Highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants (Ananas comosus L. Merr, var. MD2)

The low acclimatization efficiency of pineapple seedlings obtained by in vitro culture has been one of the main limitations to massively scale micropropagation protocols. Various factors may be affecting the successful establishment of in vitro plants to ex vitro conditions, related to the plant and/or the environment and the management of seedlings. The objective of this study was to establish an efficient micropropagation and acclimatization system for the MD2 variety, with a high in vitro multiplication rate and a high greenhouse and field survival of pineapple in vitro plants. In the multiplication phase, the axillary buds were isolated and placed on a semisolid medium and three induction treatments with different concentrations of BAP (1.0, 3.0, 5.0 mg/L) and ANA (2.0 mg/L), were evaluated. After the third subculture, multiple shoots were transferred to a temporary immersion system (BIOMINT). During acclimatization and nursery phases, six treatments with different substrate compositions wereevaluated. The induction treatment IT3 (BAP 5.0 mg/L and ANA 2.0 mg/L) was selected for a significantly high multiplication rate. AT4 treatment (Soil + Coconut fiber + perlite 1:1:1) showed the highest survival rate (95%) and allowed the obtaining of well-developed plants. This system constitutes a valuable technology to introduce in vitro plants to the pineapple production scheme on a commercial scale

Aerosol lidar intercomparison in the framework of SPALINET- the SPAnish LIdar NETwork: methodology and results

A group of eight Spanish lidars was formed in order to extend the European Aerosol Research Lidar Network-Advanced Sustainable Observation System (EARLINET-ASOS) project. This study presents intercomparisons at the hardware and software levels. Results of the system intercomparisons are based on range-square-corrected signals in cases where the lidars viewed the same atmospheres. Comparisons were also made for aerosol backscatter coefficients at 1064 nm (2 systems) and 532 nm (all systems), and for extinction coefficients at 532 nm (2 systems). In total, three field campaigns were carried out between 2006 and 2007. Comparisons were limited to the highest layer found before the free troposphere, i.e., either the atmospheric boundary layer or the aerosol layer just above it. Some groups did not pass the quality assurance criterion on the first attempt. Following modification and improvement to these systems, all systems met the quality criterion. The backscatter algorithm intercomparison consisted of processing lidar signal profiles simulated for two types of atmospheric conditions. Three stages with increasing knowledge of the input parameters were considered. The results showed that all algorithms work well when all inputs are known. They also showed the necessity to perform, when possible, additional measurements to attain better estimation of the lidar ratio, which is the most critical unknown in the elastic lidar inversion

Covid-19: la malinterpretación de los datos de la pandemia daña la confianza del público

Postprint (published version

Evaluation of the Spanish population coverage of a prospective HLA haplobank of induced pluripotent stem cells

Background: iPSC (induced pluripotent stem cells) banks of iPSC lines with homozygous HLA (human leukocyte antigen) haplotypes (haplobanks) are proposed as an affordable and off-the-shelf approach to allogeneic transplantation of iPSC derived cell therapies. Cord blood banks offer an extensive source of HLA-typed cells suitable for reprogramming to iPSC. Several initiatives worldwide have been undertaken to create national and international iPSC haplobanks that match a significant part of a population. Methods: To create an iPSC haplobank that serves the Spanish population (IPS-PANIA), we have searched the Spanish Bone Marrow Donor Registry (REDMO) to identify the most frequently estimated haplotypes. From the top ten donors identified, we estimated the population coverage using the criteria of zero mismatches in HLA-A, HLA-B, and HLA-DRB1 with different stringencies: high resolution, low resolution, and beneficial mismatch. Results: We have calculated that ten cord blood units from homozygous donors stored at the Spanish cord blood banks can provide HLA-A, HLA-B, and HLA-DRB1 matching for 28.23% of the population. Conclusion: We confirm the feasibility of using banked cord blood units to create an iPSC haplobank that will cover a significant percentage of the Spanish and international population for future advanced therapy replacement strategies

