HYDROCHEMICAL COMPONENT AND ITS DYNAMIC TRENDS OF GROUNDWATER IN THE PLAIN OF SANTUN RIVER BASIN

该文依据水文地质条件将三屯河流域平原区划分为单一结构潜水子系统和多层结构潜水 -浅层承压水子系统及深层承压水子系统。通过地下水水质简分析数据 ,分析了各地下水子系统中主要离子 ,TDS和水化学类型的特征及其变化 ,并初步讨论了地下水系统的水循环特点In accordance with hydrogeological condition, three groundwater systems are presented in the paper, they are the phreatic water systems with single-layer structure, phreatic water together with shallow confined groundwater system and deep confined groundwater with multi-layer structures in the plain of Santun river basin The component and distribution of the major ions, TDS and hydrochemical types and their variations of the above-mentioned groundwater systems are analyzed, meanwhile, the circulatory characteristics of groundwater system are discussed preliminarily九五”国家重点科技攻关项目 (96 - 91 2 - 0 1 - 0 3S

Construction of eukaryotic vector pcDNA3.1-flag-pygo2 and expression in C6 cell

目的构建pcDNA3.1-flag-pygo2真核表达载体并在C6细胞中进行表达。方法从小鼠脑胶质细胞中提取总RNA,RT-PCR法反转录合成c DNA,设计引物,调取目的片段,与pcDNA3.1-flag载体连接后转化大肠杆菌DH5α,LB平板筛选菌落,提取质粒。重组质粒pcDNA 3.1-flag-pygo2经过酶切鉴定及测序后,阳离子脂质体法转染C6细胞并经免疫细胞化学染色及蛋白印迹检测重组体的表达。结果重构质粒pcDNA 3.1-flag-pygo2经限制性核酸内切酶EcoRⅠ,HindⅢ酶切分析及测序检查,表明真核表达载体构建正确;瞬时转染C6细胞后,免疫细胞荧光染色及蛋白印迹检测表明转染细胞能够表达外源Pygo2基因。结论成功构建了pcDNA3.1-flag-pygo2真核表达载体并能够在真核细胞中进行表达,这为今后研究pygo2基因在胶质瘤中的作用机制奠定了基础。Objective To construct the eukaryotic expression vector pcDNA3.1-flag-pygo2 and express the combined protein in C6 cell line.Methods To extract total RNA from primary glial cell of mouse and to synthesize c DNA by RT-PCR,then design primer and clone whole segment of pygo2 gene.After the targeted gene was inserted into vector pcDNA3.1-flag,the recombined plamid was transformed into E coli DH5α for LB agar plate screening and the recombined plasmid were extracted and purified.After verification by double enzyme digestion and sequencing.,the constructed eukaryotic expression plamid was transfected to C6 cell line by lipofectamin method,finally the protein expression was detected by immunocytochemical staining as well as western blot.Results The new constructed vector was confirmed by restricted enzyme Eco R I,HindIII digestion assay and correct Pygo2 was verified by sequenceing.finally,pcDNA3.1-flag-hpygo2 can express exogenous pygo2 gene in glioma C6 cell line after transient transfection by the determination of immunocytofluorescent staining and western blot.Conclusion The new plamid pcDNA3.1-flag-pygo2 was constructed successfully,and can express fused protein in eukaryotic cell,which establish the foundation for future research on pygo2 gene function in human glioma

