145 research outputs found
Structure of the ATP-Synthase from Chloroplasts and Mitochondria Studied by Electron Microscopy
The structure of the ATP-synthase, F0F1 , from spinach chloroplasts and beef heart mitochondria has been investigated by electron microscopy with negatively stained specimens. The detergent-solubilized ATP-synthase forms string-like structures in which the F0 parts are aggregated. In most cases, the F, parts are arranged at alternating sides along the string. The F0 part has an approximate cylindrical shape with heights of 8.3 and 8.9 nm and diameters of 6.2 and 6.4 nm for the chloroplast and mitochondrial enzyme, respectively. The F, parts are disk-like structures with a diameter of about 11.5 nm and a height of about 8.5 nm. The F, parts are attached to the strings, composed of Fn parts, in most cases, with their smallest dimension parallel to the strings. The stalk connecting F0 and F, has a length of 3.7 nm and 4.3 nm and a diameter of 2.7 nm and 4.3 nm for the chloroplast and mitochondrial enzyme, respectively
Molecular states in carbon nanotube double quantum dots
We report electrical transport measurements through a semiconducting
single-walled carbon nanotube (SWNT) with three additional top-gates. At low
temperatures the system acts as a double quantum dot with large inter-dot
tunnel coupling allowing for the observation of tunnel-coupled molecular states
extending over the whole double-dot system. We precisely extract the tunnel
coupling and identify the molecular states by the sequential-tunneling line
shape of the resonances in differential conductance.Comment: 5 pages, 4 figure
Defining and controlling double quantum dots in single-walled carbon nanotubes
We report the experimental realization of double quantum dots in
single-walled carbon nanotubes. The device consists of a nanotube with source
and drain contact, and three additional top-gate electrodes in between. We show
that, by energizing these top-gates, it is possible to locally gate a nanotube,
to create a barrier, or to tune the chemical potential of a part of the
nanotube. At low temperatures we find (for three different devices) that in
certain ranges of top-gate voltages our device acts as a double quantum dot,
evidenced by the typical honeycomb charge stability pattern.Comment: 9 pages, 3 figure
A major electronics upgrade for the H.E.S.S. Cherenkov telescopes 1-4
The High Energy Stereoscopic System (H.E.S.S.) is an array of imaging
atmospheric Cherenkov telescopes (IACTs) located in the Khomas Highland in
Namibia. It consists of four 12-m telescopes (CT1-4), which started operations
in 2003, and a 28-m diameter one (CT5), which was brought online in 2012. It is
the only IACT system featuring telescopes of different sizes, which provides
sensitivity for gamma rays across a very wide energy range, from ~30 GeV up to
~100 TeV. Since the camera electronics of CT1-4 are much older than the one of
CT5, an upgrade is being carried out; first deployment was in 2015, full
operation is planned for 2016. The goals of this upgrade are threefold:
reducing the dead time of the cameras, improving the overall performance of the
array and reducing the system failure rate related to aging. Upon completion,
the upgrade will assure the continuous operation of H.E.S.S. at its full
sensitivity until and possibly beyond the advent of CTA. In the design of the
new components, several CTA concepts and technologies were used and are thus
being evaluated in the field: The upgraded read-out electronics is based on the
NECTAR readout chips; the new camera front- and back-end control subsystems are
based on an FPGA and an embedded ARM computer; the communication between
subsystems is based on standard Ethernet technologies. These hardware solutions
offer good performance, robustness and flexibility. The design of the new
cameras is reported here.Comment: Proceedings of the 34th International Cosmic Ray Conference, 30 July-
6 August, 2015, The Hague, The Netherland
An optimized protocol for assessment of sputum macrorheology in health and muco-obstructive lung disease
Background: Airway mucus provides important protective functions in health and abnormal viscoelasticity is a hallmark of muco-obstructive lung diseases such as cystic fibrosis (CF). However, previous studies of sputum macrorheology from healthy individuals and patients with CF using different experimental protocols yielded in part discrepant results and data on a systematic assessment across measurement settings and conditions remain limited.
Objectives: The aim of this study was to develop an optimized and reliable protocol for standardized macrorheological measurements of airway mucus model systems and native human sputum from healthy individuals and patients with muco-obstructive lung disease.
Methods: Oscillatory rheological shear measurements were performed using bovine submaxillary mucin (BSM) at different concentrations (2% and 10% solids) and sputum samples from healthy controls (n = 10) and patients with CF (n = 10). Viscoelastic properties were determined by amplitude and frequency sweeps at 25°C and 37°C with or without solvent trap using a cone-plate geometry.
Results: Under saturated atmosphere, we did not observe any temperature-dependent differences in 2% and 10% BSM macrorheology, whereas in the absence of evaporation control 10% BSM demonstrated a significantly higher viscoelasticity at 37°C. Similarly, during the measurements without evaporation control at 37°C we observed a substantial increase in the storage modulus GâČ and the loss modulus Gâł of the highly viscoelastic CF sputum but not in the healthy sputum.
