Pengembangan Buku Ajar Tabm Berbasis Penelitian untuk Mahasiswa S1 Jurusan Biologi Universitas Negeri Gorontalo

Analysis and Techniques of Molecular Biology (TABM) Subject need of competence for using a set of tools and materials in molecular biology laboratory. Department of Biology, State University of Gorontalo is planned to apply TABM courses for undegraduate students but no available a complete molecular biology laboratory. The development of research-based of TABM textbook is one solution to the student attends TABM lectures. The development of a research-based of TABM textbook was based on Borg and Gall development model. The product is validated by instructional media expert, subject matter expert and lecturer. The readability test conducted to ten students. Results of validation by media experts, subject matter experts and lecturers practitioners respectively are 93%, 92% and 89.2% with a valid category. While the test results readability of students is 87% with valid category.Perkuliahan Teknik Analisis Biologi Molekuler (TABM) sarat dengan penggunaan set alat dan bahan laboratorium Biologi molekuler yang lengkap. Jurusan Biologi Universitas Negeri Gorontalo diketahui akan menerapkan matakuliah TABM untuk mahasiswa S1 namun belum memiliki set laboratorium Biologi molekuler yang lengkap. Pengembangan buku ajar TABM berbasis penelitian merupakan salah satu solusi untuk mahasiswa mengikuti perkuliahan TABM. Pengembangan buku ajar TABM berbasis penelitian menggunakan model pengembangan Borg and Gall. Buku ajar yang dikembangkan divalidasi oleh ahli media pembelajaran, ahli materi dan dosen praktisi. Buku ajar di uji keterbacaan oleh 10 mahasiswa. Hasil validasi ahli media pembelajaran, ahli materi dan dosen praktisi secara berurutan adalah 93%, 92% dan 89,2% dengan kategori valid. Sedangkan hasil uji keterbacaan mahasiswa adalah 87% dengan kategori valid

Study Pencahayaan Alami pada Rumah Limas Panggung Palembang (Simulasi dengan Program Ecotect 5.0)

Sunlight as the largest source of natural light is widely used by humans to carry out their daily activities. Natural light is efficient and saves energy because it does not use electricity. Sometimes humans as light users do not realize how huge the importance of natural light in performing everyday activities as visual comfort. In this study, simulations are conducted on  Limas Stilt Houses Palembang. This is due to Limas Stilt Houses has existed since Palembang Sultanate era. This study focuses on research related to the opening on the wall of the elements of these houses through the simulation of natural light. The method used in this research is descriptive analysis method based on engineering and architecture discipline of fields. From the simulation results obtained that the \u27gegajah\u27 space produced very little natural light ranges from 15-30 lux. This is due to gegajah space is located in the middle of the house and just rely on natural light from the \u27bengkilas\u27  and \u27pawon\u27 rom. While \u27bengkilas\u27 and \u27pawon\u27 sufficient range of 300-325 lux natural light

Outbreak of Peritonitis in a Continuous Ambulatory Peritoneal Dialysis Population Following the Use of Contaminated Peritoneal Dialysis Fluids

Introduction: Most cases of peritonitis during continuous ambulatory peritoneal dialysis (CAPD) are attributed to breaches of the aseptic technique. In this report, we describe an outbreak of CAPD related peritonitis that followed the use of potentially contaminated PD fluid. Outbreak report: CAPD was introduced in the adult nephrology unit at Soba University Hospital in December 2008 with fairly satisfactory functioning and results. In June 2009, we obtained a new supply of PD fluids and started using it. Soon afterwards, a mould was found inside a new unused dialysate bag. During the following days, six patients were diagnosed to have PD related peritonitis. All patients in the unit were immediately shifted to another brand of PD fluids. Enquiry revealed that this supply of PD fluid was stored in a warm and humid environment. We surveyed the 1469 bags in the hospital stock and found another three bags (0.2%) that contained visible mould by the naked eye. The four contaminated bags contained 2.5% dextrose and were from different batches. All of them had lost the negative vacuum between the inner bag and the outer plastic envelope, but had no obvious tears in the envelope and no obvious fluid leakage. Our peritonitis rate before the outbreak was one episode in 21.7 patient-months. This sharply rose to one episode in 2.5 patient-months during the month of the outbreak, and dropped down to one episode in 17.8 patient-months in the 6-months following the outbreak. Conclusion: Contamination of PD fluids can occur during handling and storage. Patients should meticulously examine each bag before usage. Any bag that has lost the vacuum between the inner bag and its outer envelope is potentially breached and should not be used. Keywords: Contamination; Fungal peritonitis; Outbreak; Penicillium spp.; Peritoneal dialysi

Calibrated radar-derived rainfall data for rainfall-runoff modeling.

This study focuses on a technique to improve runoff modeling based on radar-derived rainfall and hydrological model for the whole watershed. GIS tools were used to provide the model parameters for the Upper Bernam River Basin (1090 km2), Malaysia. Virtual rainfall stations are created throughout the UBRB watershed. The rainfall data for these stations are estimated from raw weather radar data using newly developed program called RaDeR ver1.0. For this study, estimated radar rainfall data from Subang weather radar stations were compared and calibrated with actual rain gauge data. Radar-derived rainfall calibration model developed for Subang radar station was y=0.8772x. According to the model developed, the radar rainfall calibration factor (RCf) can be identified as 0.8772. The original estimated radar derived rainfall data should be adjusted before using the calibration factor (RCf). The model gives better correlation when adjusted radar values were used instead of the original radar rainfall values. The model calibration factor increased from 0.464 with R2 of 0.2759** to 0.8772 with R2 of 0.3655***. Finally, the virtual rainfall stations created throughout the river basin produced a more representative rainfall distribution. It is believed that watershed river flow can be better estimated by using radar-derived rainfall data

Synthesis and Characterization of Silver-Coated Polymeric Scaffolds for Bone Tissue Engineering: Antibacterial and In Vitro Evaluation of Cytotoxicity and Biocompatibility

In bone tissue engineering, multifunctional composite materials are very challenging. Bone tissue engineering is an innovative technique to develop biocompatible scaffolds with suitable orthopedic applications with enhanced antibacterial and mechanical properties. This research introduces a polymeric nanocomposite scaffold based on arabinoxylan-co-acrylic acid, nano-hydroxyapatite (nHAp), nano-aluminum oxide (nAl₂O₃), and graphene oxide (GO) by free-radical polymerization for the development of porous scaffolds using the freeze-drying technique. These polymeric nanocomposite scaffolds were coated with silver (Ag) nanoparticles to improve antibacterial activities. Together, nHAp, nAl₂O₃, and GO enhance the multifunctional properties of materials, which regulate their physicochemical and biomechanical properties. Results revealed that the Ag-coated polymeric nanocomposite scaffolds had excellent antibacterial properties and better microstructural properties. Regulated morphological properties and maximal antibacterial inhibition zones were found in the porous scaffolds with the increasing amount of GO. Moreover, the nanosystem and the polymeric matrix have improved the compressive strength (18.89 MPa) and Young’s modulus (198.61 MPa) of scaffolds upon increasing the amount of GO. The biological activities of the scaffolds were investigated against the mouse preosteoblast cell lines (MC3T3-E1) and increasing the quantities of GO helps cell adherence and proliferation. Therefore, our findings showed that these silver-coated polymeric nanocomposite scaffolds have the potential for engineering bone tissue

Buletin Pa'biritta LPMP Sulawesi Selatan nomor 19 tahun X 2017

Buletin Pa'birita nomor 19 ini terbit setelah beberapa lama berhenti selama beberapa waktu. Penerbitanya merupakan permintaan dari widyaiswara, guru dan tenaga kependidikan yang ingin karyanya di publikasikan. Buletin ini menyajikan tulisan yang membahasa penjaminan mutu pendidikan, program induksi, efek samping haemodialisis, teknologi pembelajaran, Praktikum pembelajaran IPA, Fungsi dan ragam bahasa, Penyelesaian perkalian, dan pappaseng toriolota

A whole genome analyses of genetic variants in two Kelantan Malay individuals

The sequencing of two members of the Royal Kelantan Malay family genomes will provide insights on the Kelantan Malay whole genome sequences. The two Kelantan Malay genomes were analyzed for the SNP markers associated with thalassemia and Helicobacter pylori infection. Helicobacter pylori infection was reported to be low prevalence in the north-east as compared to the west coast of the Peninsular Malaysia and beta-thalassemia was known to be one of the most common inherited and genetic disorder in Malaysia.By combining SNP information from literatures, GWAS study and NCBI ClinVar, 18 unique SNPs were selected for further analysis. From these 18 SNPs, 10 SNPs came from previous study of Helicobacter pylori infection among Malay patients, 6 SNPs were from NCBI ClinVar and 2 SNPs from GWAS studies. The analysis reveals that both Royal Kelantan Malay genomes shared all the 10 SNPs identified by Maran (Single Nucleotide Polymorphims (SNPs) genotypic profiling of Malay patients with and without Helicobacter pylori infection in Kelantan, 2011) and one SNP from GWAS study. In addition, the analysis also reveals that both Royal Kelantan Malay genomes shared 3 SNP markers; HBG1 (rs1061234), HBB (rs1609812) and BCL11A (rs766432) where all three markers were associated with beta-thalassemia.Our findings suggest that the Royal Kelantan Malays carry the SNPs which are associated with protection to Helicobacter pylori infection. In addition they also carry SNPs which are associated with beta-thalassemia. These findings are in line with the findings by other researchers who conducted studies on thalassemia and Helicobacter pylori infection in the non-royal Malay population.Wan Khairunnisa Wan Juhari, Nur Aida Md Tamrin, Mohd Hanif Ridzuan Mat Daud, Hatin Wan Isa, Nurfazreen Mohd Nasir, Sathiya Maran, Nur Shafawati Abdul Rajab, Khairul Bariah Ahmad Amin Noordin, Nik Norliza Nik Hassan, Rick Tearle, Rozaimi Razali, Amir Feisal Merican and Bin Alwi Zilfali

