368 research outputs found
Isolation and Identification of an Antimutagenic Phthalate Derivative Compound from Octopus (Paraoctopus limaculatus)
Purpose: To isolate and evaluate the antimutagenic properties of compounds previously identified in octopus (Paraoctopus limaculatus).Methods: Octopus fractions, previously obtained by a sequential thin layer chromatography (TLC) procedure, were subjected to further fractionation by TLC and their anti-mutagenic activity monitored using Salmonella tester strains TA98 and TA100 with metabolic activation (S9) in Ames test. The isolated fractions were subjected to structural studies by Fourier transformed infrared spectroscopy (FTIR), nuclear magnetic resonance (1H and 13C NMR), and gas chromatography-mass spectrometry.Results: Five new fractions were obtained from a previously isolated and reported anti-mutagenic octopus fraction. Fractions RB21321b2 and RB21321b3 inhibited > 80 % of the mutagenicity induced by 500 ng AFB1 on both tester strains and were selected for chemical/structural characterization. Data from IR and 1H and 13C NMR suggested the presence of phthalate type of compounds. GC-MS analysis revealed 278 m/z for both fractions which is consistent with a butyl isobutyl phthalate structure.Conclusion: Based on the findings, the compound responsible for the high anti-mutagenic activity of the isolated fraction from octopus is 1-butyl-2-isobutyl-phthalate.Keywords: Octopus, Anti-Mutagenic, Paraoctopus limaculatus, 1-Butyl-2-isobutyl-phthalat
Lupane-type triterpenes and their anti-cancer activities against most common malignant tumors
In recent times, a great deal of interest has been motivated on plant derived compounds known as nutraceuticals. These compounds exert important beneficial activities that improve people’s health status when are consumed regularly, and now they appear as a viable option to explore their possible therapeutic effects against diseases like cancer. Particularly, lupane-type triterpenes have shown great ability to modulate multiple cancer-related signaling pathways and processes, including NF-κB, Wnt/β-catenin, PI3K/Akt, apoptosis, and many other routes related to proliferation or cell death, which are uncontrolled in malignant tumors. These investigations have promoted in vitro and in vivo studies, searching their mechanisms of action; although more research is still needed to prove its potential in human clinical trials. This review focuses on the ability of betulin, betulinic acid and lupeol to show benefits against the most common types of malignant tumors, which are considered a major global threat for public health
Condition-dependent male copulatory courtship and its benefits for females
Postcopulatory sexual selection has shaped the ornaments used during copulatory courtship. However, we know relatively little about whether these courtship ornaments are costly to produce or whether they provide indirect benefits to females. We used the mealworm beetle, Tenebrio molitor, to explore this. We challenged males using an entomopathogenic fungus and compared their courtship (frequency of leg and antennal contacts to the female), copulation duration, number of eggs laid, and hatching rate against control males. Infected males copulated for longer yet they reduced their leg and antennal contacts compared to control males. However, there was no obvious relation between infection, copulation duration, and courtship with egg production and hatching success. In general, our results indicate that the ornaments used during postcopulatory courtship are condition-dependent. Moreover, such condition dependence cannot be linked to male fitness.Fil: Cargnelutti, Franco Ignacio. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Diversidad y EcologĂa Animal. Universidad Nacional de CĂłrdoba. Facultad de Ciencias Exactas FĂsicas y Naturales. Instituto de Diversidad y EcologĂa Animal; ArgentinaFil: Reyes RamĂrez, Alicia. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: Cristancho, Shara. Universidad El Bosque; ColombiaFil: Sandoval GarcĂa, Iván A.. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: Rocha Ortega, Maya. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xicoFil: Calbacho Rosa, LucĂa Soledad. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Diversidad y EcologĂa Animal. Universidad Nacional de CĂłrdoba. Facultad de Ciencias Exactas FĂsicas y Naturales. Instituto de Diversidad y EcologĂa Animal; ArgentinaFil: Palacino, Freddy. Universidad El Bosque; ColombiaFil: CĂłrdoba Aguilar, Alex. Universidad Nacional AutĂłnoma de MĂ©xico; MĂ©xic
WIYN Open Cluster Study. XXXIX. Abundances in NGC 6253 from HYDRA Spectroscopy of the Li 6708 A Region
High-dispersion spectra of 89 potential members of the old, super-metal-rich
open cluster, NGC 6253, have been obtained with the HYDRA multi-object
spectrograph. Based upon radial-velocity measurements alone, 47 stars at the
turnoff of the cluster color-magnitude diagram (CMD) and 18 giants are
identified as potential members. Five turnoff stars exhibit evidence of
binarity while proper-motion data eliminates two of the dwarfs as members. The
mean cluster radial velocity from probable single-star members is -29.4 +/- 1.3
km/sec (sd). A discussion of the current estimates for the cluster reddening,
derived independently of potential issues with the BV cluster photometry, lead
to an adopted reddening of E(B-V) = 0.22 +/- 0.04. From equivalent width
analyses of 38 probable single-star members near the CMD turnoff, the weighted
average abundances are found to be [Fe/H] = +0.43 +/- 0.01, [Ni/H] = +0.53 +/-
0.02 and [Si/H] = +0.43 (+0.03,-0.04), where the errors refer to the standard
errors of the weighted mean. Weak evidence is found for a possible decline in
metallicity with increasing luminosity among stars at the turnoff. We discuss
