Stipa glareosa (Poaceae) in the Republic of Buryatia (Russia)

Stipa glareosa P. A. Smirn. (sect. Smirnovia Tzvel.) is reported for the first time from the Republic of Buryatia (Russia). Its stations are located at the northern limit of its general distribution range. The taxonomy, distribution and habitat preferences of the species are given, along with its population size at each new locality

Трансформация культуры чтения в цифровую эпоху как показатель цивилизационных сдвигов

Материалы X Международной научной конференции 25–26 мая 2017 г

Findings to the flora of Russia and adjacent countries: New national and regional vascular plant records, 4

With this paper we continue a new annual series, the main purpose of which is to make significant floristic findings from Russia and neighboring countries more visible in Russia and abroad. In total, this paper presents new records for 48 vascular plant species from 6 Eurasian countries, obtained during field explorations, as well as during taxonomic revisions of herbarium materials. For the first time, a new locality of Leontopodium leiolepis is recorded for Russia, Rheum uzengukuushi for China, Rorippa prolifera for Lithuania, Lappula marginata for Kyrgyzstan and Tajikistan, Anthriscus caucalis, Chenopodium ficifolium, Euphorbia prostrata for Uzbekistan, Adonis × hybrida, Potamogeton × franconicus, Solidago × niederederi for the Asian part of Russia, Echinochloa esculenta, Poa jamalinensis, Puccinellia poecilantha for Siberia, Potentilla intermedia for the Caucasus, Rhynchospora alba for the Russian part of Altai, Poa sphondylodes, Veronica beccabunga for Eastern Siberia, Asclepias syriaca for the Republic of Altai, Chimaphila umbellata, Orobanche korshinskyi, Veronica scutellata for the Republic of Buryatia, Cirsium alatum, Thalictrum simplex for the Republic of Crimea, Thymus rariflorus, Th. terekensis for the Republic of Ingushetia, Berberis thunbergii, Crataegus maximowiczii, Prunus serotina for the Republic of Mordovia, Oenothera villosa for the Republic of Tatarstan, Astragalus sulcatus, Galium mollugo for the Republic of Tyva, Phragmites altissimus for the Chelyabinsk Region, Senecio dubitabilis for the Magadan Region, Asclepias syriaca, Galatella villosa, Potentilla recta for the Novosibirsk Region, Dodartia orientalis for the Omsk Region, Viola hultenii for the Sakhalin Region, Phragmites tzvelevii for the Samara Region and the Middle Volga, Jacobaea ferganensis for the Samara Region, Carex media, Impatiens parviflora for the Tyumen Region. There are some more findings which are not new for the region but they contribute significantly to the understanding of species distribution

IAPT/IOPB chromosome data 16

[EN] Inula helenioides DC., I. langeana Beck, I. maletii Maire, I. montana L., I. oculus-christi L., I. salicina L. (Asteraceae)

Stipa glareosa (Poaceae) in the Republic of Buryatia (Russia)

Stipa glareosa P. A. Smirn. (sect. Smirnovia Tzvel.) is reported for the first time from the Republic of Buryatia (Russia). Its stations are located at the northern limit of its general distribution range. The taxonomy, distribution and habitat preferences of the species are given, along with its population size at each new locality

Genetic and morphological evidence for introgression between three species of willows

Background: Hybridization and introgression are said to occur relatively frequently in plants, and in particular among different species of willows. However, data on the actual frequency of natural hybridization and introgression is rare. Here, we report the first fine-scale genetic analysis of a contact zone shared between the three basket willow species, Salix dasyclados, S. schwerinii and S. viminalis in the vicinity of the Lake Baikal in Southern Siberia. Individuals were sampled in fourteen populations and classified as pure species or hybrids based on a set of morphological characters. They were then genotyped at 384 nuclear SNP and four chloroplast SSR loci. The STRUCTURE and NewHybrids softwares were used to estimate the frequency and direction of hybridization using genotypic data at the nuclear SNP loci. Results: As many as 19 % of the genotyped individuals were classified as introgressed individuals and these were mainly encountered in the centre of the contact zone. All introgressed individuals were backcrosses to S. viminalis or S. schwerinii and no F1 or F2 hybrids were found. The rest of the genotyped individuals were classified as pure species and formed two clusters, one with S. schwerinii individuals and the other with S. viminalis and S. dasyclados individuals. The two clusters were significantly genetically differentiated, with F-ST = 0.333 (0.282-0.382, p < 0.001). In contrast, for the chloroplast haplotypes, no genetic differentiation was observed as they were completely shared between the species. Based on morphological classification only 5 % of the individuals were classified as introgressed individuals, which was much less than what was detected using genotypic data. Conclusions: We have discovered a new willow hybrid zone with relatively high frequency of introgressed individuals. The low frequency of F1 hybrids indicates that ongoing hybridization is limited, which could be because of the presence of reproductive barriers or simply because the conditions are not favorable for hybridization. We further conclude that in order to get a complete picture of the species composition of a hybrid zone it is necessary to use a combination of morphological characters and genetic data from both nuclear and chloroplast markers

Phyllobacterium zundukense sp. nov., a novel species of rhizobia isolated from root nodules of the legume species Oxytropis triphylla (Pall.) Pers.

