Technology-Facilitated sexual violence perpetration and victimization among adolescents: a network analysis

Introduction The present study sought to analyze the relationships between diferent forms of technology-facilitated sexual violence (TFSV) perpetration and victimization, including gender- and sexuality-based harassment, digital sexual harassment, and image-based sexual abuse (IBSA), which includes both sextortion and non-consensual pornography. Methods The sample was composed of 1682 adolescents (865 girls; Mage=13.90, SDage=1.26) who completed self-report measures from November 2019 to March 2020. We conducted network analyses to analyze the relationships among diferent forms of TFSV perpetration and victimization. Results Overall, girls had higher victimization scores, whereas boys had higher scores in the perpetration of several forms of TFSV. Gender- and sexuality-based victimization formed a cluster with digital sexual harassment victimization, while sextortion and nonconsensual pornography victimization formed a diferentiated cluster. Forms of IBSA victimization and digital sexual harassment victimization were strongly associated with their perpetration counterparts for boys, but this was not the case for girls. Conclusions The results revealed that the diferent forms of victimization and perpetration appear to be related and suggest that TFSV is a gendered form of abuse. Policy Implications Findings from the present study suggest that preventive programs in schools and communities should be holistic and address various forms of TFSVOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Funding for this study was provided by the Ministerio de Ciencia e Innovación (the Spanish Government) Grant RTI2018‐101167‐B‐I0

Procesos tecnológicos y métodos de control en la hidrólisis de proteínas

Los hidrolizados de proteínas se utilizan ampliamente en tecnología alimentaria por sus propiedades nutricionaleso funcionales (solubilidad, poder emulsificante, capacidad espumante). En este trabajo se describen las técnicasempleadas para la obtención de estos hidrolizados y se comparan los diferentes métodos usados para el control deestos preparados: determinación del grado de hidrólisis, tamaño de los péptidos, distribución de pesos molecularesy contenido en aminoácidos y péptidos

Stability of the online grooming victimization of minors: prevalence and association with shame, guilt, and mental health outcomes over one year

Introduction: Online grooming is the process by which an adult manipulates a minor by using information and communication technologies to interact sexually with that minor. The objective of this study was to analyze the stability of online grooming victimization among minors and its relationship with demographic variables (e.g., gender, age, and sexual orientation), emotions of shame and guilt, and depression and anxiety symptoms. Methods: The participants were 746 adolescents aged 12–14 years when the study started (Mage = 13.34, SD = 0.87) who completed self‐reports at Time 1 (T1) and 1 year later at Time 2 (T2). Among them, 400 were girls, 344 were boys, and two were nonbinary. At each time point, the minors self‐reported their online grooming experiences during the previous year. Results: The results showed that 11.8% (n = 89) of the participants were T1‐victims, 13% (n = 95) were T2‐victims, and 11% (n = 81) were stable victims of online grooming. Stability in victimization was related to being older, being a sexual minority, being born abroad, having separated or divorced parents, and having parents with a lower education level. Stable victims showed higher shame and guilt scores at T1 than did T1‐victims, indicating that elevated levels of shame and guilt could contribute to the persistence of online grooming over time. Conclusions: Overall, adolescents in the stable victim category presented more depression and anxiety symptoms. Intervention strategies should address emotions related to victimization, such as shame and guilt, as well as depression and anxiety symptomsMinisterio de Ciencia e Innovación (Spanish Government), Grant/Award Number: RTI2018‐ 101167‐B‐I0

Cardiac electrical defects in progeroid mice and Hutchinson-Gilford progeria syndrome patients with nuclear lamina alterations

Hutchinson–Gilford progeria syndrome (HGPS) is a rare genetic disease caused by defective prelamin A processing, leading to nuclear lamina alterations, severe cardiovascular pathology, and premature death. Prelamin A alterations also occur in physiological aging. It remains unknown how defective prelamin A processing affects the cardiac rhythm. We show age-dependent cardiac repolarization abnormalities in HGPS patients that are also present in the Zmpste24-/- mouse model of HGPS. Challenge of Zmpste24-/- mice with the ß-adrenergic agonist isoproterenol did not trigger ventricular arrhythmia but caused bradycardia-related premature ventricular complexes and slow-rate polymorphic ventricular rhythms during recovery. Patch-clamping in Zmpste24-/- cardiomyocytes revealed prolonged calcium-transient duration and reduced sarcoplasmic reticulum calcium loading and release, consistent with the absence of isoproterenol-induced ventricular arrhythmia. Zmpste24-/- progeroid mice also developed severe fibrosis-unrelated bradycardia and PQ interval and QRS complex prolongation. These conduction defects were accompanied by overt mislocalization of the gap junction protein connexin43 (Cx43). Remarkably, Cx43 mislocalization was also evident in autopsied left ventricle tissue from HGPS patients, suggesting intercellular connectivity alterations at late stages of the disease. The similarities between HGPS patients and progeroid mice reported here strongly suggest that defective cardiac repolarization and cardiomyocyte connectivity are important abnormalities in the HGPS pathogenesis that increase the risk of arrhythmia and premature death.Peer ReviewedPostprint (published version

