Nocturnal Behavior of Clark’s Spiny Lizard (Sceloporus clarkii; Squamata; Lacertilia) in Acaponeta, Nayarit, México

There are several documented reports of nocturnal activity in reptiles considered exclusively diurnal (Lara- Resendiz 2020, Perry et al. 2008). Clark?s Spiny Lizard (Sceloporus clarkii) is considered a diurnal active species, but there is a single observation of an adult male attempting to feed on moths at night over a two-hour period (Martínez-Méndez et al. 2013). Herein we report on additional nocturnal activities of S. clarkii and include descriptive thermal data.Fil: Loc Barragán, Jesús A.. Tecnológico Nacional de México. Instituto Tecnológico Superior de Zacapoaxtla. Maestría en Ciencias Ambientales; MéxicoFil: Lara Resendiz, Rafael Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Woolrich Piña, Guillermo A.. Tecnológico Nacional de México. Instituto Tecnológico Superior de Zacapoaxtla; Méxic

Extracellular vesicles - mediating and delivering cardioprotection in acute myocardial infarction and heart failure

New treatments are urgently needed to reduce myocardial infarct size and prevent adverse post-infarct left ventricular remodeling, in order to preserve cardiac function, and prevent the onset of heart failure in patients presenting with acute myocardial infarction (AMI). In this regard, extracellular vesicles (EVs) have emerged as key mediators of cardioprotection. Endogenously produced EVs are known to play crucial roles in maintaining normal cardiac homeostasis and function, by acting as mediators of intercellular communication between different types of cardiac cells. Endogenous EVs have also been shown to contribute to innate cardioprotective strategies such as remote ischemic conditioning. In terms of EV-based therapeutics, stem cell-derived EVs have been shown to confer cardioprotection in a large number of small and large animal AMI models, and have the therapeutic potential to be applied in the clinical setting for the benefit of AMI patients, although several challenges need to be overcome. Finally, EVs may be used as vehicles to deliver therapeutics to the infarcted heart, providing a potential synergist approach to cardioprotection. In this review article, we highlight the various roles that EVs play as mediators and deliverers of cardioprotection, and discuss their therapeutic potential for improving clinical outcomes following AMI

Porcine Reproductive and Respiratory Syndrome Virus Infects Mature Porcine Dendritic Cells and Up-Regulates Interleukin-10 Production

Porcine reproductive and respiratory syndrome virus (PRRSV) infects mature dendritic cells (mDCs) derived from porcine monocytes and matured with lipopolysaccharide. The infection of mDCs induced apoptosis, reduced the expression of CD80/86 and major histocompatibility complex class II molecules, and increased the expression of interleukin-10, thus suggesting that such mDC modulation results in the impairment of T-cell activation

Water-soluble polyphosphonate-based bottlebrush copolymers via aqueous ring-opening metathesis polymerization

Ring-opening metathesis polymerization (ROMP) is a versatile method for synthesizing complex macromolecules from various functional monomers. In this work, we report the synthesis of water-soluble and degradable bottlebrush polymers, based on polyphosphoesters (PPEs) via ROMP. First, PPE-macromonomers were synthesized via organocatalytic anionic ring-opening polymerization of 2-ethyl-2-oxo-1,3,2-dioxaphospholane using N-(hydroxyethyl)-cis-5-norbornene-exo-2,3-dicarboximide as the initiator and 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) as the catalyst. The resulting norbornene-based macromonomers had degrees of polymerization (DPn) ranging from 25 to 243 and narrow molar mass dispersity (Đ ≤ 1.10). Subsequently, these macromonomers were used in ROMP with the Grubbs 3rd-generation bispyridyl complex (Ru-G3) to produce a library of well-defined bottlebrush polymers. The ROMP was carried out either in dioxane or in aqueous conditions, resulting in well-defined and water-soluble bottlebrush PPEs. Furthermore, a two-step protocol was employed to synthesize double hydrophilic diblock bottlebrush copolymers via ROMP in water at neutral pH-values. This general protocol enabled the direct combination of PPEs with ROMP to synthesize well-defined bottlebrush polymers and block copolymers in water. Degradation of the PPE side chains was proven resulting in low molar mass degradation products only. The biocompatible and biodegradable nature of PPEs makes this pathway promising for designing novel biomedical drug carriers or viscosity modifiers, as well as many other potential applications.</p

Effect of the microstructure generated by repetitive corrugation and straightening (RCS) process on the mechanical properties and stress corrosion cracking of Al-7075 alloy

