Extracting from the Past and Reflecting on Current Painting Art ——Contemporary Analysis and Thought on the Classic Mineral Painting of Zhva-lu-dgon-pa Ttemple-monastery in Tibet

岩彩艺术作为近十几年才逐渐在中国凸显出来的绘画形式语言，它适应现代的审美趣味，顺应了时代发展潮流，已令世人注目。然而，鲜为人知的是：岩彩这一当今画坛的新形式却拥有两千多年的丝绸之路多元文化融汇的传统。对岩彩艺术之传统进行深入的分析与探讨，对复兴岩彩这一既有文化传承价值又具有现代审美趣味的绘画形式具有特殊的意义。丝绸之路为我国留下了众多至今都让人为之惊叹的优秀古典岩彩壁画艺术瑰宝，西藏夏鲁寺古典壁画就是其中的一颗璀璨明珠。本文从西藏日喀则地区夏鲁寺古典岩彩壁画艺术的历史文化背景入手，探讨在多元文化因子影响下，夏鲁寺古典岩彩壁画多元并存的艺术风格，形式语言、表现技法，以及其独特的绘制材料；最后，总...Mineral painting as a language of painting gradually develops fast in a distinct way during the last decade. It suits people’s art taste in modern society, conforms to the current development trend, and becomes well known in the whole world. However, remarkably, mineral painting as a very new form of painting in current art field has a tradition over two thousand years, which incorporates multi-cu...学位：文学硕士院系专业：艺术教育学院美术系_美术学学号：2005130154

沿海开放省区内的“俱乐部收敛”——以福建为例

文章以福建为例,通过计量分析,揭示了沿海开放省区内的俱乐部收敛现象:1990到2006年,福建9个设区市急剧地收敛为福厦泉与其他六市两个俱乐部,它不同于传统的闽东南沿海地区和闽西北内陆地区划分。研究指出:向两个俱乐部收敛的主要因素是地区间投资、工业化水平和教育投入上的差异

Intelligent design of hammer mill based on SolidWorks

为适应现代技术发展及产品快速更新的要求,针对机械产品中装配体结构的快速造型设计问题,以SOlIdWOrkS2011为开发平台,运用VC++编程语言,建立了锤片式粉碎机的装配体实体模型,研究了以dll开发方式进行锤片式粉碎机产品智能化与自动化设计的方法,完成了用户界面的开发。以尺寸驱动为特征,参数化驱动实现锤片式粉碎机的重新建模,自动完成了整体模型的变动,并结合实例具体说明程序代码的设计,生成了自主设计的一套锤片式粉碎机参数化建模插件。研究结果表明:该智能化设计系统可以快速、自动修改锤片式粉碎机装配下零件的参数,并且输出形象直观的装配体三维实体模型,从而实现锤片式粉碎机设计的智能化及自动化,加快了产品设计过程,提高了效率。In order to adapt the development of the modern technology and the fast renewal of the product request,aiming at rapid modeling design of the assembly in mechanical product,based on SolidWorks2011 development platform and made use of VC + + programming language embedded in SolidWorks,the solid model of the hammer mill was established,and the intelligent and automatic design method by the DLL of hammer mill was researched,the development of user interface was completed.Characterized by the dimension-driven,the parameter-driven re-modeling of hammer mill was achieved,the whole model was changed automatically,and the procedural codes combined with specific examples were designed.As a result,a parametric modeling plug-in about the self-designed hammer mill was generated.The results show that this intelligent design system can modify the parameters of the fitted parts under hammer mill quickly and automatically,and output intuitive three-dimensional solid model of the assembly,which realizes intellectualization and automation of hammer mill design,speeds up the process of product design and enhances the efficiency

