Cloning and Functional Analysis of DlIKU1 Gene in Dimocarpus longan Lour.

拟南芥(Arabidopsis thaliana)IKU1基因突变可以产生较小的种子.根据植物IKU1同源基因的高度保守性,设计引物,PCR扩增获得龙眼(Dimocarpus longan Lour.)同源基因Dl IKU1的编码序列,其编码的蛋白含有VQ保守基序,与其他物种中同源蛋白的氨基酸序列存在较高的同源性;由于龙眼遗传转化的限制,构建Dl IKU1基因的超量表达载体并转化拟南芥iku1突变体,统计转基因植株子代种子的长宽变化,结果表明:龙眼Dl IKU1基因的超量表达可以显著增加拟南芥种子的大小,说明龙眼Dl IKU1基因可以影响种子发育.上述结果为研究龙眼种子发育机理提供了理论和实验依据,有助于龙眼产业的发展.IKU1 gene mutation in Arabidopsis thaliana produced small seeds.The primers were designed according to the highly conservative sequence of IKU1 homologous genes. The longan DlIKU1 gene was obtained via PCR amplification. The amplified sequences of Longan DlIKU1,990 bp,contained VQ motif,and had high homology with the other species.Because of low efficiency of the longan genetic transformation,we constructed the overexpression vector of DlIKU1 gene for genetic transformation in Arabidopsis. Then we summarized changes in length and width of the progeny seed to study the function of DlIKU1 gene. The results showed that DlIKU1 gene might affect seed development to a certain extent.This study provided theoretical and experimental basis for the development of longan seed,and it will be helpful to the development of longan industry.农业科研杰出人才及其创新团队项目(20120911);; 国家产业技术体系项目(CARS-33-05);; 福建省自然科学基金(2014J01100

龙眼APETALA1(AP1)同源基因的克隆与序列分析

根据植物APETALA1(AP1)同源基因的高度保守性,设计合成一对特异引物,从龙眼基因组DNA中扩增出一条长400bp左右的基因片段,插入到pMD-T载体中。测序后分析表明,扩增得到了龙眼AP1同源基因片段。该片段序列包含两个内含子(长96bp和165bp),编码区编码36个氨基酸。与其它植物AP1同源基因的相应区域氨基酸序列具有较高的同源性,其中与花椰菜AP1基因同源性高达91%,与欧亚种葡萄、苹果、柑橘、枇杷的AP1基因同源性都在80%以上。福建省农业科技重大专项资助项目“闽台果茶良种选育研究及产业化”(2004NE02-1

枇杷花黄酮提取液缓解小鼠肠道运动障碍的作用

以枇杷(Eriobotryajaponica Lindl.)花黄酮提取液(EjFF)为受试物,采用复方地芬诺酯建立小鼠便秘模型,通过小鼠小肠推进实验和排便实验,评估其对小鼠的首粒排便时间、5h内排便粒数和排便量、小肠墨汁推进率以及小鼠结肠突触素(SY)含量的影响.结果显示:EjFF不影响小鼠体质量,但能显著缩短小鼠排便时间,增加5h内排便粒数和排便量,提高小肠墨汁推进率,并提高小鼠结肠SY含量(p<0.05).上述结果表明EjFF可以有效缓解小肠运动障碍.福建省农业科学院青年英才计划(C2017-04);;\n农业部物种品种资源保护(热带作物)项目(151721301354051701);;\n国家农作物种质资源共享服务平台项目(NICGR2018-054

枇杷花黄酮提取液缓解小鼠肠道运动障碍的作用

植物源功效成分具有多种生物活性且副作用小,日益成为缓解肠道运动障碍研究的热点。以枇杷花黄酮提取液为受试物,采用复方地芬诺酯建立小鼠便秘模型,通过小鼠小肠推进实验和排便实验,评估小鼠的首次排便时间、5 h内排便粒数、排便量、小肠墨汁推进率以及对小鼠结肠突触素(SY)的影响。结果显示:枇杷花黄酮提取液不影响试验小鼠体重正常发育。与模型组相比,枇杷花黄酮提取液能显著缩短小鼠排便时间(P<0.05),增加排便粒数(P<0.05)和排便重量(P<0.05),提高小肠墨汁推进率(P<0.05,P<0.01),并提高小鼠结肠SY含量(P<0.05)。上述结果表明枇杷花黄酮提取液可以有效缓解小肠运动障碍,为后续枇杷花的功能化开发利用提供参考。福建省农业科学院青年英才计划(C2017-04);;农业部物种品种资源保护(热带作物)项目(151721301354051701);;国家农作物种质资源共享服务平台(NICGR2018-054

RAPD Analysis on 24 Longan Germplasm Resources

利用RAPD技术对原产福建20份、广东1份、四川3份,共24份龙眼种质资源进行分析.从100条引物中筛选出14条多态性引物,共扩增出122条谱带,多态性谱带共88条,多态性比例72.1%.对RAPD结果进行聚类分析,根据相似系数0.28的水平可将24份龙眼品种(系)分为3类.供试各品种多态性比例介于0.12和0.28之间,揭示福建本地品种的遗传多样性不太丰富.In this study,14 primers were selected from 100 random primers and could be successfully used for amplifying the DNA of 24 longan germplasm resources(among them 20 from Fujian,1 from Guangdong and 3 from Sichuang).122 bands were obtained,among them 88 bands show polymorphism,amounting to 72.1%.The DPS dendrogram of 24 resouuces were divided into 3 groups at the level of the similarity coefficient of 0.28.The polymorphic percentage of all 24 test resouces was from 0.12 to 0.28,the results indicated that these Fujian longan resouces were not with abundant genetic diversity.国家科技支撑计划(2007BAD07B01);; 国家科技基础条件平台项目(2005DKA21002-27);; 福建省财政专项(STIF-Y06)资

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies