Fumigant Toxicity and Oviposition Deterrency of the Essential Oil from Cardamom, Elettaria cardamomum, Against Three Stored—product Insects

Use of insecticides can have disruptive effects on the environment. Replacing the chemical compounds in these insecticides with plant materials, however, can be a safe method with low environmental risk. In the current study, chemical composition and insecticidal activities of the essential oil from cardamom, Elettaria cardamomum L. (Maton) (Zingiberales: Zingiberaceae) on the adults of three stored product pests was investigated. Results indicated that essential oil of E. cardamomum toxic to the bruchid beetle, Callosobruchus maculatus Fabricius (Coleoptera: Bruchidae), the red flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae), and the flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Adults of E. kuehniella were more sensitive than the Coleoptera. Also, the highest mortality of these insects was seen after 12 hours. Results of the LT50 tests showed that the lethal time of mortality occurred between 10–20 hours in various test concentrations. Essential oil of E. cardamomum had a good efficacy on oviposition deterrence of C. maculatus females, too. The chemical constituents of the essential oils were analyzed by gas chromatography—mass spectrometry. The major constituents of cardamom were identified as 1,8-cineol, α-terpinyl acetate, terpinene and fenchyl alcohol. These results suggest that essential oil of E. cardamomum is a good choice for control of stored product pests

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

p53 and its major E3 ligase Mdm2 are both ubiquitinated and targeted to the proteasome for degradation. Despite the importance of this in regulating the p53 pathway, little is known about the mechanisms of proteasomal recognition of ubiquitinated p53 and Mdm2. In this study, we show that knockdown of the proteasomal ubiquitin receptor S5a/PSMD4/Rpn10 inhibits p53 protein degradation and results in the accumulation of ubiquitinated p53. Overexpression of a dominant-negative deletion of S5a lacking its ubiquitin-interacting motifs (UIM)s, but which can be incorporated into the proteasome, also causes the stabilization of p53. Furthermore, small-interferring RNA (siRNA) rescue experiments confirm that the UIMs of S5a are required for the maintenance of low p53 levels. These observations indicate that S5a participates in the recognition of ubiquitinated p53 by the proteasome. In contrast, targeting S5a has no effect on the rate of degradation of Mdm2, indicating that proteasomal recognition of Mdm2 can be mediated by an S5a-independent pathway. S5a knockdown results in an increase in the transcriptional activity of p53. The selective stabilization of p53 and not Mdm2 provides a mechanism for p53 activation. Depletion of S5a causes a p53-dependent decrease in cell proliferation, demonstrating that p53 can have a dominant role in the response to targeting S5a. This study provides evidence for alternative pathways of proteasomal recognition of p53 and Mdm2. Differences in recognition by the proteasome could provide a means to modulate the relative stability of p53 and Mdm2 in response to cellular signals. In addition, they could be exploited for p53-activating therapies. This work shows that the degradation of proteins by the proteasome can be selectively dependent on S5a in human cells, and that this selectivity can extend to an E3 ubiquitin ligase and its substrate

H2S biosynthesis and catabolism: new insights from molecular studies

Hydrogen sulfide (H2S) has profound biological effects within living organisms and is now increasingly being considered alongside other gaseous signalling molecules, such as nitric oxide (NO) and carbon monoxide (CO). Conventional use of pharmacological and molecular approaches has spawned a rapidly growing research field that has identified H2S as playing a functional role in cell-signalling and post-translational modifications. Recently, a number of laboratories have reported the use of siRNA methodologies and genetic mouse models to mimic the loss of function of genes involved in the biosynthesis and degradation of H2S within tissues. Studies utilising these systems are revealing new insights into the biology of H2S within the cardiovascular system, inflammatory disease, and in cell signalling. In light of this work, the current review will describe recent advances in H2S research made possible by the use of molecular approaches and genetic mouse models with perturbed capacities to generate or detoxify physiological levels of H2S gas within tissue

