Involvement of intact inositol-1,4,5-trisphosphate-sensitive Ca2+ stores in cell cycle progression at the G1/S boundary in serum-stimulated human fibroblasts

AbstractThapsigargin, a selective inhibitor of the endoplasmic reticulum Ca2+ pump, has been shown to deplete inositol-1,4,5-trisphosphate-sensitive Ca2+ stores. Here we report that when thapsigargin was introduced to serum-stimulated human fibroblasts at a time point just before the G1/S boundary, it completely inhibited expression of cyclin A, activation of p33CDK2 cyclindependent kinase and initiation of DNA synthesis. In contrast, the Ca2+ mobilizing ionophore ionomycin was without effect. These findings indicate that Ca2+ inside the inositol-1,4,5-trisphosphatesensitive Ca2+ stores plays a pivotal role for traverse across the G1/S transition point

Hybrid organometallo-silica catalysts for sustainable visible-light promoted olefin isomerization

The use of visible light as a clean and affordable reagent has turned photocatalysis into a green and powerful tool in synthetic chemistry. However, most of these photocatalytic processes are carried out under homogeneous conditions, which requires expensive catalysts that are difficult to recover and recycle. One way to overcome this problem is to incorporate the visible-active species into porous solid matrices. However, integrating these active species into the matrix to achieve a stable and active heterogeneous photocatalyst is not easy, and has usually been done by post-synthetic strategies. Herein, we describe the use of the highly emissive cyclometalated complex [Ir(dfppy)2(dasipy)]PF6 as a building block for the in-situ synthesis of four different silica-based heterogeneous photocatalysts, and a related post-synthetic grafted material. These highly stable materials have been assessed in the energy transfer photo-isomerization reaction of trans- to cis-stilbene under blue light and mild conditions; showing not only high isomerization yields (ca. 80 %), but also easy recovery and excellent recyclability (up to 100 % after 7 cycles).This work was supported by the Spanish MCIN/AIE/10.13039/501100011033, the ‘‘ERDF A way of making Europe’’, the ‘‘European Union’’ (projects PID2019-109742GB-I00 and PID2021-128761OB-C21), Gobierno de la Rioja (Project FORTALECE 2021/01) and Generalitat Valenciana (Regional Project AICO/2021/037 - Conselleria d’Innovació, Universitats, Ciència i Societat Digital)

Scalar fields in an anisotropic closed universe

We study in this article a class of homogeneous, but anisotropic cosmological models in which shear viscosity is included. Within the matter content we consider a component (the quintessence component) determined by the barotropic equations of state, $p=\alpha \rho$, with $\alpha < 0$. We establish conditions under which a closed axisymmetrical cosmological model may look flat al low redshift.Comment: 6 pages, Latex, 2 figures, accepted in Phys. Rev.

Behaviour of uranium along Jucar River (Eastern Spain) Determination of 234U/238U and 235U/238U ratios