pcDNA3.1-HIF-1α重组质粒的构建及其初步鉴定

目的探讨HIf-1α与前列腺癌发病机制之间相关性提供研究工具。方法采用TrIzOl法裂解细胞,提取HIf-1α且以该rnA为模板逆转录为CdnA,然后行PCr处理扩增HIf-1α基因功能片段区域,克隆至真核表达载体PCdnA3.1上,转化至大肠杆菌dH-5α,小提质粒,酶切凝胶电泳鉴定,序列测定,经鉴定为正确序列后中抽质粒,采用fugEnE试剂转染至前列腺癌细胞PC-3,经g418筛选,建立稳定表达HIf-1α的前列腺癌细胞系PCdnA3.1-HIf-1α-PC-3,采用WESTErn印迹鉴定重组质粒的表达。结果 PCr扩增基因片段大小为2.89 kb,克隆至真核载体PCdnA3.1,酶切凝胶电泳可见两个条带,大小大约为5.3 kb和2.89 kb,与预期的大小相符合,序列测定与gEnbAnk公布的一致,WESTErn印迹验证其在细胞内能表达。结论重组质粒PCdnA3.1-HIf-1α能够在前列腺癌PC-3表达,构建成功,为研究HIf-1α与前列腺癌提供了一个工具。厦门市科技局科研基金资助(No.3502Z20084002

ISOTOPE COMPONENT CHARACTERISTICS OF GROUNDWATER IN TIANSHAN NORTH PIEDMONT PLAIN AND ITS SIGNIFICANCE ──TAKING SANTUN RIVER BASIN PLAIN AS AN EXAMPLE

该文依据水文地质条件将三屯河流域平原区划分为单一结构潜水子系统、多层结构潜水—浅层承压水子系统和深层承压水子系统。通过氢氧稳定同位素、氚同位素和碳 - 14的测试数据 ,分析了各地下水子系统的环境同位素组成 ,并初步讨论了地下水系统的水循环特点。 【英文摘要】 In the paper, In accordance with hydrogeological conditions,groundwater of Santun River basin plain is divided into phreatic water subsystem with single-layer structure,phreatic water both of shallow confined groundwater subsystem and deep confined groundwater subsystem with multi-layer structures.By means of the tested data of Hydrogen-Oxygen stable isotope,and isotopes of tritium and Carbon-14,the environmental isotope components of various groundwater subsystems being analyzed,meanwhile,the circulatory c...“九五”国家重点科技攻关项目 (96 -912 - 0 1- 0 3S

Study on the mechanism of infiltration and evaporation of phreatic groundwater in the planting conditions

在天山北麓昌吉地下水均衡试验场 ,选择具有代表性的作物玉米进行不同埋深条件下的模拟种植试验 ,分析研究种植条件下不同潜水埋深水平的潜水入渗补给量、潜水蒸发损耗量、土壤水储存量和作物耗水量的变化规律。研究成果对于地下水资源和土壤水资源评价以及地下水和土壤水资源的有效调控利用具有重要实用意义In the text station of the groundwater balance, located in the northern slope of Tianshan Mountain, the corn, local typical crop, is chosen to plant for the text under the conditions of the different groundwater level depth. Through the text, the infiltration and evaporation of the phreatic groundwater, the storage of the soil moisture, and the water requirement of crop are observed and analyzed. The experimentation achievements are significant for the groundwater resource evaluation, the soil moisture resources evaluation, as well as controlling and utilizing effectively the groundwater and soil moisture.“九五”国家重点科技攻关项目的部分研究成果 ( 96 912 0 1 0 3S

Prediction of Energy Resolution in the JUNO Experiment

International audienceThis paper presents the energy resolution study in the JUNO experiment, incorporating the latest knowledge acquired during the detector construction phase. The determination of neutrino mass ordering in JUNO requires an exceptional energy resolution better than 3% at 1 MeV. To achieve this ambitious goal, significant efforts have been undertaken in the design and production of the key components of the JUNO detector. Various factors affecting the detection of inverse beta decay signals have an impact on the energy resolution, extending beyond the statistical fluctuations of the detected number of photons, such as the properties of liquid scintillator, performance of photomultiplier tubes, and the energy reconstruction algorithm. To account for these effects, a full JUNO simulation and reconstruction approach is employed. This enables the modeling of all relevant effects and the evaluation of associated inputs to accurately estimate the energy resolution. The study reveals an energy resolution of 2.95% at 1 MeV. Furthermore, the study assesses the contribution of major effects to the overall energy resolution budget. This analysis serves as a reference for interpreting future measurements of energy resolution during JUNO data taking. Moreover, it provides a guideline in comprehending the energy resolution characteristics of liquid scintillator-based detectors

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies