10,981 research outputs found
The Regime-Dependent Determination of Credibility: A New Look at European Interest Rate Differentials
Once you allow for persistence in macroeconomic variables, two aspects of exchange rate credibility emerge whose relative importance can vary over time. Hence, the effect of policy measures on interest rate differentials becomes ambiguous. In this paper, a Markov-switching VAR that allows for parameter shifts across regimes is employed to test the hypothesis of regime-dependent determination of credibility for major EMS countries. The model separates two regimes that are distinct with respect to the time series properties of the interest rate spread. Regime-dependent impulse response functions reveal substantial differences in the response of spreads to macroeconomic shocks across regimes.Regime-switching; VAR; interest rate differentials; regime-dependent impulse response functions; credibility
A stochastic and dynamical view of pluripotency in mouse embryonic stem cells
Pluripotent embryonic stem cells are of paramount importance for biomedical
research thanks to their innate ability for self-renewal and differentiation
into all major cell lines. The fateful decision to exit or remain in the
pluripotent state is regulated by complex genetic regulatory network. Latest
advances in transcriptomics have made it possible to infer basic topologies of
pluripotency governing networks. The inferred network topologies, however, only
encode boolean information while remaining silent about the roles of dynamics
and molecular noise in gene expression. These features are widely considered
essential for functional decision making. Herein we developed a framework for
extending the boolean level networks into models accounting for individual
genetic switches and promoter architecture which allows mechanistic
interrogation of the roles of molecular noise, external signaling, and network
topology. We demonstrate the pluripotent state of the network to be a broad
attractor which is robust to variations of gene expression. Dynamics of exiting
the pluripotent state, on the other hand, is significantly influenced by the
molecular noise originating from genetic switching events which makes cells
more responsive to extracellular signals. Lastly we show that steady state
probability landscape can be significantly remodeled by global gene switching
rates alone which can be taken as a proxy for how global epigenetic
modifications exert control over stability of pluripotent states.Comment: 11 pages, 7 figure
The interplay between discrete noise and nonlinear chemical kinetics in a signal amplification cascade
We used various analytical and numerical techniques to elucidate signal
propagation in a small enzymatic cascade which is subjected to external and
internal noise. The nonlinear character of catalytic reactions, which underlie
protein signal transduction cascades, renders stochastic signaling dynamics in
cytosol biochemical networks distinct from the usual description of stochastic
dynamics in gene regulatory networks. For a simple 2-step enzymatic cascade
which underlies many important protein signaling pathways, we demonstrated that
the commonly used techniques such as the linear noise approximation and the
Langevin equation become inadequate when the number of proteins becomes too
low. Consequently, we developed a new analytical approximation, based on mixing
the generating function and distribution function approaches, to the solution
of the master equation that describes nonlinear chemical signaling kinetics for
this important class of biochemical reactions. Our techniques work in a much
wider range of protein number fluctuations than the methods used previously. We
found that under certain conditions the burst-phase noise may be injected into
the downstream signaling network dynamics, resulting possibly in unusually
large macroscopic fluctuations. In addition to computing first and second
moments, which is the goal of commonly used analytical techniques, our new
approach provides the full time-dependent probability distributions of the
colored non-Gaussian processes in a nonlinear signal transduction cascade.Comment: 16 pages, 9 figure
Towards the timely detection of toxicants
We address the problem of enhancing the sensitivity of biosensors to the
influence of toxicants, with an entropy method of analysis, denoted as
CASSANDRA, recently invented for the specific purpose of studying
non-stationary time series. We study the specific case where the toxicant is
tetrodotoxin. This is a very poisonous substance that yields an abrupt drop of
the rate of spike production at t approximatively 170 minutes when the
concentration of toxicant is 4 nanomoles. The CASSANDRA algorithm reveals the
influence of toxicants thirty minutes prior to the drop in rate at a
concentration of toxicant equal to 2 nanomoles. We argue that the success of
this method of analysis rests on the adoption of a new perspective of
complexity, interpreted as a condition intermediate between the dynamic and the
thermodynamic state.Comment: 6 pages and 3 figures. Accepted for publication in the special issue
of Chaos Solitons and Fractal dedicated to the conference "Non-stationary
Time Series: A Theoretical, Computational and Practical Challenge", Center
