Diatom diet selectivity by early post-larval abalone Haliotis diversicolor supertexta under hatchery conditions

Benthic diatoms constitute the primary diet of abalone during their early stages of development.To evaluate the dietary preferences of early post-larval abalone,Haliotis diversicolor supertexta,we analyzed the gut contents of post-larvae that settled on diatom films.We compared the abundance and species diversity of diatom assemblages in the gut to those of the epiphytic diatom assemblages on the attachment films,and identified 40 benthic diatom species in the gut contents of post-larvae 12 to 24 d after settlement.The most abundant taxa in the gut contents were Navicula spp.,Amphora copulate,and Amphora coffeaeformis.Navicula spp.accounted for 64.0% of the cell density.In the attachment films,we identified 110 diatom species belonging to 38 genera.Pennate diatoms were the dominant members including the species Amphiprora alata,Cocconeis placentula var.euglypta,Cylindrotheca closterium,Navicula sp.2,and A.coffeaeformis.Nano-diatoms(<20 μm in length) accounted for a considerable proportion of the total species..

泥鳅对创伤急性反应的功能蛋白质组学研究

研究背景：几乎所有动物受到创伤和感染等外界伤害时都会启动急性反应系统（acutephaseresponse,ARP）进行快速的自我保护，包括抵御微生物的入侵，促进创伤的修复，帮助机体恢复正常静息状态等，其中涉及众多蛋白质（APP）水平的变化。APR的研究在哺乳动物中有较多的报道，研究成果多涉及人类医疗领域。低等生物在更大程度上倚赖APR的保护作用，例如鱼类就是一种很好的APR研究模型。蛋白质组学的发展为APR的研究带来了快速，高灵敏度，高分辨率，高通量的研究方法，其从整体的角度研究APR体系并可以快速鉴定候选APP的能力是其它方法所不具备的。 研究目的：研究泥鳅创伤后血清和皮肤蛋白质组的变化...Background: Innate immunity system provide the body with its first line of protection against external injury and such a rapid, non-specific mechanism can almost be found all through the living kingdom. Both cells and secreted molecules of innate immunity are dynamic in regard to their readiness status and the process of cell activation during the adaptive alteration of cell physiology at the init...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：19992603

甲壳动物基因研究进展

国家海洋局第三海洋研究所海洋工程重点实验室开放课题基金资助项

p62 antigenic analysis with IgG class of autoantibody from patients with cancers

目的 :研究p6 2不同区域的抗原性。方法 :取p6 2全长重组子及克隆其不同区域的 3个重组子进行表达和纯化。以各重组蛋白为包被抗原 ,建立检测IgG类抗p6 2自身抗体的ELISA技术。结果 :恶性肿瘤患者血清中抗p6 2各片段自身抗体阳性率以抗p6 2 1为最高 ,抗p6 2 3次之 ,抗p6 2 2最低 ,3者均低于抗全长p6 2。抗全长p6 2的阳性率与抗p6 2 1的阳性率无明显差异 ,而与抗p6 2 2和抗p6 2 3均有非常显著差异。除肺癌外 ,肝癌、胃癌、肠癌、食管癌和乳腺癌患者的 3个抗原片段的自身抗体的叠加阳性率均低于全长p6 2。结论 :p6 2的抗原性主要位于p6 2 1,但其结构的完整性对维持其抗原性具有重要意义。Objective:To carry out p62 antigenic analysis with IgG class of autoantibody from patients with cancers.Methods:Three fragments of p62 gene, named as p62 1, p62 2 and p62 3, were cloned using cloned p62 as template Purified recombinant p62, p62 1, p62 2 and p62 3 were used as coating antigen for establishment of ELISA to detect anti p62 of class of IgG Results:The positive rates of autoantibody to p62 fragments were anti p62 1 > anti p62 3 > anti p62 2 in patients with cancers All of them were lower than that of autoantibody against full length p62 The frequency of autoantibody to full length p62 was significantly higher than that of anti p62 2 and anti p62 3, but no difference with anti p62 1 Totally positive rates of autoantibody against the three fragments were lower than those of full length p62 in patients with hepatocellular cancer, gastric cancer, colorectal cancer, esophagus cancer and breast cancer but with lung cancer Conclusion:Antigenic sites in p62 were mainly located in p62 1 and the integrity of p62 structure is important for antigenic expression教育部优秀青年骨干教师资助课题和国家自然科学基金资助课题( 3 0 0 80 0 2 9

Curcusone C诱导卵巢癌SK-OV-3细胞凋亡

近年来麻风树(Jatropha curcas)的生物活性研究如火如荼,但其二萜类化合物的抗癌活性及分子机制研究仍知之甚少.通过萃取和柱层析方法从麻风树的根皮中分离提取到一种二萜类化合物Curcusone C,探讨了该化合物对卵巢癌SK-OV-3细胞增殖、细胞凋亡的影响.实验结果表明,Curcusone C能显著抑制SK-OV-3细胞的增殖,且随着药物浓度的增加其抑制作用呈明显的剂量依赖效应,其48 h半数抑制浓度IC50为160 nmol/L;同时,Curcusone C能显著诱导SK-OV-3细胞凋亡,DAPI染色结合显微镜观察发现400 nmol/L Curcusone C处理后的细胞呈现..

Issues and Countermeasures in the Implementation of "Medical Cloud Project" of Nanjing Military Area

目的:探讨“医云工程“实施中可能存在的问题,并提出相应对策。方法:对医院在“医云工程“实施过程中遇见的相关问题进行分析、总结。结果:云计算平台是破解当前医院数字化建设面临瓶颈问题的有效途径。结论:云计算是安全、可靠的,符合医院信息化建设发展趋势,是引领医院信息化建设走向深入的必经之路。Objective:Discuss the issues of hospital cloud computing engineer which may exist in the implementation of hospital and put forward the corresponding countermeasures.Methods:To analyze and summarize the related problems in the process of hospital cloud computing engineer implementation in our hospital.Results:The cloud computing platform is an effective way to resolve the Bottlenecks of hospital digital construction.Conclusion:Cloud computing is safe,reliable,and compliance with hospital information construction trends,which is the only way to lead the information construction of hospital in-depth.南京军区卫勤专项课题-基于云计算的医院信息技术平台的构建与研究(编号:12WQZ04)~

Information Security Management Mode Research and Practice of Medical Cloud Project of Nanjing Military Region

目的:针对军区医院“医云工程“的安全现状,探讨符合医院信息安全管理的新模式。方法:通过分析、比较传统与云平台不同环境下安全的差异性特点,总结医院云安全管理面临的新挑战。结果:建立以软件定义为主、硬件为辅的信息安全管理新模式,确保“医云工程“的信息安全。结论:建立符合云计算平台的安全管理模式是确保“医云工程“平稳落地的重要保证。Objective:A new mode of hospital information security management is explored according to the security status of Nanjing military region hospital cloud computing engineering.Methods:New challenges of hospital cloud computing security management are summarized after the analysis and comparison of security difference between traditional and cloud platform environment.Results: A new mode of hospital information security management is established which is defined based on software supplement more than hardware to make sure the information security of " Hospital Cloud Computing Engineering ".Conclusion:It is important to establish reasonable security management modes of the cloud computing platform that may ensure the smooth implementation of hospital cloud computing engineering.南京军区卫勤专项课题-基于云计算的医院信息技术平台的构建与研究(编号:12WQZ04)~