Quantification of respiratory parameters in patients with temporal lobe epilepsy

Dysfunction affecting cardiac or pulmonary systems has been postulated as a major factor in sudden death in epilepsy (SUDEP). Whilst the majority of studies of cardiorespiratory function have focused on changes during seizures, here we investigate whether epilepsy influences basal respiratory parameters in patients with temporal lobe epilepsy (TLE) during the interictal period. Spirometry was performed in 10 females and 10 males. Measurements of Vital Capacity (VC), Forced Vital Capacity (FVC), Forced Expiratory Volume in the first second (FEV1) and ratios of FEV1 to FVC (FEV1/FVC) were obtained, and these values were analyzed as percentages of predicted values. None of the patients had chronic obstructive pulmonary disease and no significant alterations in respiratory function tests were found among these patients. No association between seizure frequency, antiepileptic drugs and SUDEP could be found in this study. Although the study did not identify any specific respiratory abnormality in TLE patients during the interictal period, re-evaluation of clinical data on pulmonary disorders in people with epilepsy should be better investigated

TELESSAÚDE: NOVOS CAMINHOS NA ATENÇÃO À SAÚDE FRENTE À INFECÇÃO PELO NOVO CORONAVÍRUS

A infecção pelo novo coronavírus tem como uma de suas principais medidas preventivas o distanciamento entre as pessoas. Essa medida de proteção à saúde e à vida favoreceu a implantação e/ou implementação da Telessaúde no Brasil que tem como premissa a melhoria da rede de serviços de saúde, sobretudo da Atenção Primária à Saúde (APS) a partir da teleconsultoria, telediagnóstico, telemonitoramento, telerregulação e teleducação. Aqui, o objetivo é apontar novos caminhos na atenção à saúde frente à infecção pelo novo coranavírus a partir da telessaúde. A Telessaúde no Paraná se estabeleceu com o advento da infecção pelo novo coronavírus e se efetivou a partir de projeto de extensão de prevenção e combate ao coronavirus que envolveu Sesa, Seti e IEES do estado. Desta forma, trata-se de relato de experiência de alunos bolsista desse projeto. A Telessáude já beneficiou mais de 1 milhão de pessoas no Brasil e mais de 8 mil pessoas no Paraná com as ações de teleconsultoria telediagnóstico, telemonitoramento e teleeducação no enfrentamento ao novo coronavirus. A Telessaúde tem o potencial para melhorar os serviços de saúde no pós-pandemia, pois tem se mostrado resolutiva, humanizada, garantindo baixo custo, acessibilidade e redução de filas nos serviços de saúde

Reciprocating and rotatory NiTi instruments used for root canal preparation of primary teeth: a systematic review and meta-analysis

Objective: To compare the root canal preparation of primary teeth with reciprocating and rotary NiTi instruments. Material and Methods: Electronic databases (PubMed/MEDLINE, Web of Science, TRIP, Lilacs, Embase, and Scopus) were systematically searched until October 2020. In vitro studies comparing the cleaning ability, debris extrusion, file deformation, or working time of rotary and reciprocating NiTi instruments in primary teeth were evaluated. Two reviewers independently selected the studies, extracted the data, and assessed the risk of bias. Meta-analyses were conducted using a random-effects model to calculate pooled mean differences between reciprocating and rotary NiTi instruments considering the outcomes: working time (minutes) and debris extrusion (milligrams). Statistical analyses were performed using RevMan 5.3 at a significance level of 5%. Results: From 4,417 potentially relevant studies, 10 were included in the systematic review, and 8 considered in the meta-analyses. There was no significant difference between reciprocating and rotary NiTi instruments considering debris extrusion [3 data sets; effect size: -0.11 (-0.25-0.04); p=0.15] and working time [6 data sets; effect size: -0.37 (-0.98-0.24); p=0.24]. The heterogeneity found was moderate to high. The risk of bias was low in most studies (50.0% of all items across studies). Conclusion: There is no scientific evidence showing superiority of reciprocating or rotary NiTi instruments used for root canal preparation in primary teeth

