Levantamento de ciclopídeos (Crustacea, Copepoda) no Brasil e avaliação preliminar de seu potencial como predadores dos vetores da dengue

INTRODUCTION: Cyclopid copepods are known to be good mosquito controllers, specially as regards the larvae of the dengue vectors Aedes aegypti and Ae. albopictus. MATERIAL AND METHOD: The objective of the study was to survey the local copepod fauna and search for new strains of M. longisetus var. longisetus, comparing the potential of the samples found with the current strain ML-01 against Ae. albopictus larvae, under laboratory conditions. Eleven bodies of water in Campinas, SP, Brazil, were screened for copepods by collecting 1.5 l of water from each of then. The predatory potential of adults copepods was evaluated over 24 h, in the laboratory, for groups of 5 individuals preying upon 30 first instar Ae. albopictus larvae. RESULTS AND CONCLUSION: The following cyclopid species were found: Metacyclops mendocinus, Tropocyclops prasinus, Eucyclops sp, Eucyclops serrulatus, Eucyclops solitarius, Eucyclops ensifer, Macrocyclops albidus var. albidus and Mesocyclops longisetus var. longisetus. The predatory potential of these copepods ranged from nil to 97.3%. A sample collected in the field containing only M. longisetus var. longisetus showed the best control efficiency with no significant difference from a three-year old laboratory culture (ML-01) of the same species evaluated for comparison. The sample with few M. albidus var. albidus was ranked in second place showing an average 25.9% efficiency. The use of copepods in trap tires as dengue vector controllers is discussed.INTRODUÇÃO: Copépodos ciclopídeos são conhecidos como bons controladores de mosquitos, especialmente quando considerado as larvas dos vetores da dengue Aedes aegypti e Ae. albopictus. MATERIAL E MÉTODO: Onze corpos d'água em Campinas, SP, Brasil, foram avaliados para copépodos coletando-se 1,5 l de água de cada um deles. O potencial predador dos copépodos adultos foi avaliado por 24 h, em laboratório, em grupos de 5 indivíduos predadando sobre 30 larvas de 1° estádio de Ae. albopictus. RESULTADOS E CONCLUSÕES: No presente levantamento as seguintes espécies de ciclopídeos foram encontradas: Metacyclops mendocinus, Tropocyclops prasinus, Eucyclops sp, Eucyclops serrulatus, Eucyclops solitarius, Eucyclops ensifer, Macrocyclops albidus var. albidus and Mesocyclops longisetus var. longisetus. O potencial predador desses copépodos variou de zero a 97,3%. A amostra coletada no campo contendo apenas com M. longisetus var. longisetus mostrou a melhor eficiência de controle, sem diferença significativa de uma cultura de laboratório (ML-01) criada por 3 anos, desta mesma espécie, que foi avaliada para comparação. A amostra com poucos M. albidus var. albidus foi cotada em segundo melhor lugar, apresentando em média 25,9% de eficiência. O uso de copépodos em pneus armadilha como controladores dos vetores da dengue é discutido.22122

Levantamento de ciclopídeos (Crustacea, Copepoda) no Brasil e avaliação preliminar de seu potencial como predadores dos vetores da dengue

