Shear strengthening of continuous reinforced concrete T-beams using deep embedment technique

Despite numerous studies, shear behaviour before or after strengthening is still not fully understood, particularly in continuous concrete structures which are the norm. Upgrading shear resistance is altogether more difficult since Externally Bonded Reinforcement (EBR) or Near Surface Mounted (NSM) techniques do not allow the FRP material to be anchored into the compression zone of the T-beams and they cannot be used in cases where the sides of the beams are inaccessible. An innovative retrofit technique, named Deep Embedment (DE) or Embedded Through Section (ETS) technique involves the insertion of FRP/steel bars upwards into vertical or inclined holes which have been drilled from the soffit of concrete beams. In this way, the tension and compression chords of the beams are directly connected while the bars are bonded to the concrete core through adhesives. With this technique strengthening can be done in cases where the webs are inaccessible. Thus the main focus of this study is to significantly contribute to the current knowledge on the behaviour of Reinforced Concrete continuous T-beamsstrengthened in shear using the DE technique where large shear forces are combined with large negative bending moments. An experimental program consisting of ten two-span continuous T-beams designed to fail in shear wascarried out in order to significantly contribute to the current knowledge on the behaviour of RC continuous Tbeams strengthened in shear using this technique. Therefore, this paper reports on the test results and on their significance in being able to apply this technique on concrete structures by validating them through adequate analytical models

Mechanics of failure in FRP strengthened reinforced concrete in shear

Carbon fibre reinforced polymer (CFRP) composite sheets and bars were used to strengthen reinforced concrete across a known plane to resist shear friction. The uncracked push-off specimens were either externally reinforced with CFRP sheets with a reinforcement ratio of 0.8% and 1.6% or internally with CFRP bars as additional shear reinforcement corresponding to 0.8% reinforcement ratio. Two ratios of internal steel reinforcement were considered representing the nominal stirrup reinforcement according to both historic (0.17%) and current design codes (0.26%). The effects of varying anchorage length of the CFRP on the shear friction capacity of the push-off specimens with externally bonded reinforcement (EBR) were studied through various strengthening schemes. Experimental results showed an increase in shear strength ranging from 23 – 84% compared to the unstrengthened control specimens. Tests with various wrapping schemes showed no evidence that additional shear friction capacity can be developed when beyond a sufficient anchorage length. However, specimens with short anchorage length failed prematurely due to the early debonding of the CFRP. The shear friction strength of the initially uncracked push- off specimens was determined using experimental results combining the shear friction contribution of the individual structural components, extending the currently accepted approach for internal steel reinforcement

Shear design of circular concrete sections using the eurocode 2 truss model

The introduction of the Eurocodes for concrete design will alter the way that shear is approached for concrete structures. BS EN 1992-1-1 has adopted the variable angle truss model for shear, a more theoretically consistent approach than that used in BS 8110-1. The model is confidently applied to rectangular sections, but its applicability to irregular sections is less clear. In particular, the behaviour of circular concrete sections is not well defined. This paper is intended to satisfy a requirement for design guidance on this topic that has been recognised by key BSI Committees. Using both experimental and theoretical data, the Eurocode variable angle truss model for shear design is assessed and extended to circular columns

Concrete structures using fabric formwork

Using fabric formwork, it is possible to cast architecturally interesting, optimised structures that use up to 40% less concrete than an equivalent strength prismatic section, thereby offering the potential for significant embodied energy savings in new concrete structures. This paper reports on the philosophy of and background to fabric formwork before techniques for the design, optimisation and shape prediction of fabric formed concrete beams are presented. The practicality of construction with non-orthogonal elements is discussed before the results of new structural test data, undertaken at the University of Bath on 4m span ’T’ beam elements formed in reusable fabric moulds, are presented. Potential areas of future development for fabric formwork, including the use of woven advanced composite fabrics as permanent participating formwork and the feasibility of uniform strength prestressed beams, are then discussed The practicality of construction with non-orthogonal elements is discussed before the results of new structural test data, undertaken at the University of Bath on 4m span ‘T’ beam elements formed in reusable fabric moulds, are presented. Potential areas of future development for fabric formwork, including the use of woven advanced composite fabrics as permanent participating formwork and the feasibility of uniform strength prestressed beams, are then discussed

Experimental Investigation of Reinforced Concrete T-Beams Strengthened in Shear with Externally Bonded CFRP Sheets

