82 research outputs found
LÄsta bevis: En nyckelfrÄga? - En studie av krypterat material som bevismedel i brottmÄl
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tkomst till bevismaterial Ă€r avgörande för att förverkliga det straffrĂ€ttsliga ansvaret. Idag Ă€r en stor del av detta bevismaterial digitalt och i syfte att skydda och hemlighĂ„lla sĂ„dan information anvĂ€nds i allt större utstrĂ€ckning kryptering. Kryptering, som enklast möjligt kan sĂ€gas utgöra den digitala versionen av att lĂ„sa in material i ett kassaskĂ„p, innebĂ€r att man utan den sĂ€rskilda dekrypteringsnyckeln inte, Ă„tminstone inte utan omfattande svĂ„righeter, kommer Ă„t det krypterade materialet. NĂ€r en person misstĂ€nkt för brott anvĂ€nt sig av kryptering stĂ€lls rĂ€ttsvĂ€sendet inför svĂ„ra avvĂ€gningar. Samtidigt som tillgĂ„ng till informationen kan vara avgörande för utredningen i mĂ„let utgör det en grundlĂ€ggande rĂ€ttighet under bland annat artikel 6 EKMR att en misstĂ€nkt inte ska behöva belasta sig sjĂ€lv kriminellt och, med andra ord, inte behöva bidra till att material som kan anvĂ€ndas mot honom eller henne kan kommas Ă„t. Denna uppsats undersöker, med utgĂ„ngspunkt i de tvĂ„ grundlĂ€ggande straffprocessrĂ€ttsliga principerna om Ă„ ena sidan förverkligande av det straffrĂ€ttsliga ansvaret och Ă„ andra sidan skydd för individen, tvĂ„ huvudsakliga frĂ„gestĂ€llningar: dels om en order att den misstĂ€nkte ska dekryptera materialet kan vara förenlig med ovannĂ€mnda rĂ€ttighet, dels om material som förblir krypterat Ă€ndĂ„ kan tillmĂ€tas bevisverkan. I uppsatsen argumenteras, i linje med vad som konstaterats i en tidigare studie, för att en dekrypteringsorder riktad mot en misstĂ€nkt under vissa begrĂ€nsade förutsĂ€ttningar kan vara förenlig med rĂ€tten att inte belasta sig sjĂ€lv kriminellt sĂ„ som den utvecklats under artikel 6 EKMR. För att sĂ„ ska vara fallet mĂ„ste det dock vara frĂ„ga om brottslighet dĂ€r ett starkt allmĂ€nt intresse av lagföring föreligger, endast en förhĂ„llandevis lĂ„g grad av tvĂ„ng kan tillĂ„tas och denna mĂ„ste dessutom förenas med starka processuella garantier för den misstĂ€nkte. Vidare konstateras att genomdrivandet av dessa processuella garantier möter sĂ€rskilda utmaningar i ett rĂ€ttsystem som det svenska dĂ€r sĂ„vĂ€l bevisföring som bevisvĂ€rdering Ă€r fri. Utöver dessa rĂ€ttighetsaspekter finns det ocksĂ„ praktiska svĂ„righeter pĂ„ genomförandenivĂ„ att beakta. Sammanfattningsvis bedöms dĂ€rför en dekrypteringsorder riktad mot en misstĂ€nkt, trots att en sĂ„dan bör vara möjlig i vissa situationer, vara av relativt begrĂ€nsat vĂ€rde. Vidare konstateras att sĂ„vĂ€l krypterat som dekrypterat material passar förhĂ„llandevis vĂ€l in under den befintliga regleringen i RB, och det argumenteras för att Ă€ven material som förblir krypterat i vissa fall kan komma att fĂ„ bevisverkan. Vad gĂ€ller material som förblir krypterat rör det sig framförallt om den misstĂ€nktes ovilja att uttala sig om materialet som kan tillmĂ€tas oförmĂ„nlig bevisverkan, Ă€ven om vissa omstĂ€ndigheter direkt hĂ€nförliga till det krypterade materialet ocksĂ„ kan spela in. SĂ„dan oförmĂ„nlig bevisverkan fĂ„r endast förekomma i begrĂ€nsad utstrĂ€ckning och Ă€r i hög grad beroende av de andra omstĂ€ndigheterna i mĂ„let, sĂ„ som exempelvis om viss del av materialet kunnat dekrypteras. Ăven om sĂ„dan oförmĂ„nlig bevisverkan kan accepteras i vissa situationer, argumenteras i denna uppsats för att det i viss mĂ„n framstĂ„r som oklart pĂ„ vilket sĂ€tt det krypterade materialet i praktiken kan komma att pĂ„verka den eventuella domen mot den misstĂ€nkte.Being able to access evidence is crucial for the realisation of responsibility under criminal law. In todayâs world, a large part of what can potentially be evidence is stored digitally. In order to protect and keep digital information