The helium cryogenic plant for the CMS superconducting magnet

A new helium refrigeration plant with a cooling capacity of 800 W at 4.45 K, 4500 W between 60 K and 80 K, and 4 g/s liquefaction simultaneously has been designed and is presently being constructed by Air Liquide for CERN. The refrigeration plant will provide the cooling power for the cool down and the operation of the CMS (Compact Muon Solenoid) superconducting coil whose cold mass weighs 225 t. The refrigeration plant will at first be installed in a surface building for the tests of the superconducting magnet. On completion of the tests the cold box will be moved to its final underground position next to the CMS experimental cavern. This paper presents the process design, describes the main components and explains their selection. (4 refs)

Whole body cryotherapy and recovery from exercise induced muscle damage: A systematic review

Introduction Cold therapies are used regularly in medicine for their analgesic and anti-inflammatory effects. Whole-body cryotherapy (WBC) involves exposure to air maintained between -110 and -160oC, and is hypothesised to reduce pain, local and systemic inflammation. WBC has recently become popular in an exercise and sporting context as a recovery method after skeletal muscle damage, however, research examining the efficacy of WBC in an athletic context is minimal. This review seeks to summarise the evidence for the effects of WBC on exercise recovery measures. Methods Electronic database searches were conducted from March to April 2013. Six large online databases were used; MEDLINE, SPORTDiscus, Scopus, Web of Science, PubMed and AMED. The search targeted human studies with an exercise task, and WBC intervention. Results included randomised controlled trials (RCT’s), uncontrolled trials and crossover designs. Results A total of 8 studies were included in the review. Two RCT’s, four crossover trials and two uncontrolled trials were identified. Five studies showed WBC had no effect on markers of muscle damage or inflammation post exercise, while 3 studies show a positive effect. Three out of the eight studies measured maximal muscle force production and subjective pain levels following exercise and WBC, with two showing WBC had a positive effect on muscle force recovery and pain. A meta-analysis was not conducted due to the heterogeneity of the studies. Conclusion The current evidence for the efficacy of WBC on exercise recovery is unclear. Published studies report mixed findings, and the study designs make these results difficult to interpret. As WBC is proposed as an aid to recovery in an athletic population, repeated measures of performance, muscle force production and pain are of importance to the athlete, however, are minimally reported in the literature. Cold water immersion (CWI) is widely used in an athletic setting for recovery, and has much literature supporting its use for the reduction of pain post-exercise. Well-designed RCT’s with controlled exercise interventions targeting performance measures are needed, in particular comparison of WBC with CWI data is needed for evaluation

Lymphocyte and Monocyte Hsp72 Responses to Exercise in Athletes with Prior Exertional Heat Illness

ABSTRACT Introduction. Exertional heatstroke is a serious disorder that can be fatal especially if treatment is delayed. Heat shock protein 72 (Hsp72) is strongly induced by heat, and can be protective against a subsequent stress that may be the same or of a different form. In animal models it has been shown that upregulation of Hsp72 is protective against heatstroke. There is a natural variability in the amount and/or inducibility of Hsp72 in cells and tissues between individuals, and it is possible that impaired expression levels could make some athletes more prone to heat illness. The purpose of this study was to examine Hsp72 expression in lymphocytes and monocytes of young (\u3c40 years) athletes who had previously experienced, but recovered from serious heatstroke during exercise in the heat. Methods. Fourteen athletes ran on a treadmill for 60 min at 72% maximal oxygen uptake (o2max) in warm conditions (30°C, 40% relative humidity). One group consisted of athletes who had a previous history of exertional heat illness (EHI), while the control group (CON) had no previous history of EHI. Both groups were of similar age (29.7 ± 1.2 and 29.1 ± 2 years, CON vs EHI) and fitness (o2max 65.7 ± 2 and 64.5 ± 3 ml.kg-1.min-1, CON vs EHI). Rectal temperature was measured using a thermistor inserted to a depth of 10 cm past the anal sphincter. Hsp72 levels were measured in both monocytes and lymphocytes by flow cytometry before and immediately after the 60-min run, then after 60 min of recovery at an ambient temperature of 24°C. Results. Rectal temperature increased during the exercise period but there was no difference between groups, demonstrating that the EHI group had recovered from their heat illness and were not heat intolerant. Lymphocyte Hsp72 was lower in the EHI group after 60 min of exercise (p\u3c0.05), while monocyte Hsp72 was not different between groups. Conclusion. Our study found a lower lymphocyte Hsp72 concentration during exercise in athletes who had previously collapsed with serious EHI. Further research is needed to determine whether lower lymphocyte Hsp72 is a factor that may predispose athletes to develop EHI

