Variabilidade na dormência relativa dos diásporos de Lithraea molleoides (Vell.) Eng.

http://dx.doi.org/10.5902/1980509814570The large diversity of Anacardiaceae diaspores morphology has a notable repercussion in the processes of germination and emergence besides generating a differentiated behavior among the species. In this family, there are some species that present physical dormancy, some others present mechanical dormancy, others present no dormancy at all and some of them have no information detailed in the available literature. Lithraea molleoides (Vell.) Eng. is one of the species which little is known regarding to dormancy. Therefore, the objective was to evaluate the germination and the emergence processes of seedlings from diaspores of this species collected in Araguari river valley, in the state of Minas Gerais, as well as to quantify the variability of dormancy intensity present among different individuals of the population. The diaspores were collected in October and November of 2004 and 2005, from six matrixes. Two experiments were set up. The germination experiment was conducted under controlled conditions and the emergence experiment was conducted in a semi-open greenhouse. Data was collected every 24 hours, up serving the protrusion of the embryo in the experiment setup in the laboratory, and the emergence of the hypocotyl of the seedling above the substrate in the experiment kept in the greenhouse. In both experiments, the diaspores originated from plant number two were more notable to some extent, having superior physiological quality compared to the others (average time between 4 and 22 days; average speeds between 0.042 and 0.0217 days-1; uncertainty between 1.62 and 3.5 bits and synchrony between 0.04 and 0.381). The high uncertainty values and the low synchrony values indicate that the germination and emergence processes of the Lithraea molleoides seedlings are spread in time, which allows us to characterize them as bearers of relative dormancy, although its intensity is variable among individuals of the same population. Low probabilities, however not null, to germinate or emerge even after 150 and 60 days after sowing, respectively, showing the temporal survival capacity of the diaspores of the species and reinforce the presence of relative dormancy.http://dx.doi.org/10.5902/1980509814570A grande diversidade na morfologia dos diásporos da família Anacardiaceae repercute nos processos de germinação e emergência e gera comportamento diferenciado entre as espécies. Dentro dessa família há algumas espécies que apresentam dormência física, outras mecânica, algumas que não exibem dormência e há também espécies cuja informação não está explícita na literatura disponível. Lithraea molleoides (Vell.) Eng. é uma das espécies que hoje se encontra em posição duvidosa quanto à dormência. Em razão disso, objetivou-se avaliar o processo de germinação e emergência de plântulas provenientes de diásporos da espécie, coletados no Vale do Rio Araguari, MG, bem como quantificar a variabilidade na intensidade da dormência existente entre diferentes indivíduos da população. Os diásporos foram coletados em outubro e novembro de 2004 e 2005, a partir de seis matrizes, sendo instalados dois experimentos, o de germinação, montado sob condições controladas, e outro de emergência, em estufa semiaberta. A coleta dos dados foi realizada a cada 24 horas, observando-se a protrusão do embrião no experimento montado em laboratório e a emergência do hipocótilo da plântula acima do substrato no experimento mantido em estufa. Em ambos os experimentos, os diásporos oriundos da matriz de número dois se destacaram em alguma medida, tendo qualidade fisiológica superior em relação aos demais (tempo médio entre 4 e 22 dias; velocidade média entre 0,042 e 0,0217 dias-1; incerteza entre 1,62 e 3,5 bits e sincronia entre 0,04 e 0,381). Os altos valores de incerteza e os baixos valores de sincronia indicam que os processos de germinação e emergência de plântulas de L. molleoides são espalhados no tempo, o que permite caracterizá-los como portadores de dormência relativa, embora sua intensidade seja variável entre indivíduos de uma mesma população. Probabilidades baixas, porém, não nulas para germinar ou emergir, mesmo aos 150 e 60 dias após a semeadura, respectivamente, revelam a capacidade de sobrevivência temporal dos diásporos da espécie e reforçam a presença de dormência relativa

VARIABILITY IN THEIR RELATIVE DORMANCY OF Lithraea molleoides (Vell.) Eng. DIASPORES

