Hot-spot analysis for drug discovery targeting protein-protein interactions

Introduction: Protein-protein interactions are important for biological processes and pathological situations, and are attractive targets for drug discovery. However, rational drug design targeting protein-protein interactions is still highly challenging. Hot-spot residues are seen as the best option to target such interactions, but their identification requires detailed structural and energetic characterization, which is only available for a tiny fraction of protein interactions. Areas covered: In this review, the authors cover a variety of computational methods that have been reported for the energetic analysis of protein-protein interfaces in search of hot-spots, and the structural modeling of protein-protein complexes by docking. This can help to rationalize the discovery of small-molecule inhibitors of protein-protein interfaces of therapeutic interest. Computational analysis and docking can help to locate the interface, molecular dynamics can be used to find suitable cavities, and hot-spot predictions can focus the search for inhibitors of protein-protein interactions. Expert opinion: A major difficulty for applying rational drug design methods to protein-protein interactions is that in the majority of cases the complex structure is not available. Fortunately, computational docking can complement experimental data. An interesting aspect to explore in the future is the integration of these strategies for targeting PPIs with large-scale mutational analysis.This work has been funded by grants BIO2016-79930-R and SEV-2015-0493 from the Spanish Ministry of Economy, Industry and Competitiveness, and grant EFA086/15 from EU Interreg V POCTEFA. M Rosell is supported by an FPI fellowship from the Severo Ochoa program. The authors are grateful for the support of the the Joint BSC-CRG-IRB Programme in Computational Biology.Peer ReviewedPostprint (author's final draft

Structural Prediction of Protein–Protein Interactions by Docking: Application to Biomedical Problems

A huge amount of genetic information is available thanks to the recent advances in sequencing technologies and the larger computational capabilities, but the interpretation of such genetic data at phenotypic level remains elusive. One of the reasons is that proteins are not acting alone, but are specifically interacting with other proteins and biomolecules, forming intricate interaction networks that are essential for the majority of cell processes and pathological conditions. Thus, characterizing such interaction networks is an important step in understanding how information flows from gene to phenotype. Indeed, structural characterization of protein–protein interactions at atomic resolution has many applications in biomedicine, from diagnosis and vaccine design, to drug discovery. However, despite the advances of experimental structural determination, the number of interactions for which there is available structural data is still very small. In this context, a complementary approach is computational modeling of protein interactions by docking, which is usually composed of two major phases: (i) sampling of the possible binding modes between the interacting molecules and (ii) scoring for the identification of the correct orientations. In addition, prediction of interface and hot-spot residues is very useful in order to guide and interpret mutagenesis experiments, as well as to understand functional and mechanistic aspects of the interaction. Computational docking is already being applied to specific biomedical problems within the context of personalized medicine, for instance, helping to interpret pathological mutations involved in protein–protein interactions, or providing modeled structural data for drug discovery targeting protein–protein interactions.Spanish Ministry of Economy grant number BIO2016-79960-R; D.B.B. is supported by a predoctoral fellowship from CONACyT; M.R. is supported by an FPI fellowship from the Severo Ochoa program. We are grateful to the Joint BSC-CRG-IRB Programme in Computational Biology.Peer ReviewedPostprint (author's final draft

VIRTUAL TOURS FOR SMART CITIES: A COMPARATIVE PHOTOGRAMMETRIC APPROACH FOR LOCATING HOT-SPOTS IN SPHERICAL PANORAMAS

The paper aims to investigate the possibilities of using the panorama-based VR to survey data related to that set of activities for planning and management of urban areas, belonging to the Smart Cities strategies. The core of our workflow is to facilitate the visualization of the data produced by the infrastructures of the Smart Cities. A graphical interface based on spherical panoramas, instead of complex three-dimensional could help the user/citizen of the city to better know the operation related to control units spread in the urban area. From a methodological point of view three different kind of spherical panorama acquisition has been tested and compared in order to identify a semi-automatic procedure for locating homologous points on two or more spherical images starting from a point cloud obtained from the same images. The points thus identified allow to quickly identify the same hot-spot on multiple images simultaneously. The comparison shows how all three systems have proved to be useful for the purposes of the research but only one has proved to be reliable from a geometric point of view to identify the locators useful for the construction of the virtual tour