confidential, encryption is becoming widespread. Encryption, put simply, can be said to be the digital version of locking material in a safe, and basically means that one cannot, at least not without considerable difficulty, access the material if one does not have access to the decryption key. When a suspect has encrypted material, this means that the judiciary is faced with balancing competing interests: at the same time as access to the material that potentially contains evidence is crucial, it is considered a fundamental right under, among other instruments, article 6 of the ECHR that a suspect should not have to incriminate himâ or herself and, in other words, not have to contribute in order to access said material. This thesis examines, with the principle of realisation of criminal responsibility on the one hand and the principle of protection of the individual in that process on the other, two main questions: whether an order that a suspect should decrypt can be compatible with the privilege against self-incrimination and, if and to what extent material that remains encrypted can be given evidentiary value. It is argued, in line with a previous study, that a decryption order given to a suspect under certain limited circumstances can be compatible with the privilege against self-incrimination as it has developed under article 6 of the ECHR. However, for this to be the case it has to be a matter of a strong public interest, only a relatively low degree of coercion can be used and there has to be strong procedural guarantees for the suspect. These procedural guarantees will mean special challenges in a legal system like the Swedish, where both the production and evaluation of evidence is free. In addition, there are also a number of practical issues as to whether such a decryption order will have any effect. In sum, a decryption order given to a suspect, though under certain conditions possible, is therefore considered to be of a relatively limited value. Further it is argued that decrypted as well as encrypted material fits rather well into the categorization of evidence under the Swedish code of judicial procedure, and that also material that remains encrypted under certain circumstances may be given evidentiary value at trial. When this becomes relevant it is primarily the suspectâs resistance to comment on the material or explain its existence that can possibly lead to adverse inference, even though some aspects relating directly to the material itself can also become relevant. However, this can only be allowed in a limited number of situations, and is highly dependent on the other circumstances of the case. For example this could be whether any of the material has been decrypted and what it shows. Even though such evidentiary value can sometimes be acceptable, this thesis argues that it seems to remain partly unclear how the encrypted material would practically affect the trial
Entwicklungszusammenarbeit im Rahmen der Vereinten Nationen
Die Vereinten Nationen
(United Nations â UN) setzten sich in
ihrer Charta (Art.1) das Ziel, âinternationale
Probleme wirtschaftlicher,
sozialer, kultureller und humanitÀrer Art
zu lösenâ. Auf dieser Grundlage sind
sie seit ihrer GrĂŒndung in vielfĂ€ltiger
Weise entwicklungspolitisch tÀtig. Heute
richten sich zum Teil groĂe Erwartungen
an die UN, die im Sinne von âglobal
governanceâ in diesem Bereich eine zentrale
Rolle ĂŒbernehmen sollen. Allerdings
besitzen die UN bislang nicht die Voraussetzungen,
um eine solche Rolle spielen zu
können. Nicht nur die Vielzahl und die
UnĂŒbersichtlichkeit der UN-Einrichtungen
auf diesem Gebiet geben immer
wieder Anlass zu erheblicher Kritik. Auch
andere (Vor-)Urteile wie Ineffizienz und
Verschwendung, SchwerfÀlligkeit und
Politisierung gehören vielfach weiterhin
zum Image der UN-Entwicklungszusammenarbeit.
Hinzu kommt eine geringe
finanzielle Ausstattung. Allerdings haben
die Reformanstrengungen von UN-GeneralsekretÀr
Kofi Annan durchaus zu
sichtbaren Erfolgen gefĂŒhrt. Bestehen
bleiben aber die unterschiedlichen
Interessen und Erwartungen der sehr
heterogenen 189 UN-Mitgliedstaaten
Molecular Organisation of Tick-Borne Encephalitis Virus
Tick-borne encephalitis virus (TBEV) is a pathogenic, enveloped, positive-stranded RNA virus in the family Flaviviridae. Structural studies of flavivirus virions have primarily focused on mosquito-borne species, with only one cryo-electron microscopy (cryo-EM) structure of a tick-borne species published. Here, we present a 3.3 Ă
cryo-EM structure of the TBEV virion of the Kuutsalo-14 isolate, confirming the overall organisation of the virus. We observe conformational switching of the peripheral and transmembrane helices of M protein, which can explain the quasi-equivalent packing of the viral proteins and highlights their importance in stabilising membrane protein arrangement in the virion. The residues responsible for M protein interactions are highly conserved in TBEV but not in the structurally studied Hypr strain, nor in mosquito-borne flaviviruses. These interactions may compensate for the lower number of hydrogen bonds between E proteins in TBEV compared to the mosquito-borne flaviviruses. The structure reveals two lipids bound in the E protein which are important for virus assembly. The lipid pockets are comparable to those recently described in mosquito-borne Zika, Spondweni, Dengue, and Usutu viruses. Our results thus advance the understanding of tick-borne flavivirus architecture and virion-stabilising interactions
Molecular Organisation of Tick-Borne Encephalitis Virus
Tick-borne encephalitis virus (TBEV) is a pathogenic, enveloped, positive-stranded RNA virus in the family Flaviviridae. Structural studies of flavivirus virions have primarily focused on mosquito-borne species, with only one cryo-electron microscopy (cryo-EM) structure of a tick-borne species published. Here, we present a 3.3 Ă
cryo-EM structure of the TBEV virion of the Kuutsalo-14 isolate, confirming the overall organisation of the virus. We observe conformational switching of the peripheral and transmembrane helices of M protein, which can explain the quasi-equivalent packing of the viral proteins and highlights their importance in stabilising membrane protein arrangement in the virion. The residues responsible for M protein interactions are highly conserved in TBEV but not in the structurally studied Hypr strain, nor in mosquito-borne flaviviruses. These interactions may compensate for the lower number of hydrogen bonds between E proteins in TBEV compared to the mosquito-borne flaviviruses. The structure reveals two lipids bound in the E protein which are important for virus assembly. The lipid pockets are comparable to those recently described in mosquito-borne Zika, Spondweni, Dengue, and Usutu viruses. Our results thus advance the understanding of tick-borne flavivirus architecture and virion-stabilising interactions
Structures of enveloped virions determined by cryogenic electron microscopy and tomography : Advances in Virus Research
Enveloped viruses enclose their genomes inside a lipid bilayer which is decorated by membrane proteins that mediate virus entry. These viruses display a wide range of sizes, morphologies and symmetries. Spherical viruses are often isometric and their envelope proteins follow icosahedral symmetry. Filamentous and pleomorphic viruses lack such global symmetry but their surface proteins may display locally ordered assemblies. Determining the structures of enveloped viruses, including the envelope proteins and their protein-protein interactions on the viral surface, is of paramount importance. These structures can reveal how the virions are assembled and released by budding from the infected host cell, how the progeny virions infect new cells by membrane fusion, and how antibodies bind surface epitopes to block infection. In this chapter, we discuss the uses of cryogenic electron microscopy (cryo-EM) in elucidating structures of enveloped virions. Starting from a detailed outline of data collection and processing strategies, we highlight how cryo-EM has been successfully utilized to provide unique insights into enveloped virus entry, assembly, and neutralization.Peer reviewe
Echo cancellation in ADSL modems
This Master Thesis report presents a way of enhancing the performance of an ADSL (Asymmetric Digital Subscriber Line) system by canceling out transmitter echoes that occur in the coupling between the AFE (Analog Front End) and the phone lines. This new way of canceling the transmit echoes applies to all systems where the transmitted signal can be modeled by a random noise signal. The idea is to cancel out the transmit echoes in the analog domain before the ADC (Analog Digital Converter) in order to increase the sensitivity of the receiver. With this echo canceling method the echo suppression of the transmitted signal can come close to what is theoretically possible. The development and simulations are done using a Matlab model of the AFE. To verify the model and show that the performance expectations were fulfilled the echo canceller was implemented in a real time system based on four ADSP-21160 DSPs (Digital Signal Processors) from ADI (Analog Devices). The issues involved with a migration from a simulation model to real systems are also considered.Validerat; 20101217 (root
Uukuniemi virus-like particles : a model system for bunyaviral assembly
Viruses are intracellular parasites that are unable to multiply except
when inside a living cell of a host. Outside their host they remain
inert. There are many different types of viruses that are classified into
different virus families according to their size, nucleic acid
composition (RNA or DNA), or the composition of their outer shell (naked
or enveloped). Following entry, negative stranded viruses release their
genome which is subsequently replicated, transcribed, and the different
viral components are assembled into new virus particles, which are
released from the host cell and ready to infect new cells. This thesis
focuses on how the different viral parts assemble into an infectious
particle inside the cell as well as on the identification of parts in the
RNA genome important for viral replication.