A grande diversidade na morfologia dos di\ue1sporos da fam\uedlia Anacardiaceae repercute nos processos de germina\ue7\ue3o e emerg\ueancia e gera comportamento diferenciado entre as esp\ue9cies. Dentro dessa fam\uedlia h\ue1 algumas esp\ue9cies que apresentam dorm\ueancia f\uedsica, outras mec\ue2nica, algumas que n\ue3o exibem dorm\ueancia e h\ue1 tamb\ue9m esp\ue9cies cuja informa\ue7\ue3o n\ue3o est\ue1 expl\uedcita na literatura dispon\uedvel. Lithraea molleoides (Vell.) Eng. \ue9 uma das esp\ue9cies que hoje se encontra em posi\ue7\ue3o duvidosa quanto \ue0 dorm\ueancia. Em raz\ue3o disso, objetivou-se avaliar o processo de germina\ue7\ue3o e emerg\ueancia de pl\ue2ntulas provenientes de di\ue1sporos da esp\ue9cie, coletados no Vale do Rio Araguari, MG, bem como quantificar a variabilidade na intensidade da dorm\ueancia existente entre diferentes indiv\uedduos da popula\ue7\ue3o. Os di\ue1sporos foram coletados em outubro e novembro de 2004 e 2005, a partir de seis matrizes, sendo instalados dois experimentos, o de germina\ue7\ue3o, montado sob condi\ue7\uf5es controladas, e outro de emerg\ueancia, em estufa semiaberta. A coleta dos dados foi realizada a cada 24 horas, observando-se a protrus\ue3o do embri\ue3o no experimento montado em laborat\uf3rio e a emerg\ueancia do hipoc\uf3tilo da pl\ue2ntula acima do substrato no experimento mantido em estufa. Em ambos os experimentos, os di\ue1sporos oriundos da matriz de n\ufamero dois se destacaram em alguma medida, tendo qualidade fisiol\uf3gica superior em rela\ue7\ue3o aos demais (tempo m\ue9dio entre 4 e 22 dias; velocidade m\ue9dia entre 0,042 e 0,0217 dias-1; incerteza entre 1,62 e 3,5 bits e sincronia entre 0,04 e 0,381). Os altos valores de incerteza e os baixos valores de sincronia indicam que os processos de germina\ue7\ue3o e emerg\ueancia de pl\ue2ntulas de L. molleoides s\ue3o espalhados no tempo, o que permite caracteriz\ue1-los como portadores de dorm\ueancia relativa, embora sua intensidade seja vari\ue1vel entre indiv\uedduos de uma mesma popula\ue7\ue3o. Probabilidades baixas, por\ue9m, n\ue3o nulas para germinar ou emergir, mesmo aos 150 e 60 dias ap\uf3s a semeadura, respectivamente, revelam a capacidade de sobreviv\ueancia temporal dos di\ue1sporos da esp\ue9cie e refor\ue7am a presen\ue7a de dorm\ueancia relativa.The large diversity of Anacardiaceae diaspores morphology has a notable repercussion in the processes of germination and emergence besides generating a differentiated behavior among the species. In this family, there are some species that present physical dormancy, some others present mechanical dormancy, others present no dormancy at all and some of them have no information detailed in the available literature. Lithraea molleoides (Vell.) Eng. is one of the species which little is known regarding to dormancy. Therefore, the objective was to evaluate the germination and the emergence processes of seedlings from diaspores of this species collected in Araguari river valley, in the state of Minas Gerais, as well as to quantify the variability of dormancy intensity present among different individuals of the population. The diaspores were collected in October and November of 2004 and 2005, from six matrixes. Two experiments were set up. The germination experiment was conducted under controlled conditions and the emergence experiment was conducted in a semi-open greenhouse. Data was collected every 24 hours, up serving the protrusion of the embryo in the experiment setup in the laboratory, and the emergence of the hypocotyl of the seedling above the substrate in the experiment kept in the greenhouse. In both experiments, the diaspores originated from plant number two were more notable to some extent, having superior physiological quality compared to the others (average time between 4 and 22 days; average speeds between 0.042 and 0.0217 days-1; uncertainty between 1.62 and 3.5 bits and synchrony between 0.04 and 0.381). The high uncertainty values and the low synchrony values indicate that the germination and emergence processes of the Lithraea molleoides seedlings are spread in time, which allows us to characterize them as bearers of relative dormancy, although its intensity is variable among individuals of the same population. Low probabilities, however not null, to germinate or emerge even after 150 and 60 days after sowing, respectively, showing the temporal survival capacity of the diaspores of the species and reinforce the presence of relative dormancy

Mobilização de reservas no eixo embrionário e nos cotilédones de sementes de Schizolobium parahyba (vell.) S. F. Blake durante a germinação

O conhecimento da composição química da semente é de extrema importância para auxiliar o entendimento do comportamento durante a germinação, o armazenamento ou mesmo nos processos metabólicos envolvidos na degradação dessas reservas, que, em última instância, afetam o vigor das sementes. Dessa forma, o objetivo deste trabalho foi estudar as alterações no conteúdo de carboidratos, lipídios e proteínas nos cotilédones e no eixo embrionário, durante a germinação de sementes de Schizolobium parahyba (Vell.) S. F. Blake. Para tanto, as sementes foram colocadas para germinar e retiradas amostras periodicamente para as análises. A porcentagem de germinação foi de 88%, iniciando-se no 9o dia, e a velocidade de germinação foi de 7,7 sementes.dia-1. Dentre os ácidos graxos, o ácido oléico predominou tanto nos cotilédones, quanto no eixo embrionário, com variações durante o período de observação. Os demais tiveram pequena variação e não diferiram de forma expressiva. Os teores de amido e de carboidratos solúveis totais comportaram-se de forma similar nos dois compartimentos com decréscimo ao longo do período de embebição. O teor de proteínas no eixo embrionário, após aumentar em dois dias, decresceu até o 8o dia. Por outro lado, o teor nos cotilédones manteve-se constante nos dois primeiros dias, vindo a cair até o 6o, com aumento no 8o.Knowledge of seed chemical composition is