Stability of Affine G-varieties and Irreducibility in Reductive Groups

Let $G$ be a reductive affine algebraic group, and let $X$ be an affine algebraic $G$-variety. We establish a (poly)stability criterion for points $x\in X$ in terms of intrinsically defined closed subgroups $H_{x}$ of $G$, and relate it with the numerical criterion of Mumford, and with Richardson and Bate-Martin-R\"ohrle criteria, in the case $X=G^{N}$. Our criterion builds on a close analogue of a theorem of Mundet and Schmitt on polystability and allows the generalization to the algebraic group setting of results of Johnson-Millson and Sikora about complex representation varieties of finitely presented groups. By well established results, it also provides a restatement of the non-abelian Hodge theorem in terms of stability notions.Comment: 29 pages. To appear in Int. J. Math. Note: this version 4 is identical with version 2 (version 3 is empty

Oral Health in Down Syndrome

Oral health in Down Syndrome (DS) individuals has some peculiar aspects that must be considered in the follow up of these patients. In this chapter, we will focus on the oral and maxillofacial morphological alteration, the most prevalent oral pathologies as well as preventive measures and strategies for pathologies management in this population. Also, future research on oral health of DS will be discussed

(Sub)representação política feminina e a participação das mulheres em espaços democráticos: examinando conselhos públicos

O artigo parte da observação acerca das diferenças historicamente estabelecidas entre homens e mulheres no campo social, evidenciando que o espaço público foi tomado como o locus masculino por excelência, enquanto o espaço privado e doméstico foi o ambiente destinado à mulher. Diante desse cenário, compreende-se a baixa representatividade das mulheres em espaços de poder, algo contestado pelo movimento feminista, que almeja a ampliação do papel da mulher na dimensão política, bem como a ampliação de direitos e a isonomia no tratamento da mulher na sociedade. Observou-se, ainda, no Brasil, a participação das mulheres em espaços de experiências democráticos participativos, sobretudo no âmbito de Conselhos Gestores locais. Nessa perspectiva, o objetivo do trabalho é abordar a sub-representação feminina na política, bem como examinar a qualidade da participação de mulheres nos espaços democráticos. A metodologia é lógica-dedutiva. O trabalho se inicia com a construção histórico-social do papel da mulher na sociedade e perpassa as questões relativas ao movimento feminista. Em seguida, aborda a instrumentalização da participação social no Brasil, evidenciando a representação feminina nesses espaços. Conclui-se que, ainda que tenha ocorrido relevante avanço para as mulheres no espaço público com sua crescente inserção em Conselhos locais, a arena pública ainda é majoritariamente masculina, evidenciando-se, ainda, que os espaços ocupados pelas mulheres possuem intrínseca relação com questões domésticas

Stabilization of white grape must by application of supercritical CO2 as a strategy to reduce the SO2 content

Winemaking worldwide, and especially the one carried out in warm climates, is looking for strategies to prevent enzymatic and microbial alterations of must and wines alternative to SO2 addition. However, there is any methodology as efficient as SO2, being used in combination with those substances in the best of cases, which does not suppose a real solution to the problem. In search of safer alternatives to preserve enological products, high-pressure treatments using supercritical CO2 can be a sustainable alternative The aim of this study is to analyze the biocidal effect, and enzymatic inactivation of supercritical CO2 on white grape must (Pedro Ximenez variety), in order to reduce SO2 in the pre-fermentative stage. It was processed by varying the main variable of the process, the percentage of CO2 (10, 40, and 70%), keeping the rest of the variables constant (100 bar, 35 ºC 10 min). Results are very promising and any of the treatment conditions seem to alter the physicochemical characteristics of must. Regarding must stabilization, this technique has proven to be very efficient in the reduction of total microorganisms, as well as reduce enzymatic activity of polyphenol oxidase, showing better results than those obtain when using SO2

Jardins Botânicos Portugueses: O Jardim Botânico do Faial

O livro é em português e em inglêsCâmara Municipal de Lisboainfo:eu-repo/semantics/publishedVersio

Anti-PGL1 salivary IgA/IgM, serum IgG/IgM, and nasal Mycobacterium leprae DNA in individuals with household contact with leprosy