An experimental investigation was undertaken into the effectiveness of unanchored and anchored externally bonded (EB) U-wrapped carbon fibre reinforced polymer (CFRP) shear strengthening for reinforced concrete T-beams at a range of realistic sizes. The T-beam sizes, geometry and reinforcement were chosen to reflect existing slab-on-beam structures with low levels of transverse steel shear reinforcement. Geometrically similar reinforced concrete T-beams were tested across three sizes ranging from 360 to 720 mm in depth and with different amounts of EB CFRP shear reinforcement. The beams were subjected to three-point bending with a span to depth ratio of 3.5. All the beams failed in diagonal shear. The experimental results indicate significant variability in the capacity of unstrengthened control beams, and a number of these control beams showed greater shear capacity than their EB CFRP strengthened counterparts. Greater thicknesses of CFRP reinforcement did not lead to increased shear capacity compared with lesser thicknesses of unanchored or anchored EB CFRP, but anchored EB CFRP did lead to moderate increases in shear capacity compared to both control and unanchored EB CFRP strengthened beams.The authors gratefully acknowledge the help of the laboratory staff of University of Bath and University of Cambridge. The authors would also like to acknowledge the financial support of: the UK Engineering and Physical Sciences Research Council (under grants EPSRC EP/I018921/1 and EP/I018972/1); the Universities of Bath and Cambridge; and the project partners and sponsors – Parsons Brinckerhoff, Tony Gee and Partners LLP, Arup, Highways England, Concrete Repairs Ltd, LG Mouchel and Partners, The Concrete Society, Fyfe Europe S.A., Fibrwrap UK, Hughes Brothers and Ebor Concrete Ltd.This is the author accepted manuscript. It is currently under an indefinite embargo pending publication by ASCE

Time-dependent appearance of myofibroblasts in granulation tissue of human skin wounds

Human skin wounds (66) inflicted between 20 h and 7 months prior to biopsy were studied. In order to identify the type of cellular differentiation of the fibroblastic cells in the granulation tissue, alpha-smooth muscle actin and desmin were immunohistochemically localized. The value of any presumed time-dependent appearance and/or disappearance of positively stained cells was tested for the estimation of wound age. In skin specimens with a wound age less than 5 days (n =15) no typical granulation tissue had developed and no alpha-actin-positive myofibroblasts could be detected. The first appearance of positively reacting myofibroblasts was noted in a 5-day-old wound. In 57% of the lesions with a wound age between 5 and 31 days (25 out of 44 cases) typical granulation tissue formation was present and myofibroblasts with positive reaction for alpha-smooth muscle actin could be identified. Numerous positively reacting cells could generally be found in wounds aged between 16 and 31 days, but also in wounds less than 16 days old. In 29% of the cases with a wound age of more than 31 days (2 out of 7 cases) alpha-sma-positive myofibroblasts also occured. Fibroblastic cells positive for desmin could not be seen at all in our series. Our results demonstrate the appearance of alpha-sma-positive myofibroblasts with the initial formation of typical granulation tissue in human skin lesions as early as approximately 5 days after wounding. In contrast to recent experimental results these cells remained detectable in wounds aged more than 2 months in some cases. The immunohistochemical detection of actin-positive cells, therefore, demonstrates whether an unknown skin wound is aged approximately 5 days or more. Even though a time-dependent decrease of myofibroblasts in human granulation tissue after 31 days in human wounds seems probable, the extended presence (up to about 2 months) of these cells allows no further exact age determination of older wounds

Caenorhabditis elegans BAH-1 Is a DUF23 Protein Expressed in Seam Cells and Required for Microbial Biofilm Binding to the Cuticle

The cuticle of Caenorhabditis elegans, a complex, multi-layered extracellular matrix, is a major interface between the animal and its environment. Biofilms produced by the bacterial genus Yersinia attach to the cuticle of the worm, providing an assay for surface characteristics. A C. elegans gene required for biofilm attachment, bah-1, encodes a protein containing the domain of unknown function DUF23. The DUF23 domain is found in 61 predicted proteins in C. elegans, which can be divided into three distinct phylogenetic clades. bah-1 is expressed in seam cells, which are among the hypodermal cells that synthesize the cuticle, and is regulated by a TGF-β signaling pathway

