丙戊酸钠对戊四氮致痫大鼠海马组织粘着斑激酶表达的影响

目的:探讨抗癫痫药丙戊酸钠（VPA）对戊四氮（PTZ）致痫模型大鼠海马组织中粘着斑激酶（FAK）、FAK-pY397表达水平的影响。方法:将75只大鼠随机分为正常对照组,模型组,VPA低、中、高剂量组(150,300,600 mg·kg-1·d-1) 5组,每组15只。造模大鼠连续腹腔注射戊四氮32 mg·kg-1·d-14周,密切观察大鼠行为学变化。造模成功后,VPA各组给予相应剂量VPA灌胃,连续2周。苏木精-伊红（HE）染色观察大鼠海马组织变化;免疫组化及酶联免疫吸附法（ELISA）检测大鼠外周血清及海马组织中FAK、FAK-pY397及整合素表达情况。结果:与模型组比较,VPA各组癫痫症状有明显缓解,细胞凋亡情况有所改善;免疫组化发现VPA组FAK-pY397的表达明显下降,且随着VPA剂量的加大,FAK-pY397的表达有下降趋势,ITGα3表达组间无显著差异; VPA低、中、高剂量组海马组织中FAK,FAK-pY397及整合素ITGβ1表达水平下降,差异具有显著性（P <0. 05）;外周血清中FAK及整合素ITGβ1蛋白表达水平显著下降（P <0. 05）,但FAK-pY397表达无明显变化。结论:VPA可能通过抑制癫痫大鼠海马组织中FAK-pY397及ITGβ1的表达,参与或影响癫痫过程。漳州市自然科学基金课题(编号:ZZ2017J41

ApoE基因多态性与动脉粥样硬化性脑梗死的相关性研究

目的分析ApoE基因与血脂和颈动脉粥样硬化程度的关系,进一步探讨ApoE基因与动脉粥样硬化性脑梗死的相关性。方法采用病例-对照研究的方法,收集厦门大学附属第一医院神经内科的95例缺血性脑卒中和65例非缺血性脑卒中住院患者。检测患者的ApoE基因型、血脂及颈动脉粥样硬化程度。结果与E2/E3比较,基因型E3/E3、E3/E4可能是缺血性脑卒中的保护因素;缺血性和非缺血性脑卒中患者在血浆TC、LDL-C、ApoB含量比较上均为E3/E4> E3/E3>E2/E3,且2组血浆LDL-C浓度在E3/E4与E2/E3、E3/E3比较中差异有统计学意义(P<0. 05); LDL-C、ApoB与2组颈动脉粥样硬化斑块程度有关,是其危险因素;与基因型E2/E3比较,E3/E4在有斑块组中较无斑块组比例高。结论基因型E3/E3、E3/E4可能是缺血性脑卒中的保护因素。基因型E3/E4可能是导致患者颈动脉粥样斑块形成的危险因素,基因型E3/E4通过影响患者的血浆LDL-C水平进一步导致动脉粥样硬化斑块的形成,但与动脉粥样硬化性脑梗死的相关性研究有待更大的样本量更进一步的研究

The research of the reference value of urinary mercury for 0-6 years old children in coast area

目的通过对厦门市0~6岁儿童尿汞水平调查,了解厦门市儿童汞暴露水平。方法根据行政区划以及儿童人数分布从全市选取代表性的8个社区,进行分层随机抽样,共收集到1076例0~6岁儿童尿样。样本采用随意尿,每个儿童统一使用聚乙烯塑料瓶收集不少于5 M l尿样,尿样统一采用dMA-80自动测汞仪测定。结果儿童尿汞测定值呈偏态分布,因此使用几何平均数表示儿童尿汞的平均水平。男女儿童尿汞几何平均值分别为0.77μg/l和0.74μg/l,经非参数T检验比较,差异无统计学意义(P>0.05)。0~1岁、2~3岁和3~6岁3个年龄段的儿童尿汞几何平均值分别为0.74μg/l、0.75μg/l和0.78μg/l,经非参数T检验比较,差异无统计学意义(P>0.05)。对1076名儿童尿汞值分析得出尿汞的几何平均值为0.76μg/l,95%置信区间0.70~0.81μg/l。结论厦门市0~6岁儿童的尿汞水平的调查结果可以为国内沿海城市0~6岁儿童汞暴露水平的本底值研究提供参考。Objective To understand the mercury exposure level in 0-6 year-old children of Xiamen city through the survey.Methods A total of 1 076 healthy infants and young children aged 0-6 year-old were selected from 8 communities with stratified and cluster sampling method respectively.The spot urine samples of the children were collected( more than 5m L per child).The concentration of mercury was determined by DMA-80 automatic mercy instrument.Results The urine mercury values of the healthy children were in skewed distribution and the results were expressed in geometric mean.The urine mercury values for boys and girls are0.77μg / L and 0.74μg / L,respectively.There was no significant difference for the urine mercury values between boys and girls( P >0.05).Meanwhile,the urine mercury values for groups of 0-1 year-old,2-3 year-old,and 4-6 year-old were 0.74μg / L,0.75μg / L and 0.78μg / L,respectively.The urine mercury values among the three age groups had no significantly difference neither( P > 0.05).The upper 95 th percentile in 1076 urine samples was 0.85 μg / L,95% confidence interval was 0.70-0.81μg / L.Conclusion The average value of 0-6 year-old children's urine mercury level in Xiamen was close to those in the reports of two researches in Shenyang and Germany.Therefore,results will be helpful for the understanding of mercury exposure in 0-6 year-old children of Xiamen city