The uranium concentration and the U-234/U-238, U-235/U-238 activity ratios were studied in water samples from Jucar River, using low-level alpha-spectrometry. The effects of pH, temperature and salinity were considered and more detailed sampling was done in the neighbourhood of Cofrentes Nuclear plant (Valencia, Spain). Changes were observed in the uranium concentration with the salinity and the U-234/U-238 activity ratio was found to vary with pH. Leaching and dilution, which depend on pH and salinity, are the probable mechanisms for these changes in the concentration of uranium and the activity ratios.Rodríguez Álvarez, MJ.; Sanchez, F. (1995). Behaviour of uranium along Jucar River (Eastern Spain) Determination of 234U/238U and 235U/238U ratios. Journal of Radioanalytical and Nuclear Chemistry. 190(1):113-120. doi:10.1007/BF02035642S1131201901M. J. RORDÍGUEZ-AALVAREZ, F. SÁNCHEZ, E. NAVARRO, Proc. 3nd Intern. Summer School, Huelva Spain, M. GARCÍA-LEÓN, G. MADURGA (Eds), World Scientific, Singapore, 1994.M. IVANOVICH, R. S. HARMON (Eds), Uranium Disequilibrium Series: Applications to Environmental Problems, Clarendon Press, Oxford, 1982.K. OSMOND, J. B. COWART, At. Energy Rev., 14 (1976) 621.B. L. DICKSON, R. L. MEAKINS, Nucl. Instrum. Methods Phys. Res., 223 (1983) 593.J. L. GASCÓN, MURILLO, PhD Thesis, University of Zaragoza, Spain, 1990.M. C. MORÓN, A. MARTINEZ-AGUIRRE, M. GARCÍA-LEÓN, Intern. Conf. on Environmental Radioactivity in the Mediterranean Area, Barcelona 10–13 May 1988, SNE-ENS, Barcelona, 1988, p. 111.R. BOJANOWSKI, R. FUKAI, S. BALLESTRA, H. ASARI, Determination of natural radioactive elements in marine environmental materials by ion-exchange and α-spectrometry. Proc. 4th Symp. on the Determination of Radionuclides in Environmental and Biological Materials, April 1983, London, Ed-Rd Press.R. GARCÍA-TENORIO, M. GARCÍA-LEÓN, G. PIAZZA, Anal. Física B, 82 (1986) 238.L. HALLSTADIUS, Nucl. Instrum. Methods Phys. Res., 223 (1984) 266.F. VERA, A. MARTIN, Nucl. Instrum. Methods Phys. Res., A276 (1989) 289.A. MARTINEZ-AGUIRRE, M. GARCÍA-LEÓN, J. Radioanal. Nucl. Chem., 155 (1991) 97.J. R. DOOLEY, H. C. ROSHOLT, Econ. Geol., 61 (1996) 326.A. MARTINEZ-AGUIRRE, M. GARCÍA-LEÓN, Radiat. Prot. Dosim., 45 (1992) 249.J. TOOLE, M. S. BAXTER, J. THOMSON, Estuarine, Coastal and Shelf Sci., 25 (1987) 283.S. G. BHAT, S. KRISHANASWAMI, Proc. Indian Acad. Sci., A LXX, (1969).K. K. TUREKIAN, J. K. COCHRAN, in: Chemical Oceanography, Vol. 7, J. P. RILEY and R. CHESTER (Eds), 2nd ed., Academic Press, New York, 1978.D. LANGMUIR, Geochim. Cosmochim. Acta, 42 (1978) 547.J. M. MARTIN, M. MEYBECK, Mar. Chem., 7 (1979) 173.Radionuclide Transformations, Annals of the ICRP, ICRP Publication 38, Vol. 11–13, Pergamon Press, 1983.A. MANGINI, G. SONNTAG, G. BERTSCH, E. MÜLLER, Nature, 278 (1979) 337.M. R. SCOTT, in: M. IVANOVICH, R. S. HARMON (Eds), Uranium Disequilibrium Series: Applications to Environmental Problems, Clarendon Press, Oxford, 1982.J. K. OSMOND, J. B. COWART, in: M. IVANOVICH, R. S. HARMON (Eds), Uranium Disequilibrium Series: Applications to Environmental Problems, Clarendon Press, Oxford, 1982.R. BOWEN (Eds), Isotopes in the Earth Sciences, Elsevier Applied Science, 1988.F. VERA, PhD Thesis, University Extremadura, Spain, 1988.F. VERA, A. MARTIN, J. Radioanal. Nucl. Chem., 134 (1988) 73

Central and peripheral arterial stiffness responses to uninterrupted prolonged sitting combined with a high-fat meal: a randomized, controlled cross-over trial

Background and aims: Independently, prolonged uninterrupted sitting and the consumption of a meal high in saturated fats acutely disrupt normal cardiovascular function. Currently the acute effects of these behaviours performed in combination on arterial stiffness, a marker of cardiovascular health, is unknown. This study sought to determine the effect of consuming a high-fat meal (Δ= 51 g fat) in conjunction with prolonged uninterrupted sitting (180 min) on measures of central and peripheral arterial stiffness. Methods: Using a randomized crossover design, thirteen young healthy males consumed a high-fat (61 g) or low-fat (10 g) meal before 180 min of uninterrupted sitting. Carotid-femoral (cf-) and femoral-ankle (fa-) pulse wave velocity (PWV), aortic-femoral stiffness gradient (af-SG), superficial femoral PWV beta (β), and oscillometric pulse wave analysis outcomes were assessed pre and post sitting. Results: cfPWV increased significantly more following the high-fat (mean difference [MD]= 0.59 m·s-1) when compared to the low-fat (MD= 0.2 m·s-1) meal, with no change in faPWV in either condition. The af-SG significantly decreased (worsened) (ηp2= 0.569) overtime in high and low-fat conditions (ratio= 0.1 and 0.1 respectively). Superficial femoral PWVβ significantly increased over time in high- and low-fat conditions (ηp2= 0.321; 0.8 and 0.4 m·s-1 respectively). A significant interaction found that triglycerides increased over time in the high fat trial only (ηp2= 0.761). There were no significant changes in blood pressures. Conclusions: Consuming a high-fat meal prior to 180 min of uninterrupted sitting augments markers of cardiovascular disease risk more than sitting following a low-fat meal

