Sterilization methods for humam teeth

ABSTRACT Appropriate sterilization methods for dental substrate are necessary so that extracted teeth can be used in laboratory activities, since potentially pathogenic microorganisms may be present even if teeth have been cleaned. Objective Evaluating the sterilization process of human teeth performed by microwave irradiation and autoclave sterilization. Methods Fifty human premolar teeth were sectioned in two portions and distributed in two test groups: G1 - autoclave sterilization for 20 minutes, 1Kgf/cm2, at 127ºC; G2 - Microwave irradiation in a recipient containing 100ml of distilled water, 900W for 5 minutes. Before and after the decontamination process, all teeth were immersed in Brain Heart Infusion (BHI) broth to verify their contamination (positive control) and decontamination potential respectively. Results The statistical analysis of the Friedman test (p <0.05) showed that all the tested sterilization methods were statistically effective. Conclusion These results show that both autoclaving and microwave irradiation are effective alternatives for the sterilization of human teeth

Microwave and autoclave usage in human teeth sterilization

Objective: evaluate the sterilization of human teeth irradiated by microwaves. Methods: Sixty human third molars are divided into three groups (n = 20): G1 without sterilization (negative control); G2 - autoclaving for 20 minutes 1Kgf/cm2 at 120 ° C (positive control); G3 - sterilization in a microwave vessel containing 200ml of distilled water in a microwave irradiated at 650W for 3 minutes. Results: No culture media of G2 and G3 presented contamination after autoclaving and microwave sterilization. Conclusion: Autoclave sterilization and microwave sterilization were effective decontamination methods under the experimental conditions tested

Eficácia da barreira intracanal contra ainfiltração microbiana em dentes preparados para pino intrarradicular [Efficacy of intracanal barrier against microbial infiltration in teeth prepared for intraradicular post]

Introduction: Intraradicular posts are recommended to improve retention of artificial crowns and distribute intraoral forces along the root. If the space created by partial removal of the filling is not filled properly, massive infiltration of microorganisms from the oral cavity can occur. Objective: The objective of the present study was to evaluate the effectiveness against bacterial infiltration of an intracanal barrier placed directly over the remainder of the root canal filling, after space preparation for the post. Methods: Seventy-two single-rooted human teeth were instrumented, filled and randomly divided into three experimental groups and two control groups. Group 1 received no additional treatment after filling and space preparation, while Groups 2 and 3 received a barrier composed of temporary sealing material 1.0 and 2.0 mm thick, respectively. A culture of Enterococcus faecalis was inoculated in the spaces prepared to receive the intraradicular post, every three days, for a period of 60 days. Infiltration was evaluated daily by the turbidity of the culture medium. Results and Conclusion: There was bacterial infiltration in the three experimental groups, being significantly higher and occurring more quickly in G1 (p0.05) was found between G2 and G3, in relation to the rate and period of infiltration