大孔吸附树脂分离纯化龙胆药材中龙胆苦苷和马钱子苷酸的研究

目的:建立利用大孔吸附树脂对龙胆药材中龙胆苦苷和马钱子苷酸进行富集和分离纯化的方法。方法:采用加速溶剂萃取法对龙胆药材中两种有效成分进行高效提取,比较了D301,AB-8,D101,XDA-1四种大孔树脂对龙胆苦苷和马钱子苷酸的吸附性能,最终确定采用D301型大孔对脂对二者进行富集吸附,对其工艺参数进行优化,全程采用高效液相色谱进行目标化合物浓度检测。结果:化优后的工艺参数为:上样浓度:0.2 g/mL,最大上样量:0.25 g龙胆药材/g树脂,最佳静态吸附时间:8 h,采用8%和55%的乙醇溶液对龙胆苦苷和马钱子苷酸分别进行洗脱;龙胆苦苷和马钱子苷酸分别富集在8%和55%的乙醇洗脱液中,洗脱液浓缩后冷冻干燥,可得到纯度分别为74.3%和80.9%的粗产物,龙胆苦苷和马钱子苷酸的回收率分别为70.11%和67.82%。结论:此法效率较高,操作简便,即可用于实验室制备少量的难以购置的标准品,也可进行放大研究,用于工业生产。国家自然科学基金重点项目(20235020);; 青岛“2004将才计划”(04-3-JJ-11);; 共建生物医药研发测试中心(LS-05-KJZX-76)资

Content of gentiopicroside and loganic acid in Radix gentianae and their fingerprints

To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints.The samples were extracted using ASE for 10 min under 100 ℃ and 9.65 MPa,and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method respectively.Based on this method,the HPLC fingerprints of Radix gentianae were established.Comparing the spectrogram and mass spectrum of the chromatogram peak with the reference value,three compounds in water phase were identified as gentiopicroside,asafetida acid and loganic acid.There is no report of the compounds in chloroform phase.The content of gentiopicroside and loganic acid in samples of different groups were determined,separately.The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint.The water phase fingerprint congruence coefficients of samples from six different areas were above 0.90,however,the chloroform phase fingerprint congruence coefficients were within 0.62-0.99.This method can be used for determination of potent component in Radix gentianae and its quality control.Radix gentianae from different producing areas have the largest diversities,and the diversities embodied in the content of chloroform phase compounds.青岛“2004将才计划”(04-3-JJ-11);; 国家海洋局青年基金资助项目(2005602);; 崂山区政府区校共建生物医药研发测试中心资助(LS-05-KJZX-76)

Determination of Gentiopicroside and Loganic Acid in Radix Gentianae by MEKC and MEEKC Mode

目的建立胶束电动毛细管色谱(MEKC)和微乳液毛细管电动色谱(MEEKC)分析龙胆药材中龙胆苦苷和马钱子苷酸含量的方法。方法采用加速溶剂萃取法(ASE)对龙胆药材进行提取,萃取温度:100℃,压力:9.65MPa,萃取时间:10min。采用未涂层熔融石英毛细管(内径75μm,有效长度50cm)。分别考察了两种分离模式下电泳介质的构成和电泳过程中的各操作参数对样品分离过程的影响,优化了MEKC和MEEKC的分析条件,在各自对应的缓冲液体系下,MEKC和MEEKC分离电压分别为30和22kV,柱温均为25℃,检测波长均为238nm。结果在选定的工作条件下,龙胆苦苷和马钱子苷酸与其他组分达到了基线分离,两种成分的浓度与其响应信号值之间具有较好的线性相关性,加标回收率在96.3%～105.1%之间,检测限均低于10mg·L-1,对6处不同产地的龙胆药材进行了分析,并对测定结果进行了t检验,结果表明,两种模式下,测定结果之间不存在显著性差异,而不同产地的龙胆药材的龙胆苦苷和马钱子苷酸含量之间存在较大差异。结论本方法简便,准确,快速,重现性较好,可用于龙胆药材有效成分的含量测定和质量控制。OBJECTIVE To develop MEKC and MEEKC modes for the determination of gentiopicroside and loganic acid in extracts of Radix Gentianae. METHODS The analyte was extracted from Radix Gentianae samples by accelerated solvent extraction, and the extraction conditions were optimized. Separation and determination were carried out on a bared fused silica capillary(50 cm×75 μm) with corresponding buffer. The run voltage of MEKC and MEEKC were 30kV and 22 kV respectively. Detection wavelength of DAD was at 238 nm and column temperature was 25 ℃. RESULTS The developed ASE-HPCE method was simple and reliable for the determination of gentiopicroside and loganic acid in Radix Gentianae samples with a broad linear dynamic range, a recovery range of 96.3%～105.16%,and the detection limit was below 10 mg·L-1. The contents of gentiopicroside and loganic acid in six samples from different regions were determined by the developed method in two modes. T-test value indicated that the determined contents of gentiopicroside and loganic acid by MEKC and MEEKC were consistent. CONCLUSION The method is simple,accurate,rapid and with good reproducibility.It can be used to determine active components in Radix Gentianae.国家自然科学基金重点项目(20235020);; 青岛“2004将才计划”(04-3-JJ-11);; 崂山区校区共建生物医药研发测试中心资助(LS-05-KJZX-76

