Dendrochronological Analysis of Wood from the Num-Khibya-Sikheri VIа Site

The article deals with the dendrochronological analysis of the archaeological wood collection from the Num-Khibya-Sikheri VIa site, a burial complex belonging to the Northern Selkup cultural tradition and located on the territory of the Taz Polar region (Tazovskiy district of the YNAO). The dates of the complex existence, obtained within the framework of the tree-ring analysis, fall within the time interval from the second quarter of the 19th century to the beginning of the 20th century. This in turn confirms and significantly supplements the previously discovered archival-recorded evidence of the Selkup fishing farms emergence in the lower Taz (which fall on the upper date limit of the complex). The received lower date of the complex functioning is the earliest proven borderline of the presence of the northern Selkups on the territory of the Taz Arctic

Redetermination of bis­(O,O′-diethyl dithio­phosphato-κ2 S,S′)nickel(II)

The centrosymmetric title complex, [Ni{S2P(OC2H5)2}2], has been redetermined using area-detector data. The central Ni(S2P)2 core is essentially planar and confirms the early results of McConnell & Kastalsky [Acta Cryst. (1967), 22, 853–859] based on multiple film technique data. In the title structure, the standard uncertainty values are approximately seven times lower and all H-atom positions are calculated. A pair of short symmetry-related H⋯H contacts with distances of 2.33 Å is observed in the crystal structure

The effects of entrepreneurship education

Entrepreneurship education ranks high on policy agendas in Europe and the US, but little research is available to assess its impact. To help close this gap we investigate whether entrepreneurship education a?ects intentions to be entrepreneurial uniformly or whether it leads to greater sorting of students. The latter can reduce the average intention to be entrepreneurial and yet be socially beneficial. This paper provides a model of learning in which entrepreneurship education generates signals to students. Drawing on the signals, students evaluate their aptitude for entrepreneurial tasks. The model is tested using data from a compulsory entrepreneurship course. Using ex ante and ex post survey responses from students, we find that intentions to found decline somewhat although the course has significant positive e?ects on students’ self-assessed entrepreneurial skills. The empirical analysis supports the hypothesis that students receive informative signals and learn about their entrepreneurial aptitude. We outline implications for educators and public policy

Hemoglobin is an oxygen-dependent glutathione buffer adapting the intracellular reduced glutathione levels to oxygen availability

Fast changes in environmental oxygen availability translate into shifts in mitochondrial free radical production. An increase in intraerythrocytic reduced glutathione (GSH) during deoxygenation would support the detoxification of exogenous oxidants released into the circulation from hypoxic peripheral tissues. Although reported, the mechanism behind this acute oxygen-dependent regulation of GSH in red blood cells remains unknown. This study explores the role of hemoglobin (Hb) in the oxygen-dependent modulation of GSH levels in red blood cells. We have demonstrated that a decrease in Hb O2 saturation to 50% or less observed in healthy humans while at high altitude, or in red blood cell suspensions results in rising of the intraerythrocytic GSH level that is proportional to the reduction in Hb O2 saturation. This effect was not caused by the stimulation of GSH de novo synthesis or its release during deglutathionylation of Hb's cysteines. Using isothermal titration calorimetry and in silico modeling, we observed the non-covalent binding of four molecules of GSH to oxy-Hb and the release of two of them upon deoxygenation. Localization of the GSH binding sites within the Hb molecule was identified. Oxygen-dependent binding of GSH to oxy-Hb and its release upon deoxygenation occurred reciprocally to the binding and release of 2,3-bisphosphoglycerate. Furthermore, noncovalent binding of GSH to Hb moderately increased Hb oxygen affinity. Taken together, our findings have identified an adaptive mechanism by which red blood cells may provide an advanced antioxidant defense to respond to oxidative challenges immediately upon deoxygenation

Reduced purine biosynthesis in humans after their divergence from Neandertals

We analyze the metabolomes of humans, chimpanzees, and macaques in muscle, kidney and three different regions of the brain. Although several compounds in amino acid metabolism occur at either higher or lower concentrations in humans than in the other primates, metabolites downstream of adenylosuccinate lyase, which catalyzes two reactions in purine synthesis, occur at lower concentrations in humans. This enzyme carries an amino acid substitution that is present in all humans today but absent in Neandertals. By introducing the modern human substitution into the genomes of mice, as well as the ancestral, Neandertal-like substitution into the genomes of human cells, we show that this amino acid substitution contributes to much or all of the reduction of de novo synthesis of purines in humans

