A molecular insight in sport and athletic alpha – actin3 gene in students of Physical Education Faculty and bodybuilders.

Alpha – actin3 is gene for athletic performance and sport. Three types of genotypes were identified within the gene: RR homozygous, RX heterozygous, and XX which lacks expression of these fibers. All three genotypes were identified among participants. Distribution of these genotypes was significant them, and it is noticeable that XX genotype was less in numbers than the other genotypes. BMI and BFP were in acceptable limits in athletes, but it was found to increase in control group with age. Two males and one female exhibited odd reading regarding muscle mass and testosterone level which was significantly higher than the others. Genetic analysis of these athletes showed SNPs that altered ORFs reading site, and production of different protein from the gene which affected muscle mass dramatically toward higher density, and triggered higher testosterone level in the body as an exceled response to intensive training. The three of them were found to be of XX genotype. Among athletic participants, physical fitness was high especially with young athletes. Most sprinters were found to be RR and RX genotype, while endurance athletes were of XX genotype. Significant SNPs at actin3 gene determined exceled athletes who were found to comprise less than 1%

Tumor necrosis factor receptor 2: its contribution to acute cellular rejection and clear cell renal carcinoma.

Tumor necrosis factor receptor 2 (TNFR2) is a type I transmembrane glycoprotein and one of the two receptors that orchestrate the complex biological functions of tumor necrosis factor (TNF, also designed TNF- α ). Accumulating experimental evidence suggests that TNFR2 plays an important role in renal disorders associated with acute cellular rejection and clear cell renal carcinoma but its exact role in these settings is still not completely understood. This papers reviews the factors that may mediate TNFR2 induction in acute cellular rejection and clear cell renal carcinoma and its contribution to these conditions and discusses its therapeutic implications. A greater understanding of the function of TNFR2 may lead to the development of new anti-TNF drugs.Peer Reviewe

Pro-environmental behavior and public understanding of climate change

The aim of this article is to examine whether awareness, knowledge and risk perception of climate change have significant influence on attitudes and pro-environmental behaviour. The study found that awareness, knowledge and risk perception of climate change positively influence the formation of favourable attitudes future action climate change. In addition, this paper also found mediated relationship through attitudes between awareness, knowledge, risk perception and pro-environmental behaviour. The paper argues that people are more likely to accept pro-environmental behaviours only if they have sufficient understanding of the adverse impacts of no action. This study offers decision makers field data to formulate relevant environmental policies and strategies in Malaysia

Citizens’ Perceptions of Corruption and E - Governance in Jordan, Ethiopia, and Fiji — the Need for a Marketing Approach

The purpose of this research is to assess citizen’s perceptions of corruption and e-governance in Jordan, Ethiopia, and Fiji. The research is based on surveys using structured questionnaires and focus group interviews. Conclusions are derived from a mix of descriptive and inferential analysis. The survey covers a total of 1212 respondents using stratified sampling. Findings reveal that public sector corruption and demands for bribes are increasing in each country. Only a few people are aware of e-governance and feel that it can help in curbing corruption. The study proposes that in order to mitigate negative forces in the implementation of e-governance such as corruption, digital divide, and urban bias, developing countries need to apply a marketing approach to e-governance services

Tumor necrosis factor receptor-2 signaling pathways promote survival of cancer stem-like CD133+ cells in clear cell renal carcinoma.

Clear cell renal cell carcinoma (ccRCC) contains cancer stem-like cells (CSCs) that express CD133 (ccRCC-CD133+). CSCs are rarely in cell cycle and, as nonproliferating cells, resist most chemotherapeutic agents. Previously, we reported that tumor necrosis factor receptor-2 (TNFR2) signaling promotes the cell cycle entry of ccRCC-CD133+CSCs, rendering them susceptible to cell-cycle-dependent chemotherapeutics. Here, we describe a TNFR2-activated signaling pathway in ccRCC-CD133+CSCs that is required for cell survival. Wild-type (wt)TNF or R2TNF but not R1TNF (TNF muteins that selectively bind to TNFR2 and TNFR1) induces phosphorylation of signal transducer and activator of transcription 3 (STAT3) on serine727 but not tyrosine705, resulting in pSTAT3Ser727 translocation to and colocalization with TNFR2 in mitochondria. R2TNF signaling activates a kinase cascade involving the phosphorylation of VEGFR2, PI-3K, Akt, and mTORC. Inhibition of any of the kinases or siRNA knockdown of TNFR2 or STAT3 promotes cell death associated with mitochondrial morphological changes, cytochrome c release, generation of reactive oxygen species, and TUNEL+cells expressing phosphorylated mixed lineage kinase-like (MLKL). Pretreatment with necrostatin-1 is more protective than z-VAD.fmk, suggesting that most death is necroptotic and TNFR2 signaling promotes cell survival by preventing mitochondrial-mediated necroptosis. These data suggest that a TNFR2 selective agonist may offer a potential therapeutic strategy for ccRCC

The Role of Asset Management, Operational Efficiency and Expense Management on the Performance of Commercial Banks in Bangladesh

The performance of the banks depends on some bank-specific factors. This paper set out to investigate the influence of asset management, operational efficiency and expense management on the financial performance of five commercial banks in Bangladesh for the period of 2011-2015. Descriptive statistics, correlation, and regression techniques were applied to find out the ultimate results. The empirical study suggested that operational efficiency had a positive effect on the dependent variables return on asset (ROA), and return on equity (ROE), but expense management was negatively related to the both indicators. On the other hand, asset management was positively related to ROA but negatively related to ROE. In addition, the regression results summed up that the changes in the performance of commercial banks could explain by bank-specific factors selected for the study

TNFR2 ligation in human T regulatory cells enhances IL2-induced cell proliferation through the non-canonical NF-κB pathway.

