Estimation of temporal and spatial variations in groundwater recharge in unconfined sand aquifers using Scots pine inventories

Acknowledgements. This study was made possible through funding from the EU 7th Framework programme GENESIS (contract number 226536), AQVI project (no. 128377) in Academy of Finland AKVA research programme, the Renlund Foundation, VALUE doctoral school and Maa- ja vesitekniikan tuki ry. We would like to express our gratitude to Geological survey of Finland, Finnish Forest Administration (Metsähallitus) and Finnish Forest Centre (Metsäkeskus), Finnish meteorological institute, Finnish environmental administration and National land survey of Finland for providing data sets and expert knowledge that made this study possible in its current extent. To reproduce the research in the paper, data from above-mentioned agencies can be made available for purchase on request from the corresponding agency, other data can be provided by the corresponding author upon request. We thank Per-Erik Jansson for his assistance with the CoupModel and Jarkko Okkonen (GTK), anonymous reviewer, and Angelo Basile for their critical comments that significantly improved the manuscript.Peer reviewedPublisher PD

Using isotopes to constrain water flux and age estimates in snow-influenced catchments using the STARR (Spatially distributed Tracer-Aided Rainfall-Runoff) model

Acknowledgements. This work was funded by the NERC/JPI SIWA project (NE/M019896/1) and the European Research Council ERC (project GA 335910 VeWa). Numerical simulations were performed using the Maxwell High Performance Computing Cluster of the University of Aberdeen IT Service, provided by Dell Inc. and supported by Alces Software. The isotope work in Krycklan is funded by the KAW Branch-Point project together with SKB and SITES. We would like to thank Marjolein van Hui- jgevoort for her help with the STARR code, and Masaki Hayashi and two anonymous reviewers for their insightful suggestions that significantly improved the paper. The Supplement related to this article is available online at https://doi.org/10.5194/hess-21-5089-2017-supplement.Peer reviewedPublisher PD

Environmental tracers and indicators bringing together groundwater, surface water and groundwater-dependent ecosystems: importance of scale in choosing relevant tools

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Modeling the Isotopic Evolution of Snowpack and Snowmelt: Testing a Spatially Distributed Parsimonious Approach

Use of stable water isotopes has become increasingly popular in quantifying water flow paths and travel times in hydrological systems using tracer-aided modeling. In snow-influenced catchments, snowmelt produces a traceable isotopic signal, which differs from original snowfall isotopic composition because of isotopic fractionation in the snowpack. These fractionation processes in snow are relatively well understood, but representing their spatiotemporal variability in tracer-aided studies remains a challenge. We present a novel, parsimonious modeling method to account for the snowpack isotope fractionation and estimate isotope ratios in snowmelt water in a fully spatially distributed manner. Our model introduces two calibration parameters that alone account for the isotopic fractionation caused by sublimation from interception and ground snow storage, and snowmelt fractionation progressively enriching the snowmelt runoff. The isotope routines are linked to a generic process-based snow interception-accumulation-melt model facilitating simulation of spatially distributed snowmelt runoff. We use a synthetic modeling experiment to demonstrate the functionality of the model algorithms in different landscape locations and under different canopy characteristics. We also provide a proof-of-concept model test and successfully reproduce isotopic ratios in snowmelt runoff sampled with snowmelt lysimeters in two long-term experimental catchment with contrasting winter conditions. To our knowledge, the method is the first such tool to allow estimation of the spatially distributed nature of isotopic fractionation in snowpacks and the resulting isotope ratios in snowmelt runoff. The method can thus provide a useful tool for tracer-aided modeling to better understand the integrated nature of flow, mixing, and transport processes in snow-influenced catchments

Using stable isotopes to assess surface water source dynamics and hydrological connectivity in a high-latitude wetland and permafrost influenced landscape

The research has been supported by the NERC/JPI SIWA project (NE/M019896/1); grant issued in accordance with Resolution of the Government of the Russian Federation No. 220 dated 9 April 2010, under Agreement No. 14.B25.31.0001 with Ministry of Education and Science of the Russian Federation dated 24 June 2013 (BIO-GEO-CLIM); grant RFBR No 17-05-00-348a; grant FCP “Kolmogorov” 14.587.21.0036, grant RNF No 15-17-1009, and grant RFBR No 17-55-16008. Stable water isotope data are available in the Natural Environment Research Council (NERC) Environmental Information Data Centre (EIDC) data repository (title: “Stable water isotopes in Western Siberian inland waters”, permanent identifier: https://doi.org/10.5285/ca17e364-638d-4949-befb-b18b3770aec6). We would like to acknowledge the Arctic-GRO and IAEA for their publicly available databases providing supporting data for our analyses. Stream flow data at Nikolskoe was provided by Sergey Vorobiev. Liliya Kovaleva is acknowledged for the artwork in Figure 9. We would like to thank the two anonymous reviewers and the handling editors for their constructive comments that improved the manuscript.Peer reviewedPublisher PD

The value of scientific information on climate change: a choice experiment on Rokua esker, Finland