Potent Tetrahydroquinolone Eliminates Apicomplexan Parasites

Apicomplexan infections cause substantial morbidity and mortality, worldwide. New, improved therapies are needed. Herein, we create a next generation anti-apicomplexan lead compound, JAG21, a tetrahydroquinolone, with increased sp3-character to improve parasite selectivity. Relative to other cytochrome b inhibitors, JAG21 has improved solubility and ADMET properties, without need for pro-drug. JAG21 significantly reduces Toxoplasma gondii tachyzoites and encysted bradyzoites in vitro, and in primary and established chronic murine infections. Moreover, JAG21 treatment leads to 100% survival. Further, JAG21 is efficacious against drug-resistant Plasmodium falciparum in vitro. Causal prophylaxis and radical cure are achieved after P. berghei sporozoite infection with oral administration of a single dose (2.5 mg/kg) or 3 days treatment at reduced dose (0.625 mg/kg/day), eliminating parasitemia, and leading to 100% survival. Enzymatic, binding, and co-crystallography/pharmacophore studies demonstrate selectivity for apicomplexan relative to mammalian enzymes. JAG21 has significant promise as a pre-clinical candidate for prevention, treatment, and cure of toxoplasmosis and malaria

Are there independent predisposing factors for postoperative infections following open heart surgery?

<p>Abstract</p> <p>Background</p> <p>Nosocomial infections after cardiac surgery represent serious complications associated with substantial morbidity, mortality and economic burden. This study was undertaken to evaluate the frequency, characteristics, and risk factors of microbiologically documented nosocomial infections after cardiac surgery in a Cardio-Vascular Intensive Care Unit (CVICU).</p> <p>Methods</p> <p>All patients who underwent open heart surgery between May 2006 and March 2008 were enrolled in this prospective study. Pre-, intra- and postoperative variables were collected and examined as possible risk factors for development of nosocomial infections. The diagnosis of infection was always microbiologically confirmed.</p> <p>Results</p> <p>Infection occurred in 24 of 172 patients (13.95%). Out of 172 patients, 8 patients (4.65%) had superficial wound infection at the sternotomy site, 5 patients (2.9%) had central venous catheter infection, 4 patients (2.32%) had pneumonia, 9 patients (5.23%) had bacteremia, one patient (0.58%) had mediastinitis, one (0.58%) had harvest surgical site infection, one (0.58%) had urinary tract infection, and another one patient (0.58%) had other major infection. The mortality rate was 25% among the patients with infection and 3.48% among all patients who underwent cardiac surgery compared with 5.4% of patients who did not develop early postoperative infection after cardiac surgery. Culture results demonstrated equal frequencies of gram-positive cocci and gram-negative bacteria. A backward stepwise multivariable logistic regression model analysis identified diabetes mellitus (OR 5.92, CI 1.56 to 22.42, p = 0.009), duration of mechanical ventilation (OR 1.30, CI 1.005 to 1.69, p = 0.046), development of severe complications in the CICU (OR 18.66, CI 3.36 to 103.61, p = 0.001) and re-admission to the CVICU (OR 8.59, CI 2.02 to 36.45, p = 0.004) as independent risk factors associated with development of nosocomial infection after cardiac surgery.</p> <p>Conclusions</p> <p>We concluded that diabetes mellitus, the duration of mechanical ventilation, the presence of complications irrelevant to the infection during CVICU stay and CVICU re-admission are independent risk factors for the development of postoperative infection in cardiac surgery patients.</p

Probing the Mutational Interplay between Primary and Promiscuous Protein Functions: A Computational-Experimental Approach

Protein promiscuity is of considerable interest due its role in adaptive metabolic plasticity, its fundamental connection with molecular evolution and also because of its biotechnological applications. Current views on the relation between primary and promiscuous protein activities stem largely from laboratory evolution experiments aimed at increasing promiscuous activity levels. Here, on the other hand, we attempt to assess the main features of the simultaneous modulation of the primary and promiscuous functions during the course of natural evolution. The computational/experimental approach we propose for this task involves the following steps: a function-targeted, statistical coupling analysis of evolutionary data is used to determine a set of positions likely linked to the recruitment of a promiscuous activity for a new function; a combinatorial library of mutations on this set of positions is prepared and screened for both, the primary and the promiscuous activities; a partial-least-squares reconstruction of the full combinatorial space is carried out; finally, an approximation to the Pareto set of variants with optimal primary/promiscuous activities is derived. Application of the approach to the emergence of folding catalysis in thioredoxin scaffolds reveals an unanticipated scenario: diverse patterns of primary/promiscuous activity modulation are possible, including a moderate (but likely significant in a biological context) simultaneous enhancement of both activities. We show that this scenario can be most simply explained on the basis of the conformational diversity hypothesis, although alternative interpretations cannot be ruled out. Overall, the results reported may help clarify the mechanisms of the evolution of new functions. From a different viewpoint, the partial-least-squares-reconstruction/Pareto-set-prediction approach we have introduced provides the computational basis for an efficient directed-evolution protocol aimed at the simultaneous enhancement of several protein features and should therefore open new possibilities in the engineering of multi-functional enzymes