Experiencias deportivas, psicosociales y administrativas del club deportivo el Boca de Bello en el año 2019

Las organizaciones del deporte, en este caso, el club deportivo El Boca de Bello, no solo existen de acuerdo con una normatividad y un ejercicio propio de la actividad que promueven. Hay otros campos y/o ciencias sociales, de la salud y hasta administrativas, que complementan y enriquecen la actividad que desarrollan. Son estas las razones que motivaron, precisamente a varios grupos de investigación y a una institución del deporte, a desarrollar un proyecto de investigación, que diera cuenta del aporte inter y multidisciplinario de algunas ciencias para el funcionamiento exitoso de una entidad del sector deportivo, en este caso, un club de fútbol del municipio de Bello (Antioquia). Gestas, grupo de investigación del Politécnico Colombiano Jaime Isaza Cadavid, se encargó de diagnosticar los aspectos administrativos y de gestión del club; Observatos (Tecnológico de Antioquia) y PSIDE (Universidad Católica Luis Amigó), sistematizaron los temas psicosociales, mientras que los profesionales e investigadores del mismo club deportivo El Boca, desarrollaron los aspectos deportivos relacionados con la preparación física, la táctica y estrategia de esa organización. Los resultados obtenidos dieron cuenta de la realidad deportiva, administrativa y psicosocial del club, planteando las debilidades y fortalezas, pero también las oportunidades de mejora que tiene, alejando, de paso, las amenazas que existen en un contexto social, en donde los deportistas tienen bastantes necesidades y muchas problemáticas cotidianas a resolver. Al final, la investigación que acá se socializa, presenta sus conclusiones y recomendaciones, las mismas que podrán ser aprovechas por los clubes y organizaciones deportivas del departamento y del país, en la búsqueda de un trabajo de excelente que los lleve a tener mejores resultados, ubicando sus actividades del lado de la calidad de vida, desarrollo humano y bienestar para la población. Es el aporte científico de tres grupos de investigación y un club deportivo, para nuestra sociedad, en los campos del deporte, la gestión y la psicología.Sports organizations, in this case, the El Boca de Bello sports club, not only exist in accordance with regulations and an exercise proper to the activity they promote. There are other fields and/or social, health and even administrative sciences that complement and enrich the activity they carry out. These are the reasons that motivated, precisely, several research groups and a sports institution, to develop a research project, which would account for the inter and multidisciplinary contribution of some sciences for the successful operation of an entity in the sports sector, in this case, a football club in the municipality of Bello (Antioquia). Gestas, a research group from the Colombian Polytechnic Jaime Isaza Cadavid, was in charge of diagnosing the administrative and management aspects of the club; Observatos (Tecnológico de Antioquia) and PSIDE (Universidad Católica Luis Amigó) systematized psychosocial issues, while professionals and researchers from the same El Boca sports club developed sports aspects related to physical preparation, tactics and strategy of that organization. The results obtained gave an account of the sporting, administrative and psychosocial reality of the club, raising the weaknesses and strengths, but also the opportunities for improvement that it has, moving away, incidentally, the threats that exist in a social context, where athletes have many needs and many daily problems to solve.Politécnico Colombiano Jaime Isaza CadavidCONTENIDO Autores................................................................................................................... 5 Prólogo ................................................................................................................... 9 Introducción ........................................................................................................ 11 La problemática deportiva, administrativa y psicosocial, en el Club Deportivo El Boca Bello .................................................................... 13 Los objetivos propuestos....................................................................................... 14 Los soportes teóricos y legales ............................................................................. 14 Antecedentes......................................................................................................... 14 Desde lo legal ....................................................................................................... 28 Bases legales......................................................................................................... 29 Club deportivo ...................................................................................................... 32 Las bases teóricas.................................................................................................. 33 Psicología.............................................................................................................. 33 Variables psicológicas – motivación..................................................................... 44 Comunicación ....................................................................................................... 45 Cohesión grupal .................................................................................................... 45 Autoconfianza ....................................................................................................... 45 Tolerancia a la frustración..................................................................................... 46 Atención ............................................................................................................... 46 Concentración ....................................................................................................... 46 Psicología comunitaria.......................................................................................... 47 Psicosocial............................................................................................................. 48 Diferencial............................................................................................................. 49 Curso de vida ........................................................................................................ 49 Fútbol.................................................................................................................... 49 El deporte del fútbol.............................................................................................. 49 Preparación física (Estructura condicional).......................................................... 50 Cineantropometría ................................................................................................ 50 Test de Luc Leger.................................................................................................. 54 Estructura bioenergética ....................................................................................... 61 Estructura cognitiva ............................................................................................. 61 Estructura coordinativa: ....................................................................................... 61 Estructura condicional: ........................................................................................ 62 Estructura creativa: ............................................................................................... 62 Estructura socio – afectiva:................................................................................... 62 Estructura emotiva – volitiva:............................................................................... 62 Microciclos: .......................................................................................................... 63 Gestión deportiva .................................................................................................. 64 Mercadeo en el deporte......................................................................................... 73 Comunicación estratégica en el deporte ............................................................... 75 Club Deportivo El Boca........................................................................................ 76 Objetivos específicos: ........................................................................................... 79 Club Deportivo El Boca (2021) sostenimiento y presupuesto.............................. 79 El recorrido metodológico ................................................................................... 80 Los resultados y el análisis de la información ...................................................... 81 Conocer las estrategias de gestión deportiva del Club.......................................... 81 Estudiar las acciones y prácticas desarrollados en el componente ...................... 88 psicosocial............................................................................................................. 88 Acciones psicológicas y líneas metodológicas en el Club Deportivo .................. 88 El Boca ................................................................................................................. 88 Retos, dificultades y experiencias significativas del proceso................................ 95 Caracterización del Club Deportivo El Boca Bello .............................................. 96 Un ejercicio desde la psicología del deporte....................................................... 109 Descripción de los procesos de entrenamiento y competencias ........................ 116 de la institución en el período planteado............................................................. 116 Proceso de convocatoria ..................................................................................... 117 Acondicionamiento y evaluaciones .................................................................... 118 La planificación en el Club Boca Bello .............................................................. 120 Pretemporada ...................................................................................................... 120 Temporada........................................................................................................... 124 Deserción ............................................................................................................ 130 Jugadores que pasan a equipos profesionales..................................................... 130 Conclusiones ...................................................................................................... 131 Recomendaciones ............................................................................................... 134 Referencias.......................................................................................................... 136 Anexos................................................................................................................ 14