very important in order to understand behavior during germination, storage and metabolic processes involved during reserve degradation, the latter of which affects seed vigor. The objective of this study was to assess changes in carbohydrates, lipids and proteins in the embryonic axis and in cotyledons during germination of Schizolobium parahyba. Seeds were placed in Petri dishes to germinate and samples were removed periodically for analyses. Germination was 88%, starting on the ninth day, and the germination rate was 7.7 seeds.day-1. Among fatty acids, oleic acids predominated in the embryonic axis and in cotyledons, with changes occurring during the observation period. The others fatty acids showed little change. The levels of starch and soluble carbohydrate behaved in a similar manner in both compartments with a decrease during the imbibition period. Protein levels in the embryonic axis decreased by the eight day, after increasing for two days. On the other hand, protein levels in cotyledons remained constant during the first two days, decreased on the six day and increased on the eight day

Seed reserve mobilization in the embryonic axis and cotyledons of Schizolobium parahyba (Vell.) S. F. Blake during germination

O conhecimento da composi\ue7\ue3o qu\uedmica da semente \ue9 de extrema import\ue2ncia para auxiliar o entendimento do comportamento durante a germina\ue7\ue3o, o armazenamento ou mesmo nos processos metab\uf3licos envolvidos na degrada\ue7\ue3o dessas reservas, que, em \ufaltima inst\ue2ncia, afetam o vigor das sementes. Dessa forma, o objetivo deste trabalho foi estudar as altera\ue7\uf5es no conte\ufado de carboidratos, lip\ueddios e prote\uednas nos cotil\ue9dones e no eixo embrion\ue1rio, durante a germina\ue7\ue3o de sementes de Schizolobium parahyba (Vell.) S. F. Blake. Para tanto, as sementes foram colocadas para germinar e retiradas amostras periodicamente para as an\ue1lises. A porcentagem de germina\ue7\ue3o foi de 88%, iniciando-se no 9o dia, e a velocidade de germina\ue7\ue3o foi de 7,7 sementes.dia-1. Dentre os \ue1cidos graxos, o \ue1cido ol\ue9ico predominou tanto nos cotil\ue9dones, quanto no eixo embrion\ue1rio, com varia\ue7\uf5es durante o per\uedodo de observa\ue7\ue3o. Os demais tiveram pequena varia\ue7\ue3o e n\ue3o diferiram de forma expressiva. Os teores de amido e de carboidratos sol\ufaveis totais comportaram-se de forma similar nos dois compartimentos com decr\ue9scimo ao longo do per\uedodo de embebi\ue7\ue3o. O teor de prote\uednas no eixo embrion\ue1rio, ap\uf3s aumentar em dois dias, decresceu at\ue9 o 8o dia. Por outro lado, o teor nos cotil\ue9dones manteve-se constante nos dois primeiros dias, vindo a cair at\ue9 o 6o, com aumento no 8o.Knowledge of seed chemical composition is very important in order to understand behavior during germination, storage and metabolic processes involved during reserve degradation, the latter of which affects seed vigor. The objective of this study was to assess changes in carbohydrates, lipids and proteins in the embryonic axis and in cotyledons during germination of Schizolobium parahyba . Seeds were placed in Petri dishes to germinate and samples were removed periodically for analyses. Germination was 88%, starting on the ninth day, and the germination rate was 7.7 seeds.day-1. Among fatty acids, oleic acids predominated in the embryonic axis and in cotyledons, with changes occurring during the observation period. The others fatty acids showed little change. The levels of starch and soluble carbohydrate behaved in a similar manner in both compartments with a decrease during the imbibition period. Protein levels in the embryonic axis decreased by the eight day, after increasing for two days. On the other hand, protein levels in cotyledons remained constant during the first two days, decreased on the six day and increased on the eight day

Intra-specific variability in Lithraea molleoides (Vell.) Eng. from germination and emergence processes

The great diversity of morphology in diaspores of Anacardiaceae reflects in germination and dormancy processes and generates a differentiated behavior among species. There are some species within this family that show physical dormancy, others show mechanical dormancy, some that do not show dormancy and also species that lack this information in the available literature. Lithraea molleoides is among the species whose dormancy has not yet been elucidated. The objective of this work was therefore to study germination and emergence of seedlings originated from diaspores recently collected from different donor plants occurring in the Rio Araguari Valley, MG, as well as behavior of diaspores after storage in cold chamber. Diaspores were collected from eight donor plants in October and November, 2004 and 2005. Six experiments were carried out, being two with recently-collected diaspores, two with diaspores stored for six months in cold chamber and two with diaspores stored for 12 months in the same condition. The experiments were conducted under controlled laboratory conditions and uncontrolled conditions of a semi-open greenhouse. Data collection was carried out every 24 h, by recording embryo protrusion (botanical criteria) in the laboratory experiments and seedling hypocotyl