Modelling mitral valvular dynamics–current trend and future directions

Dysfunction of mitral valve causes morbidity and premature mortality and remains a leading medical problem worldwide. Computational modelling aims to understand the biomechanics of human mitral valve and could lead to the development of new treatment, prevention and diagnosis of mitral valve diseases. Compared with the aortic valve, the mitral valve has been much less studied owing to its highly complex structure and strong interaction with the blood flow and the ventricles. However, the interest in mitral valve modelling is growing, and the sophistication level is increasing with the advanced development of computational technology and imaging tools. This review summarises the state-of-the-art modelling of the mitral valve, including static and dynamics models, models with fluid-structure interaction, and models with the left ventricle interaction. Challenges and future directions are also discussed

Array atomic force microscopy for real-time multiparametric analysis.

Nanoscale multipoint structure-function analysis is essential for deciphering the complexity of multiscale biological and physical systems. Atomic force microscopy (AFM) allows nanoscale structure-function imaging in various operating environments and can be integrated seamlessly with disparate probe-based sensing and manipulation technologies. Conventional AFMs only permit sequential single-point analysis; widespread adoption of array AFMs for simultaneous multipoint study is challenging owing to the intrinsic limitations of existing technological approaches. Here, we describe a prototype dispersive optics-based array AFM capable of simultaneously monitoring multiple probe-sample interactions. A single supercontinuum laser beam is utilized to spatially and spectrally map multiple cantilevers, to isolate and record beam deflection from individual cantilevers using distinct wavelength selection. This design provides a remarkably simplified yet effective solution to overcome the optical cross-talk while maintaining subnanometer sensitivity and compatibility with probe-based sensors. We demonstrate the versatility and robustness of our system on parallel multiparametric imaging at multiscale levels ranging from surface morphology to hydrophobicity and electric potential mapping in both air and liquid, mechanical wave propagation in polymeric films, and the dynamics of living cells. This multiparametric, multiscale approach provides opportunities for studying the emergent properties of atomic-scale mechanical and physicochemical interactions in a wide range of physical and biological networks

Correlated Multimodal Imaging in Life Sciences:Expanding the Biomedical Horizon

International audienceThe frontiers of bioimaging are currently being pushed toward the integration and correlation of several modalities to tackle biomedical research questions holistically and across multiple scales. Correlated Multimodal Imaging (CMI) gathers information about exactly the same specimen with two or more complementary modalities that-in combination-create a composite and complementary view of the sample (including insights into structure, function, dynamics and molecular composition). CMI allows to describe biomedical processes within their overall spatio-temporal context and gain a mechanistic understanding of cells, tissues, diseases or organisms by untangling their molecular mechanisms within their native environment. The two best-established CMI implementations for small animals and model organisms are hardware-fused platforms in preclinical imaging (Hybrid Imaging) and Correlated Light and Electron Microscopy (CLEM) in biological imaging. Although the merits of Preclinical Hybrid Imaging (PHI) and CLEM are well-established, both approaches would benefit from standardization of protocols, ontologies and data handling, and the development of optimized and advanced implementations. Specifically, CMI pipelines that aim at bridging preclinical and biological imaging beyond CLEM and PHI are rare but bear great potential to substantially advance both bioimaging and biomedical research. CMI faces three mai

The Importance of the Instantaneous Phase in Detecting Faces with Convolutional Neural Networks

