Microwave energy supplied by a prototype oven prevents the spread of Fusarium wilt during the propagation of melon plantlets by seed

The re-use of propagation trays in nursery greenhouses is one of the main ways in which fusarium wilt is spread in melon crops (Cucumis melo). The causal agent of the disease is the fungus Fusarium oxysporum f. sp. melonis. This paper reports that exposing these seed trays to the energy produced by a prototype microwave oven during the commercial production of melon plantlets can prevent the spread of this pathogen with only a very small increase in production costs

Mejora de la tecnología de la pulverización de productos fitosanitarios sobre plantaciones de vid en espaldera

Las muy variadas formas de penetración de los hongos aéreos en las plantas cultivadas, obligan a pensar que el ideal de las aplicaciones fitosanitarias, sobre todo cuando de fungicidas de contacto se trata, es la formación de una fina película de producto fitosanitario sobre toda la superficie vegetal. La cualificación del porcentaje de recubrimiento logrado, que define la calidad de un tratamiento, es de gran importancia, pues de dicho porcentaje depende en gran parte su eficacia. La forma de cuantificar el recubrimiento de la superficie vegetal conseguido es muy subjetiva, es por lo que establecer criterios objetivos que permitan cuantificar la calidad de distribución de los tratamientos fitosanitarios sobre las plantas, puede permitir, además de mejorar la eficacia de los productos, incidir en las características de utilización y de diseño de las máquinas usadas en la protección de cultivos. Con este trabajo de tesis doctoral se ha pretendido hacer aportaciones que permitan, de forma objetiva, clara y práctica, mejorar la calidad de las aplicaciones fitosanitarias y permitir su cuantificación en viñedo en espaldera. Los resultados de los trabajos realizados, altamente satisfactorios, han abierto nuevos horizontes tanto en la protección fitosanitaria de la vid como en otras plantas cultivadas

Microsurgery in complex trauma of pelvic limb in a pediatric patient: case report

Complex trauma is defined as the condition secondary to the exchange of kinetic energy of two or more tissues in one limb. This entity is a surgical emergency that can have many sequelae and can even result in limb loss. An 11-year-old female patient presents complex pelvic limb trauma secondary to contuse injury caused by a helicopter’s rotor blades.  Pelvic limb reconstruction was performed with iliac crest bone graft, the fracture was stabilized with an external fixator and the skin defect was covered with an anterolateral microvascular thigh flap (ALT). There was an adequate integration of the bone graft with adequate skin coverage thanks to the ALT thigh flap. The patient presented discreet limb shortening as consequence.  Currently, microsurgery is the only medical option that meets the objectives of limb reconstruction. Microsurgical techniques can be used in pediatric and adult patients. The success of any recovery from complex trauma is vigorous surgical cleaning, avoiding sequential and/or multiple washes

Effects of Enriched Physical and Social Environments on Motor Performance, Associative Learning, and Hippocampal Neurogenesis in Mice

We have studied the motor abilities and associative learning capabilities of adult mice placed in different enriched environments. Three-month-old animals were maintained for a month alone (AL), alone in a physically enriched environment (PHY), and, finally, in groups in the absence (SO) or presence (SOPHY) of an enriched environment. The animals' capabilities were subsequently checked in the rotarod test, and for classical and instrumental learning. The PHY and SOPHY groups presented better performances in the rotarod test and in the acquisition of the instrumental learning task. In contrast, no significant differences between groups were observed for classical eyeblink conditioning. The four groups presented similar increases in the strength of field EPSPs (fEPSPs) evoked at the hippocampal CA3-CA1 synapse across classical conditioning sessions, with no significant differences between groups. These trained animals were pulse-injected with bromodeoxyuridine (BrdU) to determine hippocampal neurogenesis. No significant differences were found in the number of NeuN/BrdU double-labeled neurons. We repeated the same BrdU study in one-month-old mice raised for an additional month in the above-mentioned four different environments. These animals were not submitted to rotarod or conditioned tests. Non-trained PHY and SOPHY groups presented more neurogenesis than the other two groups. Thus, neurogenesis seems to be related to physical enrichment at early ages, but not to learning acquisition in adult mice

RICORS2040 : The need for collaborative research in chronic kidney disease

Chronic kidney disease (CKD) is a silent and poorly known killer. The current concept of CKD is relatively young and uptake by the public, physicians and health authorities is not widespread. Physicians still confuse CKD with chronic kidney insufficiency or failure. For the wider public and health authorities, CKD evokes kidney replacement therapy (KRT). In Spain, the prevalence of KRT is 0.13%. Thus health authorities may consider CKD a non-issue: very few persons eventually need KRT and, for those in whom kidneys fail, the problem is 'solved' by dialysis or kidney transplantation. However, KRT is the tip of the iceberg in the burden of CKD. The main burden of CKD is accelerated ageing and premature death. The cut-off points for kidney function and kidney damage indexes that define CKD also mark an increased risk for all-cause premature death. CKD is the most prevalent risk factor for lethal coronavirus disease 2019 (COVID-19) and the factor that most increases the risk of death in COVID-19, after old age. Men and women undergoing KRT still have an annual mortality that is 10- to 100-fold higher than similar-age peers, and life expectancy is shortened by ~40 years for young persons on dialysis and by 15 years for young persons with a functioning kidney graft. CKD is expected to become the fifth greatest global cause of death by 2040 and the second greatest cause of death in Spain before the end of the century, a time when one in four Spaniards will have CKD. However, by 2022, CKD will become the only top-15 global predicted cause of death that is not supported by a dedicated well-funded Centres for Biomedical Research (CIBER) network structure in Spain. Realizing the underestimation of the CKD burden of disease by health authorities, the Decade of the Kidney initiative for 2020-2030 was launched by the American Association of Kidney Patients and the European Kidney Health Alliance. Leading Spanish kidney researchers grouped in the kidney collaborative research network Red de Investigación Renal have now applied for the Redes de Investigación Cooperativa Orientadas a Resultados en Salud (RICORS) call for collaborative research in Spain with the support of the Spanish Society of Nephrology, Federación Nacional de Asociaciones para la Lucha Contra las Enfermedades del Riñón and ONT: RICORS2040 aims to prevent the dire predictions for the global 2040 burden of CKD from becoming true