Comportamiento mecánico en función de la temperatura de aleaciones wolframio-vanadio.

En esta investigación se evalúa el comportamiento mecánico del wolframio puro y de dos de sus aleaciones con contenidos de 2% y 4% de vanadio, fabricadas mediante prensado isostático en caliente (HIP). La caracterización mecánica se ha realizado mediante ensayos de flexión en tres puntos en atmósfera oxidante a distintas temperaturas comprendidas entre temperatura ambiente y 1000 ºC. Adicionalmente, se han realizado ensayos en inmersión en nitrógeno líquido para los de -197 ºC. Estos últimos valores pueden servir para una determinación más precisa de la temperatura la transición dúctil-frágil en éstos materiales, y de su posible comportamiento plástico o frágil a temperatura ambiente. Mediante estos ensayos ha sido posible obtener la tenacidad de fractura, la resistencia mecánica, el límite elástico y el módulo de elasticidad en función de la temperatura. Así mismo, se ha determinado la densidad y la dureza Vickers para dos cargas distintas, lo que permitido determinar la influencia de este parámetro en la medida realizada. Adicionalmente, se han examinado mediante microscopia electrónica de barrido las superficies de fractura. De esta forma ha sido posible determinar el modo de rotura y analizar la relación de las propiedades mecánicas macroscópicas con los micromecanismos de fallo involucrados

mcr-Colistin resistance genes mobilized by IncX4, IncHI2, and IncI2 plasmids in Escherichia coli of pigs and white stork in Spain

Colistin has become the last-line antimicrobial for the treatment of multidrug resistant (MDR) Enterobacterales in human medicine. To date, several colistin resistance genes have been described. Of them mcr-1 is disseminated worldwide in Escherichia coli of human and animal origin. The aim of this study was to characterize mcr-mediated resistance plasmids from E. coli of animal origin in Spain. From our strain collection, 70 E. coli of pig origin collected between 2005 and 2014 (10 per year, except for years 2009-2010-2013) were randomly selected and screened for the presence of mcr-genes. Additionally, 20 E. coli isolated in 2011 from white storks (Ciconia ciconia) from the same urban household waste landfill associated colony were also included. Whole genome sequencing of mcr-positive isolates was carried out on a MiSeq (Illumina). Hybrid whole genome sequencing strategy combining nanopore and Illumina technologies were performed in a selection of isolates to close the genomes and plasmids and identify the presence of antimicrobial resistance genes. Minimum inhibitory concentration (MIC) was used to assess the susceptibility to colistin. Mating experiments were carried out to evaluate transferability of the mcr-genes. A total of 19 mcr-1 and one mcr-4 positive isolates were detected, 15 from pigs distributed during the study period, and five from storks collected in 2011. No other mcr-variants were found. The MICs for colistin ranged between 4 and >4 mg/L. High diversity of STs were detected among the mcr-1 positive E. coli isolates, with only ST-10 shared between pigs and white storks. Except for one isolate, all were genotypic and phenotypically MDR, and five of them also harbored cephalosporin resistance genes (bla CTX-M- 14, bla SHV- 12, and three bla CMY- 2). mcr-1 genes were mobilizable by conjugation, associated with IncX4, IncHI2, and IncI2 plasmids. In our study, mcr-1 genes have been circulating in pig farms since 2005 harbored by a variety of E. coli clones. Its persistence may be driven by co-selection since plasmids containing mcr-1 also exhibit resistance to multiple drugs used in veterinary medicine. Furthermore, this is the first report of the presence of mcr-1 gene in isolates from white storks in Spain. This finding highlights the potential importance of wildlife that forage at urban household waste landfills in the transmission and spread of colistin resistance genes.info:eu-repo/semantics/publishedVersio