350 research outputs found
Evolution of a localized thermal explosion in a reactive gas
Experimental observations of ignition in premixed gaseous reactants indicate that perfectly homogeneous initiation is practically unrealizable. Instead, combustion first sets in, as a rule, at small, discrete sites where inherent inhomogeneities cause chemical activity to proceed preferentially and lead to localized explosions. Combustion waves propagating away from these hot spots or reaction centers eventually envelop the remaining bulk. This study examines the spatial structure and temporal evolution of a hot spot for a model involving Arrhenius kinetics. The hot spot, characterized by peaks in pressure and temperature with little diminution in local density, is shown to have one of two possible self-similar structures. The analysis employs a combination of asymptotics and numerics, and terminates when pressure and temperature in the explosion have peaked
Ignition of thermally sensitive explosives between a contact surface and a shock
The dynamics of ignition between a contact surface and a shock wave is investigated using a
one-step reaction model with Arrhenius kinetics. Both large activation energy asymptotics and
high-resolution finite activation energy numerical simulations are employed. Emphasis is on comparing
and contrasting the solutions with those of the ignition process between a piston and a shock,
considered previously. The large activation energy asymptotic solutions are found to be qualitatively
different from the piston driven shock case, in that thermal runaway first occurs ahead of
the contact surface, and both forward and backward moving reaction waves emerge. These waves
take the form of quasi-steady weak detonations that may later transition into strong detonation
waves. For the finite activation energies considered in the numerical simulations, the results are
qualitatively different to the asymptotic predictions in that no backward weak detonation wave
forms, and there is only a weak dependence of the evolutionary events on the acoustic impedance
of the contact surface. The above conclusions are relevant to gas phase equation of state models.
However, when a large polytropic index more representative of condensed phase explosives is used,
the large activation energy asymptotic and finite activation energy numerical results are found to
be in quantitative agreement
Reactive-diffuse System with Arrhenius Kinetics: Peculiarities of the Spherical Goemetry
The steady reactive-diffusive problem for a non isothermal permeable pellet with first-order Arrhenius kinetics is studied. In the large activation-energy limit, asymptotic solutions are derived for the spherical geometry. The solutions exhibit multiplicity and it is shown that a suitable choice of parameters can lead to an arbitrarily large number of solutions, thereby confirming a conjecture based upon past computational experiments. Explicit analytical expressions are given for the multiplicity bounds (ignition and extinction limits). The asymptotic results compare very well with those obtained numerically, even for moderate values of the activation energy
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A High-Resolution Godunov Method for Compressible Multi-Material Flow on Overlapping Grids
A numerical method is described for inviscid, compressible, multi-material flow in two space dimensions. The flow is governed by the multi-material Euler equations with a general mixture equation of state. Composite overlapping grids are used to handle complex flow geometry and block-structured adaptive mesh refinement (AMR) is used to locally increase grid resolution near shocks and material interfaces. The discretization of the governing equations is based on a high-resolution Godunov method, but includes an energy correction designed to suppress numerical errors that develop near a material interface for standard, conservative shock-capturing schemes. The energy correction is constructed based on a uniform pressure-velocity flow and is significant only near the captured interface. A variety of two-material flows are presented to verify the accuracy of the numerical approach and to illustrate its use. These flows assume an equation of state for the mixture based on Jones-Wilkins-Lee (JWL) forms for the components. This equation of state includes a mixture of ideal gases as a special case. Flow problems considered include unsteady one-dimensional shock-interface collision, steady interaction of an planar interface and an oblique shock, planar shock interaction with a collection of gas-filled cylindrical inhomogeneities, and the impulsive motion of the two-component mixture in a rigid cylindrical vessel
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A Study of Detonation Diffraction in the Ignition-and-Growth Model