emergence above the substrate in the greenhouse experiments. In all the experiments, the diaspores derived from donor plant number 2 stood out in some way, showing better physiological quality compared with the others (average time between 4 and 22 days, average speed between 0.042 and 0.217 day-1, uncertainty between 1.62 and 3.53 bits and synchrony between 0.040 and 0.381). The large values of average time and uncertainty, as well as low synchrony, indicate that the processes of germination and seedling emergence of Lithraea molleoides, independently of diaspore age, are dispersed in time, characterizing them as exhibiting relative dormancy and being resistant to dry storage. It is worth emphasizing that the study of the sample population of this species was representative, since the collected donor plants showed similar behavior in all experiments. These characteristics are important for the species conservation.Mestre em AgronomiaA grande diversidade na morfologia dos diásporos da família Anacardiaceae repercute nos processos de germinação e dormência e gera um comportamento diferenciado entre as espécies. Dentro dessa família há algumas espécies que apresentam dormência física, outras mecânica, algumas que não exibem dormência e há também espécies cuja informação não está explícita na literatura disponível. Lithraea molleoides é uma das espécies que hoje se encontram em posição duvidosa quanto à dormência. Em razão disso, este trabalho teve por objetivos estudar a germinação e a emergência de plântulas provenientes de diásporos recém-coletados de diferentes matrizes ocorrentes no Vale do Rio Araguari, MG, bem como o comportamento desses diásporos após o armazenamento em câmara fria. Os diásporos foram coletados em outubro e novembro de 2004 e 2005, a partir de oito matrizes. Foram instalados seis experimentos, sendo dois com os diásporos recém-coletados, dois com os diásporos armazenados por seis meses em câmara fria e dois com diásporos armazenados por 12 meses na mesma condição. Esses experimentos foram montados em condições controladas de laboratório e em condições não-controladas de estufa semi-aberta. A coleta dos dados foi realizada a cada 24 horas, observando-se a protrusão do embrião (critério botânico) nos experimentos mantidos em laboratório e a emergência do hipocótilo da plântula acima do substrato nos experimentos mantidos em estufa. Em todos os experimentos, os diásporos oriundos da matriz de número dois se destacaram em alguma medida, tendo qualidade fisiológica superior em relação aos demais (tempo médio entre 4 e 22 dias; velocidade média entre 0,042 e 0,217 dias-1, incerteza entre 1,62 e 3,53 bits e sincronia entre 0,040 e 0,381). Os altos valores do tempo médio e da incerteza, bem como os baixos valores de sincronia, indicam que os processos de germinação e emergência de plântulas de Lithraea molleoides, independentemente da idade dos diásporos, são espalhados no tempo, o que permite caracterizá-los como portadores de dormência relativa e resistentes ao armazenamento a seco. Vale destacar ainda que o estudo da amostra da população dessa espécie foi representativo, uma vez que as matrizes coletadas apresentaram comportamento similar em todos os experimentos montados. Tais características são importantes para a conservação da espécie

Mobilização de reservas no eixo embrionário e nos cotilédones de sementes de <i>Schizolobium parahyba</i> (Vell.) S. F. Blake durante a germinação

Knowledge of seed chemical composition is very important in order to understand behavior during germination, storage and metabolic processes involved during reserve degradation, the latter of which affects seed vigor. The objective of this study was to assess changes in carbohydrates, lipids and proteins in the embryonic axis and in cotyledons during germination of Schizolobium parahyba. Seeds were placed in Petri dishes to germinate and samples were removed periodically for analyses. Germination was 88%, starting on the ninth day, and the germination rate was 7.7 seeds.day-1. Among fatty acids, oleic acids predominated in the embryonic axis and in cotyledons, with changes occurring during the observation period. The others fatty acids showed little change. The levels of starch and soluble carbohydrate behaved in a similar manner in both compartments with a decrease during the imbibition period. Protein levels in the embryonic axis decreased by the eight day, after increasing for two days. On the other hand, protein levels in cotyledons remained constant during the first two days, decreased on the six day and increased on the eight day.</p

4Th Pediatric Allergy And Asthma Meeting (Paam)

WORKSHOP 4: Challenging clinical scenarios (CS01–CS06), CS01 Bullous lesions in two children: solitary mastocytoma, S. Tolga Yavuz, Ozan Koc, Ali Gungor, Faysal Gok, CS02 Multi-System Allergy (MSA) of cystic fibrosis: our institutional experience, Jessica Hawley, Christopher O’Brien, Matthew Thomas, Malcolm Brodlie, Louise Michaelis, CS03 Cold urticaria in pediatric age: an invisible cause for severe reactions, Inês Mota, Ângela Gaspar, Susana Piedade, Graça Sampaio, José Geraldo Dias, Miguel Paiva, Mário Morais-Almeida, CS04 Angioedema with C1 inhibitor deficiency in a girl: a challenge diagnosis, Cristina Madureira, Tânia Lopes, Susana Lopes, Filipa Almeida, Alexandra Sequeira, Fernanda Carvalho, José Oliveira, CS05 A child with unusual multiple organ allergy disease: what is the primer?, Fabienne Gay-Crosier, CS06 A case of uncontrolled asthma in a 6-year-old patient, Ioana-Valentina Nenciu, Andreia Florina Nita, Alexandru Ulmeanu, Dumitru Oraseanu, Carmen Zapucioiu, ORAL ABSTRACT SESSION 1: Food allergy (OP01–OP06), OP01 Food protein-induced enterocolitis syndrome: oral food challenge outcomes for tolerance evaluation in a Pediatric Hospital, Adrianna Machinena, Olga Domínguez Sánchez, Montserrat Alvaro Lozano, Rosa Jimenez Feijoo, Jaime Lozano Blasco, Mònica Piquer Gibert, Mª Teresa Giner Muñoz, Marcia Dias da Costa, Ana Maria Plaza Martín, OP02 Characteristics of infants with food protein-induced enterocolitis syndrome and allergic proctocolitis, Ebru Arik Yilmaz, Özlem Cavkaytar, Betul Buyuktiryaki, Ozge Soyer, Cansin Sackesen, OP03 The clinical and immunological outcomes after consumption of baked egg by 1–5 year old egg allergic children: results of a randomised controlled trial, MerrynNetting, Adaweyah El-Merhibi, Michael Gold, PatrickQuinn, IrmeliPenttila, Maria Makrides, OP04 Oral immunotherapy for treatment of egg allergy using low allergenic, hydrolysed egg, Stavroula Giavi, Antonella Muraro, Roger Lauener, Annick Mercenier, Eugen Bersuch, Isabella M. Montagner, Maria Passioti, Nicolò Celegato, Selina Summermatter, Sophie Nutten, Tristan Bourdeau, Yvonne M. Vissers, Nikolaos G. Papadopoulos, OP05 Chemical modification of a peanut extract results in an increased safety profile while maintaining efficacy, Hanneke van der Kleij, Hans Warmenhoven, Ronald van Ree, Raymond Pieters, Dirk Jan Opstelten, Hans van Schijndel, Joost Smit, OP06 Administration of the yellow fever vaccine in egg allergic children, Roisin Fitzsimons, Victoria Timms, George Du Toit, ORAL ABSTRACT SESSION 2: Asthma (OP07–OP12), OP07 Previous exacerbation is the most important risk factor for future exacerbations in school-age children with asthma, S. Tolga Yavuz, Guven Kaya, Mustafa Gulec, Mehmet Saldir, Osman Sener, Faysal Gok, OP08 Comparative study of degree of severity and laboratory changes between asthmatic children using different acupuncture modalities, Nagwa Hassan, Hala Shaaban, Hazem El-Hariri, Ahmed Kamel Inas E. Mahfouz, OP09 The concentration of exhaled carbon monoxide in asthmatic children with different controlled stadium, Papp Gabor, Biro Gabor, Kovacs Csaba, OP10 Effect of vitamin D3 supplementation during pregnancy on risk of persistent wheeze in the offspring: a randomised clinical trial, Bo Chawes, Klaus Bønnelykke, Jakob Stokholm, Lene Heickendorff, Susanne Brix, Morten Rasmussen, Hans Bisgaard, OP11 Lung function development in childhood, Henrik Wegener Hallas, Bo Chawes, Lambang Arianto, Hans Bisgaard, OP12 Is the effect of maternal and paternal asthma different in female and male children before puberty?, Maike Pincus, Thomas Keil, Andreas Reich, Ulrich Wahn, Susanne Lau, Linus Grabenhenrich, ORAL ABSTRACT SESSION 3: Epidemiology—genetics (OP13–OP18), OP13 Lifestyle is associated with incidence and category of allergen sensitisation: the ALADDIN birth cohort, Sara Fagerstedt, Helena Marell Hesla, Emelie Johansson, Helen Rosenlund, Axel Mie, Annika Scheynius, Johan Alm, OP15 Maternal filaggrin mutations increase the risk of atopic dermatitis in children: an effect independent of mutation inheritance, Jorge Esparza-Gordillo, Anja Matanovic, Ingo Marenholz, Anja Bauerfeind, Klaus Rohde, Katja Nemat, Min-Ae Lee-Kirsch, Magnus Nordenskjöld, Marten C. G. Winge, Thomas Keil, Renate Krüger, Susanne Lau, Kirsten Beyer, Birgit Kalb, Bodo Niggemann, Norbert Hübner, Heather J. Cordell, Maria Bradley, Young-Ae Lee, OP16 Allergic multimorbidity of asthma, rhinitis and eczema in the first 2 decades of the German MAS birth cohort, Thomas Keil, Hannah Gough, Linus Grabenhenrich, Dirk Schramm, Andreas Reich, John Beschorner, Antje Schuster, Carl-Peter Bauer, Johannes Forster, Fred Zepp, Young-Ae Lee, Renate Bergmann, Karl Bergmann, Ulrich Wahn, Susanne Lau, OP17 Childhood anaphylaxis: a growing concern, Filipe Benito Garcia, Inês Mota, Susana Piedade, Ângela Gaspar, Natacha Santos, Helena Pité, Mário Morais-Almeida, OP18 Indoor exposure to molds and dampness in infancy and its association to persistent atopic dermatitis in school age. Results from the Greek ISAAC II study, Athina Papadopoulou, Despina Mermiri, Elpida Xatziagorou, Ioannis Tsanakas, Stavroula Lampidi, Kostas Priftis, ORAL ABSTRACT SESSION 4: Pediatric rhinitis—immunotherapy (OP19–OP24), OP19 Associations between residential greenness and childhood allergic rhinitis and aeroallergen sensitisation in seven birth cohorts, Elaine Fuertes, Iana Markevych, Gayan Bowatte, Olena Gruzieva, Ulrike Gehring, Allan Becker, Dietrich Berdel, Michael Brauer, Chris Carlsten, Barbara Hoffmann, Anita Kozyrskyj, Caroline Lodge, Göran Pershagen, Alet Wijga, Heinrich Joachim, OP20 Full symptom control in pediatric patients with allergic rhinitis and asthma: results of a 2-year sublingual allergen immunotherapy study, Zorica Zivkovic, Ivana Djuric-Filipovic, Jasmina Jocić-Stevanovic, Snežana Zivanovic, OP21 Nasal epithelium of different ages of atopic subjects present