干扰素防治草鱼出血病的效果初探

对人工诱导的鲫鱼干扰素防治草鱼出血病的效果进行了初步探讨。结果表明 ,人工诱导的鲫鱼干扰素确能有效防治草鱼出血病 ,腹腔注射和药浴浸泡两种途径均有一定效果。在病毒感染前使用干扰素处理的效果好于在病毒感染后使用干扰素处理。多次使用干扰素处理的效果好于一次性处

DEFORMATION MONITORING of BULKY BACKFILL IN NO.2 MINE AREA IN JINCHUAN

针对金川二矿区大体积充填体稳定性问题,简要论述近年来地表岩移和井下地压显现特征,并开展大体积充填体的变形机制研究。制定针对大体积充填体变形的监测方案:采用自制的变形监测装置对1 150~1 200 M水平50 M高充填体的相对变形进行监测,采用水准仪对大体积充填体和围岩体的下沉变形进行量测。监测结果表明,大体积充填体综合下沉变形速率为16.00~51.44 MM/月,最大累计下沉量达515 MM,大体积充填体自身相对变形速率为1.953~28.585 MM/月,最大累计相对变形为285.85 MM。进一步分析大体积充填体综合变形、自身相对变形和围岩体变形的发展趋势。研究成果对于深刻认识二矿区大体积充填体的稳定性、围岩体的变形等提供了重要的依据。The characteristics of surface subsidence and ground pressure in recent years in No.2 mine area,Jinchuan were briefly described and the mechanism of deformation was carefully analyzed.A monitoring scheme was implemented based on the results of deformation analysis.A device was made to monitor the relative deformation of bulky backfill of 50 m in height at the altitude level of 1 150 to and 1 200 m.Bubble level was used to monitor the vertical displacement of the bulky backfill and the surrounding rock.The monitoring results shows that the rate of total deformations of measurement points is 16.00 – 51.44 mm/month and that the maximum accumulated deformations is 515 mm.The rate of relative deformations at measurement points is1.953 – 28.585 mm/month, and the maximum accumulated relative deformations is 285.85 mm.The development trends of the total deformation,the relative deformation and the deformation of surrounding rock are also studied

兔肾积水铸型标本设计与制作

管道铸型标本制作已有几十年的历史,真正发展始于70年代末期,近20年来经过不断的探索,对于人的器官、肢体和某些器官联合管道的铸型方法已有成熟的

Design of Ultra-wideband Printed Rectangular Monopole Antenna

针对超宽带通信应用,研究影响印制单极天线阻抗带宽的主要因素,设计基于微带馈电的小型化印制矩形单极天线。按照等效性原理,采用黄金分割比设计矩形振子体;通过接地面上端引入渐变梯形或凹形结构,同时调整馈入端接地面间隙,可实现印制矩形单极天线的超宽带特性。对具有渐变梯形或凹形接地面结构的微带馈电矩形印制单极天线结构进行优化,仿真结果表明,前者的阻抗带宽为2.96~17.94gHz,后者的阻抗带宽为2.9~13.3gHz,而两者的辐射方向基本保持不变。实测结果与仿真结果基本一致,达到了超宽带通信应用的要求。To achieve ultra-wideband(UWB)radio applications,the microstrip-fed printed rectangular monopole antenna is analyzed and devised.Using golden section ratio the rectangular monopole is of layout based on equivalent principle.To maximize the impedance bandwidth,it is necessary introducing the taper or concave ground plane and feed gap of microstrip line.The simulated results of the optimized-size antennas show that the impedance bandwidth defined by return loss less than-10dB is from 2.96GHz to 17.94GHz with a ratio of about 6.06∶1 for taper ground antenna and from 2.9GHz to 13.3GHz with a ratio of about 4.59∶1 for concave ground antenna.At the same time a nearly omnidirectional radiation pattern is exhibited.These results are confirmed by the experimental test and these compact size antennas are suitable for various wideband applications.福建省科技计划重点资助项目(2007H0036

