The Research of White Spot Syndrome Virus Nonstructural Protein ICP11

白斑综合征病毒(WSSV)是一种对虾养殖业中危险极大的传染性病原体,它是一种双链环状dnA杆状型病毒.ICP11是WSSV感染宿主后产生的一种高丰度表达蛋白,根据ICP11基因的序列,通过PCr扩增获得了该全长基因,将其克隆在载体PET-HIS上,以E.COlIbl21为宿主菌,成功表达并纯化了含HIS标记的目的蛋白,并制备了特异性鼠抗血清.WESTErn blOT杂交实验结果显示,该蛋白不存在于纯化的病毒粒子的结构蛋白中,只存在于病毒感染的虾中肠组织的总蛋白中.这说明ICP11是WSSV的一种非结构蛋白.通过fAr-WESTErn blOT杂交分析,发现重组表达的ICP11会发生自身蛋白的聚合作用.凝胶过滤色谱层析法进一步证实ICP11蛋白可以聚合为二聚体.White spot syndrome virus(WSSV),a rod-shaped double-stranded circular DNA virus,is a kind of major dangerous infectious pathogen in shrimp culture industry.ICP11(Infected cell protein 11)was generated from a high abundance protein in WSSV-infected host.According to icp11 gene sequence,the full-length gene was amplified by PCR and then cloned in vector pET-His.The gene with 6 His-tag was expressed and purified,then was used to prepare specific antibody.Western blot results revealed that the protein is not present in the structure proteins of purified virions,but only in WSSV-infected shrimp total protein extracted from midgut tissue.It is suggested that ICP11 is a WSSV nonstructural protein.ICP11 was found being capable of self-interaction through Far-western blot analysis,and capable of self-multimerization for a dimer using the method of gel filtration chromatography.国家自然科学基金(40776096)资

体操运动员比赛时尿内儿茶酚胺的排出量

测量了37名体操运动员在比赛前和训练前的血压和脉率,并测了25名运动员赛前、赛后和训练后尿内儿茶酚胺的排出量。结果发现男子组赛前舒张压比训练前为高。比赛后尿内去甲肾上腺素排出量男女组均显著高于比赛前,男子组训练后尿内去甲肾上腺素排出量比比赛后略低,而女子组则比比赛后有明显降低。说明男子组去甲肾上腺素的排出增加与情绪的关系较少,而女子组则与情绪关系较密切。赛后肾上腺素排出量男女组均有明显增加,训练后排出量均比比赛后排出量低。女子组在赛前、赛后、训练后肾上腺素排出量均比男子组为低,但差异未达显著水平。比赛中发挥好的肾上腺素排出量倾向较低。</p

Microstructural and Interfacial Characterization of Ti-V Diffusion Bonding Zones

Ti and V were bonded together and subjected to high-temperature treatment at 1000 or 1100 degrees C for 16 h to study the microstructural evolution and interfacial behavior of Ti-V diffusion interfaces. The samples were prepared by electro-polishing and analyzed using scanning electron microscopy, electron probe microanalysis, electron back-scattered diffraction, and nano-indentation. The results indicated that Ti-V diffusion bonding interfaces comprises a martensite Ti zone, a body-center-cubic Ti (beta-Ti) zone, and a V-based alloy zone. They are divided by two composition interfaces with V contents of similar to 13.5% and similar to 46%. The original interface between the pure Ti and the V alloy substrate falls within the beta-Ti zone. The observation of acicular-martensite rather than lath-martensite is due to the distortion caused by the beta-to-alpha phase transformation in the adjacent pure Ti. The recrystallization of beta-Ti is distributed along the interface direction. The hardness varies across the Ti-V interface bonding zones with the maximum value of 7.9 GPa

体操运动员应激反应特点的研究

情绪紧张或应激状态可引起一系列生理生化反应。但是后天的训练和应激经验能否影响这些反应尚所知不多。本实验比较了运动员与非运动员在实验室内完成一复杂辨别反应时,即在与运动无关的应激状态下尿内儿茶酚胺的分泌量、心率、心律、呼吸率和呼吸积分等变化的异同。发现在紧张性作业时运动员尿内几茶酚胺的分泌仅有小量的不显著的增加,而非运动员则有显著增加。运动员和非运动员尿内儿茶酚胺分泌量差别显著。在紧张性作业时运动员和非运动员的心率均有显著增加,R&mdash;R间期标准差则均变小。运动员的心率在休息或作业时均比非运动员慢,R&mdash;R间期标准差则均大于相应条件下非运动员的。紧张性作业时运动员和非运动员呼吸率均明显增加,作为相对呼吸流量的呼吸积分值均显著下降。运动员的呼吸积分值均低于相应条件下非运动员的,但两组间差异未达显著水平。结果表明后天的训练和应激经验对机体在应激状态下尿内几茶酚胺分泌量、心率、心律、相对呼吸流量等生理反应有不同程度的影响。</p