Gram-negative strains Tri-36, Tri-38, Tri-48T and Tri-53 were isolated from root nodules of the relict legume Oxytropis triphylla (Pall.) Pers. originating from Zunduk Cape (Baikal Lake region, Russia). 16S rRNA gene sequencing showed that the novel isolates were phylogenetically closest to the type strains Phyllobacterium sophorae LMG 27899(T), Phyllobacterium brassicacearum LMG 22836(T), Phyllobacterium endophyticum LMG 26470(T) and Phyllobacterium bourgognense LMG 22837(T) while similarity levels between the isolates and the most closely related strain P. endophyticum LMG 26470(T) were 98.899.5 %. The recA and glnII genes of the isolates showed highest sequence similarities with P. sophorae LMG 27899(T) (95.4 and 89.5 %, respectively) and P. brassicacearum LMG 22836(T) (91.4 and 85.1 %, respectively). Comparative analysis of phenotypic properties between the novel isolates and the closest reference strains P. sophorae LMG 27899(T), P. brassicacearum LMG 22836(T) and P. endophyticum LMG 26470(T) was performed using a microassay system. Average nucleotide identities between the whole genome sequences of the isolates Tri-38 and Tri-48(T) and P. sophorae LMG 27899(T), P. brassicacearum LMG 22836(T) and P. endophyticum LMG 26470(T) ranged from 79.23% for P. endophyticum LMG 26470(T) to 85.74% for P. sophorae LMG 27899(T). The common nodABC genes required for legume nodulation were absent from strains Tri-38 and Tri-48(T), although some other symbiotic nod and fix genes were detected. On the basis of genotypic and phenotypic analysis, a novel species, Phyllobacterium zundukense sp. nov. (type strain Tri-48(T) = LMG 30371(T) = RCAM 03910(T)), is proposed

Bosea caraganae sp. nov. a new species of slow-growing bacteria isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia

Two Gram-stain-negative strains, RCAM04680(T) and RCAM04685, were isolated from root nodules of the relict legume Caragana jubata (Pall.) Poir. originating from the south-western shore of Lake Khuvsgul (Mongolia). The 16S rRNA gene (rrs) sequencing data showed that these novel isolates belong to the genus Bosea and are phylogenetically closest to the type strains Bosea lathyri LMG 26379(T), Bosea vaviloviae LMG 28367(T), Bosea massiliensis LMG 26221(T) and Bosea lupini LMG 26383(T) (the rrs-similarity levels were 98.7-98.8 %). The recA gene of strain RCAM04680(T) showed the highest sequence similarity to the type strain B. lupini LMG 26383(T) (95.4 %), while its atpD gene was closest to that of B. lathyri LMG 26379(T) (94.4 %). The ITS, dnaK and gyrB sequences of this isolate were most similar to the B. vaviloviae LMG 28367(T) (86.8% for ITS, 90.4% for the other genes). The most abundant fatty acid was C-18:1 omega 7c (40.8 %). The whole genomes of strains RCAM04680(T) and RCAM04685 were identical (100% average nucleotide identity). The highest average nucleotide identity value (82.8 %) was found between the genome of strain RCAM04680(T) and B. vaviloviae LMG 28367(T). The common nodABC genes required for legume nodulation were absent in both strains; however, some other symbiotic nol, nod, nif and fix genes were detected. Based on the genetic study, as well as analyses of the whole-cell fatty acid compositions and phenotypic properties, a new species, Bosea caraganae sp. nov. (type strain RCAM04680(T) (=LMG 31125(T)), is proposed

Contribution to the flora of Asian and European countries: new national and regional vascular plant records, 3

The paper presents new records for 18 vascular plant species from six Eurasian countries. Three taxa (Lepidium densiflorum, Stipa czerepanovii, Xanthium albinum) are reported from Kazakhstan, one (Ranunculus schmakovii) from Mongolia, three (Dianthus campestris, Ranunculus kauffmannii, Viola suavis) from Poland, five (Eragrostis amurensis, Linum catharticum, Ludwigia peploides subsp. stipulacea, Pyrethrum mikeschinii, Solidago canadensis) from Tajikistan, five (Clinopodium menthifolium, Juncus effusus, Mollugo cerviana, Poa sphondylodes, Vulpia myuros) from Russia, and one (Orobanche alba subsp. xanthostigma) from Georgia. Ten of the taxa presented (Clinopodium menthifolium, Dianthus campestris, Eragrostis amurensis, Juncus effusus, Lepidium densiflorum, Mollugo cerviana, Solidago canadensis, Viola suavis, Vulpia myuros and Xanthium albinum) are regarded as alien to the studied areas, whereas the remaining eight are native elements to the flora of the countries. For each species, synonyms, general distribution, habitat preferences, taxonomy with remarks on recognition and differentiation of the species from the most similar occurring in a given country, as well as a list of localities recorded (often far from the previously known areas), are presented

Contribution to the flora of Asian and European countries: new national and regional vascular plant records, 3

The paper presents new records for 18 vascular plant species from six Eurasian countries. Three taxa (Lepidium densiflorum, Stipa czerepanovii, Xanthium albinum) are reported from Kazakhstan, one (Ranunculus schmakovii) from Mongolia, three (Dianthus campestris, Ranunculus kauffmannii, Viola suavis) from Poland, five (Eragrostis amurensis, Linum catharticum, Ludwigia peploides subsp. stipulacea, Pyrethrum mikeschinii, Solidago canadensis) from Tajikistan, five (Clinopodium menthifolium, Juncus effusus, Mollugo cerviana, Poa sphondylodes, Vulpia myuros) from Russia, and one (Orobanche alba subsp. xanthostigma) from Georgia. Ten of the taxa presented (Clinopodium menthifolium, Dianthus campestris, Eragrostis amurensis, Juncus effusus, Lepidium densiflorum, Mollugo cerviana, Solidago canadensis, Viola suavis, Vulpia myuros and Xanthium albinum) are regarded as alien to the studied areas, whereas the remaining eight are native elements to the flora of the countries. For each species, synonyms, general distribution, habitat preferences, taxonomy with remarks on recognition and differentiation of the species from the most similar occurring in a given country, as well as a list of localities recorded (often far from the previously known areas), are presented