, por considerar que tanto el volumen de términos propios de las mismas, como su posición en los primeros cursos del Plan de Estudios, lo justifican. Evidentemente, no se pretende abarcar todos los términos propios de estas ciencias, sino los de mayor uso en cada materia concreta. Se ampliará en cursos próximos abarcandoThe Geography Glossary that follows is the first part of a larger project. The fundamental idea of ​​this multiannual project is to provide students of the Degree in Geography and Territorial Planning of the Complutense University with a tool that can be useful and easy to use for understanding the main concepts of Geography, in the different subjects that are address, and at the same time may be useful for teachers, because the graphic information that accompanies almost all the terms of the Glossary can help explain those concepts. This first part of the Glossary has focused on the terms of the subjects of Climatology, Cartography and Geomorphology, considering that both the volume of their own terms and their position in the first courses of the Study Plan justify it. Obviously, it is not intended to cover all the terms of these sciences, but those of greater use in each specific subject. It will be expanded in upcoming courses covering other subjects or increasing the number of terms already addressed.Depto. de GeografíaFac. de Geografía e HistoriaFALSEsubmitte