This study discussed the effect of the heterogeneous microstructure generated through the Repetitive Corrugation and Straightening (RCS) process on the mechanical and stress corrosion cracking behavior of the AA7075. As a result of the RCS process, significant grain refinement was obtained. The average grain size ranged from 126 to 59 µm, for the initial condition and 4 RCS passes, respectively. The yield strength and hardness increased 170% and 15% from the initial pass, remaining almost constant afterward. The evaluation of stress corrosion cracking showed a decrement in the number of cracks of 21.6% and 23.5% between the initial condition and fourth RCS passes. The cracking and pitting corrosion were the dominant mechanisms in the tested samples. The mechanical and corrosion results were also discussed in terms of the microstructural features.Peer ReviewedPostprint (published version

Rainfall-induced differential settlements of foundations on heterogeneous unsaturated soils

This study stochastically investigates the rainfall-induced differential settlement of a centrally loaded, rigid strip foundation on an unsaturated soil with spatially varying values of either preconsolidation stress or porosity. The differential settlement (between the two foundation ends) is calculated at various times during rainfall by way of a coupled, hydro-mechanical, finite-element analysis. The Barcelona basic model describes the mechanical behaviour of the soil, and the van Genuchten relationships describe water retention and permeability. The variability of soil properties is modelled by means of random fields with spatial correlation in the framework of a Monte Carlo simulation. The study demonstrates that the occurrence of rainfall-induced differential settlements can be consistently analysed using concepts of unsaturated soil mechanics and random field theory. Results show that differential settlements can be vastly underpredicted (or even completely missed) if random heterogeneity and partial saturation are not simultaneously considered. The variation of differential settlements and their statistics during the rainfall depend on the magnitude of the applied load and the statistics of soil variability. Moreover, the transient phase of infiltration and a spatial correlation length equal to the width of the foundation pose the highest risk of differential settlement

Mechanical, stress corrosion cracking and crystallographic study on flat components processed by two combined severe plastic deformation techniques

Although the current field of application of Al–alloy 7075 (AA7075) is vast, it is still limited due to some drawbacks, especially due to its susceptibility to stress corrosion cracking (SCC). This work aims to evaluate the microstructural, mechanical, and stress corrosion cracking (SCC) behaviors on an AA7075 in flat format deformed by a combination of repetitive corrugation and straightening (RCS) and accumulative roll bonding (ARB) techniques. Four different deformation routes were applied, namely: ARB (A), RCS (R), RCS + ARB (RA) and ARB + RCS (AR). As expected, the efficiency for grain refinement depends on the applied route, in terms of average grain size regarding the initial condition IC): AR > A > RA > R. All conditions resulted in unimodal and widened grain size distributions of micro-, submicro- and nano-metric dimensions. The study of crystallographic orientations showed that route R did not generate any new texture, whereas different preferred orientations were obtained for routes A, RA, and AR. The hardness and three-point bending tests showed an improvement of mechanical strength in the following order: AR > RA > A > R. The cracks per cm2 obtained in the corrosion study indicated that the best SCC resistance was R > A > AR > RA. Based on the above, the best combination of microstructural, mechanical, and SCC properties until one deformation pass was obtained by the single route of the ARB process.Peer ReviewedPostprint (published version

Multinational tagging efforts illustrate regional scale of distribution and threats for east pacific green turtles (Chelonia mydas agassizii).

Published onlineJournal ArticleResearch Support, Non-U.S. Gov'tThis is the final version of the article. Available from Public Library of Science via the DOI in this record.To further describe movement patterns and distribution of East Pacific green turtles (Chelonia mydas agassizii) and to determine threat levels for this species within the Eastern Pacific. In order to do this we combined published data from existing flipper tagging and early satellite tracking studies with data from an additional 12 satellite tracked green turtles (1996-2006). Three of these were tracked from their foraging grounds in the Gulf of California along the east coast of the Baja California peninsula to their breeding grounds in Michoacán (1337-2928 km). In addition, three post-nesting females were satellite tracked from Colola beach, Michoacán to their foraging grounds in southern Mexico and Central America (941.3-3020 km). A further six turtles were tracked in the Gulf of California within their foraging grounds giving insights into the scale of ranging behaviour. Turtles undertaking long-distance migrations showed a tendency to follow the coastline. Turtles tracked within foraging grounds showed that foraging individuals typically ranged up to 691.6 km (maximum) from release site location. Additionally, we carried out threat analysis (using the cumulative global human impact in the Eastern Pacific) clustering pre-existing satellite tracking studies from Galapagos, Costa Rica, and data obtained from this study; this indicated that turtles foraging and nesting in Central American waters are subject to the highest anthropogenic impact. Considering that turtles from all three rookeries were found to migrate towards Central America, it is highly important to implement conservation plans in Central American coastal areas to ensure the survival of the remaining green turtles in the Eastern Pacific. Finally, by combining satellite tracking data from this and previous studies, and data of tag returns we created the best available distributional patterns for this particular sea turtle species, which emphasized that conservation measures in key areas may have positive consequences on a regional scale.The work was supported by Earthwatch Institute, David and Lucile Packard Foundation, Wallace Research Foundation, PADI Foundation and the Arizona-Sonora Desert Museum. C. E. H. received a Masters degree bursary from the University of Exeter and the European Social Fund and would like to thank Consejo Nacional de Ciencia y Tecnología (Mexico) for support through a PhD scholarship. W. J. N. was supported by a Fulbright Fellowship and a Marshall Fellowship during the period field research in Baja California was conducted. B. J. G. is supported by the Darwin Initiative, European Social Fund and The Natural Environment Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Florecimientos de microalgas planctónicas de 2000 al 2015 en la Bahía de Acapulco, Guerrero, México