生物型纳米根管封闭剂的生物学性能研究

目的:对磷酸钙骨水泥为原料制备的生物型纳米根管封闭剂进行体外生物学性能研究,为其临床应用提供依据。方法:致敏性实验:依照GB/T16886.10-2005和GB/T16886.12-2005技术标准测定;细胞毒性实验:依照GB/T16886.5-2003技术标准,采用琼脂扩散法测定;鼠伤寒沙门氏杆菌回复突变试验(Ames试验):依照YY/T0127.10-2001技术标准测定。结果:生物型纳米根管封闭剂无致敏性,不导致染色体畸变,具有轻度细胞毒性。结论:生物型纳米根管封闭剂具有良好的生物学性能

Synthesis of Ru(dpp)_3(ClO_4)_2 doped polyacrylonitrile nanoparticles and its applications in ratiometric pH sensing

采用乳液聚合法制备了掺杂有4,7-二苯基-1,10-邻菲咯啉钌(ru(dPP)3(ClO4)2)的聚丙烯腈纳米颗粒(ru-PAn).经扫描电子显微镜(SEM)表征,制备的ru-PAn的尺寸为135±15 nM,呈规则球型,尺寸分布均匀且在水中的分散性较好.实验考察了氧气、共存离子和PH值对其荧光性质的影响以及其荧光稳定性.以异硫氰根荧光素(fITC)为PH荧光指示剂、ru-PAn为参比信号,初步建立了一种比率荧光PH检测的方法.In this paper Ru(dpp)3(ClO4)2 doped polyacrylonitrile nanoparticles(Ru-PAN) were prepared using emulsion polymerization method.Morphology characterizations of the nanoparticles were performed using scanning electron microscope,the particle diameter was about 135±15 nm.The interference of the oxygen,coexisting ions,pH and photo-stability were investigated.A new ratiometric fluorescence pH sensing system was established via fluorescein isothiocyanate(FITC) as pH-sensitive dye and Ru-PAN as a reference.国家自然科学基金资助项目(20975085

独活寄生汤水提物对退变大鼠椎间盘软骨细胞功能的影响

目的探究独活寄生汤水提物对经IL-1β诱导退变的大鼠椎间盘软骨细胞中Wnt4、GSK-3β、β-catenin、DKK-1的影响。方法采用机械-酶消化法分离大鼠椎间盘软骨组织,进行软骨细胞体外培养、镜下观察与鉴定,分为正常组、模型组(经10 ng/m L浓度IL-1β造模)、实验1组(独活寄生汤水提物组200μg/m L干预24 h)、实验2组(独活寄生汤水提物组200μg/m L干预48 h)。观察4组大鼠椎间盘软骨细胞中Wnt4、GSK-3β、β-catenin、DKK-1 m RNA与蛋白的表达及上清液中Sox 9表达。结果 (1)第2代大鼠椎间盘软骨细胞增殖速度快,呈现多边形,胞核清晰,且含有1~2个核仁,融合后出现\"铺路石\"状,经Ⅱ型胶原法染色后,阳性对照组胞浆区域浸染为棕黄色;(2)与正常组比较,模型组中软骨细胞Wnt 4、GSK-3β、β-catenin m RNA与蛋白表达及上清液Sox 9含量明显提高(P<0.05),DKK-1 m RNA与蛋白表达明显降低(P<0.05);与模型组比较,实验1组、实验2组中软骨细胞Wnt 4、GSK-3β、β-catenin m RNA与蛋白表达及上清液Sox 9含量明显降低(P<0.05),DKK-1m RNA与蛋白表达明显提高(P<0.05),以实验2组变化最为显著(P<0.05)。结论独活寄生汤水提物组可调控退变椎间盘软骨细胞的功能,下调Wnt 4、GSK-3β、β-catenin和上调DKK-1 m RNA与蛋白表达,进而延缓大鼠椎间盘软骨细胞的退变。福建省自然科学基金项目(2017J01224);;福建中医药大学校管课题资助(X2015034