Objectives: Leprosy household contacts represent a group at high risk of developing the disease. the aim of this study was to detect Mycobacterium leprae subclinical infection in this group through serological and molecular parameters.Methods: Serum anti-PGL1 IgG/IgM and salivary anti-PGL1 IgA/IgM was investigated using an ELISA, and nasal carriage of M. leprae DNA was detected by PCR, in leprosy household contacts of paucibacillary (PB) and multibacillary (MB) household leprosy patients (n = 135), their index cases (n = 30), and in persons living in a low endemic city (n = 17).Results: Salivary anti-PGL1 IgA and IgM and serum anti-PGL1 IgG showed good correlation comparing contacts and index cases (p 0.05). A high frequency of anti-PGL1 IgM positivity was found in IgG-negative samples (p < 0.0001). for IgG-positive samples, IgM antibodies were also positive in most of the samples. None of the 17 volunteers living in a low endemic city presented seropositivity for IgG; however, two of them showed positivity for anti-PGL1 IgM. M. leprae DNA was found in the nasal swabs of nine out of the 85 MB household leprosy contacts (10.6%) and in three out of the 50 PB household leprosy contacts (6.0%).Conclusion: We strongly suggest that serum IgG/IgM and salivary anti-PGL1 IgA/IgM measurements are used to follow leprosy household contacts. (C) 2013 International Society for Infectious Diseases. Published by Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Ceara, Fac Pharm, Dept Clin Anal & Toxicol, BR-60430370 Fortaleza, Ceara, BrazilUniv Fed Ceara, Fac Pharm, Dept Pharm, BR-60430370 Fortaleza, Ceara, BrazilUniversidade Federal de São Paulo, Div Pediat Infect Dis, São Paulo, BrazilUniv Fed Ceara, Fac Med, Dept Pathol, BR-60430370 Fortaleza, Ceara, BrazilUniversidade Federal de São Paulo, Div Pediat Infect Dis, São Paulo, BrazilCNPq: 472.471/2007-4Web of Scienc

Preventive measures of the COVID-19 adopted by the health professional in the family coexistence / Medidas de prevenção da COVID-19 adotadas pelos profissionais de saúde no convívio familiar

Objective: analyse non-pharmacological measures of prevention of the Covid-19 adopted by health professionals in the Primary Attention to Health in family coexistence. Methods: transversal and analytical study performed with 229 health professionals of the Primary Attention. Data were collected through a virtual questionnaire in the platform SurveyMonkey. The data analysis was performed by the Chi-Square Test, exact of Fisher and logistic regression. Results: most of the professionals used hygiene measures of hands, environment cleaning, food hygiene, use of tissue masks and physical distancing of relatives. Male single/divorced health professionals have smaller chance to adopt environment cleaning, food hygiene and use of tissue masks. Conclusion: the adoption of non-pharmacological measures against Covid-19 by health professionals in family coexistence is essential for reducing contamination risk among their relatives.Objetivo: analizar las medidas no farmacológicas de prevención del Covid-19 adoptadas por los profesionales de salud en la Atención Primaria de Salud en la convivencia familiar. Métodos: estudio transversal y analítico realizado con 229 profesionales de salud de la Atención Primaria. Los datos fueron recolectados con el cuestionario virtual en la plataforma SurveyMonkey. El análisis de datos se realizó mediante el Test Chi-Cuadrado, exacto de Fisher y regresión logística. Resultados: la mayoría de los profesionales utilizó medidas de higiene de manos, limpieza ambiental, higiene alimentaria, uso de mascarillas de tejido y distanciamiento físico de familiares. Los profesionales de la salud varones solteros / divorciados tienen menos posibilidades de adoptar la limpieza ambiental, la higiene alimentaria y el uso de mascarillas de tejido. Conclusión: la adopción de medidas no farmacológicas frente al Covid-19 por profesionales de salud en la convivencia familiar es fundamental para reducir la contaminación entre sus familiares.Objetivo: analisar as medidas não farmacológicas de prevenção da Covid-19 adotadas pelos profissionais de saúde da Atenção Primária à Saúde no convívio familiar. Métodos: estudo transversal, analítico, realizado com 229 profissionais de saúde da Atenção Primária. Os dados foram coletados por meio de um questionário virtual na plataforma Survey Monkey. Teste Qui-Quadrado, exato de Fisher e regressão logística foram utilizados para as análises de dados. Resultados: a maioria dos profissionais utilizou medidas como higiene das mãos, limpeza de ambiente, higiene dos alimentos, uso de máscaras de tecido e distanciamento físico dos familiares. Profissionais de saúde do sexo masculino e solteiros/divorciados tem uma menor chance para adotar a limpeza do ambiente, higiene dos alimentos e uso de máscaras de tecido. Conclusão: a adoção das medidas não farmacológicas contra a Covid-19 pelos profissionais de saúde em seu convívio familiar é fundamental para a diminuição do risco de contágio entre seus familiares.