Heterogeneous high-energy explosives are morphologically, mechanically and chemically complex. As such, their ab-initio modeling, in which well-characterized phenomena at the scale of the microstructure lead to a rationally homogenized description at the scale of observation, is a subject of active research but not yet a reality. An alternative approach is to construct phenomenological models, in which forms of constitutive behavior are postulated with an eye on the perceived picture of the micro-scale phenomena, and which are strongly linked to experimental calibration. Most prominent among these is the ignition-and-growth model conceived by Lee and Tarver. The model treats the explosive as a homogeneous mixture of two distinct constituents, the unreacted explosive and the products of reaction. To each constituent is assigned an equation of state, and a single reaction-rate law is prescribed for the conversion of the explosive to products. It is assumed that the two constituents are always in pressure and temperature equilibrium. The purpose of this paper is to investigate in detail the behavior of the model in situations where a detonation turns a corner and undergoes diffraction. A set of parameters appropriate for the explosive LX-17 is selected. The model is first examined analytically for steady, planar, 1-D solutions and the reaction-zone structure of Chapman-Jouguet detonations is determined. A computational study of two classes of problems is then undertaken. The first class corresponds to planar, 1-D initiation by an impact, and the second to corner turning and diffraction in planar and axisymmetric geometries. The 1-D initiation, although interesting in its own right, is utilized here as a means for interpretation of the 2-D results. It is found that there are two generic ways in which 1-D detonations are initiated in the model, and that these scenarios play a part in the post-diffraction evolution as well. For the parameter set under study the model shows detonation failure, but only locally and temporarily, and does not generate sustained dead zones. The computations employ adaptive mesh refinement and are finely resolved. Results are obtained for a rigid confinement of the explosive. Compliant confinement represents its own computational challenges and is currently under study. Also under development is an extended ignition-and-growth model which takes into account observed desensitization of heterogeneous explosives by weak shocks
Existence of radial stationary solutions for a system in combustion theory
In this paper, we construct radially symmetric solutions of a nonlinear
noncooperative elliptic system derived from a model for flame balls with
radiation losses. This model is based on a one step kinetic reaction and our
system is obtained by approximating the standard Arrehnius law by an ignition
nonlinearity, and by simplifying the term that models radiation. We prove the
existence of 2 solutions using degree theory
Scaling, Propagation, and Kinetic Roughening of Flame Fronts in Random Media
We introduce a model of two coupled reaction-diffusion equations to describe
the dynamics and propagation of flame fronts in random media. The model
incorporates heat diffusion, its dissipation, and its production through
coupling to the background reactant density. We first show analytically and
numerically that there is a finite critical value of the background density,
below which the front associated with the temperature field stops propagating.
The critical exponents associated with this transition are shown to be
consistent with mean field theory of percolation. Second, we study the kinetic
roughening associated with a moving planar flame front above the critical
density. By numerically calculating the time dependent width and equal time
height correlation function of the front, we demonstrate that the roughening
process belongs to the universality class of the Kardar-Parisi-Zhang interface
equation. Finally, we show how this interface equation can be analytically
derived from our model in the limit of almost uniform background density.Comment: Standard LaTeX, no figures, 29 pages; (to appear in J. Stat. Phys.
vol.81, 1995). Complete file available at
http://www.physics.helsinki.fi/tft/tft.html or anonymous ftp at
ftp://rock.helsinki.fi/pub/preprints/tft
Strategic Sensemaking and Political Connections in Unstable Institutional Contexts
Emerging economies are often characterized by pervasive institutional changes and resultant institutional voids. In the absence of strong formal institutions, firms rely on informal institutions to fill these voids. This article argues that the process of sensemaking for firms in turbulent environments is continuous and dependent on cyclical adjustments connecting performance via a feedback loop to scanning and interpretation. Far from being a one-time occurrence, environmental sensemaking is a process operating in accord with continuous environmental changes. This study’s findings derive from an in-depth analysis of a Russian pharmaceutical firm and an Indian telecommunications firm, and demonstrate that entrepreneurs make sense and gain legitimacy through political connections. The study further finds that improvements in institutional environments reduce the salience of political networks, thereby creating a choice for firms to rely on formed market mechanisms or continue along the path of political connections that evolve to public–private partnerships
Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
