Mitotic entry in the presence of DNA damage is a widespread property of aneuploidy in yeast

Genetic instability is a hallmark of aneuploidy in budding and fission yeast. All aneuploid yeast strains analyzed to date harbor elevated levels of Rad52-GFP foci, a sign of DNA damage. Here we investigate how continuously elevated levels of DNA damage affect aneuploid cells. We show that Rad52-GFP foci form during S phase, consistent with the observation that DNA replication initiation and elongation are impaired in some aneuploid yeast strains. We furthermore find that although DNA damage is low in aneuploid cells, it nevertheless has dramatic consequences. Many aneuploid yeast strains adapt to DNA damage and undergo mitosis despite the presence of unrepaired DNA leading to cell death. Wild-type cells exposed to low levels of DNA damage exhibit a similar phenotype, indicating that adaptation to low levels of unrepaired DNA is a general property of the cell's response to DNA damage. Our results indicate that by causing low levels of DNA damage, whole-chromosome aneuploidies lead to DNA breaks that persist into mitosis. Such breaks provide the substrate for translocations and deletions that are a hallmark of cancer

Uncertainty in the measurement of indoor temperature and humidity in naturally ventilated dairy buildings as influenced by measurement technique and data variability

[EN] The microclimatic conditions in dairy buildings affect animal welfare and gaseous emissions. Measurements are highly variable due to the inhomogeneous distribution of heat and humidity sources (related to farm management) and the turbulent inflow (associated with meteorologic boundary conditions). The selection of the measurement strategy (number and position of the sensors) and the analysis methodology adds to the uncertainty of the applied measurement technique. To assess the suitability of different sensor positions, in situations where monitoring in the direct vicinity of the animals is not possible, we collected long-term data in two naturally ventilated dairy barns in Germany between March 2015 and April 2016 (horizontal and vertical profiles with 10 to 5 min temporal resolution). Uncertainties related to the measurement setup were assessed by comparing the device outputs under lab conditions after the on-farm experiments. We found out that the uncertainty in measurements of relative humidity is of particular importance when assessing heat stress risk and resulting economic losses in terms of temperature-humidity index. Measurements at a height of approximately 3 m-3.5 m turned out to be a good approximation for the microclimatic conditions in the animal occupied zone (including the air volume close to the emission active zone). However, further investigation along this cross-section is required to reduce uncertainties related to the inhomogeneous distribution of humidity. In addition, a regular sound cleaning (and if possible recalibration after few months) of the measurement devices is crucial to reduce the instrumentation uncertainty in long-term monitoring of relative humidity in dairy barns (C) 2017 The Authors. Published by Elsevier Ltd on behalf of IAgrE.The work was financially supported by the German Federal Ministry of Food and Agriculture (BMEL) through the Federal Office for Agriculture and Food (BLE), grant number 2814ERA02C.Hempel, S.; König, M.; Menz, C.; Janke, D.; Amon, B.; Banhazi, T.; Estellés, F.... (2018). Uncertainty in the measurement of indoor temperature and humidity in naturally ventilated dairy buildings as influenced by measurement technique and data variability. Biosystems Engineering. 166:58-75. https://doi.org/10.1016/j.biosystemseng.2017.11.004S587516

Are transnational tobacco companies' market access strategies linked to economic development models? A case study of South Korea.

Transnational tobacco companies (TTCs) have used varied strategies to access previously closed markets. Using TTCs' efforts to enter the South Korean market from the late 1980s as a case study, this article asks whether there are common patterns in these strategies that relate to the broader economic development models adopted by targeted countries. An analytical review of the existing literature on TTCs' efforts to access emerging markets was conducted to develop hypotheses relating TTCs' strategies to countries' economic development models. A case study of Korea was then undertaken based on analysis of internal tobacco industry documents. Findings were consistent with the hypothesis that TTCs' strategies in Korea were linked to Korea's export-oriented economic development model and its hostile attitude towards foreign investment. A fuller understanding of TTCs' strategies for expansion globally can be derived by locating them within the economic development models of specific countries or regions. Of foremost importance is the need for governments to carefully balance economic and public health policies when considering liberalisation

Anaerobic digestion of whole-crop winter wheat silage for renewable energy production

With biogas production expanding across Europe in response to renewable energy incentives, a wider variety of crops need to be considered as feedstock. Maize, the most commonly used crop at present, is not ideal in cooler, wetter regions, where higher energy yields per hectare might be achieved with other cereals. Winter wheat is a possible candidate because, under these conditions, it has a good biomass yield, can be ensiled, and can be used as a whole crop material. The results showed that, when harvested at the medium milk stage, the specific methane yield was 0.32 m3 CH4 kg–1 volatile solids added, equal to 73% of the measured calorific value. Using crop yield values for the north of England, a net energy yield of 146–155 GJ ha–1 year–1 could be achieved after taking into account both direct and indirect energy consumption in cultivation, processing through anaerobic digestion, and spreading digestate back to the land. The process showed some limitations, however: the relatively low density of the substrate made it difficult to mix the digester, and there was a buildup of soluble chemical oxygen demand, which represented a loss in methane potential and may also have led to biofoaming. The high nitrogen content of the wheat initially caused problems, but these could be overcome by acclimatization. A combination of these factors is likely to limit the loading that can be applied to the digester when using winter wheat as a substrat

