Implementation of License File Scheme Through Modified Digital Signature Scheme Based on PKI

基于软件的保护方式主要有注册码和许可证文件,文章介绍了将单向散列函数同公开密钥相结合实现数字签名的技术,提出了一种软件许可证生成、验证的许可系统,并基于gnu开源库lIbgCryPT为电信设备实现许可证系统。与传统技术相比,该系统具有简单易用、安全可靠的特点。There are two main methods,i.e.License Key and License File in software copyrights protection area based on soft mode.This paper constructed digital signature adopting public key cryptography technique and Hashing,and then proposed a new license flie generation and validation system,and also gave detailed design and implemention based on Libgcrypt which belongs to GNU.This system is easier and safer to use

多色探针熔解曲线法在卡马西平不良反应相关基因检测中的临床评价

目的对多色探针熔解曲线法(multicolor melting curve analysis,MMCA)用于卡马西平不良反应相关的HLA-B*15:02基因检测进行临床评价。方法收集厦门市中心血站1 147份厦门地区无偿献血者的外周静脉血样本,经基因DNA提取后,按双盲对照试验,分别应用MMCA法和HLA-SBT测序法对各样本进行HLA-B*15:02基因检测,比较2种检测方法的符合率。对于检测结果不一致的标本,采用第三方Sanger测序和电泳验证,计算总符合率。结果采用MMCA法共检出77份HLA-B*15:02阳性标本,1 070份HLA-B*15:02阴性标本。采用HLA-SBT测序法共检出74份HLA-B*15:02阳性标本,1 070份HLA-B*15:02阴性标本,以及3份无明确的分型信息的标本(仅显示为B*15:VG-B*15:CYS型)。该3份标本经Sanger测序以及电泳验证,确认为HLA-B*15:02阳性标本。因此,MMCA法检测HLA-B*15:02基因的阳性检出率为100%(77/77),阴性检出率为100%(1 070/1 070),总符合率为100%(1 147/1 147)。此外,在1 147份临床标本中共检出77份阳性结果,HLA-B*15:02的携带率为6.7%(77/1147),这与文献报道的数据基本一致。结论 MMCA法用于HLA-B*15:02基因的检测,具有简便、快速、灵敏度高、特异性强等优点,可应用于卡马西平不良反应相关的HLA-B*15:02基因的临床辅助检测

Detection of incoherent broadband terahertz light using antenna-coupled high-electron-mobility field-effect transistors

The sensitivity of direct terahertz detectors based on self-mixing of terahertz electromagnetic wave in field-effect transistors is being improved with noise-equivalent power close to that of Schottky-barrier-diode detectors. Here we report such detectors based on AlGaN/GaN two-dimensional electron gas at 77~K are able to sense broadband and incoherent terahertz radiation. The measured photocurrent as a function of the gate voltage agrees well with the self-mixing model and the spectral response is mainly determined by the antenna. A Fourier-transform spectrometer equipped with detectors designed for 340, 650 and 900~GHz bands allows for terahertz spectroscopy in a frequency range from 0.1 to 2.0~THz. The 900~GHz detector at 77~K offers an optical sensitivity about $1~\mathrm{pW/\sqrt{Hz}}$ being comparable to a commercial silicon bolometer at 4.2~K. By further improving the sensitivity, room-temperature detectors would find applications in active/passive terahertz imaging and terahertz spectroscopy.Comment: 4.5 pages, 5 figure

Calibration and Preliminary Application of Linear Quantitation Standard for Anti-HEV IgG Antibody

