Impact differences among the landing phases of a drop vertical jump in soccer players

The aim of this study was to examine the differences of landing phase biomechanics between the players who had anterior cruciate ligament (ACL) reconstruction and healthy participants during single leg drop vertical jump. In this study, 11 soccer players who had anterior cruciate ligament reconstruction (aged 23.0±3.6 years, height 177±5.0 cm, weight 83.8±11.7 kg) and 9 healthy soccer players( aged 22.2±2.4 years, height 178±3.0 cm, weight 74.3±6.1 kg) participated voluntarily. During the data collection phase three high speed cameras synchronized to each other and force plate were used. Visual analysis programme and MATLAB were used to calculate kinetic and kinematic variables. Landing techniques of the subjects' were examined by flexion angle of knee, ground reaction force and moment parameters. The statistical analyses of the measured results were performed by t-test and Pearson Correlation analysis. According to the results, it was determined that peak vertical ground reaction force exhibited significant phase differences (p=0.00, and p=0.00, respectively) between the groups. Obtained results can be explained with "quadriceps avoidance" motion pattern which is characterized by decreased quadriceps activity and lower external knee flexion moment in an effort to control anterior translation of the tibia in subjects with ACL reconstruction. A better understanding of the different phases during single-leg landings can shed a light on mechanism of non-contact anterior crucaite ligament injuries therefore future researches should assess how phase differences affect drop vertical jump performance. © 2018 Montenegrin Sports Academy. All rights reserved

Reflection of electromagnetic waves from mixtures of plane gravitational and scalar waves

We consider colliding wave packets consisting of hybrid mixtures of electromagnetic, gravitational and scalar waves. Irrespective of the scalar field, the electromagnetic wave still reflects from the gravitational wave. Some reflection processes are given for different choice of packets in which the Coulomb-like component $\Psi_2$ vanishes. Exact solution for multiple reflection of an electromagnetic wave from successive impulsive gravitational waves is obtained in a closed form. It is shown that a succesive sign flip in the Maxwell spinor arises as a result of encountering with an impulsive train (i.e. the Dirac's comb curvature) of gravitational waves. Such an observable effect may be helpful in the detection of gravitational wave bursts.Comment: 20 pages, 3 ps figures, small typos corrected, published versio

Quantum singularities in a model of f(R) Gravity

The formation of a naked singularity in a model of f(R) gravity having as source a linear electromagnetic field is considered in view of quantum mechanics. Quantum test fields obeying the Klein-Gordon, Dirac and Maxwell equations are used to probe the classical timelike naked singularity developed at r=0. We prove that the spatial derivative operator of the fields fails to be essentially self-adjoint. As a result, the classical timelike naked singularity remains quantum mechanically singular when it is probed with quantum fields having different spin structures.Comment: 12 pages, final version. Accepted for publication in EPJ

Impacts of red pepper supplemented diets and different storage conditions on eggs obtained from free-range laying hens

Egg quality depends on many conditions, including diet and storage temperature. Consumers usually assess it by checking yolk colour. The aim of the study was to indicate the effects of storage periods (7, 14 and 21 days) and temperature (room and refrigerator) on egg quality parameters, especially yolk colour. The experiment was carried out with 150 eggs, which were collected from free-range Lohmann brown laying hens (42 weeks old) fed with or without 0.75% red pepper supplemented diets. The highest weight loss was observed in the eggs stored at room temperature (23 °C, 64% humidity) for 21 days. In addition, it was discovered that the highest air cell height (ACH) was detected in the eggs stored at room temperature for 14 and 21 days. Red pepper supplementation and storage time and temperature were found to have been effective on the Roche yolk colour fan values (RYCF), lightness (L*) and yellowness (b*). Additionally, red pepper supplementation had a significantly positive effect on the redness (a*) value of the yolk. However, no statistical difference in the a* value was determined between the fresh eggs and the stored eggs supplemented with red pepper. The supplementation of 0.75% red pepper as a natural colouring agent to the diets of free-range laying hens had a positive effect on the yolk colour, which is an important attribution for consumer perception. Furthermore, the colours of the yolks of eggs stored in the refrigerator for a week were not significantly different from the fresh ones. Consequently, the present study suggests feeding laying hens diets supplemented with 0.75% red pepper might be useful in improving yolk colour. The results indicate that the eggs stored in the refrigerator might be closer to the characteristics of fresh eggs. In contrast, eggs stored at room temperature showed more deterioration between treatments.Keywords: Colour evaluation methods, egg quality, free-range, yolk colou

Boron Stress Responsive MicroRNAs and Their Targets in Barley

Boron stress is an environmental factor affecting plant development and production. Recently, microRNAs (miRNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. In this study, miRNAs associated with boron stress were identified and characterized in barley. miRNA profiles were also comparatively analyzed between root and leave samples. A total of 31 known and 3 new miRNAs were identified in barley; 25 of them were found to respond to boron treatment. Several miRNAs were expressed in a tissue specific manner; for example, miR156d, miR171a, miR397, and miR444a were only detected in leaves. Additionally, a total of 934 barley transcripts were found to be specifically targeted and degraded by miRNAs. In silico analysis of miRNA target genes demonstrated that many miRNA targets are conserved transcription factors such as Squamosa promoter-binding protein, Auxin response factor (ARF), and the MYB transcription factor family. A majority of these targets were responsible for plant growth and response to environmental changes. We also propose that some of the miRNAs in barley such as miRNA408 might play critical roles against boron exposure. In conclusion, barley may use several pathways and cellular processes targeted by miRNAs to cope with boron stress