独活寄生汤调控对大鼠椎间盘软骨细胞Wnt/β-catenin信号通路的影响

目的:从Wnt/β-catenin信号通路探讨独活寄生汤水提物对大鼠椎间盘退变软骨细胞功能的影响。方法:（1）用水提加热回流法制备独活寄生汤水提物成分;（2）选取4周龄健康雄性SD大鼠30只,采用机械-酶消化法分离大鼠椎间盘软骨组织,建立软骨细胞体外培养体系并进行鉴定;（3）RT-PCR、We stern blot法分别检测经DKK-1抑制剂干预及（或）经白细胞介素-1β诱导的椎间盘软骨细胞Wnt4、GSK-3β、β-catenin mRNA与蛋白含量的表达。结果:（1）椎间盘软骨细胞经Ⅱ型胶原法染色后,阳性对照组胞浆区域浸染为棕黄色;（2）RT-PCR、Western blot检测结果示,与正常组比较,模型组Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达显著升高（P<0.01）;与模型组比较,独活寄生汤水提物组(100,200,400μg·mL-1)的Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达显著降低（P<0.01或P<0.05）,其中以200μg·mL-1组的表达量最低（P<0.01）。结论:独活寄生汤水提物组可通过调控Wnt/β-catenin信号通路,下调大鼠椎间盘退变关节软骨中Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达,从而延缓椎间盘软骨细胞退变。福建省自然科学基金(2017J01224);;福建中医药大学校管课题(X2015034

肝癌发展中FasL及其受体Fas/DcR3的表达分析

目的研究小鼠肿瘤发生早期肿瘤坏死因子超家族成员FasL及其受体的表达特点,及其与免疫调节相关分子表达的时空关系,探讨其在诱导肿瘤免疫耐受中的作用。方法建立小鼠实体瘤模型,采用RT-PCR方法检测肿瘤组织中可溶型FasL、Fas及DcR3、TGF-β、IL-10在肿瘤发生不同时相的表达,同时,应用ELISA方法定量检测血清中TGF-β、IL-10的表达。结果在早期的肿瘤组织中,Fas的表达先于DcR3,其后DcR3大量表达,而Fas的表达则下调直至丢失,DcR3、FasL同时表达并上调,TGF-β和IL-10也随肿瘤的表达而上调。TGF-β同DcR3的表达具有空间位置的一致性。FasL、DcR3、TGF-β和IL-10的表达水平同肿瘤的生长呈正相关。结论随着肿瘤不断的生长,肿瘤局部的免疫应答逐渐趋向于负调节,FasL、DcR3在诱导肿瘤免疫耐受的过程中可能发挥着重要作用

Electrochemical Impedance Spectroscopic Study of the First Delithiation of Spinel Lithium Manganese Oxide

研究了尖晶石锂锰氧化物电极首次脱锂过程中的电化学阻抗特征.通过选取适当的等效电路拟合实验所得的电化学阻抗谱数据,获得了首次脱锂过程中固体电解质相界面膜(SEI膜)的电阻、电容以及电荷传递电阻、双电层电容等随电极极化电位的变化规律.The first delithiation of the spinel LiMn2O4 electrode was studied using electrochemical impedance spectroscopy (EIS). Appropriate equivalent circuits were proposed to fit the experimental EIS data. Based on the fitting results, the variation of the capacitance and the resistance of SEI(solid electrolyte interphase) film, the resistance of charge transfer, and the capacitance of double layer along with the increase of polarization potential were quantitatively analyzed. The results demonstrated that the resistance and the thickness of the SEI film formed on the spinel LiMn2O4 electrode were both increased with the increase of polarization potential in the first delithiation of the spinel LiMn2O4 electrode; The charge transfer resistance decreases below 4.15 V and increases above 4.15 V, corresponding to the two-step reversible (de)intercalation of lithium between LiMn2O4 and λ-MnO2; The double layer capacitance was influenced by both the state of the spinel LiMn2O4 electrode(different polarization potential) and the two-step reversible (de)intercalation of lithium.国家重点基础研究发展规划项目(2002CB211804)资

Electrochemical impedance spectroscopic study of the first delithiation of spinel lithium manganese oxide

The first delithiation of the spinel LiMn2O4 electrode was studied using electrochemical impedance spectroscopy (EIS). Appropriate equivalent circuits were proposed to fit the experimental EIS data. Based on the fitting results, the variation of the capacitance and the resistance of SEI (solid electrolyte interphase) film, the resistance of charge transfer, and the capacitance of double layer along with the increase of polarization potential were quantitatively analyzed. The results demonstrated that the resistance and the thickness of the SEI film formed on the spinel LiMn2O4 electrode were both increased with the increase of polarization potential in the first delithiation of the spinel LiMn2O4 electrode; The charge transfer resistance decreases below 4.15 V and increases above 4.15 V, corresponding to the two-step reversible (de)intercalation of lithium between LiMn2O4 and lambda-MnO2; The double layer capacitance was influenced by both the state of the spinel LiMn2O4 electrode (different polarization potential) and the two-step reversible (de)intercalation of lithium