increased levels of oxidative stress and increased cell cytotoxicity upon rhinovirus infection, Styliani Taka, Dimitra Kokkinou, Aliki Papakonstantinou, Panagiota Stefanopoulou, Anastasia Georgountzou, Paraskevi Maggina, Sofia Stamataki, Vassiliki Papaevanggelou, Evangelos Andreakos, Nikolaos G. Papadopoulos, OP22 Cluster subcutaneous immunotherapy schedule: tolerability profile in children, Monica Piquer Gibert, Montserrat Alvaro Lozano, Jaime Lozano Blasco, Olga Domínguez Sánchez, Rosa Jiménez Feijoo, Marcia Dias da Costa, Mª Teresa Giner Muñoz, Adriana Machinena Spera, Ana Maria Plaza Martín, OP23 Rhinitis as a risk factor for asthma severity in 11-year old children: population-based cohort study, Matea Deliu, Danielle Belgrave, Angela Simpson, Adnan Custovic, OP24 The Global Lung Function Initiative equations in airway obstruction evaluation of asthmatic children, João Gaspar Marques, Pedro Carreiro-Martins, Joana Belo, Sara Serranho, Isabel Peralta, Nuno Neuparth, Paula Leiria-Pinto, POSTER DISCUSSION SESSION 1: Food allergy (PD01–PD05), PD01 Allergen-specific humoral and cellular responses in children who fail egg oral immunotherapy due to allergic reactions, Marta Vazquez-Ortiz, Mariona Pascal, Ana Maria Plaza, Manel Juan, PD02 FoxP3 epigenetic features in children with cow milk allergy, Lorella Paparo, Rita Nocerino, Rosita Aitoro, Ilaria Langella, Antonio Amoroso, Alessia Amoroso, Carmen Di Scala, Roberto Berni Canani, PD04 Combined milk and egg allergy in early childhood: let them eat cake?, Santanu Maity, Giuseppina Rotiroti, Minal Gandhi, PD05 Introduction of complementary foods in relation to allergy and gut microbiota in farm and non-farm children, Karin Jonsson, Annika Ljung, Bill Hesselmar, Ingegerd Adlerbert, Hilde Brekke, Susanne Johansen, Agnes Wold, Ann-Sofie Sandberg, POSTER DISCUSSION SESSION 2: Asthma and wheeze (PD06–PD16), PD06 The association between asthma and exhaled nitric oxide is influenced by genetics and sensitisation, Björn Nordlund, Cecilia Lundholm, Villhelmina Ullemar, Marianne van Hage, Anne Örtqvist, Catarina Almqvist, PD09 Prevalence patterns of infant wheeze across Europe, Anna Selby, Kate Grimshaw, Thomas Keil, Linus Grabenhenrich, Michael Clausen, Ruta Dubakiene, Alessandro Fiocchi, Marek Kowalski, Nikos Papadopoulos, Marta Reche, Sigurveig Sigurdardottir, Aline Sprikkleman, Paraskevi Xepapadaki, Clare Mills, Kirsten Beyer, Graham Roberts, PD10 Epidemiologic changes in recurrent wheezing infants, Herberto Jose Chong Neto, Gustavo Falbo Wandalsen, Ana Carolina Dela Bianca, Carolina Aranda, Nelson Augusto Rosário, Dirceu Solé, Javier Mallol, Luis García Marcos, PD13 A single nucleotide polymorphism in the GLCCI1 gene is associated with response to asthma treatment in children, IvanaBanic, Matija Rijavec, Davor Plavec, Peter Korosec, Mirjana Turkalj, PD14 Pollen induced asthma: Could small molecules in pollen exacerbate the protein-mediated allergic response?, Alen Bozicevic, Maria De Mieri, Matthias Hamburger, PD15 A qualitative study to understand how we can empower teenagers to better self-manage their asthma, Simone Holley, Ruth Morris, Frances Mitchell, Rebecca Knibb, Susan Latter, Christina Liossi, Graham Roberts, PD16 Polymorphism of endothelial nitric oxide synthase (eNOS) gene among Egyptian children with bronchial asthma, Mostafa M. M. Hassan, POSTER DISCUSSION SESSION 3: Mechanisms—Epidemiology (PD17–PD21), PD17 Pregnancy outcomes in relation to development of allergy in a Swedish birth cohort, Malin Barman, Anna Sandin, Agnes Wold, Ann-Sofie Sandberg, PD18 Evolution of the IgE response to house dust mite molecules in childhood, Daniela Posa, Serena Perna, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, Ulrich Wahn, Thomas Keil, Susanne Lau, Kuan-Wei Chen, Yvonne Resch, Susanne Vrtala, Rudolf Valenta, Paolo Maria Matricardi, PD19 Antibody recognition of nsLTP-molecules as antigens but not as allergens in the German-MAS birth cohort, Olympia Tsilochristou, Alexander Rohrbach, Antonio Cappella, Stephanie Hofmaier, Laura Hatzler, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, RaffaeleD’Amelio, Ulrich Wahn, Thomas Keil, Susanne Lau, Paolo Maria Matricardi, PD20 Early life colonization with Lactobacilli and Staphylococcus aureus oppositely associates with the maturation and activation of FOXP3+ CD4 T-cells, Sophia Björkander, Maria A. Johansson, Gintare Lasaviciute, Eva Sverremark-Ekström, PD21 Genome-wide meta-analysis identifies 7 susceptibility loci involved in the atopic march, Ingo Marenholz, Jorge Esparza-Gordillo, Franz Rüschendorf, Anja Bauerfeind, David P. Strachan, Ben D. Spycher, Hansjörg Baurecht, Patricia Margaritte-Jeannin, Annika Sääf, Marjan Kerkhof, Markus Ege, Svetlana Baltic, Melanie C Matheson, Jin Li, Sven Michel, Wei Q. Ang, Wendy McArdle, Andreas Arnold, Georg Homuth, Florence Demenais, Emmanuelle Bouzigon, Cilla Söderhäll, Göran Pershagen, Johan C. de Jongste, Dirkje S Postma, Charlotte Braun-Fahrländer, Elisabeth Horak, Ludmila M. Ogorodova, Valery P. Puzyrev, Elena Yu Bragina, Thomas J Hudson, Charles Morin, David L Duffy, Guy B Marks, Colin F Robertson, Grant W Montgomery, Bill Musk, Philip J Thompson, Nicholas G. Martin, Alan James, Patrick Sleiman, Elina Toskala, Elke Rodriguez, Regina Fölster-Holst, Andre