Prevalence and risk factors for diabetic neuropathy and painful diabetic neuropathy in primary and secondary health care in Qatar.

AIMS/INTRODUCTION:This study determined the prevalence and risk factors for DPN and pDPN in patients with type 2 diabetes (T2D) in primary health care (PHC) and secondary health care (SHC) in Qatar. MATERIALS AND METHODS:This is a cross-sectional multi-center study. Adults with T2D were randomly enrolled from four PHC centres and two Diabetes Centres in SHC in Qatar. Subjects underwent assessment of clinical and metabolic parameters, DPN and pDPN. RESULTS:1,386 subjects with T2D (297 from PHC and 1,089 from SHC) were recruited. The prevalence of DPN (14.8% vs 23.9%, P=0.001) and pDPN (18.1% vs 37.5%, P<0.0001) was significantly lower in PHC compared to SHC, whilst those with DPN at high risk for DFU (31.8% vs 40.0%, P=0.3) was comparable. The prevalence of undiagnosed DPN (79.5% vs 82.3%, P=0.66) was comparably high but undiagnosed pDPN (24.1% vs 71.5%, P<0.0001) was lower in PHC compared to SHC. The odds of DPN and pDPN increased with age and diabetes duration and DPN increased with poor glycemic control, hyperlipidemia and hypertension, whilst pDPN increased with obesity and reduced physical activity. CONCLUSIONS:The prevalence of DPN and pDPN in T2D is lower in PHC compared to SHC and is attributed to overall better control of risk factors and referral bias due to patients with poorly managed complications being referred to SHC. However, ~80% of patients had not been previously diagnosed with DPN in PHC and SHC. Further, we identify a number of modifiable risk factors for PDN and pDPN

The Scales Project, a cross-national dataset on the interpretation of thermal perception scales

Thermal discomfort is one of the main triggers for occupants’ interactions with components of the built environment such as adjustments of thermostats and/or opening windows and strongly related to the energy use in buildings. Understanding causes for thermal (dis-)comfort is crucial for design and operation of any type of building. The assessment of human thermal perception through rating scales, for example in post-occupancy studies, has been applied for several decades; however, long-existing assumptions related to these rating scales had been questioned by several researchers. The aim of this study was to gain deeper knowledge on contextual influences on the interpretation of thermal perception scales and their verbal anchors by survey participants. A questionnaire was designed and consequently applied in 21 language versions. These surveys were conducted in 57 cities in 30 countries resulting in a dataset containing responses from 8225 participants. The database offers potential for further analysis in the areas of building design and operation, psycho-physical relationships between human perception and the built environment, and linguistic analyses

Regional and experiential differences in surgeon preference for the treatment of cervical facet injuries: a case study survey with the AO Spine Cervical Classification Validation Group

Purpose: The management of cervical facet dislocation injuries remains controversial. The main purpose of this investigation was to identify whether a surgeon’s geographic location or years in practice influences their preferred management of traumatic cervical facet dislocation injuries. Methods: A survey was sent to 272 AO Spine members across all geographic regions and with a variety of practice experience. The survey included clinical case scenarios of cervical facet dislocation injuries and asked responders to select preferences among various diagnostic and management options. Results: A total of 189 complete responses were received. Over 50% of responding surgeons in each region elected to initiate management of cervical facet dislocation injuries with an MRI, with 6 case exceptions. Overall, there was considerable agreement between American and European responders regarding management of these injuries, with only 3 cases exhibiting a significant difference. Additionally, results also exhibited considerable management agreement between those with ≤ 10 and &gt; 10&nbsp;years of practice experience, with only 2 case exceptions noted. Conclusion: More than half of responders, regardless of geographical location or practice experience, identified MRI as a screening imaging modality when managing cervical facet dislocation injuries, regardless of the status of the spinal cord and prior to any additional intervention. Additionally, a majority of surgeons would elect an anterior approach for the surgical management of these injuries. The study found overall agreement in management preferences of cervical facet dislocation injuries around the globe