Cruise Report: EX-17-11 Gulf of Mexico 2017 (ROV and Mapping)

From November 29, 2017 to December 21, 2017, the NOAA Office of Ocean Exploration and Research (OER) and partners conducted a telepresence-enabled ocean exploration expedition on NOAA Ship Okeanos Explorer to collect critical baseline data and information and to improve knowledge about unexplored and poorly understood deepwater areas of the Gulf of Mexico. The Gulf of Mexico 2017 (EX-17-11) expedition was part of a series of expeditions between 2017 and 2018 that explored deepwater areas in the Gulf of Mexico. During 23 days at sea, 17 remotely operated vehicle (ROV) dives were completed off the Western Florida Escarpment and in the central and western Gulf of Mexico. Over 93 hours of ROV bottom time were logged at depths between 300 and 2,321 meters. Over 20,000 square kilometers of seafloor were mapped. A total of 138 biological and 11 geological samples were collected. The expedition gathered over 280,000 live video views worldwide and the OER website received over 35,600 views. A core onshore science team of over 80 participants from around the world collaborated and supported real-time ocean exploration science. The data associated with this expedition have been archived and are publicly available through the NOAA Archives

Multimedia delivery in the future internet

The term “Networked Media” implies that all kinds of media including text, image, 3D graphics, audio and video are produced, distributed, shared, managed and consumed on-line through various networks, like the Internet, Fiber, WiFi, WiMAX, GPRS, 3G and so on, in a convergent manner [1]. This white paper is the contribution of the Media Delivery Platform (MDP) cluster and aims to cover the Networked challenges of the Networked Media in the transition to the Future of the Internet. Internet has evolved and changed the way we work and live. End users of the Internet have been confronted with a bewildering range of media, services and applications and of technological innovations concerning media formats, wireless networks, terminal types and capabilities. And there is little evidence that the pace of this innovation is slowing. Today, over one billion of users access the Internet on regular basis, more than 100 million users have downloaded at least one (multi)media file and over 47 millions of them do so regularly, searching in more than 160 Exabytes1 of content. In the near future these numbers are expected to exponentially rise. It is expected that the Internet content will be increased by at least a factor of 6, rising to more than 990 Exabytes before 2012, fuelled mainly by the users themselves. Moreover, it is envisaged that in a near- to mid-term future, the Internet will provide the means to share and distribute (new) multimedia content and services with superior quality and striking flexibility, in a trusted and personalized way, improving citizens’ quality of life, working conditions, edutainment and safety. In this evolving environment, new transport protocols, new multimedia encoding schemes, cross-layer inthe network adaptation, machine-to-machine communication (including RFIDs), rich 3D content as well as community networks and the use of peer-to-peer (P2P) overlays are expected to generate new models of interaction and cooperation, and be able to support enhanced perceived quality-of-experience (PQoE) and innovative applications “on the move”, like virtual collaboration environments, personalised services/ media, virtual sport groups, on-line gaming, edutainment. In this context, the interaction with content combined with interactive/multimedia search capabilities across distributed repositories, opportunistic P2P networks and the dynamic adaptation to the characteristics of diverse mobile terminals are expected to contribute towards such a vision. Based on work that has taken place in a number of EC co-funded projects, in Framework Program 6 (FP6) and Framework Program 7 (FP7), a group of experts and technology visionaries have voluntarily contributed in this white paper aiming to describe the status, the state-of-the art, the challenges and the way ahead in the area of Content Aware media delivery platforms

Topological signature of deterministic chaos in short nonstationary signals from an optical parametric oscillator

Although deterministic chaos has been predicted to occur in the triply resonant optical parametric oscillator (TROPO) fifteen years ago, experimental evidence of chaotic behavior in this system has been lacking so far, in marked contrast with most nonlinear systems, where chaos has been actively tracked and found. This situation is probably linked to the high sensitivity of the TROPO to perturbations, which adversely affects stationary operation at high power. We report the experimental observation in this system of a burst of irregular behavior of duration 80 microseconds. Although the system is highly nonstationary over this time interval, a topological analysis allows us to extract a clearcut signature of deterministic chaos from a time series segment of only 9 base cycles (3 microseconds). This result suggests that nonstationarity is not necessarily an obstacle to the characterization of chaos

N 2 O emissions and NO 3 − leaching from two contrasting regions in Austria and influence of soil, crops and climate: a modelling approach