Effects of whole body cryotherapy and cold water immersion on immune and inflammatory markers following exercise induced muscle damage

Introduction: Cold therapies are used regularly in medicine for their analgesic and anti-inflammatory effects. Whole-body cryotherapy (WBC) involves exposure to air maintained between -110 and -160oC, and is hypothesised to reduce pain, local and systemic inflammation. WBC has recently become popular in an exercise and sporting context as a recovery method after skeletal muscle damage. However, research examining the efficacy of WBC in an athletic context is minimal, in particular, studies comparing WBC to currently accepted recovery methods are lacking. Cold water immersion (CWI) is a widely researched and applied method of skeletal muscle recovery in sport science. As yet, no study has compared the proposed new method of WBC and the currently practiced method of CWI. We have designed a randomised control trial to examine the efficacy of WBC, as compared with CWI on recovery from a bout of eccentric muscle damage. Methods: Sixty healthy male subjects will perform skeletal muscle function tests and an eccentric muscle damage protocol of their left quadriceps femoris, using an isokinetic dynamometer. They will then be randomly assigned to one of 3 groups, WBC, CWI or a passive recovery control (PAS). The WBC will expose subjects to -160°C for 3min, using cold air. The CWI condition involves whole body exposure for 3min, in water maintained at 12°C. The PAS will have subjects seated comfortably at room temperature following the exercise protocol. Blood samples, muscle functional measurements and pain reports will be taken before muscle damage, immediately following damage, prior to therapy administration and post therapy. Further follow up measures to be taken 6 h post, 24 h and 7 days post. Blood samples will be analysed for changes in interleukins 6, 8 and 10, creatine kinase and leukocyte population kinetics. Results: Testing is being conducted. Results to be presented at the international society of exercise immunology (ISEI) symposium in September 2013

ISOGAL-DENIS detection of red giants with weak mass loss in the Galactic Bulge

The ISOGAL project is a survey of the stellar populations, structure, and recent star formation history of the inner disk and bulge of the Galaxy. ISOGAL combines 15 and 7micron ISOCAM observations with DENIS IJKs data to determine the nature of a source and the interstellar extinction. In this paper we report an ISOGAL study of a small field in the inner Galactic Bulge (l=0deg, b=1.0deg, area=0.035 sq. deg) as a prototype of the larger area ISOGAL survey of the inner Galaxy. The five wavelengths of ISOGAL+DENIS, together with the relatively low and constant extinction in front of this specific field, allow reliable determination of the nature of the sources. The primary scientific result of this paper is evidence that the most numerous class of ISOGAL 15micron sources are Red Giants in the Galactic bulge and central disk, with luminosities just above or close to the RGB tip and weak mass-loss rates. They form loose sequences in the magnitude-colour diagrams [15]/Ks-[15] and [15]/[7]-[15]. Their large excesses at 15micron with respect to 2micron and 7micron is due to circumstellar dust produced by mass-loss at low rates. These ISOGAL results are the first systematic evidence and study of dust emission at this early stage (''Intermediate'' AGB), before the onset of the large mass-loss phase. It is thus well established that efficient dust formation is already associated with such low mass-loss rates during this early phase.Comment: 15 pages, 9 figures, accepted for publication in Astronomy and Astrophysics Journa

Contourite distribution and bottom currents in the NW Mediterranean Sea: Coupling seafloor geomorphology and hydrodynamic modelling