Genetic and microenvironmental intra-tumor heterogeneity impacts colorectal cancer evolution and metastatic development

© The Author(s) 2022 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Colorectal cancer (CRC) is a highly diverse disease, where different genomic instability pathways shape genetic clonal diversity and tumor microenvironment. Although intra-tumor heterogeneity has been characterized in primary tumors, its origin and consequences in CRC outcome is not fully understood. Therefore, we assessed intra- and inter-tumor heterogeneity of a prospective cohort of 136 CRC samples. We demonstrate that CRC diversity is forged by asynchronous forms of molecular alterations, where mutational and chromosomal instability collectively boost CRC genetic and microenvironment intra-tumor heterogeneity. We were able to depict predictor signatures of cancer-related genes that can foresee heterogeneity levels across the different tumor consensus molecular subtypes (CMS) and primary tumor location. Finally, we show that high genetic and microenvironment heterogeneity are associated with lower metastatic potential, whereas late-emerging copy number variations favor metastasis development and polyclonal seeding. This study provides an exhaustive portrait of the interplay between genetic and microenvironment intra-tumor heterogeneity across CMS subtypes, depicting molecular events with predictive value of CRC progression and metastasis development.This work was financed by national funds from FCT - Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. This research was also funded by: PTDC/MED-ONC/28660/2017 from Fundação para a Ciência e Tecnologia (FCT) to A.R.G. A.R.G is recipient of Researcher Grant CEECIND/02699/2017 from FCT. The biobanking of CRC samples from Hospital Santa Maria, Lisbon, Portugal was supported by FCT research grant PIC/IC/82821/2007. This work was produced with the support of INCD funded by FCT and FEDER under the project 22153-01/SAICT/2016.info:eu-repo/semantics/publishedVersio

HERVs establish a distinct molecular subtype in stage II/III colorectal cancer with poor outcome

© The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Colorectal cancer (CRC) is one of the most lethal malignancies. The extreme heterogeneity in survival rate is driving the need for new prognostic biomarkers. Human endogenous retroviruses (hERVs) have been suggested to influence tumor progression, oncogenesis and elicit an immune response. We examined multiple next-generation sequencing (NGS)-derived biomarkers in 114 CRC patients with paired whole-exome and whole-transcriptome sequencing (WES and WTS, respectively). First, we demonstrate that the median expression of hERVs can serve as a potential biomarker for prognosis, relapse, and resistance to chemotherapy in stage II and III CRC. We show that hERV expression and CD8+ tumor-infiltrating T-lymphocytes (TILs) synergistically stratify overall and relapse-free survival (OS and RFS): the median OS of the CD8-/hERV+ subgroup was 29.8 months compared with 37.5 months for other subgroups (HR = 4.4, log-rank P < 0.001). Combing NGS-based biomarkers (hERV/CD8 status) with clinicopathological factors provided a better prediction of patient survival compared to clinicopathological factors alone. Moreover, we explored the association between genomic and transcriptomic features of tumors with high hERV expression and establish this subtype as distinct from previously described consensus molecular subtypes of CRC. Overall, our results underscore a previously unknown role for hERVs in leading to a more aggressive subtype of CRC.The biobanking of CRC from Hospital Santa Maria, Lisbon, Portugal, was supported by a grant from the Official Portuguese Funding Agency for Science and Technology (FCT: PIC/IC/82821/2007).info:eu-repo/semantics/publishedVersio