目的建立抗戊型肝炎病毒(Anti-HEV)IgG抗体的定量线性标准品,并进行初步应用。方法利用抗-HEV IgG和抗-HEV IgM ELISA检测试剂筛选出1份抗-HEV IgG阳性血清L9,经基因1型和4型的HEV ORF2C-端抗原及239抗原进行Western blot确认后,用WHO定量标准品,由3个实验室协作标定,利用量反应平行线法计算其抗-HEV IgG的含量。考察已标定的L9血清的稳定性,并用所标定的1.5倍系列稀释的血清对国内外6家抗-HEV IgG试剂的灵敏度进行检测。选择一灵敏度较高的试剂,在其线性范围内取L9的5个稀释度作为抗-HEV IgG抗体定量线性标准,对高、中、低浓度的3份临床血清重复检测5次,考察其重复性;对实验感染猴的系列血清中抗-HEV IgG含量进行定量检测,考核该定量线性标准品的应用效果;并对每次定量试验中的线性方程进行分析,确定相关系数r值和斜率k值的范围。结果经国内外试剂检测筛选出的阳性血清L9与基因1型和4型的HEV ORF2 C-端抗原及239抗原均有阳性反应。经协作标定,L9血清抗-HEV IgG含量为16.9U/ml。L9血清在-20℃下保存6、12、18个月,2～8℃保存24、48、96h后,定量结果均在95%置信区间内,且抗-HEV IgG含量均未明显下降。6家抗-HEVIgG检测试剂灵敏度差异较大,范围为0.03～5.00U/ml。确定L9血清从0.42U/ml开始的5个1.5倍系列稀释度,作为某一试剂抗-HEVIgG抗体定量线性标准品。利用该线性定量标准检测高、中、低浓度的3份临床血清,定量结果重复性较好;对实验感染猴系列血清进行定量检测,结果可有效地反映抗体水平变化趋势;94%的定量检测试验,r≥0.98,1.15≥k≥0.95。结论已建立了抗-HEVIgG抗体定量线性标准品,可用于疫苗免疫原性评价和流行病学调查。Objective To develop a linear quantitation standard for anti-hepatitis E virus (HEV) IgG antibody. Methods An anti-HEV IgG positive serum sample L9 was screened by using Anti-HEV IgM ELISA kit and Anti-HEV IgG ELISA kit and confirmed by Western blotting with HEV ORF2 C-terminal antigen of genotypes 1 and 4 and 239 antigen, then calibrated by 3 laboratories using WHO quantitation standard. Calculate the anti-HEV IgG content by dose-response parallel line assay, and evaluate the stability of calibrated serum sample. The sensitivities of 6 domestic and imported anti-HEV IgG ELISA kits were evaluated with the calibrated serum sample diluted 1. 5-fold serially. A linear quantitation standard for anti-HEV IgG antibody consisted of 5 dilutions of L9 within the linear determination range of a highly sensitive kit and evaluated for reproducibility by repeat test for 3 clinical serum samples, at high, moderate and low anti-HEV IgG contents respectively, for 5 times. The anti-HEV IgG contents in serum samples of HEV-infected monkeys were determined by the standard, and the determination curves were analyzed to define correlation coefficient r and slope k. Results Serum sample L9 showed positive reaction with HEV ORF2 C-terminal antigen of genotypes 1 and 4 and 239 antigen, and its anti-HEV IgG content was calibrated as 16. 9 U / ml. After storage at -20℃ for 6, 12 and 18 months or at 2 ～ 8℃ for 24, 48 and 96 h, all the quantitative determination results were within the 95% CI, and anti-HEV IgG content showed no significant decrease. The sensitivities of 6 kits evaluated with the L9 ranged from 0. 03 to 5. 00 U / ml. The linear quantitation standard for anti-HEV IgG antibody consisted of 5 dilutions of L9, starting from a concentration of 0. 42 U / ml. The determination results of 3 clinical serum samples showed good reproducibility of the standard. The determination results of sera of HEV-infected monkeys reflected the change of antibody level effectively. The r values of 94% of quantitative determination curves were not less than 0. 98, and the k values ranged from 1. 15 to 0. 95. Conclusion A linear quantitation standard for anti-HEV IgG antibody was established, which was suitable for the evaluation of immunogenicity and epidemical investigation of vaccine

Interfacial Effects in Iron-Nickel Hydroxide–Platinum Nanoparticles Enhance Catalytic Oxidation

该研究工作是在郑南峰教授的领导下，由校内外、国内外多个课题组共同努力，历时三年完成。郑南峰、傅钢、陈明树等三个课题组紧密协作负责催化剂的合成、表征、性能测试以及催化机理研究；中国科学院物理研究所谷林研究员主要负责纳米颗粒的亚埃级球差校正高分辨透射电子显微研究；加拿大达尔豪斯大学化学系的张鹏教授课题组和台湾同步辐射研究中心李志甫研究员等参与催化剂的同步辐射X-射线吸收光谱研究。 该工作受到了国家自然科学基金委、科技部、厦门大学、固体表面物理化学国家重点实验室、能源材料化学协同创新中心以及醇醚酯化工清洁生产国家工程实验室的资助与支持。Hybrid metal nanoparticles can allow separate reaction steps to occur in close proximity at different metal sites and accelerate catalysis. We synthesized iron-nickel hydroxide–platinum (transition metal-OH-Pt) nanoparticles with diameters below 5 nanometers and showed that they are highly efficient for carbon monoxide (CO) oxidation catalysis at room temperature. We characterized the composition and structure of the transition metal–OH-Pt interface and showed that Ni2+ plays a key role in stabilizing the interface against dehydration. Density functional theory and isotope-labeling experiments revealed that the OH groups at the Fe3+-OH-Pt interfaces readily react with CO adsorbed nearby to directly yield carbon dioxide (CO2) and simultaneously produce coordinatively unsaturated Fe sites for O2 activation. The oxide-supported PtFeNi nanocatalyst rapidly and fully removed CO from humid air without decay in activity for 1 month