Franke, Wolfgang Lieb, Christian Gieger, Andrea Heinzmann, Ernst Rietschel, Thomas Keil, Sven Cichon, Markus M Nöthen, Craig E Pennell, Peter D Sly, Carsten O Schmidt, Anja Matanovic, Valentin Schneider, Matthias Heinig, Norbert Hübner, Patrick G. Holt, Susanne Lau, Michael Kabesch, Stefan Weidinger, Hakon Hakonarson, Manuel AR Ferreira, Catherine Laprise, Maxim B. Freidin, Jon Genuneit, Gerard H Koppelman, Erik Melén, Marie-Hélène Dizier, A. John Henderson, Young Ae Lee, POSTER DISCUSSION SESSION 4: Food allergy—Anaphylaxis (PD22–PD26), PD22 Atopy patch test in food protein induced enterocolitis caused by solid food, Purificacion González-Delgado, Esther Caparrós, Fernando Clemente, Begoña Cueva, Victoria M. Moreno, Jose Luis Carretero, Javier Fernández, PD23 Watermelon allergy: a novel presentation, Kate Swan, George Du Toit, PD24 A pilot study evaluating the usefulness of a guideline template for managing milk allergy in primary care, Mudiyur Gopi, Tim Smith, Edara Ramesh, Arun Sadasivam, PD26 Efficacy and safety of cow’s milk oral immunotherapy protocol, Inês Mota, Filipe Benito Garcia, Susana Piedade, Angela Gaspar, Graça Sampaio, Cristina Arêde, Luís Miguel Borrego, Graça Pires, Cristina Santa-Marta, Mário Morais-Almeida, POSTER DISCUSSION SESSION 5: Prevention and treatment—Allergy (PD27–PD36), PD27 Allergy-protection by the lactic acid bacterium Lactococcus lactis G121: mode-of-action as revealed in a murine model of experimental allergy, Stephanie Brand, Karina Stein, Holger Heine, Marion Kauth, PD29 The relationship between quality of life and morning salivary cortisol after acute bronchiolitis in infancy, Leif Bjarte Rolfsjord, Egil Bakkeheim, Johan Alm, Håvard Ove Skjerven, Kai-Håkon Carlsen, Jon Olav Hunderi, Teresa Løvold Berents, Petter Mowinckel, Karin C. Lødrup Carlsen, PD30 Randomised trial of the efficacy of MP29-02* compared with fluticasone propionate nasal spray in children aged ≥6 years to <12 years with allergic rhinitis, Ulrich Wahn, Ullrich Munzel, William Berger, PD31 10 mg of oral bilastine in 2 to 11 years old children has similar exposure to the adult therapeutic dose (20 mg), Ulrich Wahn, Román Valiente, Valvanera Vozmediano, John C. Lukas, Mónica Rodríguez, PD33 Daily symptoms, nocturnal symptoms, activity limitations and reliever therapies during the three steps of IOEASMA programme: a comparison, Sebastiano Guarnaccia, Luigi Vitale, Ada Pluda, Emanuele D’Agata, Denise Colombo, Stefano Felici, Valeria Gretter, Susanna Facchetti, Gaia Pecorelli, Cristina Quecchia, PD34 Sensitisation to an inert aeroallergen in weaning rats and longstanding disease, in a sensitisation-tolerant and easily tolerisable rodent strain, George Guibas, Evangelia Spandou, Spyridon Megremis, Peter West, Nikolaos Papadopoulos, PD35 Bacterial and fungi exposure in school and allergic sensitisation in children, João Cavaleiro Rufo, Joana Madureira, Inês Paciência, Lívia Aguiar, Patrícia Padrão, Mariana Pinto, Luís Delgado, Pedro Moreira, João Paulo Teixeira, Eduardo Oliveira Fernandes, André Moreira, PD36 Comparative study of allergy rhinitis between two populations: children vs. adults, Adriana Izquierdo Dominguez, Antonio Valero, Joaquim Mullol, Alfonso Del Cuvillo, Javier Montoro, Ignacio Jauregui, Joan Bartra, Ignacio Davila, Marta Ferrer, Joaquin Sastre, POSTER VIEWING SESSION 1: Inflammation—Genetics—Immunology—Dermatology (PP01–PP09), PP01 Immune profile in late pregnancy: immunological markers in atopic asthmaticwomen as risk factors for atopy in the progeny, Catarina Martins, Jorge Lima, Maria José Leandro, Glória Nunes, Jorge Cunha Branco, Hélder Trindade, Luis Miguel Borrego, PP02 The impact of neonatal sepsis on development of allergic diseases, Secil Conkar, Mehtap Kilic, Canan Aygun, Recep Sancak, PP03 Clinical overview of selective IgE deficiency in childhood, Athina Papadopoulou, Eleni Tagalaki, Lambros Banos, Anna Vlachou, Fotini Giannoula, Despina Mermiri, PP04 Inverse relationship between serum 25(ΟΗ) vitamin D3 and total IgE in children and adolescence, Athina Papadopoulou, Stavroula Lampidi, Marina Pavlakou, Maria Kryoni, Kostas Makris, PP05, PP06, PP07 Asthma control questionnaire and specific IgE in children, Snezhina Lazova, Guergana Petrova, Dimitrinka Miteva, Penka Perenovska, PP08 Features of chronic urticaria of adolescents, Aliya Klyucharova, Olesya Skorohodkina, PP09 Cutaneous mastocytosis in children: a clinical analysis of 8 cases in Greece, Dimitra Koumaki, Alkisti Manousaki, Maria Agrapidi, Lida Iatridou, Omima Eruk, Konstantinos Myridakis, Emmanouil Manousakis, Vasiliki Koumaki, POSTER VIEWING SESSION 2: Food allergy—Anaphylaxis (PP10–PP47), PP10 Prognostic factors in egg allergy, Maria Dimou, Maria Ingemansson, Gunilla Hedlin, PP11 Evaluation of the efficacy of an amino acid-based formula in infants who are intolerant to extensively hydrolysed protein formula, Nitida Pastor, Delphine de Boissieu, Jon Vanderhoof, Nancy Moore, Kaitlin Maditz, PP12 Anaphylaxis and epinephrine auto-injector use: a survey of pediatric trainees, Adeli Mehdi, Shaza Elhassan, Carolin Beck, Ahmed Al-Hammadi, PP13 Anaphylaxis in children: acute management in the Emergency Department, Ioana Maris, Ronan O’Sullivan, Jonathan Hourihane,, PP14 Understanding Cumbrian schools preparedness