Factors affecting the transfer of learning to the workplace

Training aims to respond to the needs of development of individuals and organizations (Grohmann & Kauffeld, 2013). Based on Holton model, we carried out a study seeking to identify and understand the factors involved in the process of learning transfer to the workplace from two different training actions on the design and skills. The study took place at a Portuguese organization and involved 98 participants. Former students were interviewed with the purpose to explore the factors that facilitated or hindered the learning transfer, and the Inventory of the Portuguese version of the Learning Transfer System (Holton, Bates, Seyler & Carvalho, 1997; Velada & Caetano, 2009) was applied. The results suggest that the Holton model (2005) shows that the trainees have identified important issues for learning transfer and that there are differences in relation to the transfer factor pursuant to the type of training.info:eu-repo/semantics/publishedVersio

Prognostic implications of comorbidity patterns in critically ill COVID-19 patients: A multicenter, observational study

Background The clinical heterogeneity of COVID-19 suggests the existence of different phenotypes with prognostic implications. We aimed to analyze comorbidity patterns in critically ill COVID-19 patients and assess their impact on in-hospital outcomes, response to treatment and sequelae. Methods Multicenter prospective/retrospective observational study in intensive care units of 55 Spanish hospitals. 5866 PCR-confirmed COVID-19 patients had comorbidities recorded at hospital admission; clinical and biological parameters, in-hospital procedures and complications throughout the stay; and, clinical complications, persistent symptoms and sequelae at 3 and 6 months. Findings Latent class analysis identified 3 phenotypes using training and test subcohorts: low-morbidity (n=3385; 58%), younger and with few comorbidities; high-morbidity (n=2074; 35%), with high comorbid burden; and renal-morbidity (n=407; 7%), with chronic kidney disease (CKD), high comorbidity burden and the worst oxygenation profile. Renal-morbidity and high-morbidity had more in-hospital complications and higher mortality risk than low-morbidity (adjusted HR (95% CI): 1.57 (1.34-1.84) and 1.16 (1.05-1.28), respectively). Corticosteroids, but not tocilizumab, were associated with lower mortality risk (HR (95% CI) 0.76 (0.63-0.93)), especially in renal-morbidity and high-morbidity. Renal-morbidity and high-morbidity showed the worst lung function throughout the follow-up, with renal-morbidity having the highest risk of infectious complications (6%), emergency visits (29%) or hospital readmissions (14%) at 6 months (p<0.01). Interpretation Comorbidity-based phenotypes were identified and associated with different expression of in-hospital complications, mortality, treatment response, and sequelae, with CKD playing a major role. This could help clinicians in day-to-day decision making including the management of post-discharge COVID-19 sequelae. Copyright (C) 2022 The Author(s). Published by Elsevier Ltd

Sin / Sense

Sexto desafío por la erradicación de la violencia contra las mujeres del Institut Universitari d’Estudis Feministes i de Gènere «Purificación Escribano» de la Universitat Jaume

Multisensory Evaluation of Muscle Activity and Human Manipulability during Upper Limb Motor Tasks

In this work, we evaluate the relationship between human manipulability indices obtained from motion sensing cameras and a variety of muscular factors extracted from surface electromyography (sEMG) signals from the upper limb during specific movements that include the shoulder, elbow and wrist joints. The results show specific links between upper limb movements and manipulability, revealing that extreme poses show less manipulability, i.e., when the arms are fully extended or fully flexed. However, there is not a clear correlation between the sEMG signals’ average activity and manipulability factors, which suggests that muscular activity is, at least, only indirectly related to human pose singularities. A possible means to infer these correlations, if any, would be the use of advanced deep learning techniques. We also analyze a set of EMG metrics that give insights into how muscular effort is distributed during the exercises. This set of metrics could be used to obtain good indicators for the quantitative evaluation of sequences of movements according to the milestones of a rehabilitation therapy or to plan more ergonomic and bearable movement phases in a working task.This research was supported by a doctoral fellowship from Fundación Carolina, University of Alicante, and the National Autonomous University of Honduras

A kinematic, imaging and electromyography dataset for human muscular manipulability index prediction

