60 research outputs found
"Bio-glues" to Enhance Slipperiness of Mucins: Improved Lubricity and Wear Resistance of Porcine Gastric Mucin (PGM) Layers Assisted by Mucoadhesion with Chitosan
A synergetic lubricating effect between porcine gastric mucin (PGM) and chitosan based on their mucoadhesive interaction is reported at a hydrophobic interface comprised of self-mated polydimethylsiloxane (PDMS) surfaces.</p
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Mesophase Separation and Probe Dynamics in Protein-Polyelectrolyte Coacervates
Protein–polyelectrolyte coacervates are self-assembling macroscopically monophasic biomacromolecular fluids whose unique properties arise from transient heterogeneities. The structures of coacervates formed at different conditions of pH and ionic strength from poly(dimethyldiallylammonium chloride) and bovine serum albumin (BSA), were probed using fluorescence recovery after photobleaching. Measurements of self-diffusion in coacervates were carried out using fluorescein-tagged BSA, and similarly tagged Ficoll, a non-interacting branched polysaccharide with the same size as BSA. The results are best explained by temporal and spatial heterogeneities, also inferred from static light scattering and cryo-TEM, which indicate heterogeneous scattering centers of several hundred nm. Taken together with previous dynamic light scattering and rheology studies, the results are consistent with the presence of extensive dilute domains in which are embedded partially interconnected 50–700 nm dense domains. At short length scales, protein mobility is unobstructed by these clusters. At intermediate length scales, proteins are slowed down due to tortuosity effects within the blind alleys of the dense domains, and to adsorption at dense/dilute domain interfaces. Finally, at long length scales, obstructed diffusion is alleviated by the break-up of dense domains. These findings are discussed in terms of previously suggested models for protein–polyelectrolyte coacervates. Possible explanations for the origin of mesophase separation are offered
Multiple Scale Reorganization of Electrostatic Complexes of PolyStyrene Sulfonate and Lysozyme
We report on a SANS investigation into the potential for these structural
reorganization of complexes composed of lysozyme and small PSS chains of
opposite charge if the physicochemical conditions of the solutions are changed
after their formation. Mixtures of solutions of lysozyme and PSS with high
matter content and with an introduced charge ratio [-]/[+]intro close to the
electrostatic stoichiometry, lead to suspensions that are macroscopically
stable. They are composed at local scale of dense globular primary complexes of
radius ~ 100 {\AA}; at a higher scale they are organized fractally with a
dimension 2.1. We first show that the dilution of the solution of complexes,
all other physicochemical parameters remaining constant, induces a macroscopic
destabilization of the solutions but does not modify the structure of the
complexes at submicronic scales. This suggests that the colloidal stability of
the complexes can be explained by the interlocking of the fractal aggregates in
a network at high concentration: dilution does not break the local aggregate
structure but it does destroy the network. We show, secondly, that the addition
of salt does not change the almost frozen inner structure of the cores of the
primary complexes, although it does encourage growth of the complexes; these
coalesce into larger complexes as salt has partially screened the electrostatic
repulsions between two primary complexes. These larger primary complexes remain
aggregated with a fractal dimension of 2.1. Thirdly, we show that the addition
of PSS chains up to [-]/[+]intro ~ 20, after the formation of the primary
complex with a [-]/[+]intro close to 1, only slightly changes the inner
structure of the primary complexes. Moreover, in contrast to the synthesis
achieved in the one-step mixing procedure where the proteins are unfolded for a
range of [-]/[+]intro, the native conformation of the proteins is preserved
inside the frozen core
Grafted block complex coacervate core micelles and their effect on protein adsorption on silica and polystyrene
We have studied the formation and the stability of grafted block complex coacervate core micelles (C3Ms) in solution and the influence of grafted block C3M coatings on the adsorption of the proteins β-lactoglobulin, bovine serum albumin, and lysozyme. The C3Ms consist of a grafted block copolymer PAA21-b-PAPEO14 (poly(acrylic acid)-b-poly(acrylate methoxy poly(ethylene oxide)), with a negatively charged PAA block and a neutral PAPEO block and a positively charged homopolymer P2MVPI (poly(N-methyl 2-vinyl pyridinium iodide). In solution, these C3Ms partly disintegrate at salt concentrations between 50 and 100 mM NaCl. Adsorption of C3Ms and proteins has been studied with fixed-angle optical reflectometry, at salt concentrations ranging from 1 to 100 mM NaCl. In comparison with the adsorption of PAA21-b-PAPEO14 alone adsorption of C3Ms significantly increases the amount of PAA21-b-PAPEO14 on the surface. This results in a higher surface density of PEO chains. The stability of the C3M coatings and their influence on protein adsorption are determined by the composition and the stability of the C3Ms in solution. A C3M-PAPEO14/P2MVPI43 coating strongly suppresses the adsorption of all proteins on silica and polystyrene. The reduction of protein adsorption is the highest at 100 mM NaCl (>90%). The adsorbed C3M-PAPEO14/P2MVPI43 layer is partly removed from the surface upon exposure to an excess of β-lactoglobulin solution, due to formation of soluble aggregates consisting of β-lactoglobulin and P2MVPI43. In contrast, C3M-PAPEO14/P2MVPI228 which has a fivefold longer cationic block enhances adsorption of the negatively charged proteins on both surfaces at salt concentrations above 1 mM NaCl. A single PAA21-b-PAPEO14 layer causes only a moderate reduction of protein adsorption