Assessing the perceived environment through crowdsourced spatial photo content for application to the fields of landscape and urban planning

Assessing information on aspects of identification, perception, emotion, and social interaction with respect to the environment is of particular importance to the fields of natural resource management. Our ability to visualize this type of information has rapidly improved with the proliferation of social media sites throughout the Internet in recent years. While many methods to extract information on human behavior from crowdsourced geodata already exist, this work focuses on visualizing landscape perception for application to the fields of landscape and urban planning. Visualization of people’s perceptual responses to landscape is demonstrated with crowdsourced photo geodata from Flickr, a popular photo sharing community. A basic, general method to map, visualize and evaluate perception and perceptual values is proposed. The approach utilizes common tools for spatial knowledge discovery and builds on existing research, but is specifically designed for implementation within the context of landscape perception analysis and particularly suited as a base for further evaluation in multiple scenarios. To demonstrate the process in application, three novel types of visualizations are presented: the mapping of lines of sight in Yosemite Valley, the assessment of landscape change in the area surrounding the High Line in Manhattan, and individual location analysis for Coit Tower in San Francisco. The results suggest that analyzing crowdsourced data may contribute to a more balanced assessment of the perceived landscape, which provides a basis for a better integration of public values into planning processes.:Contents 3 1 Introduction 7 1.1 Motivation 7 1.2 Literature review and conceptual scope 9 1.3 Terminology 11 1.4 Related research 12 1.5 Objectives 14 1.6 Methodology 16 1.7 Formal conventions 21 I. Part I: Conceptual framework 23 1.1 Visual perception 23 1.2 Theory and practice in landscape perception assessment 27 1.2.1 Expert valuation versus participation 27 1.2.2 Photography-based landscape perception assessment 32 1.2.2.1. Photo-based surveys 32 1.2.2.2. Photo-based Internet surveys 35 1.2.2.3. Photo-interviewing and participant photography 37 1.2.3 Conclusions 40 1.3 Conceptual approach 42 1.3.1 A framing theory: Distributed cognition 42 1.3.2 Description of the approach 46 1.3.3 Choosing the right data source 48 1.3.3.1. Availability of crowdsourced and georeferenced photo data 48 1.3.3.2. Suitability for analyzing human behavior and perception 51 1.3.4 Relations between data and the phenomenon under observation 55 1.3.4.1. Photo taking and landscape perception 55 1.3.4.2. User motivation in the context of photo sharing in communities 61 1.3.4.3. Describing and tagging photos: Forms of attributing meaning 66 1.3.5 Considerations for measuring and weighting data 70 1.3.6 Conclusions 77 II. Part II: Application example – Flickr photo analysis and evaluation of results 80 2.1 Software architecture 80 2.2 Materials and methods 86 2.2.1 Data retrieval, initial data structure and overall quantification 86 2.2.2 Global data bias 89 2.2.3 Basic techniques for filtering and classifying data 94 2.2.3.1. Where: photo locations 94 2.2.3.2. Who: user origin 96 2.2.3.3. When: time of photo taking 102 2.2.3.4. What: tag frequency 108   2.2.4 Methods for aggregating