the possibility that our turnoff stars have been affected by microscopic
diffusion. For 15 probable single-star members among the giants, spectrum
synthesis leads to abundances of +0.46 (+0.02,-0.03) for [Fe/H]. While less
than half the age of NGC 6791, NGC 6253 is at least as metal-rich and, within
the uncertainties, exhibits the same general abundance pattern as that typified
by super-metal-rich dwarfs of the galactic bulge.Comment: 5 Tables, 9 figures, 45 page
Intracellular calcium movements of boar spermatozoa during 'in vitro' capacitation and subsequent acrosome exocytosis follow a multiple-storage place, extracellular calcium-dependent model
This work analysed intracellular calcium stores of boar spermatozoa subjected to invitro' capacitation (IVC) and subsequent progesterone-induced acrosome exocytosis (IVAE). Intracellular calcium was analysed through two calcium markers with different physico-chemical properties, Fluo-3 and Rhod-5N. Indicative parameters of IVC and IVAE were also evaluated. Fluo-3 was located at both the midpiece and the whole head. Rhod-5N was present at the sperm head. This distribution did not change in any of the assayed conditions. Induction of IVC was concomitant with an increase in both head and midpiece Ca2+ signals. Additionally, while IVC induction was concurrent with a significant (p<0.05) increase in sperm membrane permeability, no significant changes were observed in O-2 consumption and ATP levels. Incubation of boar spermatozoa in the absence of calcium showed a loss of both Ca2+ labellings concomitantly with the sperm's inability to achieve IVC. The absence of extracellular calcium also induced a severe decrease in the percentage of spermatozoa exhibiting high mitochondrial membrane potential (hMMP). The IVAE was accompanied by a fast increase in both Ca2+ signalling in control spermatozoa. These peaks were either not detected or much lessened in the absence of calcium. Remarkably, Fluo-3 marking at the midpiece increased after progesterone addition to sperm cells incubated in a medium without Ca2+. The simultaneous addition of progesterone with the calcium chelant EGTA inhibited IVAE, and this was accompanied by a significant (p<0.05) decrease in the intensity of progesterone Ca2+-induced peak, O-2 consumption and ATP levels. Our results suggest that boar spermatozoa present different calcium deposits with a dynamic equilibrium among them and with the extracellular environment. Additionally, the modulation role of the intracellular calcium in spermatozoa function seems to rely on its precise localization in boar spermatozoa
The herpetofauna of Veracruz, Mexico: composition, distribution, and conservation status
The herpetofauna of the state of Veracruz, Mexico, currently consists of 359 species, including 76 anurans, 45 caudates, one caecilian, one crocodylian, 217 squamates, and 19 turtles. The distribution of the herpetofaunal species are catalogued here among the four recognized physiographic regions in the state. The total number of species ranges from 179 in the Sierra de Los Tuxtlas to 236 in the Sierra Madre Oriental. The number of species shared among the four physiographic regions ranges from 100 between the Gulf Coastal Lowlands and the Transmexican Volcanic Belt, to 190 between the Sierra Madre Oriental and the Transmexican Volcanic Belt. A similarity dendrogram based on the Unweighted Pair Group Method with Arithmetic Averages (UPGMA) depicts two distinct clusters, one between the Sierra Madre Oriental and the Transmexican Volcanic Belt, and the other between the Gulf Coastal Lowlands and the Sierra de Los Tuxtlas. The former cluster refects two adjacent regions in highland environments that share a substantial number of herpetofaunal species, and the latter cluster shares a sizeable number of wide-ranging, generalist, lowland species found on the Atlantic and Pacifc versants of Mexico and Central America. The level of herpetofaunal endemism is relatively high, with 182 of 359 species either endemic to Mexico or to Veracruz. The distributional categorization of the total herpetofauna is as follows: 169 non-endemic species; 138 country endemic species; 44 state endemic species; and eight non-native species. The 169 non-endemic species are allocated to the following distributional categories: MXCA (89), MXSA (30), MXUS (29), USCA (11), USSA (four), and OCEA (fve). The principal environmental threats to the herpetofauna of Veracruz include deforestation, livestock, roads, water pollution, myths and other cultural factors, diseases, invasive species, and illegal commerce. The conservation status of each native species was evaluated using the SEMARNAT, IUCN, and EVS systems, of which the EVS system proved to be the most useful. The Relative Herpetofaunal Priority method was employed to determine the rank order signifcance of the four regions, and this identifed the Sierra Madre Oriental as the region of greatest importance. Only six protected areas exist in Veracruz, most of which are located in the Gulf Coastal Lowlands, the region of least conservation signifcance. The area of greatest signifcance, the Sierra Madre Oriental, does not contain any protected areas. A total of 265 species have been recorded within the six protected areas, of which 138 are non-endemics, 89 are country endemics, 31 are state endemics, and seven are non-natives. Finally, we provide a set of conclusions and recommendations to enhance the prospects for the future protection of the herpetofauna of Veracruz
Carbon Fiber Composites of Pure Polypropylene and Maleated Polypropylene Blends Obtained from Injection and Compression Moulding