Cosmic ray oriented performance studies for the JEM-EUSO first level trigger

JEM-EUSO is a space mission designed to investigate Ultra-High Energy Cosmic Rays and Neutrinos (E > 5 ⋅ 1019 eV) from the International Space Station (ISS). Looking down from above its wide angle telescope is able to observe their air showers and collect such data from a very wide area. Highly specific trigger algorithms are needed to drastically reduce the data load in the presence of both atmospheric and human activity related background light, yet retain the rare cosmic ray events recorded in the telescope. We report the performance in offline testing of the first level trigger algorithm on data from JEM-EUSO prototypes and laboratory measurements observing different light sources: data taken during a high altitude balloon flight over Canada, laser pulses observed from the ground traversing the real atmosphere, and model landscapes reproducing realistic aspect ratios and light conditions as would be seen from the ISS itself. The first level trigger logic successfully kept the trigger rate within the permissible bounds when challenged with artificially produced as well as naturally encountered night sky background fluctuations and while retaining events with general air-shower characteristics

Regularities of Microstructure Evolution in a Cu-Cr-Zr Alloy during Severe Plastic Deformation

The effect of severe plastic deformation by the conforming process of equal channel angular extrusion (ECAE-Conform) followed by cold rolling on the microstructures developed in a Cu-0.1Cr-0.1Zr alloy was investigated. Following the ECAE-Conform of 1 to 8 passes (corresponding strains were 0.8 to 6.4) cold rolling to a total strain of 4 was accompanied by substantial grain refinement and strengthening. An average grain size tended to approach 160 nm with an increase in the rolling reduction. An increase in the ECAE-Conform strain promoted the grain refinement during subsequent cold rolling. The fraction of the ultrafine grains with a size of 160 nm after cold rolling to a strain of 4 increased from 0.12 to 0.52 as the number of ECAE-Conform passes increased from 1 to 8. Correspondingly, the yield strength increased above 550 MPa. The strengthening could be expressed by a Hall–Petch type relationship with a grain size strengthening factor of 0.11 MPa m0.5

Shorter Chain Triglycerides Are Negatively Associated with Symptom Improvement in Schizophrenia

Schizophrenia is a serious mental disorder requiring lifelong treatment. While medications are available that are effective in treating some patients, individual treatment responses can vary, with some patients exhibiting resistance to one or multiple drugs. Currently, little is known about the causes of the difference in treatment response observed among individuals with schizophrenia, and satisfactory markers of poor response are not available for clinical practice. Here, we studied the changes in the levels of 322 blood plasma lipids between two time points assessed in 92 individuals diagnosed with schizophrenia during their inpatient treatment and their association with the extent of symptom improvement. We found 20 triglyceride species increased in individuals with the least improvement in Positive and Negative Syndrome Scale (PANSS) scores, but not in those with the largest reduction in PANSS scores. These triglyceride species were distinct from the rest of the triglyceride species present in blood plasma. They contained a relatively low number of carbons in their fatty acid residues and were relatively low in abundance compared to the principal triglyceride species of blood plasma

Time-Dependent Effect of Sciatic Nerve Injury on Rat Plasma Lipidome

Neuropathic pain is a condition affecting the quality of life of a substantial part of the population, but biomarkers and treatment options are still limited. While this type of pain is caused by nerve damage, in which lipids play key roles, lipidome alterations related to nerve injury remain poorly studied. Here, we assessed blood lipidome alterations in a common animal model, the rat sciatic nerve crush injury. We analyzed alterations in blood lipid abundances between seven rats with nerve injury (NI) and eight control (CL) rats in a time-course experiment. For these rats, abundances of 377 blood lipid species were assessed at three distinct time points: immediately after, two weeks, and five weeks post injury. Although we did not detect significant differences between NI and CL at the first two time points, 106 lipids were significantly altered in NI five weeks post injury. At this time point, we found increased levels of triglycerides (TGs) and lipids containing esterified palmitic acid (16:0) in the blood plasma of NI animals. Lipids containing arachidonic acid (20:4), by contrast, were significantly decreased after injury, aligning with the crucial role of arachidonic acid reported for NI. Taken together, these results indicate delayed systematic alterations in fatty acid metabolism after nerve injury, potentially reflecting nerve tissue restoration dynamics