Reciprocating and Rotatory NiTi Instruments Used for Root Canal Preparation of Primary Teeth: A Systematic Review and Meta-Analysis

Objective: To compare the root canal preparation of primary teeth with reciprocating and rotary NiTi instruments. Material and Methods: Electronic databases (PubMed/MEDLINE, Web of Science, TRIP, Lilacs, Embase, and Scopus) were systematically searched until October 2020. In vitro studies comparing the cleaning ability, debris extrusion, file deformation, or working time of rotary and reciprocating NiTi instruments in primary teeth were evaluated. Two reviewers independently selected the studies, extracted the data, and assessed the risk of bias. Meta-analyses were conducted using a random-effects model to calculate pooled mean differences between reciprocating and rotary NiTi instruments considering the outcomes: working time (minutes) and debris extrusion (milligrams). Statistical analyses were performed using RevMan 5.3 at a significance level of 5%. Results: From 4,417 potentially relevant studies, 10 were included in the systematic review, and 8 considered in the meta-analyses. There was no significant difference between reciprocating and rotary NiTi instruments considering debris extrusion [3 data sets; effect size: -0.11 (-0.25–0.04); p=0.15] and working time [6 data sets; effect size: -0.37 (-0.98–0.24); p=0.24]. The heterogeneity found was moderate to high. The risk of bias was low in most studies (50.0% of all items across studies). Conclusion: There is no scientific evidence showing superiority of reciprocating or rotary NiTi instruments used for root canal preparation in primary teeth

Quantificação de parâmetros respiratórios em pacientes com epilepsia do lobo temporal

Dysfunction affecting cardiac or pulmonary systems has been postulated as a major factor in sudden death in epilepsy (SUDEP). Whilst the majority of studies of cardiorespiratory function have focused on changes during seizures, here we investigate whether epilepsy influences basal respiratory parameters in patients with temporal lobe epilepsy (TLE) during the interictal period. Spirometry was performed in 10 females and 10 males. Measurements of Vital Capacity (VC), Forced Vital Capacity (FVC), Forced Expiratory Volume in the first second (FEV1) and ratios of FEV1 to FVC (FEV1/FVC) were obtained, and these values were analyzed as percentages of predicted values. None of the patients had chronic obstructive pulmonary disease and no significant alterations in respiratory function tests were found among these patients. No association between seizure frequency, antiepileptic drugs and SUDEP could be found in this study. Although the study did not identify any specific respiratory abnormality in TLE patients during the interictal period, re-evaluation of clinical data on pulmonary disorders in people with epilepsy should be better investigated.Alterações cardíacas e pulmonares têm sido propostas como principais fatores de risco no fenômeno da morte súbita em epilepsia (SUDEP). Enquanto a maioria dos estudos tem verificado as alterações da função cardiorrespiratória durante as crises epilépticas, nosso estudo avaliou a função pulmonar de indivíduos com epilepsia do lobo temporal (ELT) no período interictal. Vinte pacientes com ELT (10 homens e 10 mulheres) participaram de nosso estudo. Nos testes de função pulmonar foram mensurados os seguintes parâmetros: Capacidade Vital Forçada (CVF), Capacidade Vital Lenta (CVL), Volume Expiratório Forçado no primeiro segundo (VEF1) e o Índice VEF1/CVF. Os resultados foram comparados aos valores de normalidade preditos na literatura. Nenhum dos pacientes apresentou doença pulmonar obstrutiva ou restritiva e alterações significativas nos testes de função pulmonar. Não foram encontradas associações entre a freqüência de crises, drogas antiepilépticas e SUDEP. Embora nosso estudo não tenha identificado qualquer anormalidade respiratória específica em indivíduos com ELT durante o período interictal, os dados clínicos sobre transtornos pulmonares em indivíduos com epilepsia devem ser melhor avaliados.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Laboratório de Neurologia ExperimentalUniversidade de Mogi das Cruzes Núcleo de Pesquisas Tecnológicas Laboratório de NeurociênciasUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de FisiologiaUniversity College of London Institute of Neurology Department of Clinical and Experimental EpilepsyUNIFESP, EPM, Laboratório de Neurologia ExperimentalUNIFESP, EPM, Depto. de FisiologiaSciEL

Determinação da ingestão de macro e micro nutrientes na dieta de nadadores fundistas masteres

Para que atletas tenham uma dieta adequada e satisfatória na quantidade e qualidade alimentar, os mesmos devem contemplar em equilíbrio os percentuais de macro e micronutrientes, a fim de obterem uma melhora do seu desempenho físico durante os treinos e competições. A presente pesquisa teve por objetivo determinar a ingesta dietética de macro e micronutrientes em nadadores fundistas másters. Fizeram parte da amostra 10 atletas do sexo masculino, com idade mediana de 36,5 anos estatura e peso. Para obtenção das variáveis nutricionais, foi utilizado o inquérito alimentar de três dias, independente do fator dia de treino. Com os dados obtidos, os atletas apresentaram valores de 90% abaixo do recomendado para carboidratos, 80% acima do recomendado para proteínas, e 90% acima do recomendado para lipídeo, pode-se concluir que os atletas avaliados possuíam um perfil dietético para macronutrientes, hiperproteico, hipolipídico, hipoglicídico. Em relação aos micronutrientes os atletas avaliados apresentaram-se abaixo das recomendações para vitamina A, cálcio, magnésio e potássio, equilibrada em vitamina B2 e acima vitamina B1, B6, B12 e sódio. E com uma ingesta dietética de micronutrientes abaixo do recomendado, os atletas podem acabar por diminuir o seu desempenho durante a competição, pois os mesmos fornecem energia para ser utilizada durante a prática do exercício. ABSTRACTDetermination the ingestion of macro and micronutrients in the diet of runners masters swimeersSo that athletes have a proper and satisfactory diet in quantity and quality food, the same shall contemplate on balance the percentages in macro and micronutrients, in order to obtain an improvement in their physical performance during training and competitions. The present research aimed to determine the dietary intake of macro and micronutrients in runners Masters Swimmers. The sample included 10 male athletes, with a median age of 36.5 years of height and weight. To obtain the nutritional variables, It was used nutritional survey than three days, regardless the factor of the training day. With the data obtained, the athletes presented values of 90% lower than recommended for carbohydrates, 80% higher than recommended for proteins, and 90% higher than recommended for lipids, can conclude that the evaluated athletes had a dietary profile for macronutrients, High in protein, hypolipidic and low in carbohydrates. Regarding micronutrients, the athletes evaluated presented lower of the recommendations for vitamin A, Calcium, magnesium and Potassium, balanced vitamin B2 and higher vitamin B1, B6, B12 and Sodium. And with a dietary intake of macronutrients lower than recommended, the athletes can end up decreasing their performance during the competition, therefore, these same provide energy to be used during the practice of exercise

A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells

Background: Mesenchymal stromal cells (MSCs) are multipotent progenitor cells used in several cell therapies. MSCs are characterized by the expression of CD73, CD90, and CD105 cell markers, and the absence of CD34, CD45, CD11a, CD19, and HLA-DR cell markers. CD90 is a glycoprotein present in the MSC membranes and also in adult cells and cancer stem cells. The role of CD90 in MSCs remains unknown. Here, we sought to analyse the role that CD90 plays in the characteristic properties of in vitro expanded human MSCs. Methods: We investigated the function of CD90 with regard to morphology, proliferation rate, suppression of T-cell proliferation, and osteogenic/adipogenic differentiation of MSCs by reducing the expression of this marker using CD90-target small hairpin RNA lentiviral vectors. Results: The present study shows that a reduction in CD90 expression enhances the osteogenic and adipogenic differentiation of MSCs in vitro and, unexpectedly, causes a decrease in CD44 and CD166 expression. Conclusion: Our study suggests that CD90 controls the differentiation of MSCs by acting as an obstacle in the pathway of differentiation commitment. This may be overcome in the presence of the correct differentiation stimuli, supporting the idea that CD90 level manipulation may lead to more efficient differentiation rates in vitro

