A parentage study of closely related ukrainian wine grape varieties using microsatellite markers

International audienceFour bred grapevine varieties released for commercial cultivation in Ukraine, namely ‘Antey Magarachs_ kii’, ‘Rubinovyi Magaracha’, ‘Granatovyi Magaracha’ and ‘Rubin Golodrigi’, and their putative parental forms were genotyped using six microsatellite loci. Genotypes were compared with breeding records to verify genetic rela_ tionships among varieties. Results of the analysis confirmed four of six parent_offspring relationships. Results of the analysis allow to assume that genotype ‘Seyve Villard 20347’ is the direct parent of ‘Antey Magarachskii’ instead of its grandparent. The first_studied accession believed to be that of ‘Granatovyi Magaracha’ was identified as impurity.In order to verify the parentage of ‘Granatovyi Magaracha’, rest accessions of that variety and its putative parent ‘Antey Magarachskii’ were additionally genotyped at 13 nuclear loci and at three chloroplast loci. The parent_off_ spring relationship was confirmed, as all ‘Granatovyi Magaracha’ accessions had a common allele with the parent variety ‘Antey Magarachskii’ at each locus and the same chlorotype A. Different ‘Granatovyi Magaracha’ accessions could have been obtained via vegetative propagation of two seedlings which arose from one crossing

Multiple origins of cultivated grapevine (Vitis vinifera L. ssp. sativa) based on chloroplast DNA polymorphisms

R. Arroyo-García et al.The domestication of the Eurasian grape (Vitis vinifera ssp. sativa) from its wild ancestor (Vitis vinifera ssp. sylvestris) has long been claimed to have occurred in Transcaucasia where its greatest genetic diversity is found and where very early archaeological evidence, including grape pips and artefacts of a ‘wine culture’, have been excavated. Whether from Transcaucasia or the nearby Taurus or Zagros Mountains, it is hypothesized that this wine culture spread southwards and eventually westwards around the Mediterranean basin, together with the transplantation of cultivated grape cuttings. However, the existence of morphological differentiation between cultivars from eastern and western ends of the modern distribution of the Eurasian grape suggests the existence of different genetic contribution from local sylvestris populations or multilocal selection and domestication of sylvestris genotypes. To tackle this issue, we analysed chlorotype variation and distribution in 1201 samples of sylvestris and sativa genotypes from the whole area of the species’ distribution and studied their genetic relationships. The results suggest the existence of at least two important origins for the cultivated germplasm, one in the Near East and another in the western Mediterranean region, the latter of which gave rise to many of the current Western European cultivars. Indeed, over 70% of the Iberian Peninsula cultivars display chlorotypes that are only compatible with their having derived from western sylvestris populations.This research was supported by grants from the Comunidad de Madrid (grant 07G/0045/2000) to the Spanish CNB-CSIC research group, from INIA (RF02-010-C3-1) to the INIA, University of Sevilla and IMIDRA research groups and from the State Planning Organization of Turkey (project no: 2001-K-120–240) for Turkish research groups.Peer reviewe

Chloroplast microsatellites to investigate the origin of grapevine

The origin of the grapevine, Vitis vinifera L. (ssp.) sativa has been investigated with archaeobotanical–archaeological, cultural and historical data indicating a unique domestication centre located in the Caucasianand Middel-East regions about 6–7000 years ago, but, events leading to the domestication of thisspecies are still an open issue. In this work, eight universal chloroplast microsatellites are used to assessgenetic relationships among varieties selected as representatives of four distinct geographical groups fromMiddle-East to Western European regions. Results show that two out of the eight analysed chloroplast lociare polymorphic within the 142 individuals. Allele variants of the cpSSR loci combine in a total of sixdifferent haplotypes. The analysis of haplotypes distribution and haplotype diversity (HD) suggest that onlythree out of the six haplotypes are represented in the Caucasian and Middle-East samples, with 90% ofindividuals sharing the same haplotype. Moreover, the presence of all six haplotypes in the Europeanaccessions, with a high level of haplotype diversity, suggests varietal influx in these areas. Concerning theWestern European varieties, especially in Spanish accessions, half of the individuals share haplotype VIwhich is completely absent in the Caucasian and Middle-East cultivars. This result opens the discussionabout the existence of a unique and common domestication centre, located in the Caucasian and Middle-East area, for all the European cultivars.This work suggests the usefulness of chloroplast genome markers to provide information on haplotypedistributions that could help to identify further geographical areas for grapevine varietal evolution

Genetic diversity in table grapes based on RAPD and microsatellite markers

The objective of this work was to analyze the genetic diversity of 47 table grape accessions, from the grapevine germplasm bank of Embrapa Semiárido, using 20 RAPD and seven microsatellite markers. Genetic distances between pairs of accessions were obtained based on Jaccard's similarity index for RAPD data and on the arithmetic complement of the weighted index for microsatellite data. The groups were formed according to the Tocher's cluster analysis and to the unweighted pair‑group method with arithmetic mean (UPGMA). The microsatellite markers were more efficient than the RAPD ones in the identification of genetic relationships. Information on the genetic distance, based on molecular characteristics and coupled with the cultivar agronomic performance, allowed for the recommendation of parents for crossings, in order to obtain superior hybrids in segregating populations for the table grape breeding program of Embrapa Semiárido