Contourites are common morphological features along continental margins where currents encounter the seafloor. They can provide long-term archives of palaeoceanography, may be prone to sediment instability, and can have a great potential for hydrocarbon exploration. Despite their importance and increasingly recognised ubiquitous occurrence worldwide, the link between oceanographic processes and contourite features is poorly constrained. In particular, it is unclear under which specific conditions sediments are mobilised, modified and deposited by bottom currents. Here, we aim to determine key bottom current characteristics (velocity and bottom shear stress) affecting contourite deposition, by assuming that recent oceanographic regimes may be extended back in time over the past glacial-interglacial cycles, with strong winter circulation assumed similar to glacial conditions and weak summer circulation to interglacials. We present an integrated study from the NW Mediterranean Sea that couples results of the MARS3D hydrodynamic model with high-resolution sedimentological and geophysical data (piston cores, multibeam bathymetry and high resolution seismic data). Near bottom circulation was modelled during winter and summer 2013 as representative of past periods of high and low current intensity, respectively. Model results match well with the extent of contourite depositional systems and their different localised morphologic elements. We deduce that higher intensity events control the formation of erosional features such as moats and abraded surfaces. The heterogeneous distribution of bottom-current intensity on slopes explains the development of different types of contourite drifts. Plastered drifts form in zones of low bottom-current velocities constrained upslope and downslope by higher current velocities. Separated elongated mounded drifts develop where fast bottom-currents decelerate at foot of the slope. In contrast, no mounded contourite morphologies develop when the current velocity is homogeneous across the slope, especially in margins prone to downslope sediment transport processes. In confined basins, gyres may transport sediment in suspension from a margin with a high sediment supply to an adjacent starved margin, favouring the development of fine-grained contourites in the latter. Our results provide new insights into how detailed bottom-circulation modelling and seafloor geomorphological analyses can improve the understanding of palaeoflow-regimes, at least over time spans when the overall paleogeography and the distribution of contourite drifts is comparable to present-day conditions. The approach of coupled hydrodynamic models and geomorphological interpretations proposed here for depositional, erosional and mixed contourite features may be used to understand other areas affected by bottom currents, and for a better conceptual understanding of bottom-current processes and their interactions with the seafloor

The toxicity and intraspecific variability of Alexandrium andersonii Balech

The toxicity of Alexandrium andersonii Balech is unclear and its intraspecific variability has yet to be studied. To address these gaps in our knowledge, in the present work five strains of A. andersonii from four different localities were characterized. The results showed that despite genetic homogeneity in the 5.8-ITS (internal transcribed spacer) and large subunit (LSU) regions and similar growth rates, strains originating from different locations varied with respect to cell size, the ratios of certain pigments, and their growth patterns. Cultures of the strains grown at 20 8C were analyzed for toxicity using four different methodologies. The two officially established methods, mouse bioassay and high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and post-column reaction analysis of PSP toxins, failed to show the toxicity of any strain. Strains grown at 14 8C were also negative for PSP toxins by HPLC-FLD. However, strains grown at 20 8C exhibited both a response characteristic of the presence of toxin-inhibiting voltage-gated sodium channels, as demonstrated in a neuroblastoma neuro- 2a cell-based assay, as well as hemolytic activity in a sheep red blood cell assay

Chronic erythropoietin treatment improves diet-induced glucose intolerance in rats

Erythropoietin (EPO) ameliorates glucose metabolism through mechanisms not fully understood. In this study, we investigated the effect of EPO on glucose metabolism and insulin signaling in skeletal muscle. A 2-week EPO treatment of rats fed with a high-fat diet (HFD) improved fasting glucose levels and glucose tolerance, without altering total body weight or retroperitoneal fat mass. Concomitantly, EPO partially rescued insulin-stimulated AKT activation, reduced markers of oxidative stress, and restored heat-shock protein 72 expression in soleus muscles from HFD-fed rats. Incubation of skeletal muscle cell cultures with EPO failed to induce AKT phosphorylation and had no effect on glucose uptake or glycogen synthesis. We found that the EPO receptor gene was expressed in myotubes, but was undetectable in soleus. Together, our results indicate that EPO treatment improves glucose tolerance but does not directly activate the phosphorylation of AKT in muscle cells. We propose that the reduced systemic inflammation or oxidative stress that we observed after treatment with EPO could contribute to the improvement of whole-body glucose metabolism.Corinne Caillaud, Mie Mechta, Heidi Ainge, Andreas N Madsen, Patricia Ruell, Emilie Mas, Catherine Bisbal, Jacques Mercier, Stephen Twigg, Trevor A Mori, David Simar and Romain Barrè

Pacti: Scaling Assume-Guarantee Reasoning for System Analysis and Design

Contract-based design is a method to facilitate modular system design. While there has been substantial progress on the theory of contracts, there has been less progress on scalable algorithms for the algebraic operations in this theory. In this paper, we present: 1) principles to implement a contract-based design tool at scale and 2) Pacti, a tool that can efficiently compute these operations. We then illustrate the use of Pacti in a variety of case studies