in managing children at risk of anaphylaxis in order to provide training and support which will create healthy and safe environments for children with allergies, George Raptis, Louise Michaelis, PP15 A new valid and reliable parent and child questionnaire to measure the impact of food protein enterocolitis syndrome on children: the FPIES Quality of Life Questionnaire (FPIESQL), Parent and Child Short Form, Audrey DunnGalvin, Matthew Greenhawt, Carina Venter, Jonathan Hourihane, PP16 An in-depth case study investigation of the experiences of teenagers and young adults in growing up and living with food allergy with emphasis on coping, management and risk, support, and social and self-identity, Evelyn O’Regan, Duncan Cronin, Jonathan Hourihane, Anna O’Reilly, Audrey DunnGalvin, PP17 Cow’s milk protein allergy in Constantine. A retrospective study of 62 cases between 1996 and 2013, Foued Abdelaziz, Dounia Khelifi-Touhami, Nihad Selim, Tahar Khelifi-Touhami, PP18, PP19 Cow’s milk and egg oral immunotherapy in children older than 5 years, Pablo Merida, Ana Mª Plaza, Juan Heber Castellanos, Adrianna Machinena, Montserrat Alvaro Lozano, Jaime Lozano, Olga Dominguez, Monica Piquer, Rosa Jimenez, Mª Teresa Giner, PP20 Professionals’ awareness of management of Cow’s Milk Protein Allergy (CMPA) in North Wales Hospitals, Konstantinos Kakleas, Manohar Joishy, Wendmu Maskele, Huw R. Jenkins, PP21, PP22 Anaphylaxis: the great unknown for teachers. Presentation of a protocol for schools, Mercedes Escarrer, Agustín Madroñero, Maria Teresa Guerra, Juan Carlos Julia, Juan Carlos Cerda, Javier Contreras, Eulalia Tauler, Maria Jesus Vidorreta, Ana Rojo, Silvia Del Valle, PP23 Challenges facing children with food allergies and their parents in out of school activity sectors, Niamh Flynn, PP24 A review of food challenges at a Regional Irish Centre, Gary Foley, Carol Harmon, John Fitzsimons, PP25 The use of epinephrine in infants with anaphylaxis, Krasimira Baynova, Ávila Maria Del Robledo, Labella Marina, PP26, PP27, PP28 Mother’s psychological state predicts the expression of symptoms in food allergic children, Aaron Cortes, Alicia Sciaraffia, Angela Castillo, PP29 The correlation between sIgE towards tree nuts and birch pollen in a Danish Pediatric Allergy Clinic, Nanna Juel-Berg, Kirsten Skamstrup Hansen, Lars Kærgaard Poulsen, PP30 Food allergy in children: evaluation of parents’ use of online social media, Andreia Florina Nita, Ioana Valentina Nenciu, Adina Lazar, Dumitru Oraseanu, PP31 The impact of food allergy on quality of life: FAQLQ questionnaire, Rita Aguiar, Anabela Lopes, Maria J. Paes, Amélia S. Santos, M. A. Pereira-Barbosa, PP32 An unexpected cause of anaphylaxis: potato, Hatice Eke Gungor, Salih Uytun, Umit Murat Sahiner, Yasemin Altuner Torun, PP33 Is it clinical phenotype of allergic diseases determined by sensitisation to food?, Mirjana Zivanovic, Marina Atanasković-Marković, PP34, PP35 Prescribing adrenaline auto-injectors in children in 2014: the data from regional pediatricians, Tina Vesel, Mihaela Nahtigal, Andreja Obermayer-Temlin, Eva Šoster Križnik, Mirjana Maslar, Ruben Bizjak, Marjeta Tomšič-Matic, Sonja Posega-Devetak, Maja Skerbinjek-Kavalar, Mateja Predalič, Tadej Avčin, PP36 Who should have an adrenaline autoinjector? Adherence to the European and French guidelines among 121 allergists from the Allergy Vigilance Network, Guillaume Pouessel, Etienne Beaudouin, Anne M. Moneret-Vautrin, Antoine Deschildre, Allergy Vigilance Network, PP37 Anaphylaxis by Anacardium Occidentale, Marta Viñas, Bartolomé Borja, Nora Hernández, Mª José Castillo, Adriana Izquierdo, Marcel Ibero, PP38 Anaphylaxis with honey in a child, S. Tolga Yavuz, Ali Gungor, Betul Buyuktiryaki, Ozan Koc, Can Naci Kocabas, Faysal Gok, PP39 Evaluation of courses adopted to children on prevention, recognition and management of anaphylaxis, Tina Vesel, Mihaela Nahtigal, PP40 Symptomatic dust mites and shrimp allergy: three pediatric case reports, Filipa Almeida, Susana Lopes, Cristina Madureira, Tânia Lopes, Fernanda Carvalho, PP41 Poor identification rates of nuts by high risk individuals: a call for improved education and support for families, Camille Heming, Emily Garrett, Adam Blackstock, Santanu Maity, Rahul Chodhari, PP42 DAFALL: database of food allergies in the Czech Republic, Simona Belohlavkova, Eliska Kopelentova, Petr Visek, Ivana Setinova, Ivana Svarcova, PP43 Serological cross-reactivity between grass and wheat is not only caused by profilins and CCDs, Sigrid Sjölander, Nora Nilsson, Malin Berthold, Helena Ekoff, Gunilla Hedlin, Magnus Borres, Caroline Nilsson, PP44 Oil body associated proteins in children with nuts allergy. Allergens to consider in IgE-mediated nuts allergy, Loreto González Domínguez, Cristina Muñoz Archidona, Ana Moreira Jorge, Sergio Quevedo Teruel, Teresa Bracamonte Bermejo, Miriam Castillo Fernández, Fernando Pineda de la Losa, Luis Ángel Echeverría Zudaire, PP45, PP46 Protective effect of helicobacter pylori infection against food allergy in children, Olga Vrani, Antigone Mavroudi, Maria Fotoulaki, Maria Emporiadou, Kleomenis Spiroglou, Ioannis Xinias, PP47 Anaphylaxis pathway: A road tryp-tase to success?, Helyeh A. Sadreddini, Mia Warnes, Donna Traves, POSTER VIEWING SESSION 3: Miscell