Uukuniemi virus (UUKV), a prototypic member of the Bunyaviridae family,
has a segmented RNA genome with negative polarity. The three segments
encode four structural proteins, two glycoproteins (GN and GC) located in
the envelope, one nucleoprotein (N protein), and a RNA-dependent RNA
polymerase (L protein). Flanking these open reading frames (ORFs) are
non-coding regions (NCRs) containing cis-acting signals important for
viral transcription, replication, encapsidation and packaging. These NCRs
encompass one variable region, and one highly conserved region located in
both the 5 and 3 terminal ends of the three segments, which are
complementary to each other.
To study the function of the NCRs we used a minigenome system developed
for UUKV. In this system the viral protein coding sequence is replaced by
sequences encoding a reporter protein. This minigenome is transfected
into cells together with the N and L protein necessary for replication
and transcription, after which reporter protein expression can be
measured. In the first paper we studied the variable region of the NCRs
and its effect on promoter strength and packaging efficiency. We
performed this by comparing the activity of minigenomes that contained
the NCRs derived from all three different segments. We found that the
variable region is not only important for the regulation of promoter
activity but also for packaging efficiency, since the three different
minigenomes all showed different reporter activity.
Next the assembly of the UUKV inside the cell was examined. In order to
study packaging and assembly, we first developed a virus-like particle
(VLP) system for UUKV. With the addition of the glycoprotein precursor
encoding both glycoproteins GN and GC to the minigenome system, VLPs
containing the minigenome are produced and released into the supernatant.
These particles are able to infect new cells where reporter protein
expression can be detected. Characterization of these VLPs revealed that
they have similar size and surface morphology as wild-type UUKV. Moreover
we demonstrated that only the two glycoproteins are required for
generating VLPs, suggesting that UUKV budding is driven by the two
glycoproteins.
We further analyzed the importance of specific amino acids in the
cytoplasmic tail of both glycoproteins, GN and GC, for packaging of the
RNA genome into VLPs and the budding of UUKV. This was done by performing
an alanine scan of the GN cytoplasmic tail (81 amino acids) and the GC
cytoplasmic tail (5 amino acids), and analyzing the effect of these
mutations on particle formation in our VLP system. We identified three
regions in the GN tail (amino acids 21-25, 46-50 and 71-81) that are
important for minigenome transfer of the VLPs. A more detailed analysis
showed, four amino acids in the GN cytoplasmic tail involved in the
packaging interaction with the ribonucleoproteins while two other amino
acids are important for the budding into the Golgi compartment. Finally
three amino acids in the GN and two in the GC cytoplasmic tail are
important for the correct localization of the two glycoproteins in the
cell.
In conclusion, we show a mechanism of UUKV assembly and demonstrate the
usefulness of our experimental system. We also postulate that the VLP
system is a useful tool for analyzing packaging, assembly, and budding
for other members of the Bunyavidae family
ï»żï»żï»żA study of a low energy house - methods for improvning energy performance while maintaining cost efficiency
 diskuterats flitigt, sĂ„vĂ€l pĂ„ det nationella planet som pĂ„ EU-nivĂ„. Trenden pekar pĂ„ ett byggande dĂ€r allt större vikt lĂ€ggs vid den byggda miljöns energiprestanda. Redan i dagslĂ€get finns flera exempel pĂ„ hus med ett mycket lĂ„gt energibehov, och antalet lĂ„genergihus vĂ€xer för varje Ă„r. I skrivande stund ligger kraven pĂ„ bostĂ€ders specifika energianvĂ€ndning pĂ„ 110 kWh/m 2 Ă„r i Stockholm, men redan till nĂ€sta Ă„r finns förslag pĂ„ att minska motsvarande siffra till 90. Energimyndigheten genomför just nu ett arbete med att tolka EU:s direktiv om sĂ„ kallade NĂ€ra nollenergihus och de preliminĂ€ra resultaten indikerar att kravnivĂ„n för köpt energi kommer hamna kring 55 kWh/m 2 Ă„r, vilket Ă€r i nivĂ„ med de rekommendationer som i dag Ă„terfinns i FEBY:s Kravspecifikation för Passivhus. Med utgĂ„ngslĂ€ge i ett uppfört lĂ„genergihus i Henriksdalshamnen i Stockholm har vi i arbetet analyserat olika energieffektiviserande Ă„tgĂ€rders inverkan pĂ„ effekt- och energibehov. Ăven de ekonomiska aspekterna förknippade med Ă„tgĂ€rderna har studerats med hjĂ€lp av en modell för livscykelkostnader. Ă
