Brut Dingestow, Buch 1 und 2

Diese Arbeit ist eine Edition der ersten beiden Bücher der Brut Dingestow. Sie enthält neben dem Text und der Übersetzung ein Kommentar, eine Glossar, ein Verzeichnis der Protagonisten, ein Kapitel über die Orthographie sowie eine ausführliche Erörteung der Syntax der Copula- und Uerbum-Substantiuumsätze

Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration

10.7554/eLife.42093.001Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation

Inhibition of acetylcholinesterase in different structures of the rat brain following soman intoxication pretreated with huperzine A

Acetylcholinesterase (AChE) activities in different brain parts were determined quantitatively in rats treated with huperzine A, soman, and huperzine A followed by soman, using histochemical and biochemical methods. Following soman intoxication ( 1.2 x LD50, i.m.), AChE activity was decreased to 30-80% of control values depending on the brain structure. The most sensitive area was the frontal cortex and the most relatively resistant was ncl.ruber. Huperzine A treatment only caused a change in AChE activity varying from 70 to 100% of control values. In rats pretreated with huperzine A and intoxicated with soman, AChE activity was significantly higher than that observed after soman. In these animals, survival of rats pretreated with huperzine was observed while the mortality of unpretreated animals was near to 80%. The results suggest that huperzine A is good candidate for further study for clinical use as a prophylactic drug against nerve agent poisoning

Friction forces position the neural anlage

During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo

The design of a universal stand for connecting automatic tractions to a regulation valve

Bakalářská práce obsahuje návrh univerzálního stojanu pro připojení automatických pohonů k přímému regulačnímu ventilu. Při návrhu byly zohledněny připojovací rozměry automatických pohonů některých výrobců a také konstrukční řešení regulačního pohonu společnosti G-Team a. s. Pevnost stojanu byla ověřena výpočtem MKP v softwaru ANSYS Workbench 14.5.ObhájenoThis bachelor thesis contains the design of universal stand for connection of automatic actuators to a regulation valve. Connection dimensions of automatic actuators and the design of regulation valve of G-Team a. s. company were taken into account during designing. Strength and stability of new design of stand were proved by calculation in ANSYS Workbench 14.5 software

Possibility of heavy metals removal from water

Práce se zabývá možností využití odpadních materiálů obsahujících huminové látky k odstraňování těžkých kovů z vod a možností vitrifikace adsorbentů nasycených těžkými kovy.Dokončená práce s úspěšnou obhajobo

Pokus o přípravu bioodbouratelných objemových materiálů na bázi celulózy a vybraných minerálních plniv

Diplomová práce se zabývá přípravou biodegradabilního materiálu na bázi celulosy a termoplastického škrobu. Příprava materiálu vychází z teoretických podkladů o výrobě a složení výrobků z nasávané kartonáže. Práce dále uvádí, jak dosáhnout u připraveného materiálu hydrofobních vlastností a jak zvýšit jeho pevnost.ObhájenoThe diploma thesis deals with the preparation of biodegradable material based on cellulose and thermoplastic starch. Background of thesis is based on knowledge about paper pulp manufacturing process and composition. There is a way how to get hydrofobic properties and higher tensile strength at the material described in the thesis as well

Positional information and tissue scaling during development and regeneration

In order to contribute to the appropriate tissues during development, cells need to know their position within the embryo. This positional information is conveyed by gradients of signaling molecules, termed morphogens, that are produced in specific regions of the embryo and induce concentration-dependent responses in target tissues. Positional information is remarkably robust, and embryos often develop with the correct proportions even if large parts of the embryo are removed. In this Review, we discuss classical embryological experiments and modern quantitative analyses that have led to mechanistic insights into how morphogen gradients adapt, scale and properly pattern differently sized domains. We analyze these experimental findings in the context of mathematical models and synthesize general principles that apply to multiple systems across species and developmental stages