GoldenBraid (GB) is an iterative and standardized DNA assembling system specially designed for Multigene Engineering in Plant Synthetic Biology. GB is based on restriction–ligation reactions using type IIS restriction enzymes. GB comprises a collection of standard DNA pieces named “GB parts” and a set of destination plasmids (pDGBs) that incorporate the multipartite assembly of standardized DNA parts. GB reactions are extremely efficient: two transcriptional units (TUs) can be assembled from several basic GBparts in one T-DNA less than 24 h. Moreover, larger assemblies comprising 4–5 TUs are routinely built in less than 2 working weeks. Here we provide a detailed view of the GB methodology. As a practical example, a Bimolecular Fluorescence Complementation construct comprising four TUs in a 12 kb DNA fragment is presented.Sarrion-Perdigones, A.; PalacĂ, J.; Granell Richart, A.; Orzáez Calatayud, DV. (2014). Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy. Methods in Molecular Biology. 1116:133-151. doi:10.1007/978-1-62703-764-8_10S1331511116Haseloff J, Ajioka J (2009) Synthetic biology, history, challenges and prospects. J R Soc Interface 6(Suppl 4):S389–S391Check E (2005) Synthetic biology, designs on life. Nature 438:417–418Kosuri S, Eroshenko N, LeProust EM et al (2010) Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nat Biotechnol 28:1295–1299Ellis T, Adie T, Baldwin GS (2011) DNA assembly for synthetic biology, from parts to pathways and beyond. Integr Biol 3:109–118Gibson DG, Young L, Chuang R-Y et al (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6: 343–345Gibson DG, Glass JI, Lartigue C et al (2010) Creation of a bacterial cell controlled by a chemically synthesized genome. Science 329:52–56Sarrion-Perdigones A, Falconi EE, Zandalinas SI et al (2011) GoldenBraid, an iterative cloning system for standardized assembly of reusable genetic modules. PLoS One 6:e21622Sarrion-Perdigones A, Vilar-Vazquez M et al (2013) GoldenBraid2.0, A comprehensive DNA assembly framework for plant synthetic biology. Plant Physiol 162:1618–1631Engler C, Gruetzner R, Kandzia R (2009) Golden gate shuffling, a one-pot DNA shuffling method based on type IIs restriction enzymes. PLoS One 4:e5553Engler C, Kandzia R, Marillonnet S (2008) A one pot, one step, precision cloning method with high throughput capability. PLoS One 3:e3647Bracha-Drori K, Shichrur K, Katz A et al (2004) Detection of protein-protein interactions in plants using bimolecular fluorescence complementation. Plant J 40:419–427Smaczniak C, Immink RG, Muino JM et al (2012) Characterization of MADS-domain transcription factor complexes in Arabidopsis flower development. Proc Natl Acad Sci U S A 109:1560–1565de Folter S, Immink RG, Kieffer M et al (2005) Comprehensive interaction map of the Arabidopsis MADS Box transcription factors. Plant Cell 17:1424–1433Lorenz WW, McCann RO, Longiaru M et al (1991) Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc Natl Acad Sci U S A 88:4438–4442Voinnet O, Pinto YM, Baulcombe DC (1999) Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proc Natl Acad Sci U S A 96: 14147–14152Hellens RP, Edwards EA, Leyland NR et al (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42:819–832Butelli E, Titta L, Giorgio M et al (2008) Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nat Biotechnol 26: 1301–1308Kapila J, DeRycke R, VanMontagu M et al (1997) An Agrobacterium-mediated transient gene expression system for intact leaves. Plant Sci 122:101–10
The CS1 segment of fibronectin is involved in human OSCC pathogenesis by mediating OSCC cell spreading, migration, and invasion
<p>Abstract</p> <p>Background</p> <p>The alternatively spliced V region or type III connecting segment III (IIICS) of fibronectin is important in early development, wound healing, and tumorigenesis, however, its role in oral cancer has not been fully investigated. Thus, we investigated the role of CS-1, a key site within the CSIII region of fibronectin, in human oral squamous cell carcinoma (OSCC).</p> <p>Methods</p> <p>To determine the expression of CS-1 in human normal and oral SCC tissue specimens immunohistochemical analyses were performed. The expression of CS1 was then associated with clinicopathological factors. To investigate the role of CS-1 in regulating OSCC cell spreading, migration and invasion, OSCC cells were assayed for spreading and migration in the presence of a CS-1 peptide or a CS-1 blocking peptide, and for invasion using Matrigel supplemented with these peptides. In addition, integrin α4siRNA or a focal adhesion kinase (FAK) anti-sense oligonucleotide was transfected into OSCC cells to examine the mechanistic role of integrin α4 or FAK in CS1-mediated cell spreading and migration, respectively.</p> <p>Results</p> <p>CS-1 expression levels were significantly higher in OSCC tissues compared to normal tissues (p < 0.05). Also, although, high levels of CS-1 expression were present in all OSCC tissue samples, low-grade tumors stained more intensely than high grade tumors. OSCC cell lines also expressed higher levels of CS-1 protein compared to normal human primary oral keratinocytes. There was no significant difference in total fibronectin expression between normal and OSCC tissues and cells. Inclusion of CS-1 in the in vitro assays enhanced OSCC cell spreading, migration and invasion, whereas the CS1 blocking peptide inhibited these processes. Suppression of integrin α4 significantly inhibited the CS1-mediated cell spreading. Furthermore, this migration was mediated by focal adhesion kinase (FAK), since FAK suppression significantly blocked the CS1-induced cell migration.</p> <p>Conclusion</p> <p>These data indicate that the CS-1 site of fibronectin is involved in oral cancer pathogenesis and in regulating OSCC cell spreading, migration and invasion.</p
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