for Nonlinear Science at University of North Texas, from October 13 to
October 19, 2002, Denton, TX (USA
Cellular signaling networks function as generalized Wiener-Kolmogorov filters to suppress noise
Cellular signaling involves the transmission of environmental information
through cascades of stochastic biochemical reactions, inevitably introducing
noise that compromises signal fidelity. Each stage of the cascade often takes
the form of a kinase-phosphatase push-pull network, a basic unit of signaling
pathways whose malfunction is linked with a host of cancers. We show this
ubiquitous enzymatic network motif effectively behaves as a Wiener-Kolmogorov
(WK) optimal noise filter. Using concepts from umbral calculus, we generalize
the linear WK theory, originally introduced in the context of communication and
control engineering, to take nonlinear signal transduction and discrete
molecule populations into account. This allows us to derive rigorous
constraints for efficient noise reduction in this biochemical system. Our
mathematical formalism yields bounds on filter performance in cases important
to cellular function---like ultrasensitive response to stimuli. We highlight
features of the system relevant for optimizing filter efficiency, encoded in a
single, measurable, dimensionless parameter. Our theory, which describes noise
control in a large class of signal transduction networks, is also useful both
for the design of synthetic biochemical signaling pathways, and the
manipulation of pathways through experimental probes like oscillatory input.Comment: 15 pages, 5 figures; to appear in Phys. Rev.
The stochastic behavior of a molecular switching circuit with feedback
Background: Using a statistical physics approach, we study the stochastic
switching behavior of a model circuit of multisite phosphorylation and
dephosphorylation with feedback. The circuit consists of a kinase and
phosphatase acting on multiple sites of a substrate that, contingent on its
modification state, catalyzes its own phosphorylation and, in a symmetric
scenario, dephosphorylation. The symmetric case is viewed as a cartoon of
conflicting feedback that could result from antagonistic pathways impinging on
the state of a shared component.
Results: Multisite phosphorylation is sufficient for bistable behavior under
feedback even when catalysis is linear in substrate concentration, which is the
case we consider. We compute the phase diagram, fluctuation spectrum and
large-deviation properties related to switch memory within a statistical
mechanics framework. Bistability occurs as either a first-order or second-order
non-equilibrium phase transition, depending on the network symmetries and the
ratio of phosphatase to kinase numbers. In the second-order case, the circuit
never leaves the bistable regime upon increasing the number of substrate
molecules at constant kinase to phosphatase ratio.
Conclusions: The number of substrate molecules is a key parameter controlling
both the onset of the bistable regime, fluctuation intensity, and the residence
time in a switched state. The relevance of the concept of memory depends on the
degree of switch symmetry, as memory presupposes information to be remembered,
which is highest for equal residence times in the switched states.
Reviewers: This article was reviewed by Artem Novozhilov (nominated by Eugene
Koonin), Sergei Maslov, and Ned Wingreen.Comment: Version published in Biology Direct including reviewer comments and
author responses, 28 pages, 7 figure
Collective and single cell behavior in epithelial contact inhibition
Control of cell proliferation is a fundamental aspect of tissue physiology
central to morphogenesis, wound healing and cancer. Although many of the
molecular genetic factors are now known, the system level regulation of growth
is still poorly understood. A simple form of inhibition of cell proliferation
is encountered in vitro in normally differentiating epithelial cell cultures
and is known as "contact inhibition". The study presented here provides a
quantitative characterization of contact inhibition dynamics on tissue-wide and
single cell levels. Using long-term tracking of cultured MDCK cells we
demonstrate that inhibition of cell division in a confluent monolayer follows
inhibition of cell motility and sets in when mechanical constraint on local
expansion causes divisions to reduce cell area. We quantify cell motility and
cell cycle statistics in the low density confluent regime and their change
across the transition to epithelial morphology which occurs with increasing
cell density. We then study the dynamics of cell area distribution arising
through reductive division, determine the average mitotic rate as a function of
cell size and demonstrate that complete arrest of mitosis occurs when cell area
falls below a critical value. We also present a simple computational model of
growth mechanics which captures all aspects of the observed behavior. Our
measurements and analysis show that contact inhibition is a consequence of
mechanical interaction and constraint rather than interfacial contact alone,
and define quantitative phenotypes that can guide future studies of molecular
mechanisms underlying contact inhibition
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