Conclusion: Our data show systematically higher viscoelasticity of CF compared to healthy sputum at 25°C and 37°C. For measurements at the higher temperature using a solvent trap to prevent evaporation is essential for macrorheological analysis of mucus model systems and native human sputum. Another interesting finding is that the viscoelastic properties are not much sensitive to the applied experimental deformation and yield robust results despite their delicate consistency. The optimized protocol resulting from this work will facilitate standardized quantitative assessment of abnormalities in viscoelastic properties of airway mucus and response to muco-active therapies in patients with CF and other muco-obstructive lung diseases
Electric Field Control of Spin Transport
Spintronics is an approach to electronics in which the spin of the electrons
is exploited to control the electric resistance R of devices. One basic
building block is the spin-valve, which is formed if two ferromagnetic
electrodes are separated by a thin tunneling barrier. In such devices, R
depends on the orientation of the magnetisation of the electrodes. It is
usually larger in the antiparallel than in the parallel configuration. The
relative difference of R, the so-called magneto-resistance (MR), is then
positive. Common devices, such as the giant magneto-resistance sensor used in
reading heads of hard disks, are based on this phenomenon. The MR may become
anomalous (negative), if the transmission probability of electrons through the
device is spin or energy dependent. This offers a route to the realisation of
gate-tunable MR devices, because transmission probabilities can readily be
tuned in many devices with an electrical gate signal. Such devices have,
however, been elusive so far. We report here on a pronounced gate-field
controlled MR in devices made from carbon nanotubes with ferromagnetic
contacts. Both the amplitude and the sign of the MR are tunable with the gate
voltage in a predictable manner. We emphasise that this spin-field effect is
not restricted to carbon nanotubes but constitutes a generic effect which can
in principle be exploited in all resonant tunneling devices.Comment: 22 pages, 5 figure
Validity and worth in the science curriculum: learning school science outside the laboratory
It is widely acknowledged that there are problems with school science in many developed countries of the world. Such problems manifest themselves in a progressive decline in pupil enthusiasm for school science across the secondary age range and the fact that fewer students are choosing to study the physical sciences at higher levels and as careers. Responses to these developments have included proposals to reform the curriculum, pedagogy and the nature of pupil discussion in science lessons. We support such changes but argue from a consideration of the aims of science education that secondary school science is too rooted in the science laboratory; substantially greater use needs to be made of out-of-school sites for the teaching of science. Such usage should result in a school science education that is more valid and more motivating and is better at fulfilling defensible aims of school science education. Our contention is that laboratory-based school science teaching needs to be complemented by out-of-school science learning that draws on the actual world (e.g. through fieldtrips), the presented world (e.g. in science centres, botanic gardens, zoos and science museums) and the virtual worlds that are increasingly available through information and communications technologies (ICT)
Serum neurofilament dynamics predicts neurodegeneration and clinical progression in presymptomatic Alzheimer's disease
Neurofilament light chain (NfL) is a promising fluid biomarker of disease progression for various cerebral proteopathies. Here we leverage the unique characteristics of the Dominantly Inherited Alzheimer Network and ultrasensitive immunoassay technology to demonstrate that NfL levels in the cerebrospinal fluid (nâ=â187) and serum (nâ=â405) are correlated with one another and are elevated at the presymptomatic stages of familial Alzheimer's disease. Longitudinal, within-person analysis of serum NfL dynamics (nâ=â196) confirmed this elevation and further revealed that the rate of change of serum NfL could discriminate mutation carriers from non-mutation carriers almost a decade earlier than cross-sectional absolute NfL levels (that is, 16.2 versus 6.8 years before the estimated symptom onset). Serum NfL rate of change peaked in participants converting from the presymptomatic to the symptomatic stage and was associated with cortical thinning assessed by magnetic resonance imaging, but less so with amyloid-ÎČ deposition or glucose metabolism (assessed by positron emission tomography). Serum NfL was predictive for both the rate of cortical thinning and cognitive changes assessed by the Mini-Mental State Examination and Logical Memory test. Thus, NfL dynamics in serum predict disease progression and brain neurodegeneration at the early presymptomatic stages of familial Alzheimer's disease, which supports its potential utility as a clinically useful biomarker
Decreased body mass index in the preclinical stage of autosomal dominant Alzheimer\u27s disease
The relationship between body-mass index (BMI) and AlzheimeĆs disease (AD) has been extensively investigated. However, BMI alterations in preclinical individuals with autosomal dominant AD (ADAD) have not yet been investigated. We analyzed cross-sectional data from 230 asymptomatic members of families with ADAD participating in the Dominantly Inherited Alzheimer Network (DIAN) study including 120 preclinical mutation carriers (MCs) and 110 asymptomatic non-carriers (NCs). Differences in BMI and their relation with cerebral amyloid load and episodic memory as a function of estimated years to symptom onset (EYO) were analyzed. Preclinical MCs showed significantly lower BMIs compared to NCs, starting 11.2 years before expected symptom onset. However, the BMI curves begun to diverge already at 17.8 years before expected symptom onset. Lower BMI in preclinical MCs was significantly associated with less years before estimated symptom onset, higher global AÎČ brain burden, and with lower delayed total recall scores in the logical memory test. The study provides cross-sectional evidence that weight loss starts one to two decades before expected symptom onset of ADAD. Our findings point toward a link between the pathophysiology of ADAD and disturbance of weight control mechanisms. Longitudinal follow-up studies are warranted to investigate BMI changes over time
Hamiltonian approach to the charge transfer statistics of Kondo quantum dots contacted by a normal metal and a superconductor
We analyze the full counting statistics (FCS) of quantum dots in the Kondo
regime contacted by normal and superconducting leads or an STM tip. To describe
the Kondo resonance we use an effective model for the quantum dot in the Kondo
regime in combination with the FCS for tunneling contacts calculated using the
Hamiltonian approach. We show that the situation of weak coupling to the
superconducting electrode in the case of superconductor gap being larger than
the Kondo resonance width can be easily handled and verify the method by
comparing our theoretical predictions to experimental data. This allows us to
make predictions for the noise and cross-correlation in setups involving a
superconductor. We find that a positive cross-correlation is possible in the
case of a superconductor and two normal leads contacted via two quantum dots in
the Kondo regime.Comment: 7 pages, 4 figure
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