data 113 2.2.4.1. Clustering of photo locations 113 2.2.4.2. Clustering of tag locations 115 2.3 Application to planning: techniques for visualizing data 118 2.3.1 Introduction 118 2.3.2 Tag maps 121 2.3.2.1. Description of technique 121 2.3.2.2. Results: San Francisco and Berkeley waterfront 126 2.3.2.3. Results: Berkeley downtown and university campus 129 2.3.2.4. Results: Dresden and the Elbe Valley 132 2.3.2.5. Results: Greater Toronto Area and City of Toronto 136 2.3.2.6. Results: Baden-Württemberg 143 2.3.2.7. Summary 156 2.3.3 Temporal comparison for assessing landscape change 158 2.3.3.1. Description of technique 158 2.3.3.2. Results: The High Line, NY 159 2.3.3.3. Summary 160 2.3.4 Determining lines of sight and important visual connections 161 2.3.4.1. Description of technique 161 2.3.4.2. Results: Yosemite Valley 162 2.3.4.3. Results: Golden Gate and Bay Bridge 167 2.3.4.4. Results: CN Tower, Toronto 168 2.3.4.5. Summary 170 2.3.5 Individual location analysis 171 2.3.5.1. Description of technique 171 2.3.5.2. Results: Coit Tower, San Francisco 171 2.3.5.3. Results: CN Tower, Toronto 172 2.3.5.4. Summary 173 2.4 Quality and accuracy of results 175 2.4.1 Methodology 175 2.4.2 Accuracy of data 175 2.4.3 Validity and reliability of visualizations 178 2.4.3.1. Reliability 178 2.4.3.2. Validity 180 2.5 Implementation example: the London View Framework 181 2.5.1 Description 181 2.5.2 Evaluation methodology 183 2.5.3 Analysis 184 2.5.3.1. Landmarks 184 2.5.3.2. Views 192 2.5.4 Summary 199 III. Discussion 203 3.1 Application of the framework from a wider perspective 203 3.2 Significance of results 204 3.3 Further research 205   3.4 Discussion of workshop results and further feedback 206 3.4.1 Workshops at University of Waterloo and University of Toronto, Canada 206 3.4.2 Workshop at University of Technology Dresden, Germany 209 3.4.3 Feedback from presentations, discussions, exhibitions: second thoughts 210 IV. Conclusions 212 V. References 213 5.1 Literature 213 5.2 List of web references 228 5.3 List of figures 230 5.4 List of tables 234 5.5 List of maps 235 5.6 List of appendices 236 VI. Appendices 237  Als Wahrnehmung wird der Bewusstseinsprozess des subjektiven Verstehens der Umwelt bezeichnet. Grundlage für diesen Prozess ist die Gewinnung von Informationen über die Sinne, also aus visuellen, olfaktorischen, akustischen und anderen Reizen. Die Wahrnehmung ist aber auch wesentlich durch interne Prozesse beeinflusst. Das menschliche Gehirn ist fortlaufend damit beschäftigt, sowohl bewusst als auch unbewusst Sinneswahrnehmungen mit Erinnerungen abzugleichen, zu vereinfachen, zu assoziieren, vorherzusagen oder zu vergleichen. Aus diesem Grund ist es schwierig, die Wahrnehmung von Orten und Landschaften in Planungsprozessen zu berücksichtigen. Jedoch wird genau dies von der Europäischen Landschaftskonvention gefordert, die Landschaft als einen bestimmten Bereich definiert, so wie er von Besuchern und Einwohnern wahrgenommen wird (“as a zone or area as perceived by local people or visitors”, ELC Art. 1, Abs. 38). Während viele Fortschritte und Erkenntnisse, zum Beispiel aus den Kognitionswissenschaften, heute helfen, die Wahrnehmung einzelner Menschen zu verstehen, konnte die Stadt- und Landschaftsplanung kaum profitieren. Es fehlt an Kenntnissen über das Zusammenwirken der Wahrnehmung vieler Menschen. Schon Stadtplaner Kevin Lynch beschäftigte dieses gemeinsame, kollektive ‚Bild‘ der menschlichen Umwelt ("generalized mental picture", Lynch, 1960, p. 4). Seitdem wurden kaum nennenswerte Fortschritte bei der Erfassung der allgemeinen, öffentlichen Wahrnehmung von Stadt- und Landschaft erzielt. Dies war Anlass und Motivation für die vorliegende Arbeit. Eine bisher in der Planung ungenutzte Informationsquelle für die Erfassung der Wahrnehmung vieler Menschen bietet sich in Form von crowdsourced Daten (auch ‚Big Data‘), also großen Mengen an Daten die von vielen Menschen im Internet zusammengetragen werden. Im Vergleich zu konventionellen Daten, zum Beispiel solchen die durch Experten erhoben werden und durch öffentliche Träger zur Verfügung stehen, eröffnet sich durch crowdsourced Daten eine bisher nicht verfügbare Quelle für Informationen, um die komplexen Zusammenhänge zwischen Raum, Identität und subjektiver Wahrnehmung zu verstehen. Dabei enthalten crowdsourced Daten lediglich Spuren menschlicher Entscheidungen. Aufgrund der Menge ist es aber möglich, wesentliche Informationen über die Wahrnehmung derer, die diese Daten zusammengetragen haben, zu gewinnen. Dies ermöglicht es Planern zu verstehen, wie Menschen ihre unmittelbare Umgebung wahrnehmen und mit ihr interagieren. Darüber hinaus wird es immer wichtiger, die Ansichten Vieler in Planungsprozessen zu berücksichtigen (Lynam, De Jong, Sheil, Kusumanto, & Evans, 2007; Brody, 2004). Der Wunsch nach öffentlicher Beteiligung sowie die Anzahl an beteiligten Stakeholdern nehmen dabei konstant zu. Durch das Nutzen dieser neuen Informationsquelle bietet sich eine Alternative zu herkömmlichen Ansätzen wie Umfragen, die genutzt werden um beispielsweise Meinungen, Positionen, Werte, Normen oder Vorlieben von bestimmten sozialen Gruppen zu messen. Indem es crowdsourced Daten erleichtern, solch soziokulturelle Werte zu bestimmen, können die Ergebnisse vor allem bei der schwierigen Gewichtung gegensätzlicher Interessen und Ansichten helfen. Es wird die Ansicht geteilt, dass die Nutzung von crowdsourced Daten, indem Einschätzungen von Experten ergänzt werden, letztendlich zu einer faireren, ausgeglichenen Berücksichtigung der Allgemeinheit in Entscheidungsprozessen führen kann (Erickson, 2011, p.1). Eine große Anzahl an Methoden ist bereits verfügbar, um aus dieser Datenquelle wichtige landschaftsbezogene Informationen auszulesen. Beispiele sind die Bewertung der Attraktivität von Landschaften, die Bestimmung der Bedeutung von Sehenswürdigkeiten oder Wahrzeichen, oder die Einschätzung von Reisevorlieben von Nutzergruppen. Viele der bisherigen Methoden wurden jedoch als ungenügend empfunden, um die speziellen Bedürfnisse und das breite Spektrum an Fragestellungen zur Landschaftswahrnehmung in Stadt- und Landschaftsplanung zu berücksichtigen. Das Ziel der vorliegenden Arbeit ist es, praxisrelevantes Wissen zu vermitteln, welches es Planern erlaubt, selbstständig Daten zu erforschen, zu visualisieren und zu interpretieren. Der Schlüssel für eine erfolgreiche Umsetzung wird dabei in der Synthese von Wissen aus drei Kategorien gesehen, theoretische Grundlagen (1), technisches Wissen zur Datenverarbeitung (2) sowie Kenntnisse zur grafischen Visualisierungen (3). Die theoretischen Grundlagen werden im ersten Teil der Arbeit (Part I) präsentiert. In diesem Teil werden zunächst Schwachpunkte aktueller Verfahren diskutiert, um anschließend einen neuen, konzeptionell-technischen Ansatz vorzuschlagen der gezielt auf die Ergänzung bereits vorhandener Methoden zielt. Im zweiten Teil der Arbeit (Part II) wird anhand eines Datenbeispiels die Anwendung des Ansatzes exemplarisch demonstriert. Fragestellungen die angesprochen werden reichen von der Datenabfrage, Verarbeitung, Analyse, Visualisierung, bis zur Interpretation von Grafiken in Planungsprozessen. Als Basis dient dabei ein Datenset mit 147 Millionen georeferenzierte Foto-Daten und 882 Millionen Tags der Fotoaustauschplatform Flickr, welches in den Jahren 2007 bis 2015 von 1,3 Millionen Nutzern zusammengetragen wurde. Anhand dieser Daten wird die Entwicklung neuer Visualisierungstechniken exemplarisch vorgestellt. Beispiele umfassen Spatio-temporal Tag Clouds, eine experimentelle Technik zur Generierung von wahrnehmungsgewichteten Karten, die Visualisierung von wahrgenommenem Landschaftswandel, das Abbilden von wahrnehmungsgewichteten Sichtlinien, sowie die Auswertung von individueller Wahrnehmung von und an bestimmten Orten. Die Anwendung dieser Techniken wird anhand verschiedener Testregionen in den USA, Kanada und Deutschland für alle Maßstabsebenen geprüft und diskutiert. Dies umfasst beispielsweise die Erfassung und Bewertung von Sichtlinien und visuellen Bezügen in Yosemite Valley, das Monitoring von wahrgenommenen Veränderungen im Bereich der High Line in New York, die Auswertung von individueller Wahrnehmung für Coit Tower in San Francisco, oder die Beurteilung von regional wahrgenommenen identitätsstiftenden Landschaftswerten für Baden-Württemberg und die Greater Toronto Area (GTA). Anschließend werden Ansätze vorgestellt, um die Qualität und Validität von Visualisierungen einzuschätzen. Abschließend wird anhand eines konkreten Planungsbeispiels, des London View Management Frameworks (LVMF), eine spezifische Implementation des Ansatzes und der Visualisierungen kurz aufgezeigt und diskutiert. Mit der Arbeit wird vor allem das breite Potential betont, welches die Nutzung von crowdsourced Daten für die Bewertung von Landschaftswahrnehmung in Stadt- und Landschaftsplanung bereithält. Insbesondere crowdsourced Fotodaten werden als wichtige zusätzliche Informationsquelle gesehen, da sie eine bisher nicht verfügbare Perspektive auf die allgemeine, öffentliche Wahrnehmung der Umwelt ermöglichen. Während der breiteren Anwendung noch einige Grenzen gesetzt sind, können die vorgestellten experimentellen Methoden und Techniken schon wichtige Aufschlüsse über eine ganze Reihe von wahrgenommenen Landschaftswerten geben. Auf konzeptioneller Ebene stellt die Arbeit eine erste Grundlage für weitere Forschung dar. Bevor jedoch eine breite Anwendung in der Praxis möglich ist, müssen entscheidende Fragen gelöst werden, beispielsweise zum Copyright, zur Definition von ethischen Standards innerhalb der Profession, sowie zum Schutz der Privatsphäre Beteiligter. Längerfristig wird nicht nur die Nutzung der Daten als wichtig angesehen, sondern auch die Erschließung der essentiellen Möglichkeiten dieser Entwicklung zur besseren Kommunikation mit Auftraggebern, Beteiligten und der Öffentlichkeit in Planungs- und Entscheidungsprozessen.:Contents 3 1 Introduction 7 1.1 Motivation 7 1.2 Literature review and conceptual scope 9 1.3 Terminology 11 1.4 Related research 12 1.5 Objectives 14 1.6 Methodology 16 1.7 Formal conventions 21 I. Part I: Conceptual framework 23 1.1 Visual perception 23 1.2 Theory and practice in landscape perception assessment 27 1.2.1 Expert valuation versus participation 27 1.2.2 Photography-based landscape perception assessment 32 1.2.2.1. Photo-based surveys 32 1.2.2.2. Photo-based Internet surveys 35 1.2.2.3. Photo-interviewing and participant photography 37 1.2.3 Conclusions 40 1.3 Conceptual approach 42 1.3.1 A framing theory: Distributed cognition 42 1.3.2 Description of the approach 46 1.3.3 Choosing the right data source 48 1.3.3.1. Availability of crowdsourced and georeferenced photo data 48 1.3.3.2. Suitability for analyzing human behavior and perception 51 1.3.4 Relations between data and the phenomenon under observation 55 1.3.4.1. Photo taking and landscape perception 55 1.3.4.2. User motivation in the context of photo sharing in communities 61 1.3.4.3. Describing and tagging photos: Forms of attributing meaning 66 1.3.5 Considerations for measuring and weighting data 70 1.3.6 Conclusions 77 II. Part II: Application example – Flickr photo analysis and evaluation of results 80 2.1 Software architecture 80 2.2 Materials and methods 86 2.2.1 Data retrieval, initial data structure and overall quantification 86 2.2.2 Global data bias 89 2.2.3 Basic techniques for filtering and classifying data 94 2.2.3.1. Where: photo locations 94 2.2.3.2. Who: user origin 96 2.2.3.3. When: time of photo taking 102 2.2.3.4. What: tag frequency 108   2.2.4 Methods for aggregating data 113 2.2.4.1. Clustering of photo locations 113 2.2.4.2. Clustering of tag locations 115 2.3 Application to planning: techniques for visualizing data 118 2.3.1 Introduction 118 2.3.2 Tag maps 121 2.3.2.1. Description of technique 121 2.3.2.2. Results: San Francisco and Berkeley waterfront 126 2.3.2.3. Results: Berkeley downtown and university campus 129 2.3.2.4. Results: Dresden and the Elbe Valley 132 2.3.2.5. Results: Greater Toronto Area and City of Toronto 136 2.3.2.6. Results: Baden-Württemberg 143 2.3.2.7. Summary 156 2.3.3 Temporal comparison for assessing landscape change 158 2.3.3.1. Description of technique 158 2.3.3.2. Results: The High Line, NY 159 2.3.3.3. Summary 160 2.3.4 Determining lines of sight and important visual connections 161 2.3.4.1. Description of technique 161 2.3.4.2. Results: Yosemite Valley 162 2.3.4.3. Results: Golden Gate and Bay Bridge 167 2.3.4.4. Results: CN Tower, Toronto 168 2.3.4.5. Summary 170 2.3.5 Individual location analysis 171 2.3.5.1. Description of technique 171 2.3.5.2. Results: Coit Tower, San Francisco 171 2.3.5.3. Results: CN Tower, Toronto 172 2.3.5.4. Summary 173 2.4 Quality and accuracy of results 175 2.4.1 Methodology 175 2.4.2 Accuracy of data 175 2.4.3 Validity and reliability of visualizations 178 2.4.3.1. Reliability 178 2.4.3.2. Validity 180 2.5 Implementation example: the London View Framework 181 2.5.1 Description 181 2.5.2 Evaluation methodology 183 2.5.3 Analysis 184 2.5.3.1. Landmarks 184 2.5.3.2. Views 192 2.5.4 Summary 199 III. Discussion 203 3.1 Application of the framework from a wider perspective 203 3.2 Significance of results 204 3.3 Further research 205   3.4 Discussion of workshop results and further feedback 206 3.4.1 Workshops at University of Waterloo and University of Toronto, Canada 206 3.4.2 Workshop at University of Technology Dresden, Germany 209 3.4.3 Feedback from presentations, discussions, exhibitions: second thoughts 210 IV. Conclusions 212 V. References 213 5.1 Literature 213 5.2 List of web references 228 5.3 List of figures 230 5.4 List of tables 234 5.5 List of maps 235 5.6 List of appendices 236 VI. Appendices 237

LAV@HAZARD: A Web-Gis interface for volcanic hazard assessment

Satellite data, radiative power of hot spots as measured with remote sensing, historical records, on site geological surveys, digital elevation model data, and simulation results together provide a massive data source to investigate the behavior of active volcanoes like Mount Etna (Sicily,Italy) over recent times. The integration of these eterogeneous data into a coherent visualization framework is important for their practical exploitation. It is crucial to fill in the gap between experimental and numerical data, and the direct human perception of their meaning. Indeed, the people in charge of safety planning of an area need to be able to quickly assess hazards and other relevant issues even during critical situations. With this in mind, we developed LAV@HAZARD, a web-based geographic information system that provides an interface for the collection of all of the products coming from the LAVA project research activities. LAV@HAZARD is based on Google Maps application programming interface, a choice motivated by its ease of use and the user-friendly interactive environment it provides. In particular, the web structure consists of four modules for satellite applications (time-space evolution of hot spots, radiant flux and effusion rate), hazard map visualization, a database of ca. 30,000 lava-flow simulations, and real-time scenario forecasting by MAGFLOW on Compute Unified Device Architecture

Detecting and Visualizing Observation Hot-Spots in Massive Volunteer-Contributed Geographic Data Across Spatial Scales Using GPU-Accelerated Kernel Density Estimation

Volunteer-contributed geographic data (VGI) is an important source of geospatial big data that support research and applications. A major concern on VGI data quality is that the underlying observation processes are inherently biased. Detecting observation hot-spots thus helps better understand the bias. Enabled by the parallel kernel density estimation (KDE) computational tool that can run on multiple GPUs (graphics processing units), this study conducted point pattern analyses on tens of millions of iNaturalist observations to detect and visualize volunteers’ observation hot-spots across spatial scales. It was achieved by setting varying KDE bandwidths in accordance with the spatial scales at which hot-spots are to be detected. The succession of estimated density surfaces were then rendered at a sequence of map scales for visual detection of hot-spots. This study offers an effective geovisualization scheme for hierarchically detecting hot-spots in massive VGI datasets, which is useful for understanding the pattern-shaping drivers that operate at multiple spatial scales. This research exemplifies a computational tool that is supported by high-performance computing and capable of efficiently detecting and visualizing multi-scale hot-spots in geospatial big data and contributes to expanding the toolbox for geospatial big data analytics

Taking the urban tourist activity pulse through digital footprints

An insight on urban tourism-related phenomena is provided in this study by analysing open and volunteered user generated content. A reference framework method is proposed and applied to an illustrative case study to meet a twofold objective: to identify Tourist Activity Centre – TAC – areas based on their functional character – sightseeing, shopping, eating and nightlife; and, to obtain an up-to-date fine-grain characterization of the most dynamic zones in an urban context. Instasights Heatmaps and data from Location Based Social Networks – Foursquare, Google Places, Twitter and Airbnb – were used to depict tourist urban activity. This reproducible method transcends Instasights generic visualization of popular areas by exploiting the benefits of overlapping LBSN data sources. This method facilitates a granular analysis of tourism-related places of interest and makes headway in bridging the gap between traditional approaches and user preferences, revealed through digital footprints, for urban analysis. The results indicate the potential of this method as a complementary tool for urban planning decision-making.This research was funded by the Vice-rectorate of Research and Knowledge Transfer of the University of Alicante, in the context of the Program for the promotion of R+D+I. This work was developed within the scope of the research project entitled: "[LIVELYCITY] Interdisciplinary methods for the study of the city through geolocated social networks", reference GRE18-19

Spatial Pattern and Hotspots of Urban Rail Public Transport to Public Access Using Geospatial Techniques in Selangor, Malaysia

Currently, the trends in urban public transport have been changing over the years in developing countries for mobilization and accessibility development. Urban public transportation systems are the most popular in Selangor State, including big cities such as the Klang Valley Region. Objective measures of spatial pattern and hotspots have been used to understand how urban public transport development relate to open access. This method relies on specific spatial information and available web-based tool that shows the pattern primarily based on given vicinity and statistics connectivity. To date, several studies have finished tested in developed countries. In this study, we use Geographic Information Systems to analyse and consider hotspots identification precisely and efficaciously. Therefore, in this paper, we focus on two types of point sample evaluations – Gi* hot spot and point density analysis evaluation as statistical operations. Public rail transport was evaluated as a validation to describe the percentage of distribution of open access. The final result, GIS mapping capabilities to show that GIS's technology offers to the variation of urban public transport relate to public services, is to create maps and spatial interpretations