An Organizational Culture Assessment Using the Competing Values Framework: A Profile of Ohio State University Extension

The competing values framework was used to describe the organizational culture type exhibited by Ohio State University Extension (OSU Extension) personnel. This framework assesses the dominant organizational culture based on four culture types: Clan, Hierarchy, Adhocracy, and Market. OSU Extension personnel exhibited a Clan culture type as dominant in both the current and preferred situations. The Clan culture portrays OSU Extension as an organization that concentrates on internal maintenance with flexibility, concern for people, and sensitivity for customers. The study suggests implications for Extension nationwide

Modification, Ablation and Hardening of Metallic Surfaces by a Cryogenic Nitrogen Jet

AbstractThis contribution gives the first results of an ongoing research aiming at developing a new surface treatment technique usinga supercritical nitrogen jet, named Jazolthop, for surface modification. As nitrogen is naturally recycled within air, this new process has a high potential for surface treatment without any chemical, physical or sewage effluents. This contribution shows that, pending on the operating condition, the technique can be used (i) under a stripping or ablation mode as well as, in a “less conventional” approach, (ii) for surface hardening.Illustration of the ablation mode is given for a Ti-6Al-4V alloy treated under static conditions using an intrusive jet. After 2min of treatment, a thickness of 200μm was removed from the surface by successive stripping out of micro-chips.Illustration of the hardening mode is given through the analysis of stainless steels treated under the cryogenic jet at a moving torch velocity of 5mm/min. The jet conditions were selected to be less intrusive and trigger the martensitic transformation without creating surface flaws of micro-cavities. In this case, the hardness of the steels was more than doubled after the passage of the cryogenic jet

El taller como mediaci?n para el desarrollo de la competencia comunicativa oral en los estudiantes del grado 5? de la instituci?n educativa Jos? Mar?a Carbonell sede Rafael Rocha del municipio de San antonio

108 p. Recurso Electr?nicoEn este trabajo se presenta el dise?o de una estrategia pedag?gica titulada ?El taller teatral como mediaci?n para el desarrollo de la competencia comunicativa oral?, se refiere la experiencia pedag?gica de la autora a partir de la indagaci?n que hace sobre los procesos de comunicaci?n oral en los estudiantes del grado quinto de educaci?n b?sica de la Instituci?n Educativa Jos? Mar?a Carbonell, sede Rafael Rocha perteneciente al municipio de San Antonio, Tolima. Es as? como, esta propuesta est? basada en la identificaci?n, resignificaci?n y comprensi?n de los saberes espec?ficos del rol docente, que permitieron encontrar una diversidad de falencias en los procesos de producci?n oral de los dicentes. Ahora en segunda instancia, con la aplicaci?n de la estrategia pedag?gica que es un mediador que se constituye en un elemento pr?ctico para desarrollar en el ni?o habilidades que le permitan comprender las ideas y familiarizarse con la oralidad. De esta perspectiva, se fortalece la competencia comunicativa oral a trav?s del taller teatral en la elaboraci?n de textos teatrales e interpretaci?n de historias que desarrollan la imaginaci?n y la creatividad. Adem?s, facilita que el individuo sea capaz de suplir sus necesidades comunicativas utilizando todas sus potencialidades que lo hacen socializarse y, tomando todos los elementos que lo rodean como esenciales en el intercambio de experiencias. Por otra parte, esta estrategia tiene la intencionalidad de brindar un soporte te?rico que permita ubicar al lector en el conocimientos de ciertas ideas, criterios y conceptos que le ayudar?an a entender la situaci?n problem?tica que se aborda, al igual que otras razones necesarias por las cuales se est?n presentando, de tal manera, que en la transversalidad del referente te?rico se va encontrar la presencia de cada uno de sus postulados de los autores, tales como P?rez M (1998), Lomas (1997), Cassany, D (2005), Motos (2008), Blanco (2001), entre otros. Se concluye que, con este tipo de estrategia pedag?gica se puede estimular la participaci?n de los estudiantes por medio de actividades escolares que pueden permitirles crecer como individuos sensibles que fomenten sus aptitudes y destrezas en las artes dram?ticas debido a su relaci?n con el desarrollo de la competencia comunicativa oral, la cual provee al estudiante materiales ricos a la lengua y, adem?s, le permite aprender haciendo y disfrutando al mismo tiempo.On this work, it is presented the design of a pedagogical strategy titled Theatrical workshop as mediation tool for the development of oral communicative competence. It refers to the author's research experience based on the inquiry she makes about the oral communication processes in the students of fifth grade of basic education of the Educational Institution Jos? Mar?a Carbonell, Rafael Rocha headquarters belonging to the municipality of San Antonio, Tolima. It is like this, how this proposal is based on the identification, resignification and comprehension of specific knowledge belonging to the teacher which allowed to find out a diversity of lacks on the students? oral production processes. Now in the second instance, with the application of the pedagogical strategy which is a mediator that is constituted in a practical element to develop in the child abilities that allow him to understand the ideas and to become familiar with orality. From this perspective, this competition is strengthened in the theater workshop through the elaboration of theatrical texts and short stories, which develop imagination and creativity in order to equip students with resources to strengthen certain difficulties in the production of oral texts. In addition, it facilitates that the individual is able to meet their communicative needs using all his/her potentialities that make him/her socialize and taking all the elements that surround him/her as essential in the exchange of experiences. On the other hand, this strategy has the intention of providing a theoretical support that allows to place the reader in the knowledge of certain ideas, criteria and concepts that would help him/her to understand the problematic situation that is addressed, as well as other necessary reasons why It is being presented, in such a way, that in the transversality of the theoretical reference,the presence of each one of its postulates of the authors, such as P?rez M (1998), Lomas (1997), Cassany, D (2005), Nieto (1995), Motos (2008), Blanco (2001), among others, will be found. It is concluded that with this type of pedagogical strategy students' participation can be stimulated through school activities that may allow them to grow as sensitive individuals that promote their skills and abilities in the dramatic arts due to their relation to the use of the oral communicative competence, which provides the student with rich materials the authentic tongue and, in addition, allows him/her to learn while doing and enjoying at the same time. Keywords: pedagogical strategy, oral communicative competence, theater workshop, mediator

Extracellular Ca2+-sensing fluorescent protein biosensor based on a collagen-binding domain

The importance of extracellular gradients of biomolecules is increasingly appreciated in the processes of tissue development and regeneration, in health and disease. In particular, the dynamics of extracellular calcium concentration is rarely studied. Here, we present a low affinity Ca2+ biosensor based on Twitch-2B fluorescent protein fused with the cellulose- and collagen-binding peptides. These recombinant chimeric proteins can bind cellulose and collagen scaffolds and enable scaffold-based biosensing of Ca2+ in the proximity of cells in live 3D tissue models. We found that the Twitch-2B mutant is compatible with intensity-based ratiometric and fluorescence lifetime imaging microscopy (FLIM) measurement formats, under one- and two-photon excitation modes. Furthermore, the donor fluorescence lifetime of the biosensor displays response to [Ca2+] over a range of similar to 2-2.5 ns, making it attractive for multiplexed FLIM assays. To evaluate the performance of this biosensor in physiological measurements, we applied it to the live Lgr5-GFP mouse intestinal organoid culture and measured its responses to the changes in extracellular Ca2+ upon chelation with EGTA. When combined with spectrally resolved FLIM of lipid droplets using Nile red dye, we observed changes in cytoplasmic and basal membrane-associated lipid droplet composition in response to the extracellular Ca2+ depletion, suggesting that the intestinal epithelium can respond to and compensate such treatment. Altogether, our results demonstrate Twitch-2B as a prospective Ca2+ sensor for multiplexed FLIM analysis in a complex 3D tissue environment

Síntesis y evaluación citotoxica de derivados halogenados y peracetylados de nucleósidos en celulas de cancer de mama

