ลักษณะทางพันธุกรรมระดับเซวล์และชีวโมเลกุลและการเพาะเลี้ยงเนื้อเยื่อของชนิดบานชื่น

Thesis (Ph.D., Biology)--Prince of Songkla University, 200

Influence of medium formula and silver nitrate on in vitro plant regeneration of Zinnia cultivars

The effects of culture medium formula and silver nitrate (AgNO3) on plant regeneration were investigated in vitro using shoot tips of three zinnia cultivars namely Zinnia angustifolia cv. Starbright, Z. haageana cv. Percient Carpet and Z. elegans cv. Dreamland. Shoot organogenesis of all cultivars cultured on KS (Kohlenbach and Schmidt, 1975) medium was found superior to FK (Fukuda and Komamine, 1980), MS (Murashige and Skoog, 1962) and ½MS. Callus formation was found best on MS medium containing 0.1 µM TDZ. The addition of 2 mg/l AgNO3 to KS medium resulted in improvement of the regeneration frequency in Zinnia angustifolia cv. Starbright but not in Z. haageana cv. Percient Carpet and Z. elegans cv. Dreamland. In contrast, callus formation was reduced in all three zinnias. These results indicate that the response to AgNO3 was genotype dependent

Tropane and Granatane Alkaloid Biosynthesis: A Systematic Analysis

The tropane and granatane alkaloids belong to the larger pyrroline and piperidine classes of plant alkaloids, respectively. Their core structures share common moieties and their scattered distribution among angiosperms suggest that their biosynthesis may share common ancestry in some orders, while they may be independently derived in others. Tropane and granatane alkaloid diversity arises from the myriad modifications occurring to their core ring structures. Throughout much of human history, humans have cultivated tropane- and granatane-producing plants for their medicinal properties. This manuscript will discuss the diversity of their biological and ecological roles as well as what is known about the structural genes and enzymes responsible for their biosynthesis. In addition, modern approaches to producing some pharmaceutically important tropanes via metabolic engineering endeavors are discussed