高致病性禽流感病毒广谱嵌合抗体的构建表达及活性研究

高致病性禽流感病毒高度变异且缺乏有效的治疗药物.在前期研究工作中,本课题组发现一株鼠源广谱中和单抗13D4,在动物实验中显示出对H5N1禽流感病毒具有广谱治疗效果.在本研究中,从分泌13D4单克隆抗体的杂交瘤细胞株中抽提总RNA,经RT-PCR扩增出轻重链可变区DNA序列,并分别与人IgG1的轻重链恒定区基因序列拼接,构建人-鼠嵌合抗体.筛选出稳定表达嵌合抗体的CHO细胞株,从培养上清中纯化出嵌合抗体.竞争Elisa结果表明,嵌合抗体与鼠源单抗能够识别同一个抗原表位并具有相似的亲和力.血凝抑制反应和中和活性测定结果证明,13D4嵌合抗体保留了对不同亚型H5N1病毒的广谱反应性,并且对两株H5N1病毒具有中和活性.本研究获得的13D4嵌合抗体将具有潜在的治疗价值

集团企业内部资本市场形成动因和运行机制研究

研究了集团企业多元化的发展历程,基于对组织能力的认识,强调了内部资本市场在价值管理中的作用,指出公司层战略在追求母合优势过程中要借助内部资本市场才能发挥作用,结合集团企业的内外部管理环境建立了一个整合的治理框架。The research first focused on the deve-lopment of conglomerate comqany diversification, then based on the realization of organization capability, we emphasized the function of internal capital market on the value management, then pointed out that corporate level strategic for building the parenting advantage need internal capital market. Finally, considering the cong-lomerate’s internal and external environment , this paper provided an integrate governance frame