Human T regulatory cells (T regs) express high levels of TNF receptor 2 (TNFR2). Ligation of TNFR2 with TNF, which can recognise both TNFR1 and TNFR2, or with a TNFR2-selective binding molecule, DARPin 18 (D18) activates canonical NF-κB signalling, assessed by IκBα degradation, and the magnitude of the response correlates with the level of TNFR2 expression. RNA-seq analysis of TNF- or D18-treated human T regs revealed that TNFR2 ligation induces transcription of NFKB2 and RELB, encoding proteins that form the non-canonical NF-κB transcription factor. In combination with IL2, D18 treatment is specific for T regs in (1) stabilising NF-κB-inducing kinase protein, the activator of non-canonical NF-κB signalling, (2) inducing translocation of RelB from cytosol to nucleus, (3) increasing cell cycle entry, and (4) increasing cell numbers. However, the regulatory function of the expanded T regs is unaltered. Inhibition of RelB nuclear translocation blocks the proliferative response. We conclude that ligation of TNFR2 by D18 enhances IL2-induced T regs proliferation and expansion in cell number through the non-canonical NF-κB pathway

Trapping a silicon(I) radical with carbenes: a cationic cAAC-silicon(I) radical and an NHC-parent-silyliumylidene cation

The trapping of a silicon(I) radical with N-heterocyclic carbenes is described. The reaction of the cyclic (alkyl)(amino) carbene [cAACMe] (cAACMe=:C(CMe2)2(CH2)NAr, Ar=2,6-iPr2C6H3) with H2SiI2 in a 3:1 molar ratio in DME afforded a mixture of the separated ion pair [(cAACMe)2Si:.]+I− (1), which features a cationic cAAC–silicon(I) radical, and [cAACMe−H]+I−. In addition, the reaction of the NHC–iodosilicon(I) dimer [IAr(I)Si:]2 (IAr=:C{N(Ar)CH}2) with 4 equiv of IMe (:C{N(Me)CMe}2), which proceeded through the formation of a silicon(I) radical intermediate, afforded [(IMe)2SiH]+I− (2) comprising the first NHC–parent-silyliumylidene cation. Its further reaction with fluorobenzene afforded the CAr−H bond activation product [1-F-2-IMe-C6H4]+I− (3). The isolation of 2 and 3 confirmed the reaction mechanism for the formation of 1. Compounds 1–3 were analyzed by EPR and NMR spectroscopy, DFT calculations, and X-ray crystallography

Kloning Manusia

In the last few years, very rapid progress in the cloning technology and its development towards human cloning has become a hotly-debated issue. Cloning, which is the process of formation of a number of individuals with the same genetic structure, can be done by means of embryo-splitting method and nuclear transfer. Human cloning through the nuclear transfer method is directed towards two purposes, i.e. reproduction and therapy. The relatively new transgenic technology can be combined with the cloning technique to produce clones with new genes. However, pros and cons arise concerning the development of research on human cloning, particularly cloning for reproductive purposes. Therefore, there is need for a moratorium period before human cloning can be performed in order that solutions for all kinds of problems related to safety and ethics can be found

The Efficacy of Sunitinib Treatment of Renal Cancer Cells Is Associated with the Protein PHAX In Vitro

Anti-angiogenic agents, such as the multi-tyrosine kinase inhibitor sunitinib, are key first line therapies for metastatic clear cell renal cell carcinoma (ccRCC), but their mechanism of action is not fully understood. Here, we take steps towards validating a computational prediction based on differential transcriptome network analysis that phosphorylated adapter RNA export protein (PHAX) is associated with sunitinib drug treatment. The regulatory impact factor differential network algorithm run on patient tissue samples suggests PHAX is likely an important regulator through changes in genome-wide network connectivity. Immunofluorescence staining of patient tumours showed strong localisation of PHAX to the microvasculature consistent with the anti-angiogenic effect of sunitinib. In normal kidney tissue, PHAX protein abundance was low but increased with tumour grade (G1 vs. G3/4; p 0.01), consistent with a possible role in cancer progression. In organ culture, ccRCC cells had higher levels of PHAX protein expression than normal kidney cells, and sunitinib increased PHAX protein expression in a dose dependent manner (untreated vs. 100 µM; p 0.05). PHAX knockdown in a ccRCC organ culture model impacted the ability of sunitinib to cause cancer cell death (p 0.0001 untreated vs. treated), suggesting a role for PHAX in mediating the efficacy of sunitinib