58 research outputs found
Helicobacter pullorum cytolethal distending toxin targets vinculin and cortactin and triggers formation of lamellipodia in intestinal epithelial cells
Helicobacter pullorum, a bacterium initially isolated from poultry, has been associated with human digestive
disorders. However, the factor responsible for its cytopathogenic effects on epithelial cells has not been formally
identified. The cytopathogenic alterations induced by several human and avian H. pullorum strains were investigated
on human intestinal epithelial cell lines. Moreover, the effects of the cytolethal distending toxin
(CDT) were evaluated first by using a wild-type strain and its corresponding cdtB isogenic mutant and second
by delivering the active CdtB subunit of the CDT directly into the cells. All of the H. pullorum strains induced
cellular distending phenotype, actin cytoskeleton remodeling, and G2/M cell cycle arrest. These effects were dependent
on the CDT, as they were (1) not observed in response to a cdtB isogenic mutant strain and (2) present
in cells expressing CdtB. CdtB also induced an atypical delocalization of vinculin from focal adhesions to the
perinuclear region, formation of cortical actin-rich large lamellipodia with an upregulation of cortactin, and
decreased cellular adherence. In conclusion, the CDT of H. pullorum is responsible for major cytopathogenic
effects in vitro, confirming its role as a main virulence factor of this emerging human pathogen.This work was supported by the Institut national de la santĂ© et de la recherche mĂ©dicale, the University Bordeaux Segalen, the Conseil RĂ©gional dâAquitaine (grants 20030304002FA and 20040305003 FA), the SociĂ©tĂ© Nationale Française de GastroentĂ©rologie, the European Union (FEDER no. 2003227
The NWCSAF/GEO software package for the MSG/IODC satellite service
PĂłster presentado en: EUMETSAT Meteorological Satellite Conference celebrada del 2 al 6 de Octubre de 2017 en Roma
The nowcasting SAF products and services: recent improvements in the new SW packages PPS v2018 and GEO v2018 and future plans
PresentaciĂłn realizada en la 3rd European Nowcasting Conference, celebrada en la sede central de AEMET en Madrid del 24 al 26 de abril de 2019
Use of NWCSAF NWC/GEO Software Package with MSG, HIMAWARI-8/9 and GOES-13/16 Satellites
Ponencias presentadas en: Joint EUMETSAT/AMS/NOAA Conference 2019 celebrada en Boston del 30 de septiembre al 4 de octubre de 2019
Regards sur l'archéologie dans la Loire: Journées d'études tenues les 6 et 7 novembre 2009 au Musée Déchelette de Roanne. Histoire et Patrimoine de Roanne et sa région, n°17
Numéro spécial : parution des communications des Journées d'études tenues les 6 et 7 novembre 2009 au Musée Déchelette de Roanne à l'occasion du 50Úme anniversaire du Groupe de recherches archéologiques du Roannais
Dispersion improvement of carbon nanotubes in epoxy resin using amphiphilic block copolymers
International audienceInterface between Carbon NanoTubes (CNT) and epoxy matrix is admitted to play an important role in the dispersion quality and in the mechanical stress transfer. To improve the interfacial adhesion, we propose to chemically graft molecules onto CNT surface. To achieve this chemical modification, a controlled radical polymerization, named Nitroxide Mediated Polymerization NMP, is used to synthesize a diblock copolymer based on Acrylic Acid (PAA block) and Methyl MethAcrylate (PMMA block). In the present paper, this polymerization is performed "in situ ". The PAA block presents a good affinity with the CNT which enable grafting. The PMMA miscibility with epoxy is expected to give a good adhesion-between the CNT and the matrix-and to bring a better dispersion. In order to compare the chemical modification and the physical adsorption of the copolymers onto CNT dispersion, the same block copolymer was synthesized with and without CNT. The copolymer synthesis was controlled and characterized by different methods as NMR 1H (conversion and composition), SEC (molecular weight) and TGA (grafting density). We show that the better dispersion quality and better physical properties have been obtained with grafted CNT
Endommagement et anomalie thermique liés à la propagation de failles: exemples des marnes de Pampelune (Sigues, sud Pyrénées)
ACTInternational audienc
Compatibility of Cloxacillin Sodium with Selected Intravenous Drugs during Simulated Y-Site Administration
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