独活寄生汤水提物对退变大鼠椎间盘软骨细胞功能的影响

目的探究独活寄生汤水提物对经IL-1β诱导退变的大鼠椎间盘软骨细胞中Wnt4、GSK-3β、β-catenin、DKK-1的影响。方法采用机械-酶消化法分离大鼠椎间盘软骨组织,进行软骨细胞体外培养、镜下观察与鉴定,分为正常组、模型组(经10 ng/m L浓度IL-1β造模)、实验1组(独活寄生汤水提物组200μg/m L干预24 h)、实验2组(独活寄生汤水提物组200μg/m L干预48 h)。观察4组大鼠椎间盘软骨细胞中Wnt4、GSK-3β、β-catenin、DKK-1 m RNA与蛋白的表达及上清液中Sox 9表达。结果 (1)第2代大鼠椎间盘软骨细胞增殖速度快,呈现多边形,胞核清晰,且含有1~2个核仁,融合后出现\"铺路石\"状,经Ⅱ型胶原法染色后,阳性对照组胞浆区域浸染为棕黄色;(2)与正常组比较,模型组中软骨细胞Wnt 4、GSK-3β、β-catenin m RNA与蛋白表达及上清液Sox 9含量明显提高(P<0.05),DKK-1 m RNA与蛋白表达明显降低(P<0.05);与模型组比较,实验1组、实验2组中软骨细胞Wnt 4、GSK-3β、β-catenin m RNA与蛋白表达及上清液Sox 9含量明显降低(P<0.05),DKK-1m RNA与蛋白表达明显提高(P<0.05),以实验2组变化最为显著(P<0.05)。结论独活寄生汤水提物组可调控退变椎间盘软骨细胞的功能,下调Wnt 4、GSK-3β、β-catenin和上调DKK-1 m RNA与蛋白表达,进而延缓大鼠椎间盘软骨细胞的退变。福建省自然科学基金项目(2017J01224);;福建中医药大学校管课题资助(X2015034

独活寄生汤调控对大鼠椎间盘软骨细胞Wnt/β-catenin信号通路的影响

目的:从Wnt/β-catenin信号通路探讨独活寄生汤水提物对大鼠椎间盘退变软骨细胞功能的影响。方法:（1）用水提加热回流法制备独活寄生汤水提物成分;（2）选取4周龄健康雄性SD大鼠30只,采用机械-酶消化法分离大鼠椎间盘软骨组织,建立软骨细胞体外培养体系并进行鉴定;（3）RT-PCR、We stern blot法分别检测经DKK-1抑制剂干预及（或）经白细胞介素-1β诱导的椎间盘软骨细胞Wnt4、GSK-3β、β-catenin mRNA与蛋白含量的表达。结果:（1）椎间盘软骨细胞经Ⅱ型胶原法染色后,阳性对照组胞浆区域浸染为棕黄色;（2）RT-PCR、Western blot检测结果示,与正常组比较,模型组Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达显著升高（P<0.01）;与模型组比较,独活寄生汤水提物组(100,200,400μg·mL-1)的Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达显著降低（P<0.01或P<0.05）,其中以200μg·mL-1组的表达量最低（P<0.01）。结论:独活寄生汤水提物组可通过调控Wnt/β-catenin信号通路,下调大鼠椎间盘退变关节软骨中Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达,从而延缓椎间盘软骨细胞退变。福建省自然科学基金(2017J01224);;福建中医药大学校管课题(X2015034

Determination of five poisonous elements in holothurian sold on the market by Microwave Digestion with ICP—MS

