Cultural Encounters in Iron Age Europe

noCultural encounters form a dominant theme in the study of Iron Age Europe. This was particularly acute in regions where urbanising Mediterranean civilisations came into contact with ‘barbarian’ worlds. This volume presents preliminary work from the ENTRANS Project, which explores the nature and impact of such encounters in south-east Europe, alongside a series of papers on analogous European regions. A range of theoretical perspectives and methodological approaches are offered in an effort to promote dialogue around these central issues in European protohistory.HERAOnly the cover and contents pages are available on Bradford Scholars

Encounters and transformations in Iron Age Europe: the ENTRANS Project

YesThe Iron Age in Europe was a period of tremendous cultural dynamism, during which the values and constructs of urbanised Mediterranean civilisations clashed with alternative webs of identity in ‘barbarian’ temperate Europe. Until recently archaeologists and ancient historians have tended to view the cultural identities of Iron Age Europeans as essentially monolithic (Romans, Greeks, Celts, Illyrians etc). Dominant narratives have been concerned with the supposed origins and spread of peoples, like ‘the Celts’ (e.g. COLLIS 2003), and their subsequent ‘Hellenisation’ or ‘Romanisation’ through encounters with neighbouring societies. Yet there is little to suggest that collective identity in this period was exclusively or predominantly ethnic, national or even tribal. Instead we need to examine the impact of cultural encounters at the more local level of the individual, kin-group or lineage, exploring identity as a more dynamic, layered construct.HERA, European Commissio

Tunability of the elastocaloric response in main-chain liquid crystalline elastomers

Materials exhibiting a large caloric effect could lead to the development of a new generation of heat-management technologies that will have better energy efficiency and be potentially more environmentally friendly. The focus of caloric materials investigations has shifted recently from solid-state materials towards soft materials, such as liquid crystals and liquid crystalline elastomers. It has been shown recently that a large electrocaloric effect exceeding 6 K can be observed in smectic liquid crystals. Here, we report on a significant elastocaloric response observed by direct elastocaloric measurements in main-chain liquid crystal elastomers. It is demonstrated that the character of the nematic to paranematic/isotropic transition can be tuned from the supercritical regime towards the first-order regime, by decreasing the density of crosslinkers. In the latter case, the latent heat additionally enhances the elastocaloric response. Our results indicate that a significant elastocaloric response is present in main-chain liquid crystalline elastomers, driven by stress fields much smaller than in solid elastocaloric materials. Therefore, elastocaloric soft materials can potentially play a significant role as active cooling/heating elements in the development of new heat-management devices

Transcriptomic responses of mixed cultures of ascomycete fungi to lignocellulose using dual RNA-seq reveal inter-species antagonism and limited beneficial effects on CAZyme expression

Gaining new knowledge through fungal monoculture responses to lignocellulose is a widely used approach that can lead to better cocktails for lignocellulose saccharification (the enzymatic release of sugars which are subsequently used to make biofuels). However, responses in lignocellulose mixed cultures are rarely studied in the same detail even though in nature fungi often degrade lignocellulose as mixed communities. Using a dual RNA-seq approach, we describe the first study of the transcriptional responses of wild-type strains of Aspergillus niger, Trichoderma reesei and Penicillium chrysogenum in two and three mixed species shake-flask cultures with wheat straw. Based on quantification of species-specific rRNA, a set of conditions was identified where mixed cultures could be sampled so as to obtain sufficient RNA-seq reads for analysis from each species. The number of differentially-expressed genes varied from a couple of thousand to fewer than one hundred. The proportion of carbohydrate active enzyme (CAZy) encoding transcripts was lower in the majority of the mixed cultures compared to the respective straw monocultures. A small subset of P. chrysogenum CAZy genes showed five to ten-fold significantly increased transcript abundance in a two-species mixed culture with T. reesei. However, a substantial number of T. reesei CAZy transcripts showed reduced abundance in mixed cultures. The highly induced genes in mixed cultures indicated that fungal antagonism was a major part of the mixed cultures. In line with this, secondary metabolite producing gene clusters showed increased transcript abundance in mixed cultures and also mixed cultures with T. reesei led to a decrease in the mycelial biomass of A. niger. Significantly higher monomeric sugar release from straw was only measured using a minority of the mixed culture filtrates and there was no overall improvement. This study demonstrates fungal interaction with changes in transcripts, enzyme activities and biomass in the mixed cultures and whilst there were minor beneficial effects for CAZy transcripts and activities, the competitive interaction between T. reesei and the other fungi was the most prominent feature of this study

Distinctive expansion of gene families associated with plant cell wall degradation, secondary metabolism, and nutrient uptake in the genomes of grapevine trunk pathogens