A comparative study of the mechanical performance of PP and PP/PP-g-MAH blends reinforced with carbon fibre (CF) obtained by two different moulding techniques is presented. Three filler contents were used for fabricating the composites: 1, 3, and 5 pph (parts per hundred). The crystallisation behaviour of the composites was studied by differential scanning calorimetry. Morphological and structural features of these samples were observed by atomic field microscopy and Fourier-transform infrared spectroscopy, respectively. Mechanical properties of the injection and compression moulded composites were evaluated by means of tensile and impact resistance tests. The fracture surface of the impacted samples was observed by scanning electron microscopy. The processing method had a noticeable effect on the results obtained in these tests. Young’s modulus was enhanced up to 147% when adding 5 pph CF to a PP matrix when processed by compression moulding. Addition of PP-g-MAH and CF had a favourable effect on the tensile and impact strength properties in most samples; these composites showed improved performance as the filler content was increased
Atypical AT Skew in Firmicute Genomes Results from Selection and Not from Mutation
The second parity rule states that, if there is no bias in mutation or selection, then within each strand of DNA complementary bases are present at approximately equal frequencies. In bacteria, however, there is commonly an excess of G (over C) and, to a lesser extent, T (over A) in the replicatory leading strand. The low G+C Firmicutes, such as Staphylococcus aureus, are unusual in displaying an excess of A over T on the leading strand. As mutation has been established as a major force in the generation of such skews across various bacterial taxa, this anomaly has been assumed to reflect unusual mutation biases in Firmicute genomes. Here we show that this is not the case and that mutation bias does not explain the atypical AT skew seen in S. aureus. First, recently arisen intergenic SNPs predict the classical replication-derived equilibrium enrichment of T relative to A, contrary to what is observed. Second, sites predicted to be under weak purifying selection display only weak AT skew. Third, AT skew is primarily associated with largely non-synonymous first and second codon sites and is seen with respect to their sense direction, not which replicating strand they lie on. The atypical AT skew we show to be a consequence of the strong bias for genes to be co-oriented with the replicating fork, coupled with the selective avoidance of both stop codons and costly amino acids, which tend to have T-rich codons. That intergenic sequence has more A than T, while at mutational equilibrium a preponderance of T is expected, points to a possible further unresolved selective source of skew
Two chemically similar stellar overdensities on opposite sides of the plane of the Galaxy
Our Galaxy is thought to have undergone an active evolutionary history
dominated by star formation, the accretion of cold gas, and, in particular,
mergers up to 10 gigayear ago. The stellar halo reveals rich fossil evidence of
these interactions in the form of stellar streams, substructures, and
chemically distinct stellar components. The impact of dwarf galaxy mergers on
the content and morphology of the Galactic disk is still being explored. Recent
studies have identified kinematically distinct stellar substructures and moving
groups, which may have extragalactic origin. However, there is mounting
evidence that stellar overdensities at the outer disk/halo interface could have
been caused by the interaction of a dwarf galaxy with the disk. Here we report
detailed spectroscopic analysis of 14 stars drawn from two stellar
overdensities, each lying about 5 kiloparsecs above and below the Galactic
plane - locations suggestive of association with the stellar halo. However, we
find that the chemical compositions of these stars are almost identical, both
within and between these groups, and closely match the abundance patterns of
the Milky Way disk stars. This study hence provides compelling evidence that
these stars originate from the disk and the overdensities they are part of were
created by tidal interactions of the disk with passing or merging dwarf
galaxies.Comment: accepted for publication in Natur
Identification of red high proper-motion objects in Tycho-2 and 2MASS catalogues using Virtual Observatory tools
Aims: With available Virtual Observatory tools, we looked for new M dwarfs in
the solar neighbourhood and M giants with high tangential velocities. Methods:
From an all-sky cross-match between the optical Tycho-2 and the near-infrared
2MASS catalogues, we selected objects with proper motions >50mas/yr and very
red V-Ks colours. For the most interesting targets, we collected
multi-wavelength photometry, constructed spectral energy distributions,
estimated effective temperatures and surface gravities from fits to atmospheric
models, performed time-series analysis of ASAS V-band light curves, and
assigned spectral types from low-resolution spectroscopy obtained with CAFOS at
the 2.2m Calar Alto telescope. Results: We got a sample of 59 bright red high
proper-motion objects, including fifty red giants, four red dwarfs, and five
objects reported in this work for the first time. The five new stars have
magnitudes V~10.8-11.3mag, reduced proper motions midway between known dwarfs
and giants, near-infrared colours typical of giants, and effective temperatures
Teff~2900-3400K. From our time-series analysis, we discovered a long secondary
period in Ruber 4 and an extremely long primary period in Ruber 6. With the
CAFOS spectra, we confirmed the red giant nature of Ruber 7 and 8, the last of
which seems to be one of the brightest metal-poor M giants ever identified.Comment: Accepted in Astronomy & Astrophysic
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