Objectives. To make the synthesis of halogenated derivatives on the nitrogenous base and their respective acyl ester and amide type derivatives for all hydroxyl and amine groups of the uridine and cytarabine nucleosides, and evaluate cytotoxicity against breast cancer cell line. Methods. First, it was accomplished the halogenation reaction on the 5-position of the nitrogenous base, subsequently, the ester and amide derivatives were performed for all hydroxyl and amine group present in the nucleosides. Besides, the uridine acetonide derivatives as prepared by acid catalysis. The products were characterized by nuclear magnetic resonance spectroscopy (1H RMN y 13C RMN) and mass spectrometry in positive mode by direct injection. Derivatives were evaluated in Chinese hamster ovary (CHO-K1) and human breast cancer (MCF-7) cell lines. Results. The four derivatives were obtained with chlorine and bromine for the uridine and cytarabine, respectively, their respective per-acetylated derivatives, the per-acetylated nucleoside and the uridine acetonide; the compounds were obtained with efficiency over 90%. The per-acetylated nucleosides and the halogenated and per-acetylated derivatives did not show inhibitory effects on cell viability in MCF-7 cell line. However, the per-acetylated and halogenated derivatives presented a higher cytotoxic activity than their respective per-acetylated nucleoside. The uridine 3’,4’-acetonide showed a significant cytotoxicity on both cell lines. Conclusions. The per-acetylated nucleoside, and the respective halogenated derivatives with chlorine and bromine were obtained with high yields, nevertheless, these compounds did not exhibit a significant anti-proliferative activity (p˂0.05), possibly due to a low intra-cellular activation.Objetivos: Sintetizar derivados halogenados sobre la base nitrogenada, sus respectivos derivados tipo éster o amida de todos los grupos hidroxilo y amina presentes en los nucleósidos uridina y citarabina, y evaluar su actividad citotóxica sobre una línea celular de cáncer de mama. Metodología: primero se realizó la reacción de halogenación en la posición 5 de la base nitrogenada, posteriormente se formaron los ésteres y amidas de todos los grupos hidroxilos y amino presentes en los nucleósidos. Además, se preparó el derivado acetónido con catálisis ácida. Los compuestos se caracterizaron por espectroscopía de resonancia magnética nuclear (RMN 1H y RMN 13C) y espectrometría de masas por inyección directa en modo positivo. Los derivados se evaluaron sobre líneas celulares de tumor de Ovario de Hámster Chino (CHO) y de cáncer de mamá (MCF-7). Resultados: Se obtuvieron 4 derivados mono-halogenados con cloro y bromo de la uridina y citarabina, respectivamente, sus respectivos derivados per-acetilados, los nucleósidos per-acetilados y el acetónido de la uridina; los compuestos se obtuvieron con rendimientos superiores a 90%. Los nucleósidos per-acetilados, y los derivados per-acetilados y halogenados no exhibieron una inhibición significativa de la viabilidad celular en ambas líneas celulares, sin embargo, de estos, los derivados per-acetilados y halogenados presentaron mayor actividad citotóxica que los respectivos nucleósidos per-acetilados. El derivado acetónido de la uridina mostró citotoxicidad significativa sobre ambas líneas celulares. Conclusiones: se obtuvieron los nucleósidos per-acetilados y los respectivos derivados clorados y bromados de estos, con rendimientos altos, sin embargo, estos compuestos no exhibieron una actividad anti-proliferativa significativa (p˂0,05), posiblemente debido a una baja activación intra-celular de los nucleósidos

The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population

The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination.España, Ministerio de Economía y Competitividad BIO 2014-51953-

