A preliminary study on mechanisms of fission yeast Dnt1 participating in accurate chromosome segregation and efficient G2/M transition

在裂殖酵母中，Dnt1主要定位在核仁里，在有丝分裂后期还定位在纺锤体上。本实验室之前的研究发现，Dnt1对于纺锤体检验点的沉默是必需的；另外Dnt1通过下调Wee1蛋白水平调控细胞G2/M转换以及胞质分裂。 本研究通过对染色体分离情况的精确观察，我们发现Dnt1与Monopolin蛋白(Pcs1/Mde4)、AuroraB激酶Ark1分别通过不同途径防止姊妹染色体的merotelicorientation，在染色体准确分离过程中起着重要作用。利用nda3-KM311cdc13-GFPark1-as3纺锤体检验点沉默筛选系统，我们进一步确认Dnt1与SAC的关键组分Bub3在不同的通路上调控检...In the fission yeast Schizosaccharomyces pombe, Dnt1 mainly localizes in the nucleolus, and it also appear on the spindle at anaphase. Our previous studies found that Dnt1 positively regulates G2/M transition and cytokinesis by down-regulating protein levels of Wee1 kinase, Dnt1 is also required for the spindle checkpoint silencing. In this study, by observing chromosome segregation defects duri...学位：理学硕士院系专业：生命科学学院_细胞生物学学号：2162013115252

Research on the Legitimacy of the Practice of Amicus Curiae in the WTO Dispute Settlement System

法庭之友起源于罗马法，原本为“法庭的朋友”，以中立的立场为法庭提供帮助。随着实践的发展，法庭之友的立场逐渐发生改变，演变为“案件当事一方的朋友”，后又发展成为“游说集团之友”。法庭之友实践在美国得到较大的发展，并且由美国联邦最高法院制定了一套指导规则。WTO争端解决机制中法庭之友的实践在一定程度上就受到了美国的影响。除前言和结束语外，本文的正文部分共分三章。第一章介绍法庭之友的历史演变以及在WTO争端解决机制中的实践，阐述上诉机构通过案例逐步确立的法庭之友参与WTO争端解决机制的规则。上诉机构通过美国——虾案、美国——铅和钢铁案对接受和考虑未经要求的法庭之友书状做出了自己的解释，从而一步步地确...The Amicus Curiae originated from Roman law, and it supported the court as a neutral party at that time. Becoming a common practice, it moved from “the friend of court” to “friend of the party” to “friend of the lobbyist”. In the United States, under the principle of “judicial economy” the Supreme Court adopted a set of rules on submission of Amicus Curiae briefs. The experience of the United Stat...学位：法律硕士院系专业：法学院法律系_法律硕士(JM)学号：20030815

A Study about the Issue of China’s Urban and Rural Income Gap since Reform and Opening up

我国城乡居民收入差距问题早已存在。尤其是改革开放以来，随着经济体制改革的深入，我国城乡居民收入快速增长，但差距逐渐拉大，这一问题更加引起了人们越来越多的关注。尤其是近几年城乡居民收入差距的持续扩大不仅制约农村经济发展，而且制约整个国民经济增长。这种非正常的状况会对我国的社会稳定和经济发展产生消极的影响。对于正在向现代化转型的中国来说，城乡居民收入差距的扩大单纯从经济发展方面解释是不够的，它应该是经济发展过程中自然的、人为的、体制的等多方面的因素共同作用的结果。本文通过描述城乡收入差距的现状，分析城乡居民收入差距产生的原因及其影响，探索缩小城乡居民收入差距的对策和途径，对于实现我国经济持续快速健...China's income gap between residents of urban and rural Areas always exists. Especially along with the deepening of economic reform, residents of urban and rural areas have a rapid growth in incomes since reform and opening up, but the gap is gradually widening. More and more people pay attention on this issue. The continued widen income gap between residents of urban and rural areas is not only r...学位：经济学硕士院系专业：经济学院经济研究所_产业经济学学号：2005130269

Skin Detection Technology with Fusion Approach

皮肤检测技术发展迅速,并且能够应用于检测和跟踪人体部位、计算机视觉和可视化等多个领域。然而,皮肤检测的主要困难仍然是不同程度的皮肤色调、光照条件和颜色接近肤色的背景等等。本文研究了一种新奇的基于融合策略下的动态皮肤检测,它是由一个平滑动态二维直方图、高斯混合模型和基于脸部皮肤色调颜色计算的实时动态阈值这三种检测方法融合在一起的。本研究通过人脸检测来强化肤色模型,这是因为人脸是不同色调的皮肤颜色的一个突出特征,尤其是在包含不同种族的多个人脸图像中。定性和定量实验结果表明,该方法由于其较低的计算成本和较高的精确度,比目前先进检测技术更稳定有效。The skin detection technology develops rapidly and is capable for a wide range of applications in many fields, such as detect and track the components of human body, computer vision and visualization.However,the principal obstacles faced by skin detection are still the different degrees of skin tone color, illumination conditions and skin color-like backgrounds.This paper proposes a novel skin dynamic detection based on fusion strategy, which fuses a smoothed 2-D histogram, Gaussian model and an online dynamic threshold based on the calculation of face skin tone color.In this research, we adopt face detector to refine the skin model, because face is a prominent indicator of different characteristics of skin tone color, especially in images that include more than one face with different ethnicity.Qualitatively and quantitatively experimental results show that the proposed method is more robust and effective compared to state-of-the-art methods, owing to its low computational costs and high accuracy

Identification of Polygonatum from Wuyishan and Its Surrounding Areas by ISSR Molecular Markers and Their HPLC Fingerprints

采用ISSR分子标记鉴定方法及HPLC色谱方法对武夷山及周边地区5份黄精植物样本进行DNA分子标记鉴定及指纹图谱分析,并通过聚类分析软件分别探讨其遗传相关聚类图谱。ISSR分子标记法能较好地对5份黄精植物样本进行区分,HPLC指纹图谱分析同样表明5份黄精样本化学成分存在一定的差别。DNA分子标记及HPLC指纹图谱分析的遗传相关聚类分析结果相似,表明武夷山及周边地区的野生黄精种质资源存在明显的差别。The purpose of this project was to identify and analyze the genetic relationships of 5 kinds of Polygonatum in Wuyishan and its surrounding areas. Using ISSR marker and HPLC molecular identification method for chromatographic methods analysis of DNA markers for identification and fingerprinting of 5 Polygonatum samples, the genetic correlation map of Polygonatum was studied by cluster analysis software. ISSR molecular marker method could be used to distinguish Polygonatum of Wuyishan and its surrounding areas. HPLC fingerprint analysis also showed that there were some differences in the composition of Wuyishan and its surrounding distribution. The results of genetic correlation cluster analysis were similar by ISSR and HPLC. There were obvious differences in the germplasm resources of Wuyishan and its surrounding areas from the DNA molecular markers and HPLC fingerprinting analysis results.福建省科技厅自然科学基金重点项目（2013Y0018）;福建省科技厅自然科学基金青年项目（2015J01065

赋能教育模式对血液透析患者钙磷代谢及营养状态的影响

目的观察赋能教育模式对血液透析伴矿物质及骨代谢异常患者钙磷代谢及营养状态的影响。方法组建赋能教育模式团队,选择2015年6月至2016年6月我院透析中心维持性血液透析(maintenance hemodialysis,MHD)患者60例,赋能教育模式干预12周后,采用自身前后对照的方法,比较患者血钙、血磷、钙磷乘积、全段甲状旁腺激素(intact parathyroid hormone,i PTH)、血红蛋白(hemoglobulin,Hb)、血浆白蛋白(albumin,Alb)、前白蛋白(pre albumin PAB)、肱三头肌皮褶厚度(triceps skinfold thickness,TSF)和上臂中段肌肉周径(mid-arm muscle circumference,MAMC)。结果赋能教育模式后患者血钙、血磷、钙磷乘积、赋能教育模式后患者血钙(t=0.017,P=0.002)、血磷(t=0.368,P=0.005)、钙磷乘积(t=3.089,P≤0.001)、i PTH降低(t=4.216,P=0.004),Hb(t=-1.267,P=0.039)、Alb升高(t=-1.051,P=0.045),TSF升高(t=-2.546,P=0.009),MAMC有升高趋势,但差异尚无统计学意义(t=-1.056,P=0.736)。结论赋能教育模式可改善血液透析伴矿物质及骨代谢异常患者钙磷代谢及营养状态。国家自然科学基金项目(No:71403232);;厦门市科技计划项目(No:3502Z20174037

Effects of the Xitongxiao prescription on apoptosis and proliferation of chondrocytes in knee