Mycorrhizas in South American Anthropic Environments

The agricultural expansion has leaded to increase the irrigated cropland area and the use of fertilizers, resulting in water degradation, increased energy use, and common pollution. Of particular concern is the increased interest to reduce the environmental impacts of high quantities of water dedicated to irrigation by agricultural activities We are now truly recognizing the importance of sustainable measures in agriculture such as conservation of the vegetation cover and management approach to understand surface and deep soil responses to global change. The agroecology management based on key processes from natural ecosystems can help to solve some agricultural difficulties. Increasing studies on the Arbuscular mycorrhizal fungi (AMF) has showed their importance for soil ecology and studies on their biodiversity have spread in some agro-ecosystems such as corn and soybean monocultures. Therefore, it is needed to deeply study the mycorrhizal functions under global change. In this chapter, we examine the major developments and advances on mycorrhizal fungi based on recent research from South American countries. New reports on the occurrence of mycorrhizas in Amazonian dark earth, as well as the inoculum production of arbuscular mycorrhizal fungi native of soils under native forest covers, have resulted in a more detailed understanding of the soil biology from South America. Reports from Amazonian dark earth or “Terra preta do índio” soil has stimulated the use of biochar worldwide as a soil conditioner that can add value to non-harvested agricultural products and promote plant growth. Few reports from Brazil showed that the addition of inorganic fertilizer, compost and chicken manure resulted in increases in plant cover and plant species richness. In this sense, the biochar/mycorrhizae interactions also can be prioritized for sequestration of carbon in soils to contribute to climate change mitigation

Comparación del recubrimiento con productos fitosanitarios alcanzado en viña en espaldera por un prototipo de pulverizador tipo túnel, un pulverizador, un atomizador y un nebulizador

Spraying is the main method used to apply pesticides to trellised vines in Castilla-La Mancha, Spain. If the efficiency of spray applications is to be improved, the amount of pesticide employed is to be reduced, and the performance of existing and new spray technology enhanced, the leaf coverage achieved must be known and a system developed that can rapidly determine it. With these aims in mind, the authors built and tested a prototype tunnel sprayer and compared its efficiency to the three types of sprayer most commonly used in the region: the hydraulic sprayer, the air-assisted sprayer and the pneumatic sprayer. To determine and compare the coverage produced by these four machines, a rapid analysis system that combines the use of water-sensitive paper strips and an artificial vision system was developed. All four machines were used to spray a commercial fungicide (dose 200 L haE-1) at working pressures of 0.1, 0.3 and 0.6 MPa onto vines in a trellised vineyard. With all four models, the quality of the application improved with the spraying pressure, although the best spraying coverage was obtained with tunnel sprayer. Even though the results obtained were not optimum (the formation of a uniform film of fungicide on the leaf surface), the mean coverage achieved by the tunnel sprayer (as recorded by the water-sensitive papers attached to the leaves) was 54%, and on occasion over 79%.La pulverización es el principal método usado en Castilla-La Mancha para aplicar productos fitosanitarios a la viña en espaldera. Si la eficiencia de las aplicaciones se incrementa, la cantidad de pesticida empleado se reduce y el rendimiento de las máquinas existentes y de las nuevas tecnologías aumenta. Para ello, debe conocerse el recubrimiento alcanzado en las hojas y desarrollarse un sistema que pueda determinarlo rápidamente. Con estos objetivos se construyó y ensayó un prototipo de pulverizador tipo túnel, y se comparó su eficiencia con los tres tipos de máquinas de aplicación de productos fitosanitarios más utilizados en esta región: pulverizadores, atomizadores y nebulizadores. Para determinar el porcentaje de recubrimiento logrado por las máquinas, se desarrolló un sistema rápido que combina el uso de cartulinas de papel hidrosensible con la visión artificial. Con todos los pulverizadores se usó el mismo fungicida comercial (200 L haE-1) y se ensayaron, a tres presiones diferentes (0,1 MPa, 0,3 MPa y 0,6 MPa), en un viñedo en espaldera. Se demostró que en los cuatro modelos la calidad de la aplicación mejora con la presión de la pulverización, y que el mejor recubrimiento se obtuvo con el prototipo de pulverizador tipo túnel. Aunque los resultados obtenidos no fueron óptimos (formación de una película uniforme de fungicida), el valor medio alcanzado por el prototipo fue del 54%, y en ocasiones superó el 79%