Human Muscular Manipulability is a metric that measures the comfort of an specific pose and it can be used for a variety of applications related to healthcare. For this reason, we introduce KIMHu: a Kinematic, Imaging and electroMyography dataset for Human muscular manipulability index prediction. The dataset is comprised of images, depth maps, skeleton tracking data, electromyography recordings and 3 different Human Muscular Manipulability indexes of 20 participants performing different physical exercises with their arm. The methodology followed to acquire and process the data is also presented for future replication. A specific analysis framework for Human Muscular Manipulability is proposed in order to provide benchmarking tools based on this dataset.Óscar G. Hernández and José M. López Castellanos expresses their gratitude to the Fundación Carolina, the National Autonomous University of Honduras and the University of Alicante, for its funding support while this work was in preparation. This work has also been supported by an University of Alicante Grant GRE-20 (2021/00710/001) and by the Spanish Government Grant PID2019-104818RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”

Self-assembly of 10-N-nonyl acridine orange (NAO) creates molecular zippers promoting cell death by adhesion of membranes

Joint 12th EBSA congress / 10th ICBP – IUPAP congress: Biophysics for life and technology, Madrid, Spain, July 20-24, 2019. -- http://www.ebsa2019.org/ . -- European Biophysics Journal (2019

Polar ammoniostyryls easily converting a clickable lipophilic BODIPY in an advanced plasma membrane probe

7 pags., 5 figs.A very simple, small and symmetric, but highly bright, photostable and functionalizable molecular probe for plasma membrane (PM) has been developed from an accessible, lipophilic and clickable organic dye based on BODIPY. To this aim, two lateral polar ammoniostyryl groups were easily linked to increase the amphiphilicity of the probe and thus its lipid membrane partitioning. Compared to the BODIPY precursor, the transversal diffusion across lipid bilayers of the ammoniostyryled BODIPY probe was highly reduced, as evidenced by fluorescence confocal microscopy on model membranes built up as giant unilamellar vesicles (GUVs). Moreover, the ammoniostyryl groups endow the new BODIPY probe with the ability to optically work (excitation and emission) in the bioimaging-useful red region, as shown by staining of the plasma membrane of living mouse embryonic fibroblasts (MEFs). Upon incubation, this fluorescent probe rapidly entered the cell through the endosomal pathway. By blocking the endocytic trafficking at 4 °C, the probe was confined within the PM of MEFs. Our experiments show the developed ammoniostyrylated BODIPY as a suitable PM fluorescent probe, and confirm the synthetic approach for advancing PM probes, imaging and science.Financial support from MICINN (PID2020-114755GB-C31, -C32 and C-33) and Basque Government (IT1639-22) is gratefully acknowledged by S. d. l. M., J. B. and I. G.-M. I. L.-M. acknowledge financial support from the Spanish Ministry of Science, Innovation and Universities through the grant PGC2018-097903-B-I00. This work was also supported by the TECNOLOGI´AS 2018 program, funded by the Regional Government of Madrid (Grant S2018/BAA-4403 SINOXPHOS-CM, to I. L-M.). S. S.-B. thanks the Regional Government of Madrid for an INVESTIGO contract. M. M.-U´. was recipient of a Sara Borrell fellowship (CD15/00190) financed by the Spanish Ministry of Health

Exacerbated response to oxidative stress in the Retinitis Pigmentosa CerklKD/KO mouse model triggers retinal degeneration pathways upon acute light stress

The retina is particularly vulnerable to genetic and environmental alterations that generate oxidative stress and cause cellular damage in photoreceptors and other retinal neurons, eventually leading to cell death. CERKL (CERamide Kinase-Like) mutations cause Retinitis Pigmentosa and Cone-Rod Dystrophy in humans, two disorders characterized by photoreceptor degeneration and progressive vision loss. CERKL is a resilience gene against oxidative stress, and its overexpression protects cells from oxidative stress-induced apoptosis. Besides, CERKL contributes to stress granule-formation and regulates mitochondrial dynamics in the retina. Using the CerklKD/KO albino mouse model, which recapitulates the human disease, we aimed to study the impact of Cerkl knockdown on stress response and activation of photoreceptor death mechanisms upon light/oxidative stress. After acute light injury, we assessed immediate or late retinal stress response, by combining both omic and non-omic approaches. Our results show that Cerkl knockdown increases ROS levels and causes a basal exacerbated stress state in the retina, through alterations in glutathione metabolism and stress granule production, overall compromising an adequate response to additional oxidative damage. As a consequence, several cell death mechanisms are triggered in CerklKD/KO retinas after acute light stress. Our studies indicate that Cerkl gene is a pivotal player in regulating light-challenged retinal homeostasis and shed light on how mutations in CERKL lead to blindness by dysregulation of the basal oxidative stress response in the retina