Pt(II)-dendrimers as bio-imaging marker for bacteria in two-photon excitation microscopy

The use of luminescent markers based on metal complexes in two-photon excitation microscopy techniques are of great interest in the field of bioimaging. However, despite the excellent luminescent properties of Pt(II) complexes, their application in this field is still limited, due to their poor solubility and quenching problems in aqueous media [1]. The insertion of a Pt(II) complex into a dendritic structure, gives as a result an unique luminescent marker soluble in biological media. Dendrimers provides excellent properties to the metal complex such as solubility in aqueous media, protection against quenching processes and binding to bacterial surfaces. The new probe can be used as bacteria cells marker in luminescent microscopy, operating under one or two-photon excitation (OPE/TPE) conditions, as well as in electron microscopy, thus providing a powerful tool in the field of bioimaging.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Tailoring two-photon fluorescent probes for pH bioimaging in living cells

Fluorescence biosensors are indispensable basic tools in modern biology. These type of molecules allow real-time visualization of biological events inside living cells. Especially important in many of these processes (proliferation, apoptosis or defense tasks) is the control of the cellular pH. In consequence, a great variety of structural models have been developed for pH bioimaging in fluorescence microscopy. Nonetheless, these efforts have been mainly focused on the development of one-photon (OP) probes. Recently, we described a biosensor with excellent photophysical properties and appropriate two-photon absorption (TPA) behavior. This sensor allows selective and specific detection of hydroxyl radicals solely inside lysosomes.Based on this scaffold, we have synthesized and characterized new TPA fluorescent probes. These molecules have an “off-on” response to different pH environments with a strong selectivity and sensitivity toward H+. These naphthalene-indolenine derivatives have a high synthetic versatility through affordable and efficient synthesis. The synthetic modification of this model allows tuning subcellular targets through minor modifications and without affecting their emission properties. The effectiveness of these probes and their structural modifications for different pH-related applications has been probed in mouse embrionary fibroblast (MEF) cells.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Optimización del problema de reposición de tiendas usando algoritmo conjunto de PSO y Recocido Simulado

El objetivo de esta trabajo es optimizar la reposición en tiendas garantizando una altadisponibilidad y rotación del producto, minimizando con ello los costes, especialmente,los derivados por exceso de almacenamiento o, por el contrario, los costes de rotura destock para un modelo multi-producto, multi-ubicación y multi-periodo. Para este fin, seha implementado una versión que conjuga dos meta-heurísticas distintas: ParticleSwarm Optimization (PSO) y Recocido Simulado (Simulated Annealing)

Origin of congenital coronary arterio-ventricular fistulae from anomalous epicardial and myocardial development.

Coronary Artery Fistulae (CAFs) are cardiac congenital anomalies consisting of an abnormal communication of a coronary artery with either a cardiac chamber or another cardiac vessel. In humans, these congenital anomalies can lead to complications such as myocardial hypertrophy, endocarditis, heart dilatation, and failure. Unfortunately, despite their clinical relevance, the aetiology of CAFs remains unknown. In this work, we have used two different species (mouse and avian embryos) to experimentally model CAFs morphogenesis. Both conditional Itga4 (alpha 4 integrin) epicardial deletion in mice and cryocauterisation of chick embryonic hearts disrupted epicardial development and ventricular wall growth, two essential events in coronary embryogenesis. Our results suggest that myocardial discontinuities in the embryonic ventricular wall promote the early contact of the endocardium with epicardial-derived coronary progenitors at the cardiac surface, leading to ventricular endocardial extrusion, precocious differentiation of coronary smooth muscle cells, and the formation of pouch-like aberrant coronary-like structures in direct connection with the ventricular lumen. The structure of these CAF-like anomalies was compared with histopathological data from a human CAF. Our results provide relevant information for the early diagnosis of these congenital anomalies and the molecular mechanisms that regulate their embryogenesis.The authors thank Dr. A. Rojas (CABIMER, Sevilla, Spain) and Prof. Thalia Papayannopoulou (University of Washington, WA, USA) for sharing with us the G2- Gata4-Cre and Itga4-floxed mouse lines, respectively. We also thank Vanessa Benhamo (Institut Imagine) for her expert support with HREM. Finally, we thank all members of “DeCA” laboratory (University of Málaga, Málaga, Spain), and the “Heart Morphogenesis” laboratory (Institut Imagine and Institut Pasteur, Paris, France) for their help and fruitful discussions on this paper. This work was supported by the Spanish Ministry of Science, R+D+i National Programme [grants RTI2018-095410-RBI00 and PID2021-122626-OB-I00], Spanish Ministry of Science-ISCIII [grant number RD16/0011/0030], and University of Málaga [grant number UMA18-FEDERJA-146] to [JMPP]; Consejería de Salud y Familias, Junta de Andalucía [grant number PIER-0084- 2019] to [JAGD]; University of Málaga [grant number I Plan Propio-UMA-A.4] to [ARV]; Spanish Ministry of Science, Innovation, and Universities (MCIU) (CIBER CV) [grant numbers PID2019-104776RB-I00 and CB16/11/00399] to [JLDLP].S