Ethical Reflection on Standardized Management of Intestinal Microbiota Transplantation

肠道微生态系统是人体健康所必需的重要且复杂的生物系统。肠道微生态系统的紊乱参与人体多种疾病的发生发展过程。而肠菌移植技术可有效调节肠道菌群，修复失衡的肠道微生态，为当今多种胃肠道及胃肠以外疾病的治疗带来新的突破。然而，目前尚缺乏完整的、系统性的肠菌移植技术管理规范。就肠菌移植技术规范化管理所涉及的内容进行探讨，并从医学伦理学角度思考规范化管理中所涉及的伦理问题，以期推动肠菌移植技术的临床应用。Intestinal microecology is an important and complex biological system necessary for human health. Its disorder is involved in the development of various diseases of human body. The technology of intestinal microbio- ta transplantation can effectively regulate the intestinal flora, repair the imbalance of the intestinal microecology, and bring a new breakthrough for the treatment of many diseases of gastrointestinal tract and outside gastrointestinal tract. However, there is still no systematic and complete management standard for intestinal microbiota transplanta- tion technology. This paper discussed related content involved in standardized management of intestinal microbiota transplantation technology and reflected the ethical problems involved in standardized management from the perspec- tive of medical ethics, in order to promote the clinical application of intestinal microbiota transplantation technology

一种蛋白酶抑制剂编码基因及其表达与应用

本发明涉及基因工程技术领域，具体涉及一种来源于菊芋的蛋白酶抑制剂编码基因(HtCPI)及其表达和应用。HtCPI基因碱基序列如SEQ?ID?NO：1所示；或，与SEQ?ID?NO：1所示碱基序列同源性在90%以上的基因。本发明提供的HtCPI基因及其表达载体能用于植物遗传转化，提高植物抗逆性。另外本发明提供的HtCPI基因能够用于体外表达，在生物源杀虫剂生产方面具有重要应用价值

一种菊芋抗虫基因及其表达载体构建方法和应用

本发明涉及基因工程技术领域，具体涉及一种菊芋抗虫基因及其植物表达载体构建方法和应用。菊芋抗虫(JaSPI)基因碱基序列如SEQ?ID?NO：1所示；或，与SEQ?ID?NO：1所示碱基序列同源性在85%以上的基因。JaSPI编码的氨基酸序列如SEQ?ID?NO：2所示。本发明提供的JaSPI基因及其表达载体能用于植物遗传转化，提高植物抗虫性及抗逆性。另外本发明提供的JaSPI基因能够用于体外表达，为获得新的生物杀虫剂、辅助Bt等生物制剂共同预防农业害虫提供了新的途径

Artichoke insect resistance gene as well as expression vector construction method and application thereof

本发明涉及基因工程技术领域，具体涉及一种菊芋抗虫基因及其植物表达载体构建方法和应用。菊芋抗虫(JaSPI)基因碱基序列如SEQ ID NO：1所示；或，与SEQ ID NO：1所示碱基序列同源性在85％以上的基因。JaSPI编码的氨基酸序列如SEQ ID NO：2所示。本发明提供的JaSPI基因及其表达载体能用于植物遗传转化，提高植物抗虫性及抗逆性。另外本发明提供的JaSPI基因能够用于体外表达，为获得新的生物杀虫剂、辅助Bt等生物制剂共同预防农业害虫提供了新的途径

一种菊芋组培繁殖方法

一种菊芋组培繁殖方法，包括如下步骤：对外植体进行消毒处理；将外植体接种至愈伤组织诱导培养基，在23℃-25℃下，暗培养至出现愈伤组织；取愈伤组织，将其切成小块，转接至不定芽诱导培养基中，光照培养至长出不定芽；将不定芽转移至继代培养基中，在23℃-25℃下，光照培养20-25天；将继代苗转入生根培养基中，在23℃-25℃下，光照培养15-20天；取出生根的组培苗，洗去根部培养基，栽入基质中，再移栽至大田。上述菊芋组培繁殖方法，可以采用菊芋不同部位作为外植体进行组培繁育，所使用的外植体容易取得，实验操作简单易行。且通过通用的MS培养基搭配不同生长激素得到了适宜菊芋组织培养的专用培养基，能有效地进行菊芋的组织培养，使菊芋得到快速繁殖

Prediction of Energy Resolution in the JUNO Experiment

International audienceThis paper presents the energy resolution study in the JUNO experiment, incorporating the latest knowledge acquired during the detector construction phase. The determination of neutrino mass ordering in JUNO requires an exceptional energy resolution better than 3% at 1 MeV. To achieve this ambitious goal, significant efforts have been undertaken in the design and production of the key components of the JUNO detector. Various factors affecting the detection of inverse beta decay signals have an impact on the energy resolution, extending beyond the statistical fluctuations of the detected number of photons, such as the properties of liquid scintillator, performance of photomultiplier tubes, and the energy reconstruction algorithm. To account for these effects, a full JUNO simulation and reconstruction approach is employed. This enables the modeling of all relevant effects and the evaluation of associated inputs to accurately estimate the energy resolution. The study reveals an energy resolution of 2.95% at 1 MeV. Furthermore, the study assesses the contribution of major effects to the overall energy resolution budget. This analysis serves as a reference for interpreting future measurements of energy resolution during JUNO data taking. Moreover, it provides a guideline in comprehending the energy resolution characteristics of liquid scintillator-based detectors

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies