Streaming visualisation of quantitative mass spectrometry data based on a novel raw signal decomposition method

As data rates rise, there is a danger that informatics for high-throughput LC-MS becomes more opaque and inaccessible to practitioners. It is therefore critical that efficient visualisation tools are available to facilitate quality control, verification, validation, interpretation, and sharing of raw MS data and the results of MS analyses. Currently, MS data is stored as contiguous spectra. Recall of individual spectra is quick but panoramas, zooming and panning across whole datasets necessitates processing/memory overheads impractical for interactive use. Moreover, visualisation is challenging if significant quantification data is missing due to data-dependent acquisition of MS/MS spectra. In order to tackle these issues, we leverage our seaMass technique for novel signal decomposition. LC-MS data is modelled as a 2D surface through selection of a sparse set of weighted B-spline basis functions from an over-complete dictionary. By ordering and spatially partitioning the weights with an R-tree data model, efficient streaming visualisations are achieved. In this paper, we describe the core MS1 visualisation engine and overlay of MS/MS annotations. This enables the mass spectrometrist to quickly inspect whole runs for ionisation/chromatographic issues, MS/MS precursors for coverage problems, or putative biomarkers for interferences, for example. The open-source software is available from http://seamass.net/viz/

Compression of Stereo Disparity Streams Using Wavelets and Optical Flow

Recent advances in computing have enabled fast reconstructions of dynamic scenes from multiple images. However, the efficient coding of changing 3D-data has hardly been addressed. Progressive geometric compression and streaming are based on static data sets which are mostly artificial or obtained from accurate range sensors. In this paper, we present a system for efficient coding of 3D-data which are given in forms of 2 + 1/2 disparity maps. Disparity maps are spatially coded using wavelets and temporally predicted by computing flow. The resulted representation of a 3D-stream consists then of spatial wavelet coefficients, optical flow vectors, and disparity differences between predicted and incoming image. The approach has also very useful by-products: disparity predictions can significantly reduce the disparity search range and if appropriately modeled increase the accuracy of depth estimation

Self-Potential Method to Assess Embankment Stability: A Study related to the Sidoarjo Mud Flow

The stability of an embankment is generally influenced by a number of factors, such as deformation, fractures, overtopping, seepage, etc. Fractures and seepage are commonly found in the LUSI (Sidoarjo mud flow) embankment. In this study, analysis of self-potential (SP) data was applied to identify fractures and seepage in the LUSI embankment. Noise-Assisted Multivariate Empirical Mode Decomposition (NA-MEMD) and Continuous Wavelet Transform (CWT) were applied to determine the location of seepage and fractures in the subsurface based on SP data. The results were correlated with the 2D direct current resistivity (DCR) method, which showed that both methods worked well and were compatible in detecting and localizing fracture and seepage in the LUSI embankment

A comparative evaluation of 3 different free-form deformable image registration and contour propagation methods for head and neck MRI : the case of parotid changes radiotherapy

Purpose: To validate and compare the deformable image registration and parotid contour propagation process for head and neck magnetic resonance imaging in patients treated with radiotherapy using 3 different approachesthe commercial MIM, the open-source Elastix software, and an optimized version of it. Materials and Methods: Twelve patients with head and neck cancer previously treated with radiotherapy were considered. Deformable image registration and parotid contour propagation were evaluated by considering the magnetic resonance images acquired before and after the end of the treatment. Deformable image registration, based on free-form deformation method, and contour propagation available on MIM were compared to Elastix. Two different contour propagation approaches were implemented for Elastix software, a conventional one (DIR_Trx) and an optimized homemade version, based on mesh deformation (DIR_Mesh). The accuracy of these 3 approaches was estimated by comparing propagated to manual contours in terms of average symmetric distance, maximum symmetric distance, Dice similarity coefficient, sensitivity, and inclusiveness. Results: A good agreement was generally found between the manual contours and the propagated ones, without differences among the 3 methods; in few critical cases with complex deformations, DIR_Mesh proved to be more accurate, having the lowest values of average symmetric distance and maximum symmetric distance and the highest value of Dice similarity coefficient, although nonsignificant. The average propagation errors with respect to the reference contours are lower than the voxel diagonal (2 mm), and Dice similarity coefficient is around 0.8 for all 3 methods. Conclusion: The 3 free-form deformation approaches were not significantly different in terms of deformable image registration accuracy and can be safely adopted for the registration and parotid contour propagation during radiotherapy on magnetic resonance imaging. More optimized approaches (as DIR_Mesh) could be preferable for critical deformations

AMM: Adaptive Multilinear Meshes

We present Adaptive Multilinear Meshes (AMM), a new framework that significantly reduces the memory footprint compared to existing data structures. AMM uses a hierarchy of cuboidal cells to create continuous, piecewise multilinear representation of uniformly sampled data. Furthermore, AMM can selectively relax or enforce constraints on conformity, continuity, and coverage, creating a highly adaptive and flexible representation to support a wide range of use cases. AMM supports incremental updates in both spatial resolution and numerical precision establishing the first practical data structure that can seamlessly explore the tradeoff between resolution and precision. We use tensor products of linear B-spline wavelets to create an adaptive representation and illustrate the advantages of our framework. AMM provides a simple interface for evaluating the function defined on the adaptive mesh, efficiently traversing the mesh, and manipulating the mesh, including incremental, partial updates. Our framework is easy to adopt for standard visualization and analysis tasks. As an example, we provide a VTK interface, through efficient on-demand conversion, which can be used directly by corresponding tools, such as VisIt, disseminating the advantages of faster processing and a smaller memory footprint to a wider audience. We demonstrate the advantages of our approach for simplifying scalar-valued data for commonly used visualization and analysis tasks using incremental construction, according to mixed resolution and precision data streams