产量与经济效益共赢的高效生态农业模式:以弘毅生态农场为例

化学物质的大量投入以及元素不能循环导致农田生态系统退化,耕地质量和产量均呈下降趋势,食物链受到污染.本研究从低产田开始,通过秸秆养牛、腐熟牛粪还田恢复地力;以物理+生物方法控制虫害;以人工+机械管理杂草,停用农药、化肥和除草剂,同时不用地膜、人工合成激素、转基因种子生产优质安全食品,并在线上与线下销售.10年的长期实验结果表明,所在村庄农田生态环境改善,减少农药用量58.3%;物理+生物控虫效果明显,每盏灯年捕获量从2009年的33 kg下降到2014年的2.1 kg,下降93.8%;年消耗秸秆1000 t,秸秆利用率从1.1%提高到62.5%.有机肥还田提高了土壤生物多样性,有机果园蚯蚓数量317条m~(-2),而普通果园只有16条m~(-2);大量有机肥还田(75 t hm~(-2)),土壤有机质从实验初期的0.7%提高到2.4%.粮食产量从最初的11.43 t hm~(-2)提高到目前的17.43 t hm~(-2),其中冬小麦(Triticum aestivum)、夏玉米(Zea mays)、大豆(Glycine max(Linn.)Merr.)和花生(Arachis hypogaea Linn.)产量分别超出山东省平均水平42.6%,60.9%,32.2%和38.1%.由于质量好,产品已销售往除西藏以外的30个省、市、自治区,经济效益明显,平均每公顷效益是普通农田的3~5倍,带动所在村庄67户农民从事高效生态农业.本研究可为国家制定生态农业发展规划、精准扶贫、农村环境保护等提供科学依据

Aripiprazole versus other atypical antipsychotics for schizophrenia

BACKGROUND: In most western industrialised countries, second generation (atypical) antipsychotics are recommended as first line drug treatments for people with schizophrenia. In this review we specifically examine how the efficacy and tolerability of one such agent - aripiprazole - differs from that of other comparable second generation antipsychotics. OBJECTIVES: To evaluate the effects of aripiprazole compared with other atypical antipsychotics for people with schizophrenia and schizophrenia-like psychoses. SEARCH METHODS: We searched the Cochrane Schizophrenia Group Trials Register (November 2011), inspected references of all identified studies for further trials, and contacted relevant pharmaceutical companies, drug approval agencies and authors of trials for additional information. SELECTION CRITERIA: We included all randomised clinical trials (RCTs) comparing aripiprazole (oral) with oral and parenteral forms of amisulpride, clozapine, olanzapine, quetiapine, risperidone, sertindole, ziprasidone or zotepine for people with schizophrenia or schizophrenia-like psychoses. DATA COLLECTION AND ANALYSIS: We extracted data independently. For dichotomous data we calculated risk ratios (RR) and their 95% confidence intervals (CI) on an intention-to-treat basis based on a random-effects model. Where possible, we calculated illustrative comparative risks for primary outcomes. For continuous data, we calculated mean differences (MD), again based on a random-effects model. We assessed risk of bias for each included study. MAIN RESULTS: We included 12 trials involving 6389 patients. Aripiprazole was compared to olanzapine, risperidone and ziprasidone. All trials were sponsored by an interested drug manufacturer. The overall number of participants leaving studies early was 30% to 40%, limiting validity (no differences between groups).When compared with olanzapine no differences were apparent for global state (no clinically important change: n = 703, 1 RCT, RR short-term 1.00 95% CI 0.81 to 1.22; n = 317, 1 RCT, RR medium-term 1.08 95% CI 0.95 to 1.22) but mental state tended to favour olanzapine (n = 1360, 3 RCTs, MD total Positive and Negative Syndrome Scale (PANSS) 4.68 95% CI 2.21 to 7.16). There was no significant difference in extrapyramidal symptoms (n = 529, 2 RCTs, RR 0.99 95% CI 0.62 to 1.59) but fewer in the aripiprazole group had increased cholesterol levels (n = 223, 1 RCT, RR 0.32 95% CI 0.19 to 0.54) or weight gain of 7% or more of total body weight (n = 1095, 3 RCTs, RR 0.39 95% CI 0.28 to 0.54).When compared with risperidone, aripiprazole showed no advantage in terms of global state (n = 384, 2 RCTs, RR no important improvement 1.14 95% CI 0.81 to 1.60) or mental state (n = 372, 2 RCTs, MD total PANSS 1.50 95% CI -2.96 to 5.96).One study compared aripiprazole with ziprasidone (n = 247) and both the groups reported similar change in the global state (n = 247, 1 RCT, MD average change in Clinical Global Impression-Severity (CGI-S) score -0.03 95% CI -0.28 to 0.22) and mental state (n = 247, 1 RCT, MD change PANSS -3.00 95% CI -7.29 to 1.29).When compared with any one of several new generation antipsychotic drugs the aripiprazole group showed improvement in global state in energy (n = 523, 1 RCT, RR 0.69 95% CI 0.56 to 0.84), mood (n = 523, 1 RCT, RR 0.77 95% CI 0.65 to 0.92), negative symptoms (n = 523, 1 RCT, RR 0.82 95% CI 0.68 to 0.99), somnolence (n = 523, 1 RCT, RR 0.80 95% CI 0.69 to 0.93) and weight gain (n = 523, 1 RCT, RR 0.84 95% CI 0.76 to 0.94). Significantly more people given aripiprazole reported symptoms of nausea (n = 2881, 3 RCTs, RR 3.13 95% CI 2.12 to 4.61) but weight gain (7% or more of total body weight) was less common in people allocated aripiprazole (n = 330, 1 RCT, RR 0.35 95% CI 0.19 to 0.64). Aripiprazole may have value in aggression but data are limited. This will be the focus of another review. AUTHORS' CONCLUSIONS: Information on all comparisons are of limited quality, are incomplete and problematic to apply clinically. Aripiprazole is an antipsychotic drug with a variant but not absent adverse effect profile. Long-term data are sparse and there is considerable scope for another update of this review as new data emerges from the many Chinese studies as well as from ongoing larger, independent pragmatic trials