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近１０年来，中国科学院文献情报系统在嵌入科研一线的知识服务探索中积累大量经验，逐步形成文献资源保障能力分析和咨询、信息素质培训与能力建设、学科专题信息服务、学科情报和战略情报研究、专业文献信息与知识环境建设（专题信息平台建设）、科研机构成果与知识管理等为核心的专业化知识服务模式，并进一步探索嵌入科研过程的文献情报人员、科研人员、科研管理人员、文献情报服务流程与科研工作流程相互协同与协调的机制。Ｉｎｒｅｃｅｎｔｔｅｎｙｅａｒｓ，ｌｉｂｒａｒｉｅｓｏｆＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓｈａｖｅａｃｃｕｍｕｌａｔｅｄｌｏｔｓｏｆｅｘｐｅｒｉｅｎｃｅｓｏｎｈｏｗｔｏｅｘｐａｎｄｔｈｅｋｎｏｗｌｅｄｇｅｓｅｒｖｉｃｅｓｏｆｔｈｅｌｉｂｒａｒｉｅｓｉｎｔｏｔｈｅｓｃｉｅｎｔｉｆｉｃｒｅｓｅａｒｃｈｉｎｆｏｒｍａｔｉｏｎｅｎｖｉｒｏｎｍｅｎｔ，ａｎｄｈａｖｅｇｒａｄｕａｌｌｙｅｓｔａｂｌｉｓｈｅｄｔｈｅｓｐｅｃｉａｌｉｚｅｄｍｏｄｅｏｆｋｎｏｗｌｅｄｇｅｓｅｒｖｉｃｅｓｆｏｒｔｈｅｉｎｓｔｉｔｕｔｅｓｏｆＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓ，ｓｕｃｈａｓａｎａｌｙｚｉｎｇｔｈｅｓｕｐｐｏｒｔｃａｐａｂｉｌｉｔｉｅｓｏｆｓｃｉｅｎｔｉｆｉｃｌｉｔｅｒａｔｕｒｅａｎｄｉｎｆｏｒｍａｔｉｏｎ，ｔｒａｉｎｉｎｇｉｎｆｏｒｍａｔｉｏｎｌｉｔｅｒａｃｙｏｆｓｃｉｅｎｔｉｓｔｓ，ｓｔｕｄｅｎｔｓａｎｄｓｔａｆｆｓ，ｐｒｏｖｉｄｉｎｇｓｃｉｅｎｔｉｆｉｃｉｎｆｏｒｍａｔｉｏｎｓｅｒｖｉｃｅｏｆｓｃｉｅｎｔｉｆｉｃｓｕｂｊｅｃｔｓａｎｄｔｏｐｉｃｓ，ａｎａｌｙｚｉｎｇｓｕｂｊｅｃｔｉｎｆｏｒｍａｔｉｏｎａｎｄｓｔｒａｔｅｇｉｃｉｎｆｏｒｍａｔｉｏｎ，ｄｅｖｅｌｏｐｉｎｇｓｕｂｊｅｃｔｋｎｏｗｌｅｄｇｅｅｎｖｉｒｏｎｍｅｎｔａｎｄｉｎｆｏｒｍａｔｉｏｎｐｌａｔｆｏｒｍ，ａｎｄｍａｎａｇｉｎｇｒｅｓｅａｒｃｈａｃｈｉｅｖｅｍｅｎｔｓａｎｄｋｎｏｗｌｅｄｇｅａｓｓｅｔｓｏｆｉｎｓｔｉｔｕｔｉｏｎｓ．Ｆｕｒｔｈｅｒ，ｍａｎｙｉｎｓｔｉｔｕｔｉｏｎａｌｌｉｂｒａｒｉｅｓｈａｖｅｔｒｉｅｄｔｈｅｉｒｂｅｓｔｔｏｅｓｔａｂｌｉｓｈｔｈｅｓｅｒｖｉｃｅｍｅｃｈａｎｉｓｍｉｍｂｅｄｄｅｄｉｎｔｏｔｈｅｓｃｉｅｎｔｉｆｉｃｒｅｓｅａｒｃｈｐｒｏｃｅｓｓｅｓｗｈｉｃｈｉｎｃｌｕｄｅｓｔｈｅｌｉｂｒａｒｉａｎｓａｎｄｒｅｓｅａｒｃｈｅｒｓａｎｄｍａｎａｇｅｒｓ，ａｓｗｅｌｌａｓｔｈｅｃｏｌｌａｂｏｒａｔｉｏｎｓｏｆｉｎｆｏｒｍａｔｉｏｎｓｅｒｖｉｃｅｆｌｏｗｓａｎｄｔｈｅｓｃｉｅｎｔｉｆｉｃｒｅｓｅａｒｃｈｐｒｏｃｅｓｓ