Semantic Memory in Patients with Mild Cognitive Impairment

轻度认知损伤(Mildcognitive impairment,MCI)是介于正常老化与痴呆之间的过渡阶段,表现为与年龄和教育程度不相称的认知功能减退。本文回顾了近期有关MCI语义记忆的研究,分析比较了各研究的实验任务及结果,得出MCI患者存在一定程度的由多种原因造成的语义记忆损伤,语义记忆测验对MCI的早期筛查和转归预测有重要作用。最后指出将来的研究需要将包括语义记忆成分在内的多种认知测验相结合,以便及早发现有可能发展为痴呆的危险个体并开展相应干预

轻度认知损伤的语义记忆研究述评

轻度认知损伤(Mildcognitive impairment,MCI)是介于正常老化与痴呆之间的过渡阶段,表现为与年龄和教育程度不相称的认知功能减退。本文回顾了近期有关MCI语义记忆的研究,分析比较了各研究的实验任务及结果,得出MCI患者存在一定程度的由多种原因造成的语义记忆损伤,语义记忆测验对MCI的早期筛查和转归预测有重要作用。最后指出将来的研究需要将包括语义记忆成分在内的多种认知测验相结合,以便及早发现有可能发展为痴呆的危险个体并开展相应干预

中国心理学会会议论文集

目的:遗忘型轻度认知功能障(a MCI)碍通常被认为是介于正常老化和临床痴呆之间的过渡阶段,表现为与年龄和教育程度不相称的认知功能减退,但尚未达到痴呆诊断标准。情绪性的材料比中性材料更容易记忆,这种现象被称作&quot;情绪记忆优势效应&quot;。青年人和老年人都存在情绪记忆优势,然而a MCI的相关研究却发现了矛盾的结论:在使用回忆测验(recall)的任务中,a MCI表现出了正常的情绪记忆优势效应,而在使用再&nbsp;</p

Neural Networks-Intergrated Artificial Life: a New Approach to Study Functional Neural Circuit

基于设计能够自动挑选出有特定功能的模拟神经回路这一设想,通过给虚拟生物体一个未连接的模拟小神经群,在与虚拟环境的相互作用下,研究其神经网络的拓扑结构的演化。具体做法是:让虚拟生物体的小神经群在最初视觉神经连接至中枢神经,中枢神经内部无相互连接,中枢神经与运动神经不相连接,运动神经元可以有一定概率的不通过中枢神经而随机激发。然后在虚拟环境中投入食物,人工生物体运动神经元自动激发造成食物与生物体相对位移的改变,进而导致Hebb学习,造成神经网络拓扑结构的变化。最后给出了仿真结果的理论意义以及模型进一步完善的可能性。Study the topological structure evolution of neural networks,by assigning an unconnected simulated small neural groups to interact with the vitural environment. In detail,first,the virtual life's small neural group connected with the central neural where no inter-connection,and no connection between central neural and moving neurons which are activated probably,the food are added to the virtual environment.Moving neurons are auto-activited such that relative move produced between life and food,thus lead to Hebb learning and topological structure change of neural network.Finally,the oretical meaning of simulation results and further improvement of the model is discussed

对违法取证行为的控制——以侦查程序构造为视角

对以刑讯逼供为其典型代表的违法取证行为的防范与控制,是理论界与实务界共同关注的问题。违法取证行为古已有之,且有屡禁不止的趋势。认真透彻地分析违法取证行为存在的原因是有效控制这一行为的前提和基础。通过对侦查程序构造现有缺憾的改造,实现权力与权利的双重制约,达到控制违法取证行为的目标