INTRODUCTION: Cyclopid copepods are known to be good mosquito controllers, specially as regards the larvae of the dengue vectors Aedes aegypti and Ae. albopictus. MATERIAL AND METHOD: The objective of the study was to survey the local copepod fauna and search for new strains of M. longisetus var. longisetus, comparing the potential of the samples found with the current strain ML-01 against Ae. albopictus larvae, under laboratory conditions. Eleven bodies of water in Campinas, SP, Brazil, were screened for copepods by collecting 1.5 l of water from each of then. The predatory potential of adults copepods was evaluated over 24 h, in the laboratory, for groups of 5 individuals preying upon 30 first instar Ae. albopictus larvae. RESULTS AND CONCLUSION: The following cyclopid species were found: Metacyclops mendocinus, Tropocyclops prasinus, Eucyclops sp, Eucyclops serrulatus, Eucyclops solitarius, Eucyclops ensifer, Macrocyclops albidus var. albidus and Mesocyclops longisetus var. longisetus. The predatory potential of these copepods ranged from nil to 97.3%. A sample collected in the field containing only M. longisetus var. longisetus showed the best control efficiency with no significant difference from a three-year old laboratory culture (ML-01) of the same species evaluated for comparison. The sample with few M. albidus var. albidus was ranked in second place showing an average 25.9% efficiency. The use of copepods in trap tires as dengue vector controllers is discussed.INTRODUÇÃO: Copépodos ciclopídeos são conhecidos como bons controladores de mosquitos, especialmente quando considerado as larvas dos vetores da dengue Aedes aegypti e Ae. albopictus. MATERIAL E MÉTODO: Onze corpos d'água em Campinas, SP, Brasil, foram avaliados para copépodos coletando-se 1,5 l de água de cada um deles. O potencial predador dos copépodos adultos foi avaliado por 24 h, em laboratório, em grupos de 5 indivíduos predadando sobre 30 larvas de 1° estádio de Ae. albopictus. RESULTADOS E CONCLUSÕES: No presente levantamento as seguintes espécies de ciclopídeos foram encontradas: Metacyclops mendocinus, Tropocyclops prasinus, Eucyclops sp, Eucyclops serrulatus, Eucyclops solitarius, Eucyclops ensifer, Macrocyclops albidus var. albidus and Mesocyclops longisetus var. longisetus. O potencial predador desses copépodos variou de zero a 97,3%. A amostra coletada no campo contendo apenas com M. longisetus var. longisetus mostrou a melhor eficiência de controle, sem diferença significativa de uma cultura de laboratório (ML-01) criada por 3 anos, desta mesma espécie, que foi avaliada para comparação. A amostra com poucos M. albidus var. albidus foi cotada em segundo melhor lugar, apresentando em média 25,9% de eficiência. O uso de copépodos em pneus armadilha como controladores dos vetores da dengue é discutido

Experimental Infection of Aedes albopictus (Diptera: Culicidae) Larvae with the Xiphidiocercariae of a Hematolechid

Aedes albopictus larvae were exposed, either individually or in groups, to different concentrations of xiphidiocercariae of Haematoloechus sp. for parasitological studies. It was observed the acute lethal effect and some aspects of the host-parasite relationship, such as delay or progress in the host life cycle, the number and location of the metacercariae in the host, adult host malformations and the amount of metacercariae required to cause death. A delay in the cycle and a high mortality rate was in general observed. Inside the larvae, the metacercariae were found predominantly in the thorax, abdominal segments and in the head, along with a reduced number in the anal lobe and cervix. It was shown that in addition to the quantity of metacercariae present, their location in the larvae was also relevant in the determination of mortality and anomalies. Malformed adults developed from larvae containing from one to three metacercariae.57357

Susceptibilidade de populações de Simulium (Chirostilbia) pertinax Kollar, 1832 (Culicomorpha, Simuliidae) ao temephos e a um formulado à base de Bacillus thuringiensis var. israelensis

The use of wooden troughs on stream beds, artificially colonized by blackfly larvae, is proposed for larvicide evaluations. Mortality was recorded 3 or 4 hours after treatment. Larval susceptibility was also evaluated utilizing the LT50 criterion. In there field assays Simulium (C.) pertinax populations from the litoral of S. Paulo and Rio de Janeiro States were shown to be resistant to temephos, even when subjected to high concentrations. Vectobac 12 AS, a Bacillus thuringiensis var. israelensis product, was shown to be more potent against late instar larvae and efficient in concentrations higher than 7,200 ITU/1 (10 min). The LT50 to 3,744 ITU/1 (10 min) was calculated as 70.9 min.O uso de pequenas calhas de madeira no leito de riachos, manualmente colonizadas com borrachudos, é proposto para avaliações da eficiência de larvicidas 3 ou 4 horas após as aplicações. A susceptibilidade larval também foi avaliada pelo critério de TL50. Em três ensaios no litoral do Estado de São Paulo e Rio de Janeiro, populações de Simulium (C.) pertinax Kollar, 1832 mostraram-se resistentes ao temephos, mesmo em altas concentrações. Vectobac 12 AS, à base de Bacillus thuringiensis var. israelensis mostrou-se mais potente contra larvas dos últimos estádios e eficiente para controle em concentrações a partir de 7.200 UIP/1 (10 min.). O TL50para 3.744 UIP/1 (10 min.) foi de 70,9 minutos

Susceptibility of population of Simulium (Chirostilbia) pertinax Kollar, 1832 (Culicomorpha, Simuliidae) to temephos and Bacillus thuringiensis var. israelensis formulation

The use of wooden troughs on stream beds, artificially colonized by blackfly larvae, is proposed for larvicide evaluations. Mortality was recorded 3 or 4 hours after treatment. Larval susceptibility was also evaluated utilizing the LT50 criterion. In there field assays Simulium (C.) pertinax populations from the litoral of S. Paulo and Rio de Janeiro States were shown to be resistant to temephos, even when subjected to high concentrations. Vectobac 12 AS, a Bacillus thuringiensis var. israelensis product, was shown to be more potent against late instar larvae and efficient in concentrations higher than 7,200 ITU/1 (10 min). The LT50 to 3,744 ITU/1 (10 min) was calculated as 70.9 min.O uso de pequenas calhas de madeira no leito de riachos, manualmente colonizadas com borrachudos, é proposto para avaliações da eficiência de larvicidas 3 ou 4 horas após as aplicações. A susceptibilidade larval também foi avaliada pelo critério de TL50. Em três ensaios no litoral do Estado de São Paulo e Rio de Janeiro, populações de Simulium (C.) pertinax Kollar, 1832 mostraram-se resistentes ao temephos, mesmo em altas concentrações. Vectobac 12 AS, à base de Bacillus thuringiensis var. israelensis mostrou-se mais potente contra larvas dos últimos estádios e eficiente para controle em concentrações a partir de 7.200 UIP/1 (10 min.). O TL50para 3.744 UIP/1 (10 min.) foi de 70,9 minutos.36737

Insecticidal Activity of [Cu(H2NTA)2].2H2O in Aedes aegypti Larvae (Diptera: Culicidae)

The demand for new insecticides and alternative strategies for the population control of Aedes aegypti has stimulated research to obtain new compounds with broad biological activity. Accordingly, the dihydrogen nitrilotriacetate complex of copper (II) dihydrate, [Cu(H2NTA)2].2H2O, was synthesized by the stoichiometric reaction of nitrilotriacetic acid (H3NTA) hydroxide with basic copper carbonate (II) (Cu2(OH)2CO3) and characterized by the spectroscopic techniques UV-Vis and FT-IR. The biological toxicity in A. aegypti was determined by bioassay using concentrations ranging from 90.0 mg L−1 to 897.4 mg L−1. The LC50 obtained was 146.11 mg L−1 [132.18–160.10] and the LT50 obtained at a concentration of 897.4 mg L−1 was 70.61 min [38.21–94.90]. The results showed that the complex obtained in this work is a potential metal-insecticide. DOI: http://dx.doi.org/10.17807/orbital.v7i4.73

Magnetic resonance imaging-guided occult breast lesion localization and simultaneous sentinel lymph node mapping

Background: Radio-guided occult lesion localization is a valid technique for the diagnosis of suspicious non-palpable lesions. Here we determine the feasibility of pre-operative localization of occult suspect non-palpable breast lesions using radio-guided occult lesion localization, as well as for identifying the sentinel lymph node.Methods: This is a descriptive study of data collected retrospectively. Pre-operative mapping of 34 breast lesions in 25 patients suspected of being malignant was performed using conventional imaging methods with a magnetic resonance imaging-guided radiopharmaceutical injection.Results: the mean time required to perform the localization was 25 minutes. After resection of the lesions using a gamma probe, malignancy was confirmed in fifteen patients (60.0%), with nine invasive ductal carcinomas, two invasive lobular carcinomas, and four in situ ductal carcinomas the resection was confirmed by the complete removal of the radioactive material. the pathologic results and images were concordant in all but two cases, which were submitted for new magnetic resonance imaging examinations and surgery that confirmed the malignancies. of the 15 patients with confirmed malignancies, 10 had sentinel lymph node resection. of these, eight were negative for metastases, one had micro-metastases and one had confirmed metastases. Three patients had full axillary node dissection, with metastases found in only one. No side effects were observed with magnetic resonance-guided radiopharmaceutical injection.Conclusions: the sentinel node occult lesion localization technique is a simple, reproducible and effective alternative approach to occult lesions compared to other methods, such as mammotomy and the hook-wire localization technique, for mapping suspect breast lesions and identifying lymph node metastasis.Hosp Sirio Libanes, Magnet Resonance Imaging Dept, BR-01308000 São Paulo, BrazilHosp Sirio Libanes, BR-01308000 São Paulo, BrazilHosp Sirio Libanes, Mastol Studies Dept, BR-01308000 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Discipline Mastol, BR-04023062 São Paulo, BrazilHosp Sirio Libanes, Dept Diagnost Imaging, BR-01308000 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Discipline Mastol, BR-04023062 São Paulo, BrazilWeb of Scienc