This article presents an application of the choice experiment method in order to provide estimates of economic values generated by water quantity improvements in the environment. More importantly, this is the first choice experiment study valuing scientific information and in particular scientific information on climate change. The case study of interest is Rokua in Northern Finland, a groundwater dependent ecosystem very sensitive to climate change and natural variability. The study deals with the uncertainty about the actual dynamics of the system and the effect of future climate change by exploring whether the public values sustained provision of resources for scientific research to better understand long-term environmental changes in Rokua. Data are analysed using a nested multinomial logit and an error component model. Evidence from this study suggests that individuals are willing to pay in order to assure scientific research so as to better understand long-term environmental changes. As a result, policy should consider investing in and supporting related research. Other aspects of water management policy valued by the public are water quantity, recreation, and total land income. We gratefully acknowledge the financial support from the European Union via the 7th Framework Program GENESIS: Groundwater and dependent ecosystems: New Scientific basis on climate change and land-use impact for the update of the EU Groundwater Directive; WP-6 Groundwater systems management: scenarios, risk assessment, cost-efficient measures and legal aspects. We finally thank two anonymous referees for constructive and insightful comments Koundouri, P.; Kougea, E.; Stithoua, M.; Ala-Ahob, P.; Eskelinenb, R.; Karjalainenc, T.; Klove, B.... (2012). The Value of Scientific Information on Climate Change: A Choice Experiment on Rokua esker, Finland. Journal of Environmental Economics and Policy. 1(1):85-102. doi:10.1080/21606544.2011.647450 Senia 85 102 1

Modeling the isotopic evolution of snowpack and snowmelt : Testing a spatially distributed parsimonious approach

This work was funded by the NERC/JPI SIWA project (NE/M019896/1) and the European Research Council ERC (project GA 335910 VeWa). The Krycklan part of this study was supported by grants from the Knut and Alice Wallenberg Foundation (Branch-points), Swedish Research Council (SITES), SKB and Kempe foundation. The data and model code is available upon request. Authors declare that they have no conflict of interest. We would like to thank the three anonymous reviewers for their constructive comments that improved the manuscript.Peer reviewedPublisher PD

High riverine CO2 emissions at the permafrost boundary of Western Siberia

Acknowledgements: The study was part of the JPI Climate initiative, financially supported by VR (the Swedish Research Council) grant no. 325-2014-6898 to J.K. Additional funding from RNF (RSCF) grant no. 18-17-00237, RFBR grant no. 17-55-16008 and RF Federal Target Program RFMEFI58717X0036 ‘Kolmogorov’ to O.S.P. and S.N.K. as well as NERC grant no. NE/M019896/1 to C.S. is acknowledged. The authors thank A. Sorochinskiy and A. Lim for assistance in the field, as well as M. Myrstener, M. Klaus and S. Monteux for advice on data analysis. L. Kovaleva is acknowledged for artwork.Peer reviewedPostprin

Keratinocyte Growth Factor Induces Gene Expression Signature Associated with Suppression of Malignant Phenotype of Cutaneous Squamous Carcinoma Cells

Keratinocyte growth factor (KGF, fibroblast growth factor-7) is a fibroblast-derived mitogen, which stimulates proliferation of epithelial cells. The expression of KGF by dermal fibroblasts is induced following injury and it promotes wound repair. However, the role of KGF in cutaneous carcinogenesis and cancer progression is not known. We have examined the role of KGF in progression of squamous cell carcinoma (SCC) of the skin. The expression of KGF receptor (KGFR) mRNA was lower in cutaneous SCCs (n = 6) than in normal skin samples (n = 6). Expression of KGFR mRNA was detected in 6 out of 8 cutaneous SCC cell lines and the levels were downregulated by 24-h treatment with KGF. KGF did not stimulate SCC cell proliferation, but it reduced invasion of SCC cells through collagen. Gene expression profiling of three cutaneous SCC cell lines treated with KGF for 24 h revealed a specific gene expression signature characterized by upregulation of a set of genes specifically downregulated in SCC cells compared to normal epidermal keratinocytes, including genes with tumor suppressing properties (SPRY4, DUSP4, DUSP6, LRIG1, PHLDA1). KGF also induced downregulation of a set of genes specifically upregulated in SCC cells compared to normal keratinocytes, including genes associated with tumor progression (MMP13, MATN2, CXCL10, and IGFBP3). Downregulation of MMP-13 and KGFR expression in SCC cells and HaCaT cells was mediated via ERK1/2. Activation of ERK1/2 in HaCaT cells and tumorigenic Ha-ras-transformed HaCaT cells resulted in downregulation of MMP-13 and KGFR expression. These results provide evidence, that KGF does not promote progression of cutaneous SCC, but rather suppresses the malignant phenotype of cutaneous SCC cells by regulating the expression of several genes differentially expressed in SCC cells, as compared to normal keratinocytes

Regulation of MMP-9 by p53 in first trimester cytotrophoblastic cells

BACKGROUND: The matrix metalloproteinase (MMP) family is known to play a key role in tissue remodelling during embryonic development and in pathological conditions, such as cardiovascular disease, arthritis and cancer metastasis. It has been shown previously that p53 regulates positively or negatively the expression of different MMPs. Because of p53 overexpression in trophoblastic cells, and its potential role in regulating MMP-2 and MMP-9 expression in different cell lines, we hypothesized that the expression of MMP-9 could also be regulated by p53 in first trimester cytotrophoblasts (CTB). METHODS and RESULTS: Transfection experiments in CTB demonstrated that wild-type p53 down-regulates the -670 (P < 0.001) but not the -531 and -90 human MMP-9 promoter/CAT reporter plasmid activity, whereas p53 mutants partially lost this repressive activity. However, endogenous p53 is not able to regulate MMP-9 expression in CTB. The presence of high molecular weight complexes of p53 in CTB suggests a potential mechanism of inactivation of p53 transcriptional activity towards MMPs in these cells. CONCLUSIONS: Although p53 is mutated in trophoblast, it is functionally incompetent towards MMPs in these cells