目的：探讨膝痛消方对促进软骨细胞增殖和抑制IL-1β诱导的软骨细胞凋亡的作用。方法：体外培养膝关节软骨细胞，用阿利新蓝染色方法检测软骨细胞蛋白多糖，用Ⅱ型胶原免疫荧光染色检测软骨细胞Ⅱ型胶原的表达，以鉴定软骨细胞；用MTS检测软骨细胞的增殖；用IL-1β诱导软骨细胞凋亡，AnnexinV/PI双染检测细胞凋亡。结果：膝关节剥离得到的细胞形态为多角形或梭形，阿利新蓝与Ⅱ型胶原免疫荧光染色均呈阳性，证明分离培养的细胞为软骨细胞。MTS实验结果发现膝痛消方提取物处理软骨细胞后检测到的OD值随浓度增加而增加且显著高于对照组。此外，IL-1β处理诱导软骨细胞凋亡，而膝痛消方提取物能够有效地抑制IL-1β引起的细胞凋亡。结论：膝痛消方能促进软骨细胞的增殖，抑制IL-1β诱导的软骨细胞的凋亡。以上发现为膝痛消方临床上治疗膝关节骨性关节炎提供药理作用依据。Objective:To investigate the effects of the Xitongxiao prescription on the cell proliferation and the inhibition of IL-1β-induced apoptosis of chondrocytes.Methods:To identify chondrocytes,the articular chondrocytes were cultured in vitro;staining with alcian blue was used to detect the protein polysaccharide,and staining with collagen immunofluorescence was used to examine the expression of type II collagen in chondrocytes.The proliferation of chondrocytes was determined by MTS.Apoptosis of chondrocytes was induced by IL-1β,and the effect of apoptosis was tested by Annexin V/PI double staining.Results:The morphology of the cells obtained from the knee joint was polygonal or fusiform.The tested results of Alcian blue and collagen immunofluorescence staining were positive.These together certified that the isolated cells were knee chondrocytes.MTS assay results showed that the detected OD values were dosedependently increased and they were significantly higher than those of the control group.In addition,the apoptosis of chondrocytes was induced by IL-1βtreatment.While the extract of the Xitongxiao prescription could effectively inhibit the IL-1β-induced apoptosis of chondrocytes.Conclusion:The Xitongxiao prescription could promote the proliferation of chondrocytes and inhibit the apoptosis of chondrocytes induced by IL-1β.The above findings may provide one of pharmacological mechanisms of the Xitongxiao for clinical treatment of knee osteoarthritis.厦门市科技计划项目(3502z20144031);福建省自然科学基金计划资助项目(2015J01065)

厦门市思明区老年人对医养结合养老模式的认知和参与意愿现状及其影响因素

目的:调查厦门市思明区老年人对医养结合养老模式的认知及参与意愿,分析其影响因素,为推进医养结合养老工作的发展提供参考。方法:采用方便抽样方法,对户籍在厦门市且居住在思明区的60周岁及以上的114名老年人进行问卷调查。结果:目前厦门市思明区老年人对医养结合养老模式的认知处于较低水平,参与意愿偏低。年龄、文化程度、子女、了解程度是影响老年人医养结合养老模式参与意愿的因素,其中文化程度、了解程度是影响参与意愿的主要因素。结论:养老机构应采取形式多样的宣传教育,提高老年人对医养结合养老模式的了解程度及参与意愿,推进医养结合养老产业的发展

温带荒漠中温度和土壤水分对土壤呼吸的影响

荒漠对气候变化具有高度敏感性,深刻认识和量化非生物因子对荒漠生态系统土壤呼吸的影响具有重要意义。采用自动CO2通量系统(Li-8100)监测了梭梭(Haloxylon ammodendron)、假木贼(Anabasis aphylla)和盐穗木(Halostachys caspica)群落生长季土壤呼吸及温度、土壤含水量等,深入分析了水热因子对土壤呼吸的影响。土壤呼吸具有不对称的日格局,最小值出现在8:00,最大值在12:00～14:00。土壤呼吸的季节格局与气温变化基本同步,最小值在生长季末期(10月),最大值在生长季中期(6～7月)。梭梭、假木贼和盐穗木群落生长季平均土壤呼吸速率分别为0.76、0.52和0.46μmol CO2·m-2·s-1。气温对假木贼(51%)和盐穗木群落(65%)土壤呼吸季节变化的解释率高于梭梭(35%)。梭梭、假木贼和盐穗木群落土壤呼吸温度敏感性(Q10)逐渐增大,基础呼吸速率(R10)逐渐减小。剔除温度影响后,梭梭、假木贼群落土壤呼吸与土壤含水量呈显著的幂二次方函数关系,盐穗木群落两者关系却明显减弱,未达到显著水平。气温、土壤含水量的二元方程均能解释群落土壤呼吸大部分的时间变异:梭梭群落71%～93%、假木贼群落79%～82%、盐穗木群落70%～80%。人工模拟降水后土壤呼吸速率表现出降水后10min减小、180min时明显增加、达到最大值后再次衰减的现象。5和2.5mm降水处理下的土壤呼吸速率最大值和其后的递减值高于对照处理,土壤呼吸增加、达到峰值和其后递减过程与5cm土壤温度变化基本同步

少穗竹和四季竹竹笋的营养成分分析

对福建省华安竹种园2种少穗竹属笋用竹少穗竹和四季竹竹笋的基本营养成分进行测定分析。结果表明:四季竹竹笋的含水量、灰分、脂肪含量高于少穗竹,但蛋白质含量低于少穗竹;而少穗竹竹笋氨基酸、必需及半必需氨基酸、呈味氨基酸总含量高于四季竹。厦门市科学技术局科研项目(编号:3502Z20102003和3502Z20144072