Variación geográfica y social en el panorama lingüístico español

Como es bien sabido, entre las disciplinas lingüísticas que atienden al estudio de las variedades internas de una lengua, ocupan un lugar destacado la Sociolingüística y la Dialectología. Aunque centran su atención en diferentes aspectos del lenguaje, ya que, mientras que la Sociolingüística profundiza en las relaciones entre lengua y sociedad, el ámbito de estudio de la Dialectología es la variación lingüística en diferentes regiones geográficas, podemos afirmar que son campos complementarios que ayudan a abordar el estudio de la variación lingüística. Teniendo en cuenta que los tres tipos de variedades siempre aparecen imbricados en la lengua funcional, no sorprende que las fronteras entre los estudios sociolingüísticos y dialectales sean a menudo difusas. El objetivo de esta colección de trabajos es reunir estos dos campos principales de investigación lingüística. El enfoque de este monográfico tiene una doble vertiente: contribuciones como las de Álvarez, Corts, Louredo o Zuloaga y Atutxa estudian el lenguaje desde un punto de vista diacrónico, mientras que otros, como los de Benito al., Diz, Peralta, Simón o Von Essen, lo hacen desde una perspectiva sincrónica

Self-assembly of a drop pattern from a two-dimensional grid of nanometric metallic filaments

We report experiments, modeling, and numerical simulations of the self-assembly of particle patterns obtained from a nanometric metallic square grid. Initially, nickel filaments of rectangular cross section are patterned on a SiO2 flat surface, and then they are melted by laser irradiation with ∼18-ns pulses. During this time, the liquefied metal dewets the substrate, leading to a linear array of drops along each side of the squares. The experimental data provide a series of SEM images of the resultant morphology as a function of the number of laser pulses or cumulative liquid lifetime. These data are analyzed in terms of fluid mechanical models that account for mass conservation and consider flow evolution with the aim to predict the final number of drops resulting from each side of the square. The aspect ratio, δ, between the square sides' lengths and their widths is an essential parameter of the problem. Our models allow us to predict the δ intervals within which a certain final number of drops are expected. The comparison with experimental data shows a good agreement with the model that explicitly considers the Stokes flow developed in the filaments neck region that lead to breakup points. Also, numerical simulations that solve the Navier-Stokes equations along with slip boundary condition at the contact lines are implemented to describe the dynamics of the problem.Fil: Cuellar Berrio, Ingrith Paola. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Ravazzoli, Pablo Damián. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Diez, Javier Alberto. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Gonzalez, Alejandro Guillermo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Roberts, Nicholas A.. State University of Utah; Estados UnidosFil: Fowlkes, Jason D.. Oak Ridge National Laboratory; Estados UnidosFil: Rack, Philip D.. Oak Ridge National Laboratory; Estados UnidosFil: Kondic, Lou. New Jersey Institute of Technology; Estados Unido

Non-invasive characterization of hybrid gelatin:poly-L-lactide electrospun scaffolds using second harmonic generation and multiphoton imaging

In this work we generated hybrid gelatin:poly-l-lactide electrospun scaffolds and implemented non-invasive methods to characterize them

Does excessive use of smartphones and apps make us more impulsive? An approach from behavioural economics

Purpose Problematic smartphone use has been associated with negative effects in work and school environments. This study proposes the application of a behavioural economics perspective to establish whether heavy smartphone users show a tendency to devalue the consequences of their behaviour in the long term. To address this proposition, the study sought to establish how an objective measurement of usage time of smartphones and apps might help to predict, firstly, participants’ choice behaviour and, secondly, their perceived dependence levels. Design/methodology/approach An objective measurement of the usage time of smartphones and apps was conducted over four weeks (N = 560 data points), and a computer-based intertemporal choice task and the Spanish version of the Smartphone Addiction Inventory (SPAI) were applied. The participants were twenty undergraduate college students. Findings Although the usage time of devices and apps failed to predict the choice behaviour, a correlation was found between the total usage time of smartphones and WhatsApp and Facebook apps and users’ dependence level. On the other hand, dependence had a positive effect on the average selection of the impulsive choice. Originality/value This paper proposes the application of a behavioural economics perspective to explore the relationship between objectively measured usage time of smartphone and apps, choice behaviours in an intertemporal task and users’ perceived dependence levels. This allows us to consider an alternative to the traditional psychiatric approach in an environment of increasing access to and use of mobile digital platforms