Background and Aims: Harmful algal blooms (HABs) affect the marine ecosystem in multiple ways. The objective was to document the species that produced blooms in Acapulco Bay over a 15-year period (2000-2015) and analyze the presence of these events with El Niño-Southern Oscillation (ENSO).Methods: Thirty-five collections, made during the years 2000, 2002-2004, 2006-2011, 2013-2015, were undertaken with phytoplankton nets and Van Dorn bottle, yielding 526 samples, of which 423 were quantified using the Utermöhl method. The relationship of HAB with ENSO was made with standardized values of Multivariate ENSO Index (MEI) and the significance was evaluated with the method quadrant sums of Olmstead-Tukey.Key results: Using data of cell density and high relative abundance (&gt;60%), 53 blooms were recorded, most of them occurring during the rainy season (June-October) and dry-cold season (November-March), plus 37 blooms reported by other authors. These 90 blooms were composed of 40 taxa: 21 diatoms and 19 dinoflagellates, the former mostly innocuous. Sixty-seven blooms had species reported as noxious, of which 11 species commonly produce toxic HAB. Toxic taxa are Pseudo-nitzschia spp. (four taxa), and seven dinoflagellates.Conclusions: Abundance analyses of Pyrodinium bahamense var. compressum and Gymnodinium catenatum against values of MEI showed a clear tendency to produce HAB in La Niña conditions. Both taxa, producers of saxitoxins, cause paralytic shellfish poisoning (PSP) and coexist in Acapulco; therefore, they present a risk to human health. Another noxious 52 taxa found in Acapulco were currently considered potential HABs, because they have been recorded at low densities. Given the sharp differences in density values of bloom-forming species found in this work compared to those reported by other authors on similar dates, it is important to perform calibration tests to rule out possible errors in cell counts.Antecedentes y Objetivos: Los florecimientos algales nocivos (FAN) afectan al ecosistema marino de varias maneras. El objetivo fue reconocer las especies productoras de dichos florecimientos en la Bahía de Acapulco, durante un periodo de 15 años (2000-2015) y relacionar su presencia con el fenómeno El Niño-Oscilación del Sur (ENSO).Métodos: Analizamos 526 muestras de 35 colectas efectuadas durante los años 2000, 2002-2004, 2006-2011 y 2013-2015, realizadas con red y botella Van Dorn; éstas últimas (423) cuantificadas con el método de Utermöhl. La relación de FAN con ENSO se hizo con valores estandarizados del Índice Multivariado ENSO (MEI), y las significancia evaluada con el método de Asociación de Cuadrantes de Olmstead-Tukey.Resultados clave: Con base en la densidad celular y abundancia relativa alta (&gt;60%) se reconocieron 53 florecimientos, la mayoría durante la época de lluvias (junio-octubre) y secas-fría (noviembre-marzo), más otros 37 eventos reportados por distintos autores. Estos 90 eventos estuvieron conformados por 40 taxa (21 diatomeas y 19 dinoflagelados); los de diatomeas, en su mayoría, inocuos. Sesenta y siete florecimientos estuvieron conformados por especies reportadas como dañinas, de las cuales 11 comúnmente producen FAN tóxicos. Los taxa tóxicos fueron Pseudo-nitzschia spp. (cuatro taxa) y siete dinoflagelados.Conclusiones: Los análisis de abundancia de Pyrodinium bahamense var. compressum y Gymnodinium catenatum, en relación con los valores MEI, mostraron una clara tendencia a producir FAN en condiciones La Niña. Ambos taxa, productores de saxitoxinas y causantes de envenenamiento paralítico por moluscos, incluso llegan a coexistir en Acapulco, representando un riesgo para la salud humana. Otros 52 taxa dañinos por encontrarse en bajas densidades, se consideraron por el momento potenciales formadores de FAN. Dadas las agudas diferencias encontradas en valores de densidad de especies formadoras de florecimientos respecto a las reportadas por otros autores en fechas similares, es importante realizar pruebas de calibración para descartar posibles errores en conteos celulares