Insecticidal Activity of [Cu(H2NTA)2].2H2O in Aedes aegypti Larvae (Diptera: Culicidae)

The demand for new insecticides and alternative strategies for the population control of Aedes aegypti has stimulated research to obtain new compounds with broad biological activity. Accordingly, the dihydrogen nitrilotriacetate complex of copper (II) dihydrate, [Cu(H2NTA)2].2H2O, was synthesized by the stoichiometric reaction of nitrilotriacetic acid (H3NTA) hydroxide with basic copper carbonate (II) (Cu2(OH)2CO3) and characterized by the spectroscopic techniques UV-Vis and FT-IR. The biological toxicity in A. aegypti was determined by bioassay using concentrations ranging from 90.0 mg L−1 to 897.4 mg L−1. The LC50 obtained was 146.11 mg L−1 [132.18–160.10] and the LT50 obtained at a concentration of 897.4 mg L−1 was 70.61 min [38.21–94.90]. The results showed that the complex obtained in this work is a potential metal-insecticide. DOI: http://dx.doi.org/10.17807/orbital.v7i4.73

Draft genome sequence of FT9, a novel Bacillus cereus strain isolated from a Brazilian Thermal Spring

O presente trabalho trata das bactérias formadoras da esporos, incluindo um patógeno presente na intoxicação alimentar e sistêmica e em infecções locais, trazendo uma sequência do genoma FT9

COMPARAÇÃO MICROBIOLÓGICA ENTRE MEMBRANAS AMNIÓTICAS HUMANAS COLETADAS EM PARTOS VAGINAIS E CESARIANAS – PROJETO PILOTO

Objective: The amniotic membrane, a thin membrane, may be used as a temporary cover on deep burns. The objective of this study is to identify the differences regarding bacterial contamination between membranes of vaginal and cesarean deliveries, as well as to assess the possibility of the clinical use of stored membranes.Methods: Twelve membranes were obtained from women submitted to vaginal and cesarean deliveries at the Obstetric Center of Hospital de Clínicas de Porto Alegre. Each amniotic membrane was stored in five different flasks containing a physiological solution. Samples were obtained from these flasks for analysis on days 1, 7, 14, 21 and 28. These samples were tested for bacterial contamination, analyzing its relation to time of storage and type of delivery. This is a pilot study with a transversal design.Results: The comparison between types of delivery yielded a relative risk of contamination in vaginal delivery (RR) of 2.67 (95% CI: 1.09-6.52) and no significance (P = 0.08). No contamination was found on day 1 flasks.Conclusion: All membranes derived from vaginal deliveries ended up showing bacterial contamination during the storage period, which lead to a theoretical unavailability for its use on Amniotic Membrane Banks.Objetivo: A membrana amniótica, uma membrana fina, pode ser utilizada como cobertura temporária em queimaduras profundas. O objetivo deste estudo é o de verificar as possíveis diferenças quanto à contaminação bacteriana entre as membranas de partos cesáreo e vaginal, assim como avaliar a viabilidade ou não do uso clínico-cirúrgico da membrana armazenada.Métodos: Foram coletadas 12 membranas amnióticas de mulheres submetidas a parto cesáreo e vaginal no Centro Obstétrico do HCPA. Cada membrana amniótica foi armazenada em cinco frascos diferentes contendo soro fisiológico, dos quais foram obtidas amostras para análise no momento da coleta e nos dias 7, 14, 21 e 28. Essas amostras foram testadas quanto à contaminação bacteriana, analisando sua relação com o tempo de armazenamento e com o tipo de parto realizado. O estudo é um piloto e tem um delineamento transversal. Resultados: A comparação entre os tipos de parto mostrou um risco relativo (RR) de 2,67 de contaminação no parto vaginal em relação à cesariana (IC de 95%: 1,09 a 6,52), P = 0,08. Não foi verificada contaminação em nenhum dos frascos no momento da coleta. Conclusão: Todas as membranas coletadas de parto vaginal apresentaram crescimento bacteriano no processo de estocagem, levando à sua inviabilidade teórica para uso em Bancos de Membrana Amniótica