Enantiomeric and Diastereomeric Self-Assembled Multivalent Nanostructures : Understanding the Effects of Chirality on Binding to Polyanionic Heparin and DNA
A family of four self-assembling lipopeptides containing Ala-Lys peptides attached to a C16 aliphatic chain were synthesised. These compounds form two enantiomeric pairs that bear a diastereomeric relationship to one another (C16-l-Ala-l-Lys/C16-d-Ala-d-Lys) and (C16-d-Ala-l-Lys/C16-l-Ala-d-Lys). These diastereomeric pairs have very different critical micelle concentrations (CMCs). The self-assembled multivalent (SAMul) systems bind biological polyanions as a result of the cationic lysine groups on their surfaces. For heparin binding, there was no significant enantioselectivity, but there was a binding preference for the diastereomeric assemblies with lower CMCs. Conversely, for DNA binding, there was significant enantioselectivity for systems displaying d-lysine ligands, with a further slight preference for attachment to l-alanine, with the CMC being irrelevant
THE EFFECT OF HOFMEISTER IONS ON THERMODYNAMICS OF COMPLEX COACERVATION BETWEEN HYALURONIC ACID AND CHITOSAN
Franz Hofmeister\u27s research in 1888 led to the discovery of the Hofmeister series, an ion series the effects of which on the behavior of aqueous protein solutions were found to be significant. Other biomacromolecules also had their stability in solution determined by the presence of Hofmeister series. With respect to thermodynamics, this work seeks to understand the impact of this series on the complexation and coacervation of hyaluronic acid (HA) with chitosan (CHI) at three distinct pHs (3.25, 5.25, and 6.25) and two different molecular weights (HA, 1200 kDa & 199 kDa). While light microscopy images were used to confirm that the HA/CHI mixtures led to coacervates and not just precipitate particles, turbidimetric titration experiments were used to optimize the conditions affecting coacervation such as salt type, pH and concentration of buffering agent and polyelectrolyte. It has been determined that isothermal titration calorimetry is useful for comprehending the thermodynamics of coacervation. Our results indicate the validity of the direct Hofmeister effect for cations and the reverse Hofmeister effect for anions. Furthermore, the salt screening effect is readily evident since the interaction between the two polyelectrolytes is strongest when salt is absent. Additionally, it was found that as pH was increased, there was a stronger interaction between the two macromolecules
Trypsin Encapsulation within Pectin-Poly(diallyldimethylammonium Chloride) Complex Coacervates
Active substances like pharmaceuticals and enzymes can be shielded from a variety of unfavourable environmental conditions, including high pH, organic solvents, and chaotic agents, by employing complex coacervation as an encapsulation strategy. One of the goals of this research is to form complex coacervate droplets using pectin, a carbohydrate present in plant cell walls, and poly(diallyldimethylammonium chloride), a synthetic homopolymer. Electrostatic interactions between the positive charges in PDADMAC and the negative charges in pectin are the main factors for coacervation to assemble. Encapsulation of the trypsin enzyme within complex coacervates made of PDADMAC and pectin was examined as a function of mixing order of these polyelectrolytes and at various salt concentrations to learn more about protein encapsulation within complex coacervates. There were three different mixing orders used. We examined the effect of ionic strength on pectin-PDADMAC system by turbidimetric titrations at six different salt concentrations. Light microscopy allowed us to observe the formation of coacervate microdroplets at these salt concentrations. In order to determine the degree of ionization of pectin at the optimal pH for trypsin, which is 7.5, potentiometric titration studies were carried out on pectin. The most efficient mixing order is chosen for the subsequent experiments. The impact on enzyme encapsulation was also investigated by varying the amounts of trypsin and polyelectrolytes. Enzyme activity was assessed following the selection of the most efficient encapsulation technique. Circular dichroism studies were used to determine if the secondary structure of trypsin altered during encapsulation
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Protein–Polyelectrolyte Interactions
The interactions of proteins and polyelectrolytes lead to diverse applications in separations, delivery and wound repair, and are thus of interest to scientists in e.g. (a) glycobiology, (b) tissue engineering, (c) biosensing, and (d) pharmacology. This breadth is accompanied by an assortment of contexts and models in which polyelectrolytes are seen as (a) protein cognates assisting in complex cellular roles, (b) surrogates for the extracellular matrix, mimicking its hydration, mechanical and sequestering properties, (c) benign hosts that gently entrap, deposit and tether protein substrate specificity, and (d) selective but non-specific agents that modify protein solubility. Unsurprisingly, this literature is somewhat segregated by objectives and paradigms. We hope this review, which emphasizes publications over the last 8 years, represents and also counterbalances that divergence. An ongoing theme is the role of electrostatics, and we show how this leads to the variety of physical forms taken by protein–polyelectrolyte complexes. We present approaches towards analysis and characterization, motivated by the goal of structure–property elucidation. Such understanding should guide in applications, our third topic. We present recent developments in modeling and simulations of protein–polyelectrolyte systems. We close with a prospective on future developments in this field
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