National emission inventories for UN FCCC reporting estimate regional soil nitrous oxide (N 2 O) fluxes by considering the amount of N input as the only influencing factor for N 2 O emissions. Our aim was to deepen the understanding of N 2 O fluxes from agricultural soils, including region specific soil and climate properties into the estimation of emission to find targeted mitigation measures for the reduction of nitrogen losses and GHG emissions. Within this project, N 2 O emissions and nitrate (NO 3 − ) leaching were modelled under spatially distinct environmental conditions in two agricultural regions in Austria taking into account region specific soil and climatic properties, management practices and crop rotations. The LandscapeDNDC ecosystem model was used to calculate N 2 O emissions and NO 3 − leaching reflecting different types of vegetation, management operations and crop rotations. In addition, N input and N fluxes were assessed and N 2 O emissions were calculated. This approach allowed identifying hot spots of N 2 O emissions. Results show that certain combinations of soil type, weather conditions, crop and management can lead to high emissions. Mean values ranged from 0.15 to 1.29 kg N 2 O–N ha −1  year −1 (Marchfeld) and 0.26 to 0.52 kg N 2 O–N ha −1  year −1 (Grieskirchen). Nitrate leaching, which strongly dominated N-losses, often reacted opposite to N 2 O emissions. Larger quantities of NO 3 − were lost during years of higher precipitation, especially if winter barley was cultivated on sandy soils. Taking into account the detected hot spots of N 2 O emissions and NO 3 − leaching most efficient measures can be addressed to mitigate environmental impacts while maximising crop production. © 2018, The Author(s)

KiDS-i-800: Comparing weak gravitational lensing measurements in same-sky surveys

We present a weak gravitational lensing analysis of 815 square degree of $i$-band imaging from the Kilo-Degree Survey (KiDS-$i$-800). In contrast to the deep $r$-band observations, which take priority during excellent seeing conditions and form the primary KiDS dataset (KiDS-$r$-450), the complementary yet shallower KiDS-$i$-800 spans a wide range of observing conditions. The overlapping KiDS-$i$-800 and KiDS-$r$-450 imaging therefore provides a unique opportunity to assess the robustness of weak lensing measurements. In our analysis, we introduce two new `null' tests. The `nulled' two-point shear correlation function uses a matched catalogue to show that the calibrated KiDS-$i$-800 and KiDS-$r$-450 shear measurements agree at the level of $1 \pm 4$\%. We use five galaxy lens samples to determine a `nulled' galaxy-galaxy lensing signal from the full KiDS-$i$-800 and KiDS-$r$-450 surveys and find that the measurements agree to $7 \pm 5$\% when the KiDS-$i$-800 source redshift distribution is calibrated using either spectroscopic redshifts, or the 30-band photometric redshifts from the COSMOS survey.Comment: 24 pages, 20 figures. Submitted to MNRAS. Comments welcom

Photometric redshifts for the Kilo-Degree Survey. Machine-learning analysis with artificial neural networks

We present a machine-learning photometric redshift analysis of the Kilo-Degree Survey Data Release 3, using two neural-network based techniques: ANNz2 and MLPQNA. Despite limited coverage of spectroscopic training sets, these ML codes provide photo-zs of quality comparable to, if not better than, those from the BPZ code, at least up to zphot<0.9 and r<23.5. At the bright end of r<20, where very complete spectroscopic data overlapping with KiDS are available, the performance of the ML photo-zs clearly surpasses that of BPZ, currently the primary photo-z method for KiDS. Using the Galaxy And Mass Assembly (GAMA) spectroscopic survey as calibration, we furthermore study how photo-zs improve for bright sources when photometric parameters additional to magnitudes are included in the photo-z derivation, as well as when VIKING and WISE infrared bands are added. While the fiducial four-band ugri setup gives a photo-z bias $\delta z=-2e-4$ and scatter $\sigma_z<0.022$ at mean z = 0.23, combining magnitudes, colours, and galaxy sizes reduces the scatter by ~7% and the bias by an order of magnitude. Once the ugri and IR magnitudes are joined into 12-band photometry spanning up to 12 $\mu$, the scatter decreases by more than 10% over the fiducial case. Finally, using the 12 bands together with optical colours and linear sizes gives $\delta z<4e-5$ and $\sigma_z<0.019$. This paper also serves as a reference for two public photo-z catalogues accompanying KiDS DR3, both obtained using the ANNz2 code. The first one, of general purpose, includes all the 39 million KiDS sources with four-band ugri measurements in DR3. The second dataset, optimized for low-redshift studies such as galaxy-galaxy lensing, is limited to r<20, and provides photo-zs of much better quality than in the full-depth case thanks to incorporating optical magnitudes, colours, and sizes in the GAMA-calibrated photo-z derivation.Comment: A&A, in press. Data available from the KiDS website http://kids.strw.leidenuniv.nl/DR3/ml-photoz.php#annz