Approaches in biotechnological applications of natural polymers

Natural polymers, such as gums and mucilage, are biocompatible, cheap, easily available and non-toxic materials of native origin. These polymers are increasingly preferred over synthetic materials for industrial applications due to their intrinsic properties, as well as they are considered alternative sources of raw materials since they present characteristics of sustainability, biodegradability and biosafety. As definition, gums and mucilages are polysaccharides or complex carbohydrates consisting of one or more monosaccharides or their derivatives linked in bewildering variety of linkages and structures. Natural gums are considered polysaccharides naturally occurring in varieties of plant seeds and exudates, tree or shrub exudates, seaweed extracts, fungi, bacteria, and animal sources. Water-soluble gums, also known as hydrocolloids, are considered exudates and are pathological products; therefore, they do not form a part of cell wall. On the other hand, mucilages are part of cell and physiological products. It is important to highlight that gums represent the largest amounts of polymer materials derived from plants. Gums have enormously large and broad applications in both food and non-food industries, being commonly used as thickening, binding, emulsifying, suspending, stabilizing agents and matrices for drug release in pharmaceutical and cosmetic industries. In the food industry, their gelling properties and the ability to mold edible films and coatings are extensively studied. The use of gums depends on the intrinsic properties that they provide, often at costs below those of synthetic polymers. For upgrading the value of gums, they are being processed into various forms, including the most recent nanomaterials, for various biotechnological applications. Thus, the main natural polymers including galactomannans, cellulose, chitin, agar, carrageenan, alginate, cashew gum, pectin and starch, in addition to the current researches about them are reviewed in this article.. }To the Conselho Nacional de Desenvolvimento Cientfíico e Tecnológico (CNPq) for fellowships (LCBBC and MGCC) and the Coordenação de Aperfeiçoamento de Pessoal de Nvíel Superior (CAPES) (PBSA). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and COMPETE 2020 (POCI-01-0145-FEDER-006684) (JAT)

The ALMA REBELS Survey: Average [C ii] 158 μm Sizes of Star-forming Galaxies from z ∼ 7 to z ∼ 4

We present the average [C ii] 158 μm emission line sizes of UV-bright star-forming galaxies at z ∼ 7. Our results are derived from a stacking analysis of [C ii] 158 μm emission lines and dust continua observed by the Atacama Large Millimeter/submillimeter Array (ALMA), taking advantage of the large program Reionization Era Bright Emission Line Survey. We find that the average [C ii] emission at z ∼ 7 has an effective radius re of 2.2 ± 0.2 kpc. It is ≳2× larger than the dust continuum and the rest-frame UV emission, in agreement with recently reported measurements for z ≲ 6 galaxies. Additionally, we compared the average [C ii] size with 4 < z < 6 galaxies observed by the ALMA Large Program to INvestigate [C ii] at Early times (ALPINE). By analyzing [C ii] sizes of 4 < z < 6 galaxies in two redshift bins, we find an average [C ii] size of re = 2.2 ± 0.2 kpc and re = 2.5 ± 0.2 kpc for z ∼ 5.5 and z ∼ 4.5 galaxies, respectively. These measurements show that star-forming galaxies, on average, show no evolution in the size of the [C ii] 158 μm emitting regions at redshift between z ∼ 7 and z ∼ 4. This finding suggests that the star-forming galaxies could be morphologically dominated by gas over a wide redshift range

Effects of fluoxetine on functional outcomes after acute stroke (FOCUS): a pragmatic, double-blind, randomised, controlled trial

Background Results of small trials indicate that fluoxetine might improve functional outcomes after stroke. The FOCUS trial aimed to provide a precise estimate of these effects. Methods FOCUS was a pragmatic, multicentre, parallel group, double-blind, randomised, placebo-controlled trial done at 103 hospitals in the UK. Patients were eligible if they were aged 18 years or older, had a clinical stroke diagnosis, were enrolled and randomly assigned between 2 days and 15 days after onset, and had focal neurological deficits. Patients were randomly allocated fluoxetine 20 mg or matching placebo orally once daily for 6 months via a web-based system by use of a minimisation algorithm. The primary outcome was functional status, measured with the modified Rankin Scale (mRS), at 6 months. Patients, carers, health-care staff, and the trial team were masked to treatment allocation. Functional status was assessed at 6 months and 12 months after randomisation. Patients were analysed according to their treatment allocation. This trial is registered with the ISRCTN registry, number ISRCTN83290762. Findings Between Sept 10, 2012, and March 31, 2017, 3127 patients were recruited. 1564 patients were allocated fluoxetine and 1563 allocated placebo. mRS data at 6 months were available for 1553 (99·3%) patients in each treatment group. The distribution across mRS categories at 6 months was similar in the fluoxetine and placebo groups (common odds ratio adjusted for minimisation variables 0·951 [95% CI 0·839–1·079]; p=0·439). Patients allocated fluoxetine were less likely than those allocated placebo to develop new depression by 6 months (210 [13·43%] patients vs 269 [17·21%]; difference 3·78% [95% CI 1·26–6·30]; p=0·0033), but they had more bone fractures (45 [2·88%] vs 23 [1·47%]; difference 1·41% [95% CI 0·38–2·43]; p=0·0070). There were no significant differences in any other event at 6 or 12 months. Interpretation Fluoxetine 20 mg given daily for 6 months after acute stroke does not seem to improve functional outcomes. Although the treatment reduced the occurrence of depression, it increased the frequency of bone fractures. These results do not support the routine use of fluoxetine either for the prevention of post-stroke depression or to promote recovery of function. Funding UK Stroke Association and NIHR Health Technology Assessment Programme

