Why bad ideas are a good idea

What would happen if we wrote an Abstract that was the exact opposite of what the paper described? This is a bad idea, but it makes us think more carefully than usual about properties of Abstracts. This paper describes BadIdeas, a collection of techniques that uses ???bad??? or ???silly??? ideas to inspire creativity, explore design domains and teach critical thinking in interaction design. We describe the approach, some evidence, how it is performed in practice and experience in its use.published or submitted for publicationis peer reviewe

Using mixed methods for analysing culture : The Cultural Capital and Social Exclusion project

This paper discusses the use of material generated in a mixed method investigation into cultural tastes and practices, conducted in Britain from 2003 to 2006, which employed a survey, focus groups and household interviews. The study analysed the patterning of cultural life across a number of fields, enhancing the empirical and methodological template provided by Bourdieu’s Distinction. Here we discuss criticisms of Bourdieu emerging from subsequent studies of class, culture and taste, outline the arguments related to the use of mixed methods and present illustrative results from the analysis of these different types of data. We discuss how the combination of quantitative and qualitative methods informed our analysis of cultural life in contemporary Britain. No single method was able to shed light on all aspects of our inquiry, lending support to the view that mixing methods is the most productive strategy for the investigation of complex social phenomena

Feasible low bone density condition for assessing bioactivity in ex-in vivo and in vivo studies

Objective: To choose a critical animal model for assessments of bone repair with implant installation by comparing senile rats (SENIL) to young ovariectomized rats (OXV). Methodology: For the ex-in vivo study, the femurs were precursors for bone marrow mesenchymal stem cells. Cellular responses were performed, including cell viability, gene expression of osteoblastic markers, bone sialoprotein immunolocalization, alkaline phosphatase activity, and mineralized matrix formation. For the in vivo study, the animals received implants in the region of the bilateral tibial metaphysis for histometric, microtomography, reverse torque, and confocal microscopy. Results: Cell viability showed that the SENIL group had lower growth than OVX. Gene expression showed more critical responses for the SENIL group (p<0.05). The alkaline phosphatase activity obtained a lower expression in the SENIL group, as for the mineralization nodules (p<0.05). The in vivo histological parameters and biomechanical analysis showed lower data for the SENIL group. The confocal microscopy indicated the presence of a fragile bone in the SENIL group. The microtomography was similar between the groups. The histometry of the SENIL group showed the lowest values (p<0.05). Conclusion: In experimental studies with assessments of bone repair using implant installation, the senile model promotes the most critical bone condition, allowing a better investigation of the properties of biomaterials and topographic changes

Dissecting EPPIN protease inhibitor domains in sperm motility and fertilizing ability: Repercussions for male contraceptive development

EPPIN (epididymal protease inhibitor) is a mammalian conserved sperm-binding protein displaying an N-terminal WFDC (whey-acidic protein four-disulfide core) and a C-terminal Kunitz protease inhibitor domains. EPPIN plays a key role in regulating sperm motility after ejaculation via interaction with the seminal plasma protein SEMG1 (semenogelin-1). EPPIN ligands targeting the SEMG1 binding site in the Kunitz domain are under development as male contraceptive drugs. Nevertheless, the relative contributions of EPPIN WFDC and Kunitz domains to sperm function remain obscure. Here, we evaluated the effects of antibodies targeting specific epitopes in EPPIN's WFDC (Q20E antibody, Gln20-Glu39 epitope) and Kunitz (S21C and F21C antibodies, Ser103-Cys123 and Phe90-C110 epitopes, respectively) domains on mouse sperm motility and fertilizing ability. Computer-assisted sperm analysis showed that sperm co-incubation with S21C antibody (but not F21C antibody) lowered progressive and hyperactivated motilities and impaired kinematic parameters describing progressive (straight-line velocity; VSL, average path velocity; VAP and straightness; STR) and vigorous sperm movements (curvilinear velocity; VCL, amplitude of lateral head movement; ALH, and linearity; LIN) compared with control. Conversely, Q20E antibody-induced milder inhibition of progressive motility and kinematic parameters (VAP, VCL and ALH). Sperm co-incubation with S21C or Q20E antibodies affected in vitro fertilization as revealed by reduced cleavage rates, albeit without changes in capacitation-induced tyrosine phosphorylation. In conclusion, we show that targeting specific epitopes in EPPIN Kunitz and WFDC domains inhibits sperm motility and capacitation-associated events, which decrease their fertilizing ability; nevertheless, similar observations in vivo remain to be demonstrated. Simultaneously targeting residues in S21C and Q20E epitopes is a promising approach for the rational design of EPPIN-based ligands with spermostatic activity.Fil: Silva, Alan A. S.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Raimundo, Tamiris R. F.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Mariani, Noemia A. P.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Kushima, Hélio. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Avellar, Maria Christina W.. Universidade Federal de Sao Paulo; BrasilFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Paula Lopes, Fabíola F.. Universidade Federal de Sao Paulo; BrasilFil: Moura, Marcelo T.. Universidade Federal de Sao Paulo; BrasilFil: Silva, Erick J. R.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasi

Correlação entre comportamentos de risco à saúde, ansiedade e depressão em adolescentes

The objectives of the study were to describe the proportions of health risk behaviors, anxiety and depression in adolescents of a private school in the municipality of Marechal Cândido Rondon - PR. The study is descriptive and correlational in nature. The sample was composed of 112 students (secondary and middle school) with a mean age of 14.1 years (±2.0). They answered questions about sociodemographic information, level of physical activity (IPAQ-short version), tobacco and alcohol consumption (YRBSS), anxiety (SCARED) and depression (CDI). The final sample consisted of students from upper socioeconomic classes (95.5%), of which 66 were from primary school and 46 from high school, 53.6% female. There were high levels of insufficiency of physical activity (53.5%) and risk of anxiety (16.9%). Even higher proportions were observed in females for insufficiency of Physical Activity (65%) and risk of anxiety (26.7%). There were significant negative association between participation in moderate and vigorous physical activity and risk of anxiety for the total sample (r=-0.215, p=0.02), and for the secondary school students (r =-0.245, p=0.047). Adolescents with higher alcohol consumption portrayed significant positive association with risk of depression (r=0.245, p=0.006). Overall, adolescents with frequent alcohol consumption behavior have low levels of physical activity and high levels of anxiety. Interventions for the reduction of mental disorders should stimulate the increase in habitual physical activity levels, especially in females and reduction in the consumption of alcoholic beverages in males

Alice em Busca da sua Caixa de Brinquedos: um jogo para apoio a tomada de decisões

This article presents the game  Alice in Search of its Toy Box, which  is being developed through the Alice development environment, and aims to monitor the development of cognitive attributes on the player, such as:  memorization,  planning, decision making (reasoning)

Transcriptional activity, chromosomal distribution and expression effects of transposable elements in Coffea genomes

Plant genomes are massively invaded by transposable elements (TEs), many of which are located near host genes and can thus impact gene expression. In flowering plants, TE expression can be activated (de-repressed) under certain stressful conditions, both biotic and abiotic, as well as by genome stress caused by hybridization. In this study, we examined the effects of these stress agents on TE expression in two diploid species of coffee, Coffea canephora and C. eugenioides, and their allotetraploid hybrid C. arabica. We also explored the relationship of TE repression mechanisms to host gene regulation via the effects of exonized TE sequences. Similar to what has been seen for other plants, overall TE expression levels are low in Coffea plant cultivars, consistent with the existence of effective TE repression mechanisms. TE expression patterns are highly dynamic across the species and conditions assayed here are unrelated to their classification at the level of TE class or family. In contrast to previous results, cell culture conditions per se do not lead to the de-repression of TE expression in C. arabica. Results obtained here indicate that differing plant drought stress levels relate strongly to TE repression mechanisms. TEs tend to be expressed at significantly higher levels in non-irrigated samples for the drought tolerant cultivars but in drought sensitive cultivars the opposite pattern was shown with irrigated samples showing significantly higher TE expression. Thus, TE genome repression mechanisms may be finely tuned to the ideal growth and/or regulatory conditions of the specific plant cultivars in which they are active. Analysis of TE expression levels in cell culture conditions underscored the importance of nonsense-mediated mRNA decay (NMD) pathways in the repression of Coffea TEs. These same NMD mechanisms can also regulate plant host gene expression via the repression of genes that bear exonized TE sequences. (Résumé d'auteur

T-Cell Memory Responses Elicited by Yellow Fever Vaccine are Targeted to Overlapping Epitopes Containing Multiple HLA-I and -II Binding Motifs

The yellow fever vaccines (YF-17D-204 and 17DD) are considered to be among the safest vaccines and the presence of neutralizing antibodies is correlated with protection, although other immune effector mechanisms are known to be involved. T-cell responses are known to play an important role modulating antibody production and the killing of infected cells. However, little is known about the repertoire of T-cell responses elicited by the YF-17DD vaccine in humans. In this report, a library of 653 partially overlapping 15-mer peptides covering the envelope (Env) and nonstructural (NS) proteins 1 to 5 of the vaccine was utilized to perform a comprehensive analysis of the virus-specific CD4+ and CD8+ T-cell responses. The T-cell responses were screened ex-vivo by IFN-γ ELISPOT assays using blood samples from 220 YF-17DD vaccinees collected two months to four years after immunization. Each peptide was tested in 75 to 208 separate individuals of the cohort. The screening identified sixteen immunodominant antigens that elicited activation of circulating memory T-cells in 10% to 33% of the individuals. Biochemical in-vitro binding assays and immunogenetic and immunogenicity studies indicated that each of the sixteen immunogenic 15-mer peptides contained two or more partially overlapping epitopes that could bind with high affinity to molecules of different HLAs. The prevalence of the immunogenicity of a peptide in the cohort was correlated with the diversity of HLA-II alleles that they could bind. These findings suggest that overlapping of HLA binding motifs within a peptide enhances its T-cell immunogenicity and the prevalence of the response in the population. In summary, the results suggests that in addition to factors of the innate immunity, "promiscuous" T-cell antigens might contribute to the high efficacy of the yellow fever vaccines. © 2013 de Melo et al