Comparative study between two recombinant human NPH insulin formulations for the treatment of type 2 diabetes mellitus

ABSTRACT Objective: To compare the effects of the neutral protamine Hagedorn (NPH) recombinant human insulin formulations Gansulin and Humulin N ® on the glycemic control of patients with type 2 diabetes mellitus (T2DM). Subjects and methods: Prospective, double-blind, randomized, parallel, singlecenter study of 37 individuals with T2DM treated with NPH insulin formulations. The Tukey-Kramer test for multiple comparisons, the Wilcoxon paired comparison test and the Chi-Square test were used for the statistical analyses. The significance level was set at 5% (p < 0.05). Results: The NPH insulin formulations Humulin and Gansulin similarly reduced the HbA1c levels observed at the end of the study compared with the values obtained at the beginning of the study. In the Humulin group, the initial HbA1c value of 7.91% was reduced to 6.56% (p < 0.001), whereas in the Gansulin group, the reduction was from 8.18% to 6.65% (p < 0.001). At the end of the study, there was no significant difference between the levels of glycated hemoglobin (p = 0.2410), fasting plasma glucose (FG; p = 0.9257) and bedtime plasma glucose (BG; p = 0.3906) between the two insulin formulations. There was no nt diffe rence in the number of hypoglycemic events between the two insulin formulations, and no severe hyp episodes were recorded. Conclusion: This study demonstrated similar glycemic control by NPH insulin Gansulin compared with human insulin Humulin N ® in patients with T2DM

Ação de fertilizante orgânico de resíduos vegetais em condições de estresse abiótico no teor de fenólicos totais de alfaces (lactuca sativa) / Impact organic fertilizer produced with vegetable residues with stress abiotic condition on the total phenolics content of lettuce (lactuca sativa)

O estresse hídrico pode ser desejável por promover aumento da produção de fitoquímicos e bioativos antioxidantes, mas também impactam no tamanho das folhas, acúmulo de mucilago e outros metabólitos. Grande parte da produção de alimentos perdida, é representada por vegetais. Resíduos de frutas e hortaliças são ricos em fibras e bioativos antioxidantes. O objetivo deste estudo é avaliar a ação de resíduos vegetais (FFH e casca de cebola) no acondicionamento do solo em condições de estresse abiótico associado ao teor de compostos fenólicos totais. O experimento foi realizado na casa de vegetação do Centro de Ciências da Saúde (CCS) da Universidade Federal do Rio de Janeiro (UFRJ) no período de 25/03 a 04/09 de 2019. As análises do solo foram umidade (U), capacidade de retenção de água (CRa) e respiração basal (RBS); já nas folhas de alface foram analisadas altura, teor total de fenólicos e atividade antioxidante por FRAP. Todas as análises foram feitas em triplicada e no tratamento dos dados foram aplicadas análise de variância e teste de Tukey                (p< 0,05). O solo adicionado de FFH 3% apresentou boa resposta quanto aos parâmetros U, CRa e RBS, propiciando crescimento das folhas de alface em 86 dias, em contrapartida ao solo sem adição de fertilizante, 122 dias. Não houve crescimento no solo  com adição de FCC 10%. O solo com FFH 3% promoveu aumento significativo do teor de compostos fenólicos e a capacidade antioxidante das folhas de alface, mostrando alto potencial para aplicação da produção agrícola, visando melhora do perfil nutricional, bem como redução hídrica durante cultivo