Unique and conserved MicroRNAs in wheat chromosome 5D revealed by next-generation sequencing

MicroRNAs are a class of short, non-coding, single-stranded RNAs that act as post-transcriptional regulators in gene expression. miRNA analysis of Triticum aestivum chromosome 5D was performed on 454 GS FLX Titanium sequences of flow sorted chromosome 5D with a total of 3,208,630 good quality reads representing 1.34x and 1.61x coverage of the short (5DS) and long (5DL) arms of the chromosome respectively. In silico and structural analyses revealed a total of 55 miRNAs; 48 and 42 miRNAs were found to be present on 5DL and 5DS respectively, of which 35 were common to both chromosome arms, while 13 miRNAs were specific to 5DL and 7 miRNAs were specific to 5DS. In total, 14 of the predicted miRNAs were identified in wheat for the first time. Representation (the copy number of each miRNA) was also found to be higher in 5DL (1,949) compared to 5DS (1,191). Targets were predicted for each miRNA, while expression analysis gave evidence of expression for 6 out of 55 miRNAs. Occurrences of the same miRNAs were also found in Brachypodium distachyon and Oryza sativa genome sequences to identify syntenic miRNA coding sequences. Based on this analysis, two other miRNAs: miR1133 and miR167 were detected in B. distachyon syntenic region of wheat 5DS. Five of the predicted miRNA coding regions (miR6220, miR5070, miR169, miR5085, miR2118) were experimentally verified to be located to the 5D chromosome and three of them : miR2118, miR169 and miR5085, were shown to be 5D specific. Furthermore miR2118 was shown to be expressed in Chinese Spring adult leaves. miRNA genes identified in this study will expand our understanding of gene regulation in bread wheat

Digital sculpting for historical representation: Neville tomb case study

Despite digital 3-D polygon modelling applications providing a common and powerful tool-set for archaeological, architectural and historical visualisation over recent years, the relatively recent developments in high-resolution sculpting software allow for the possibility to create digital outcomes with a degree of surface fidelity not previously obtainable from the more widely used poly-modelling software packages. Such digital sculpting applications are more commonly applied within the video games and TV/motion picture industries, the intention of this paper is to show how such tools and methodologies together with existing scanned data and some historical knowledge can remediate and re-imagine lost sculptural form. The intended research will focus on an examination and partial re-construction of the tomb of Sir John Neville, 3rd Baron Raby located at Durham Cathedral, County Durham UK

First draft genome assembly of the Argane tree (Argania spinosa)

Background: The Argane tree (Argania spinosa L. Skeels) is an endemic tree of southwestern Morocco that plays an important socioeconomic and ecologic role for a dense human population in an arid zone. Several studies confirmed the importance of this species as a food and feed source and as a resource for both pharmaceutical and cosmetic compounds. Unfortunately, the argane tree ecosystem is facing significant threats from environmental changes (global warming, over-population) and over-exploitation. Limited research has been conducted, however, on argane tree genetics and genomics, which hinders its conservation and genetic improvement. Methods: Here, we present a draft genome assembly of A. spinosa. A reliable reference genome of A. spinosa was created using a hybrid de novo assembly approach combining short and long sequencing reads. Results: In total, 144 Gb Illumina HiSeq reads and 7.2 Gb PacBio reads were produced and assembled. The final draft genome comprises 75 327 scaffolds totaling 671 Mb with an N50 of 49 916 kb. The draft assembly is close to the genome size estimated by k-mers distribution and covers 89% of complete and 4.3 % of partial Arabidopsis orthologous groups in BUSCO. Conclusion: The A. spinosa genome will be useful for assessing biodiversity leading to efficient conservation of this endangered endemic tree. Furthermore, the genome may enable genome-assisted cultivar breeding, and provide a better understanding of important metabolic pathways and their underlying genes for both cosmetic and pharmacological purposes

A Comprehensive Approach to Identify Reliable Reference Gene Candidates to Investigate the Link between Alcoholism and Endocrinology in Sprague-Dawley Rats

Gender and hormonal differences are often correlated with alcohol dependence and related complications like addiction and breast cancer. Estrogen (E2) is an important sex hormone because it serves as a key protein involved in organism level signaling pathways. Alcoholism has been reported to affect estrogen receptor signaling; however, identifying the players involved in such multi-faceted syndrome is complex and requires an interdisciplinary approach. In many situations, preliminary investigations included a straight forward, yet informative biotechniques such as gene expression analyses using quantitative real time PCR (qRT-PCR). The validity of qRT-PCR-based conclusions is affected by the choice of reliable internal controls. With this in mind, we compiled a list of 15 commonly used housekeeping genes (HKGs) as potential reference gene candidates in rat biological models. A comprehensive comparison among 5 statistical approaches (geNorm, dCt method, NormFinder, BestKeeper, and RefFinder) was performed to identify the minimal number as well the most stable reference genes required for reliable normalization in experimental rat groups that comprised sham operated (SO), ovariectomized rats in the absence (OVX) or presence of E2 (OVXE2). These rat groups were subdivided into subgroups that received alcohol in liquid diet or isocalroic control liquid diet for 12 weeks. Our results showed that U87, 5S rRNA, GAPDH, and U5a were the most reliable gene candidates for reference genes in heart and brain tissue. However, different gene stability ranking was specific for each tissue input combination. The present preliminary findings highlight the variability in reference gene rankings across different experimental conditions and analytic methods and constitute a fundamental step for gene expression assays