tgĂ€rderna studerades inledningsvis individuellt för att ge en bild av hur stor pĂ„verkan de har var för sig. Tre Ă„tgĂ€rdspaket sattes sedan samman, dĂ€r de mest energi- och kostnadseffektiva lösningarna ingick. Det första Ă„tgĂ€rdspaketet syftade till att ta huset till passivhusnivĂ„, vilket uppnĂ„ddes genom byte av fönster samt en förbĂ€ttring av lufttĂ€theten. Den ekonomiska analysen visade att detta paket inte var lönsamt. Det andra Ă„tgĂ€rdspaketet hade som mĂ„l att sĂ„ mycket som möjligt sĂ€nka byggnadens energibehov och samtidigt uppvisa lönsamhet. Resultatet blev ett mer Ă€n halverat energibehov och den ekonomiska kalkylen pekade pĂ„ att det Ă€ven gick med vinst. Slutligen studerades möjligheten att installera energiproducerande system för att kunna ta huset till ett plusenergiutförande. Tanken var att undersöka om det var möjligt att pĂ„ ett ekonomiskt försvarbart sĂ€tt tĂ€cka upp behovet av köpt energi med hjĂ€lp av sol- och vindkraft. Analysen visar att det i dagslĂ€get skulle vara svĂ„rt att uppnĂ„, frĂ€mst pĂ„ grund av de stora investeringskostnader som Ă„tgĂ€rderna medför, samt de hinder som Ă€r förknippade med regelverken för att sĂ€lja energi.todayâs recommendations found in FEBY's Kravspecifikation för Passivhus.   Abstract Energy and environmental demands regarding buildings have become an increasingly discussed topic, both in Sweden and in Europe as a whole. The general trend indicates that greater efforts are being put into the energy efficiency of the built environment. There are already numerous examples of houses with a low energy demand, and the number of low energy buildings is constantly growing. At the time being, the maximum level for energy demands for housing in Stockholm is 110 kWh/m 2 year, but as soon as next year the limit will be changed to 90. The Swedish agency Energimyndigheten is currently conducting a project to interpret the EU Directive on the so-called Nearly zero energy buildings, and the preliminary results indicate that the level of requirements for purchased energy will end up with about 55 kWh / m 2 year, which is in line with todayâs recommendations found in FEBY's Kravspecifikation för Passivhus. Starting with a low-energy house in Henriksdalshamnen in Stockholm, we have analyzed various energy-efficiency measures and their influence on power and energy needs. The financial aspects associated with the measures have been studied using a model of life cycle costs. The measures were initially studied individually to give an idea of how much impact they each had. Three packages of measures were then put together, where the most energyand cost-effective solutions were included. The first package of measures aimed to achieve the standard for Passive houses, and was accomplished by the replacement of windows and an improvement in airtightness. The economic analysis showed that this package was not viable. The second package of measures aimed to as much as possible reduce the building's energy needs and at the same time demonstrate profitability. The results showed a more than halved energy demand and the economic calculations indicated that it even turned a profit. Finally, the possibility of installing energy-producing systems in order to achieve a plus energy house was studied. The idea was to examine whether it was possible to cover up the need for purchased energy by using solar and wind power, while maintaining profitability. The analysis shows that this would be difficult to achieve, primarily because of the high investment costs entailed, and the difficulties associated with the regulations regarding the sale of energy
Bibliotekssystemet pÄ Jamaika
Föreliggande specialarbete tar upp och behandlar bibliotekssystemet pĂ„ Jamaika. Upppsatsskrivarna redogör för folk-, special- och forskningsbibliotek utifrĂ„n sina egna erfarenheter under praktiktiden pĂ„ Jamaika nĂ„gra mĂ„nader vintern 1981/82. FramstĂ€llningen kompletteras av ett rikt bildmaterial och frĂ„geformulĂ€r som besvarades av bibliotekarierna vid de olika studiebesöken.Obs! Ă.h.t. dĂ„ligt tryck. Vissa sidor saknar paginering.</p
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