Foam in iron and steelmaking

A review. Foam is common in Fe and steelmaking processes. Fe and steelmaking slags have inherent foaminess but the presence of strong surface active compounds like P2O5, Cr2O3, CaS, V2O5, and CaF2 make the foam more stable. Stable foam can form only in the presence of surface active agents. When a bubble rises on the surface of a liquid, the film around it develops a concentration gradient of surface active agents such that Marangoni force acts in upward direction. This prevents liquid drainage from the film. The presence of surface active agents favors formation of smaller bubbles and prevents bubble rupture as well. So a strong surface active agent where ds/dc is high makes the foam very stable. When a bubble comes into contact with a solid surface, the dynamic contact angle is different from the equil. value. If the former is less than the latter, there is a resultant outward force which tries to make the bubble flat. This force is responsible for the collapse of foamy slags in the presence of large carbonaceous particles. This force is not significant for very small bubbles and is not effective when carbon particles are much smaller than the bubble

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Not AvailableWorldwide, global databases on fishes lack fish diversity information at regional scale of a country, which hasfascinated many fishery workers to know the regional scale fish diversity. Databases are essential part of thebiodiversity science and have been used widely in the biological research. The present study discusses development,services and utility of the database application (UPFBase) providing information on the freshwater fish biodiversityof Uttar Pradesh. To develop UPFBase, data on the fish biodiversity of this region was compiled from differentsources that include primary data generated from different projects and secondary data from published literatures.The collected data were screened and then digitized. Microsoft ACCESS relational database and Visual Basiclanguage technologies were used for designing and implementing the standalone database application with the datamanagement capability. Presently, UPFBase provides taxonomy, synonyms, local name, common name, morphology,biology, distribution, habitat, economic importance, conservation status and other fishery information on 129 fishesbelonging to 11 orders and 27 families. UPFBase is user friendly and provides ease in working through search,query and action command button tools. It can easily be deployed on the mobile storages devices like CD-ROM,Pen drive, PCMCIA etc and can be installed on any Windows based Intel x86 machines. This version of UPFBasewas built for countries, where computational hardware and software resources are in scare and it is expected that itmight play imperious role in knowing and managing the indigenous fish diversity for decision making and posteriNot Availabl

Novel laser surface treatment approach to suppress sensitisation in modified type 316(N) stainless steel weld metal

Welded components are subjected to solution annealing heat treatment for achieving full stress relief and restoration of mechanical properties and corrosion resistance. During such heat treatments, optimum cooling rate has to be selected because very slow cooling rate will result in sensitisation and susceptibility to intergranular corrosion whereas fast cooling will result in reintroduction of residual stress. For 316 LN stainless steel which is welded using modified E316-15 electrodes (0.045-0.055%C), critical cooling rate above which there is no risk of sensitisation is 75 K h(-1). This paper presents a novel laser surface treatment which suppresses sensitisation in weld metal, even at a slower cooling rate of 65 K h(-1). Experiments involving laser surface melting were carried out with 150 W average power pulsed Nd:YAG laser and 10 kW CO2 laser, in both continuous wave and pulse modulated (100 Hz) modes. Best results were obtained when surface melting was performed with high frequency pulse modulated CO2 laser beam. The processed weld metal remained unsensitised after solution annealing followed by slower rate of cooling at 65 K h(-1). Numerical simulation study was performed with ANSYS 7.0 software to understand the physical reason behind the difference in sensitisation behaviour of CO2 laser melted specimens under continuous wave and high frequency pulse modulated conditions and the predictions were validated using results of electron backscattered diffraction studies. Weld metal specimens treated with high frequency pulse modulated CO2 laser clearly showed evolution of fine grains near the fusion boundary region which enhanced sensitisation resistance