Study on aerosol properties over Madrid (Spain) by multiple instrumentation during SPALI10 lidar campaign

Understanding the effect of aerosols upon radiative forcing requ ires information about vertical profiles. Lidar techniques represent a powerful tool for studies of the vertical structure of the aerosol field. During the SPAin Lidar Intercomparison 20 10 (SPALI10) campaign, several multiwavelen gth Raman lidar systems measured simultaneously in order to assess their performances. Multiwavelength lidars can pr ovide relevant vertically-resolved information on aerosol optical properties because the wavelength dependence of the backscatter and extinction coefficients allows for a more detailed discrimination of aerosol types. Several lidar stations belonging to SPALINET, the Spanish and Portuguese Lidar NETwork and also EARLINET, the European Aerosol Research Lidar NETwork, intercompared during a campaign that took place in Madrid from 18 October to 5 November 2010. The products provided by the lidar systems were compared with ancillary data. At ground level, aerosol size distribution was continuously monitored. Additionally, the column-integrated characterization of the atmospheric aerosol was performed by means of a sun photometer. The extensive dataset obtained during SPALI10 field campaign enables to compare ground-level in-situ measurements with remote sensing techniques to determine vertically-resolved optical and microphysical properties of aerosols. Several relevant features shown in the comparison of the results obtained by the different instruments are discussed in this work.Peer ReviewedPostprint (published version