Convolutional Neural Networks (CNN) have provided new and accurate methods for processing digital images and videos. Yet, training CNNs is extremely demanding in terms of computational resources. Also, for simple applications, the standard use of transfer learning also tends to require far more resources than what may be needed. Furthermore, the final systems tend to operate as black boxes that are difficult to interpret. The current thesis considers the problem of detecting faces from the AOLME video dataset. The AOLME dataset consists of a large video collection of group interactions that are recorded in unconstrained classroom environments. For the thesis, still image frames were extracted at every minute from 18 24-minute videos. Then, each video frame was divided into 9x5 blocks with 50x50 pixels each. For each of the 19440 blocks, the percentage of face pixels was set as ground truth. Face detection was then defined as a regression problem for determining the face pixel percentage for each block. For testing different methods, 12 videos were used for training and validation. The remaining 6 videos were used for testing. The thesis examines the impact of using the instantaneous phase for the AOLME block-based face detection application. For comparison, the thesis compares the use of the Frequency modulation image based on the instantaneous phase, the use of the instantaneous amplitude, and the original gray scale image. To generate the FM and AM inputs, the thesis uses dominant component analysis that aims to decrease the training overhead while maintaining interpretability. The results indicate that the use of the FM image yielded about the same performance as the MobileNet V2 architecture (AUC of 0.78 vs 0.79), with vastly reduced training times. Training was 7x faster for an Intel Xeon with a GTX 1080 based desktop and 11x faster on a laptop with Intel i5 with a GTX 1050. Furthermore, the proposed architecture trains 123x less parameters than what is needed for MobileNet V2. The FM-based neural network architecture uses a single convolutional layer. In comparison, the full LeNet-5 on the same image block using the original image could not be trained for face detection (AUC of 0.5)

The Importance of the Instantaneous Phase in Detecting Faces with Convolutional Neural Networks

Convolutional Neural Networks (CNN) have provided new and accurate methods for processing digital images and videos. Yet, training CNNs is extremely demanding in terms of computational resources. Also, for specific applications, the standard use of transfer learning also tends to require far more resources than what may be needed. Furthermore, the final systems tend to operate as black boxes that are difficult to interpret. The current thesis considers the problem of detecting faces from the AOLME video dataset. The AOLME dataset consists of a large video collection of group interactions that are recorded in unconstrained classroom environments. For the thesis, still image frames were extracted at every minute from 18 24-minute videos. Then, each video frame was divided into 9x5 blocks with 50x50 pixels each. For each of the 19440 blocks, the percentage of face pixels was set as ground truth. Face detection was then defined as a regression problem for determining the face pixel percentage for each block. For testing different methods, 12 videos were used for training and validation. The remaining 6 videos were used for testing. The thesis examines the impact of using the instantaneous phase for the AOLME block-based face detection application. For comparison, the thesis compares the use of the Frequency Modulation image based on the instantaneous phase, the use of the instantaneous amplitude, and the original gray scale image. To generate the FM and AM inputs, the thesis uses dominant component analysis that aims to decrease the training overhead while maintaining interpretability.Comment: Master Thesi

Guest Editorial Special Issue on Medical Imaging and Image Computing in Computational Physiology

International audienceThe January 2013 Special Issue of IEEE transactions on medical imaging discusses papers on medical imaging and image computing in computational physiology. Aslanid and co-researchers present an experimental technique based on stained micro computed tomography (CT) images to construct very detailed atrial models of the canine heart. The paper by Sebastian proposes a model of the cardiac conduction system (CCS) based on structural information derived from stained calf tissue. Ho, Mithraratne and Hunter present a numerical simulation of detailed cerebral venous flow. The third category of papers deals with computational methods for simulating medical imagery and incorporate knowledge of imaging physics and physiology/biophysics. The work by Morales showed how the combination of device modeling and virtual deployment, in addition to patient-specific image-based anatomical modeling, can help to carry out patient-specific treatment plans and assess alternative therapeutic strategies

AI in Medical Imaging Informatics: Current Challenges and Future Directions

This paper reviews state-of-the-art research solutions across the spectrum of medical imaging informatics, discusses clinical translation, and provides future directions for advancing clinical practice. More specifically, it summarizes advances in medical imaging acquisition technologies for different modalities, highlighting the necessity for efficient medical data management strategies in the context of AI in big healthcare data analytics. It then provides a synopsis of contemporary and emerging algorithmic methods for disease classification and organ/ tissue segmentation, focusing on AI and deep learning architectures that have already become the de facto approach. The clinical benefits of in-silico modelling advances linked with evolving 3D reconstruction and visualization applications are further documented. Concluding, integrative analytics approaches driven by associate research branches highlighted in this study promise to revolutionize imaging informatics as known today across the healthcare continuum for both radiology and digital pathology applications. The latter, is projected to enable informed, more accurate diagnosis, timely prognosis, and effective treatment planning, underpinning precision medicine