BackgroundTrunk diseases threaten the longevity and productivity of grapevines in all viticulture production systems. They are caused by distantly-related fungi that form chronic wood infections. Variation in wood-decay abilities and production of phytotoxic compounds are thought to contribute to their unique disease symptoms. We recently released the draft sequences of Eutypa lata, Neofusicoccum parvum and Togninia minima, causal agents of Eutypa dieback, Botryosphaeria dieback and Esca, respectively. In this work, we first expanded genomic resources to three important trunk pathogens, Diaporthe ampelina, Diplodia seriata, and Phaeomoniella chlamydospora, causal agents of Phomopsis dieback, Botryosphaeria dieback, and Esca, respectively. Then we integrated all currently-available information into a genome-wide comparative study to identify gene families potentially associated with host colonization and disease development.ResultsThe integration of RNA-seq, comparative and ab initio approaches improved the protein-coding gene prediction in T. minima, whereas shotgun sequencing yielded nearly complete genome drafts of Dia. ampelina, Dip. seriata, and P. chlamydospora. The predicted proteomes of all sequenced trunk pathogens were annotated with a focus on functions likely associated with pathogenesis and virulence, namely (i) wood degradation, (ii) nutrient uptake, and (iii) toxin production. Specific patterns of gene family expansion were described using Computational Analysis of gene Family Evolution, which revealed lineage-specific evolution of distinct mechanisms of virulence, such as specific cell wall oxidative functions and secondary metabolic pathways in N. parvum, Dia. ampelina, and E. lata. Phylogenetically-informed principal component analysis revealed more similar repertoires of expanded functions among species that cause similar symptoms, which in some cases did not reflect phylogenetic relationships, thereby suggesting patterns of convergent evolution.ConclusionsThis study describes the repertoires of putative virulence functions in the genomes of ubiquitous grapevine trunk pathogens. Gene families with significantly faster rates of gene gain can now provide a basis for further studies of in planta gene expression, diversity by genome re-sequencing, and targeted reverse genetic approaches. The functional validation of potential virulence factors will lead to a more comprehensive understanding of the mechanisms of pathogenesis and virulence, which ultimately will enable the development of accurate diagnostic tools and effective disease management

Encounters and Transformations in Iron Age Europe: the ENTRANS Project

The Iron Age in Europe was a period of tremendous cultural dynamism, during which the values and constructs of urbanised Mediterranean civilisations clashed with alternative webs of identity in ‘barbarian’ temperate Europe. Until recently, archaeologists and ancient historians have tended to view the cultural identities of Iron Age Europeans as essentially monolithic (Romans, Greeks, Celts, Illyrians etc.). Dominant narratives have been concerned with the supposed origins and spread of peoples, such as ‘the Celts’ (e.g. Collis 2003), and their subsequent ‘Hellenisation’ or ‘Romanisation’ through encounters with neighbouring societies. Yet there is little to suggest that collective identity in this period was exclusively or predominantly ethnic, national or even tribal. Instead, we need to examine the impact of cultural encounters at the more local level of the individual, kin-group or lineage, exploring identity as a more dynamic, layered construct

Introduction: cultural encounters and the ENTRANS Project

NoCultural encounters form a dominant theme in the study of Iron Age Europe. This was particularly acute in regions where urbanising Mediterranean civilisations came into contact with ‘barbarian’ worlds. This volume presents preliminary work from the ENTRANS Project, which explores the nature and impact of such encounters in south-east Europe, alongside a series of papers on analogous European regions. A range of theoretical perspectives and methodological approaches are offered in an effort to promote dialogue around these central issues in European protohistory

Kinship practices in Early Iron Age southeast Europe: genetic and isotopic analysis of burials from the Dolge njive barrow cemetery, Dolenjska, Slovenia

DNA analysis demonstrates that all seven individuals buried in an Early Iron Age barrow at Dolge njive, southeast Slovenia, are close biological relatives. Although group composition does not suggest strict adherence to a patrilineal or matrilineal kinship system, the funerary tradition appears highly gendered, with family links through both the male and female line being important in structuring communities. We explore the implications for our understandings of kinship and funerary practices in Early Iron Age southeast Europe

The Archaeologist of the Future is Likely to be a Woman: Age and Gender Patterns in European Archaeology

A recent study into the archaeological profession in 21 European countries resulted in recognising gender equality as a major topic that needs attention. The overall trend is that women will form the future majority of workers in archaeology. However, the conditions under which women work differ by country, and in several countries, women are paid less and are not well represented in leadership positions. Gender equality needs to be put on the agenda and each country should take measurements to close the gap