EARLINET instrument intercomparison campaigns: Overview on strategy and results

This paper introduces the recent European Aerosol Research Lidar Network (EARLINET) quality-assurance efforts at instrument level. Within two dedicated campaigns and five single-site intercomparison activities, 21 EARLINET systems from 18 EARLINET stations were intercompared between 2009 and 2013. A comprehensive strategy for campaign setup and data evaluation has been established. Eleven systems from nine EARLINET stations participated in the EARLINET Lidar Intercomparison 2009 (EARLI09). In this campaign, three reference systems were qualified which served as traveling standards thereafter. EARLINET systems from nine other stations have been compared against these reference systems since 2009. We present and discuss comparisons at signal and at product level from all campaigns for more than 100 individual measurement channels at the wavelengths of 355, 387, 532, and 607 nm. It is shown that in most cases, a very good agreement of the compared systems with the respective reference is obtained. Mean signal deviations in predefined height ranges are typically below ±2 %. Particle backscatter and extinction coefficients agree within ±2  ×  10−4 km−1 sr−1 and ± 0.01 km−1, respectively, in most cases. For systems or channels that showed larger discrepancies, an in-depth analysis of deficiencies was performed and technical solutions and upgrades were proposed and realized. The intercomparisons have reinforced confidence in the EARLINET data quality and allowed us to draw conclusions on necessary system improvements for some instruments and to identify major challenges that need to be tackled in the future

Covid-19: la malinterpretación de los datos de la pandemia daña la confianza del público

Dos cartas publicadas en The Lancet y The Lancet Public Health en los últimos meses defienden la necesidad de evaluar de forma independiente la respuesta española ante la covid-19. Estamos de acuerdo, pero nos gustaría complementarlas con tres puntos que nos acercan a la ciencia abierta. Nos referimos a la confusión terminológica, la calidad de los datos y su disponibilidad

Evolving trends in the management of acute appendicitis during COVID-19 waves. The ACIE appy II study

Background: In 2020, ACIE Appy study showed that COVID-19 pandemic heavily affected the management of patients with acute appendicitis (AA) worldwide, with an increased rate of non-operative management (NOM) strategies and a trend toward open surgery due to concern of virus transmission by laparoscopy and controversial recommendations on this issue. The aim of this study was to survey again the same group of surgeons to assess if any difference in management attitudes of AA had occurred in the later stages of the outbreak. Methods: From August 15 to September 30, 2021, an online questionnaire was sent to all 709 participants of the ACIE Appy study. The questionnaire included questions on personal protective equipment (PPE), local policies and screening for SARS-CoV-2 infection, NOM, surgical approach and disease presentations in 2021. The results were compared with the results from the previous study. Results: A total of 476 answers were collected (response rate 67.1%). Screening policies were significatively improved with most patients screened regardless of symptoms (89.5% vs. 37.4%) with PCR and antigenic test as the preferred test (74.1% vs. 26.3%). More patients tested positive before surgery and commercial systems were the preferred ones to filter smoke plumes during laparoscopy. Laparoscopic appendicectomy was the first option in the treatment of AA, with a declined use of NOM. Conclusion: Management of AA has improved in the last waves of pandemic. Increased evidence regarding SARS-COV-2 infection along with a timely healthcare systems response has been translated into tailored attitudes and a better care for patients with AA worldwide

Liver trauma: WSES 2020 guidelines

Abstract: Liver injuries represent one of the most frequent life-threatening injuries in trauma patients. In determining the optimal management strategy, the anatomic injury, the hemodynamic status, and the associated injuries should be taken into consideration. Liver trauma approach may require non-operative or operative management with the intent to restore the homeostasis and the normal physiology. The management of liver trauma should be multidisciplinary including trauma surgeons, interventional radiologists, and emergency and ICU physicians. The aim of this paper is to present the World Society of Emergency Surgery (WSES) liver trauma management guidelines