建立了微波消解—电感耦合等离子体质谱法测定海参中Cu,AS,Cd,Hg,Pb 5种有毒元素含量的方法,采用In元素为内标,利用其回收率对以上5种元素的测定结果进行校正,该方法变异系数(CV%)为0.2%--4.0%,In元素加标回收率为96.7%--104.5%,说明该方法灵敏度高,结果准确可信。利用该方法对10批不同市售海参中5种有毒元素含量进行了测定,结果表明10批市售海参中的AS含量均超标,部分批次海参中的Cd、Pb超标,说明市售海参确实存在有毒元素超标的问题。A method based on microwave sample digestion and inductively coupled plasma-mass spectrometry(ICP—MS) detection was established for determination of Cu,As,Cd,Hg and Pb in holothurian sold on the market.Indium was used as internal standard element to calibrate the results of determination of these five elements.Determination limits of these five elements were in the range of 0.006～0.063 μg/g,coefficient of variation(CV%)were in the range of 0.2%～4.0%,and the recovery of In was in the range of 96.7%～104.5%.The method has been applied to determine the five poisonous elements in ten holothurian samples sold on the market.The results indicated that the over-standard problem of poisonous elements in holothurian sold on the market existed.The content of total arsenic was over standard in all the ten samples,but the over standard problem of lead and cadmium was only found in part of the samples.国家自然科学基金(20675021);中国近海海洋综合调查与评价908专项(908-02-05-04)资

Primary Exploration on Quality Evaluation of Holothurian Sold on the Market by HPLC Fingerprint

以10批不同产地刺参HPlC指纹图谱中的6个共有峰为评价指标,结合相似度分析,对不同刺参质量进行评价和检验。结果表明:不同批次刺参样品的6个色谱峰在指纹图谱分析过程中色谱行为相同,峰面积大,特征性强,能反映刺参的固有化学特征。结合相似度分析,可用于刺参质量评价。An optimized high performance liquid chromatography (HPLC) method was developed for the analysis of holothurian and the HPLC fingerprint was established from 10 batches of the holothurians from different habitats.The HPLC fingerprint showing 6 common characteristic peaks was used to explore the quality evaluation of holothurian and distinguish from the fakes with the similarity analysis.This method is accurate and reliable,providing a scientific basis for the quality control of holothurian and can be used to evaluate the quality of holothurian sold on the market.我国近海海洋综合调查与评价908专项(908-02-05-04):海洋药用生物资源评价和《中华海洋本草》编纂;海洋一所基本科研业务专项(GY-022008T32):海洋绿藻活性成分分析、鉴

Analysis of Alkaloids in Coptis chinensis Franch by High Performance Capillary Electrophoresis-Electrospray Ionization Time of Flight Mass Spectrometry

建立了高效毛细管电泳-电喷雾飞行时间质谱联用(HPCE-ESI-TOF/MS)快速定性分析黄连中生物碱类化合物的分析方法.使用未涂层石英毛细管,以50mmol/L乙酸铵-0.5%甲醇溶液(用氨水调至pH=7.2)作为运行缓冲液,分离电压为25kV;鞘液组成为50%甲醇-49.5%水-0.5%乙酸,鞘液流速为4μL/min;质谱选用正离子模式,碰撞电压(Fragmentor)为100V.结果表明,通过各色谱峰紫外光谱和质谱测得精确分子量结果,结合文献,对黄连中7种生物碱进行了鉴定.表明本方法简便、快速,是黄连中生物碱类化合物快速分离、鉴别的有效方法.A new method for the analysis of alkaloids in Coptis chinensis Franch was established by high performance capillary electrophoresis-electrospray ionization time of flight mass spectrometry (HPCE-ESI-TOF/MS). The real samples were separated by an uncoated capillary. 50 mmol/L ammonium acetate containing 0.5% methanol (pH=7.2) was used as the running buffer, and separation voltage was 25 kV. A coaxial sheath flow interface was used as the CE-MS interface, and a 50% methanol-49.5% water- 0.5% acetic acid mixture was used as the sheath liquid with a flow rate of 4 μL/min. The lens voltages in a positive ion mode with a collision induced dissociation (CID) voltage of 100 V were used for ESI-TOF/MS analysis. Seven alkaloids in Coptis chinensis Franch methanol extracts were separated and identified by CE-DAD and CE-ESI-TOF/MS. The coupling of HPCE separation with accurate mass measurement capability of ESI-TOF/MS provides an attractive tool for the identification of alkaloid compounds in Coptis chinensis Franch.国家自然科学基金重点项目(No.20235020);; 青岛“2004将才计划”(No.04-3-JJ-11);; 共建生物医药研发测试中心(No.LS-05-KJZX-76)资助项目